• 대한수의학회지
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• 제47권2호
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• pp.157-162
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• 2007

Lactic acid bacteria (LAB) has been regarded as a useful microorganism and tried to manipulate plasmid DNA for increasing the usefulness. Although several methods have been developed to isolate plasmid DNA from Escherichia coli (E. coli), these methods were not sufficient to apply to LAB with exception of O'Sullivan's lysis method. So, we evaluated plasmid DNA extraction from LAB using general E. coli preparation methods and tried to improve the extraction yield and DNA purity by modifying O'Sullivan's alkaline lysis method. To improve the extraction yield, salt and carrier were added to precipitant and those were incubated at $-70{^{\circ}C}$. Only incubation at $-70{^{\circ}C}$ was the effective method of those modifications. Purity of plasmid DNA was improved by two times of each centrifugation and phenol/chloroform extraction. However, DNA was damaged by twice extraction with phenol/chloroform. Also, exclusion of ethidium bromide showed negative effect to purity. Additionally, it was recommended that improvement of the extraction yield may be due to centrifugation at high speed for more time and to dissolving complete DNA pellet before addition of 7.5 M ammonium acetate. Extraction using this modification produced higher quality of plasmid DNA.

• 한국주거학회논문집
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• 제20권5호
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• pp.23-30
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• 2009

In case of national rental apartment, habitability is generally evaluated low comparing with typical apartments. So, researches and investment are required to increase the residential environment. In this point of view, present study aims to suggest the basic data for improvement of homeless masses' dwelling level through economic feasibility analysis. Firstly national rental apartment, which habitability is considered to be low in the point of environment-friendly, welfare, and convenience, is chosen as objective. Secondly environment-friendly project items including project flow were set up, and then basic model was formed to evaluate economic feasibility through priority survey to residents. In result, priority for two items among six different proposals were higher than the others. This result could be useful when trying to increase the dwelling level of national rental apartment being planned to construct in future.

• 대한안전경영과학회지
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• 제19권2호
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• pp.117-133
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• 2017

The purpose of this study is to consider the relation between the deciding factors of local identity and local image improvement. To achieve this purpose, the deciding factors of local identity have been set as the following four: historical identity, cultural identity, (nature) scenery identity, and industrial identity. The writer has established a model to represent the relation between these factors and the local image improvement, namely 'Visitor Satisfaction' and 'Revisit Intentions', and framed a hypothesis for empirical verification. The study results are as follows: First, the deciding factors of local identity which this study addressed are empirically proven to have a significant effect on the visitor satisfaction and the intention to revisit in which is it likely that the greater the impact of the deciding factors, the higher the visitor satisfaction and revisit intentions would be. Second, the deciding factors of local identity, it appears that the 'historical identity' factor had the largest impact on the visitor satisfaction, and the 'cultural identity' factor had the largest impact on the revisit intentions. Therefore, of the four deciding factors, the 'historical identity' should be considered the highest priority to increase the visitor satisfaction, and the 'cultural identity' should be considered the highest priority to increase the revisit intentions. Third, based on the verification of the relation between the visitor satisfaction and revisit intentions, it can be presumed that the greater the visitor satisfaction, the higher the revisit intentions would be.

• 수산해양기술연구
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• 제57권4호
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• pp.316-333
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• 2021

In order to understand basic data for improving the fishing system and fishing vessel structure in coastal improved stow net fishery, a questionnaire survey and on-site hearing were conducted from May 10 to June 11, 2019 to analyze opinions on the improvement of operation status and fishing vessel structure. The questionnaire survey consisted of ten questions on the operation status of coastal improved stow net fishery and six questions on the improvement of fishing vessel structure, and the results of each question were analyzed by the region, the captain's age, the captain's career and the age of fishing vessel. As a result of analyzing opinions on the operation status of the coastal improved stow net fishery, it was found that the average time required for casting net was 32.8 to 33.0 minutes and that the average time required for hauling net was 41.0 to 42.2 minutes which took 10 to 12 minutes more than for casting net. The most important work requiring improvement during fishing operation (the first priority) were 'hauling net operation,' 'readjustment and storage of fishing gear,' and 'fish handling' and the hardest factor in fishing management were in the order of 'reduction of catch,' 'labor shortage' and 'rising labor costs.' The most institutional improvement that is most needed in coastal improved stow net fishery was an 'using fine mesh nets.' Most of the respondent to the questions on the experience in hiring foreign crews was 'either hiring or willing to hire foreign crews,' and the average number of foreign crews employed was found to be 2.3 to 2.4 persons. The most important reason for hiring (or considering employment) foreign crews was 'high labor costs.' The degree of communication with foreign crews during fishing operation were 'moderate' or 'difficult to direct work.' The most important problem in hiring foreign crews (the first priority) was an 'illegal departure.' As the survey results on the opinion of structural improvement of coastal improved stow net fishing vessel, the degree of satisfaction with fishing vessel structure related to fishing operation was found to be somewhat unsatisfactory, with an average of 3.3 points on a five-point scale. The inconvenient structure of fishing vessel in possession (the first priority), the space needed most for the construction of new fishing vessel (the first priority) and the space considered important for the construction of new fishing vessel (the first prioprity) was a 'fish warehouse.' The most preferred equipment for the construction of new fishing vessel were 'engine operation monitoring' and 'navigation safety devices.' The average size (tonnage class), the average horse power and the average total length of fishing vessel for proper profit and safety fishing operation was between 13.8 and 14.0 tonnes, 808.3 to 819.5 H.P. and 23.4 to 23.5 meters, respectively. The results of the operation status of coastal improved stow net fishery and the requirement for improving the fishing vessel structure are expected to be provided as basic data for reference when we build or improve the fishing vessel.

• 한국간호교육학회지
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• 제5권1호
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• pp.133-141
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• 1999

Recently there are increasing concerns on quality improvement activities related to difficult economic situations, more competitive environment, health professional's emphasis on quality, and customer's needs in health care. The purpose of this study was to identify educational needs in the quality improvement for staff nurses. Study setting was an acute care hospital having more than 1000 bed in Seoul, Korea. The subjects were 40 staff nurses who participated in the first Quality Assurance(QA) inservice education. Data were collected by self-administered questionnaire which consisted of four parts : QA knowledge and attitude(ten items, by five Likert scale), contents of QA education(ten items, by setting the priority), evaluation of the program(structure, time allocation, place, educational method), and general characteristics of respondents (age, duration to work for hospitals etc.) The response rate was 85%(34/40), Most had positive perspective and attitude about QI/QA activities, but 9% had negative impression and knowledge about this activities. Also they'd like to know the plan of hospital-level QI/QA activities, QI/QA current practice, general hospital system, the role of QA specialist and so on. Consequently, for building the quality improvement activities that is customer-focused, coordinated, outcome-oriented, resource-efficient, collaborative in Korea, at first the education about philosophy, theory, and implementation process of QI/QA should be conducted, and then that on QI/QA terminology, quality indicators development, the analysis and presentation of quality-related data and so forth be followed.

• 한국ITS학회 논문지
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• 제20권3호
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• pp.20-33
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• 2021

현대 사회는 한정된 도로로 증가하는 교통 수요를 처리하기 위해 대중교통 장려 정책을 꾸준히 실시하고 있다. 버스의 경쟁력을 확보하기 위해 전국적으로 간선급행버스체계(BRT) 설치가 확대함에 따라 버스의 신속성 보장을 위한 우선 신호에 대한 기대가 높아지고 있다. 그러나 일부 버스 운영 측면을 고려하지 않은 BRT 정류장 형태는 버스의 정지횟수를 증가시켜 버스 우선 신호 적용 효과를 감소시킬 수 있다. 이에 본 연구는 세종시 한누리대로를 분석 대상지로 하여 BRT 정류장 형태에 따른 버스 우선 신호 효과를 분석함으로써 버스의 운영 효율을 높이기 위한 정류장 형태에 대해 제언하고자 한다. 버스 우선 신호는 일반 차량과 버스의 양방향 연동폭을 최대화하는 기법을 적용하였다. 적용 결과 버스의 이중 정차를 야기하는 정류장에서 버스 우선 신호의 효과가 급격히 감소하였으며 이를 개선 후 우선 신호의 효율이 상당히 증가하였다. 본 연구의 결과는 향후 신도시에 BRT 정류장 설계 시 교통운영 측면을 고려한 올바른 정류장 형태의 근거자료로써 활용이 가능할 것으로 기대된다.

• 한국건설관리학회:학술대회논문집
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• 한국건설관리학회 2006년도 정기학술발표대회 논문집
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• pp.698-701
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• 2006

연약지반 상에 구조물의 안정 및 침하 문제를 해결하기 위해 다양한 연약지반 개량공법들이 사용되고 있으며, 계속해서 새로운 공법들이 검증과정을 거쳐 소개되고 있다. 이처럼 다양한 공법들 중에서 해당 공사에 가장 적합한 공법을 선정하는 것은 매우 중요한 일이다. 본 연구에서는 의사결정시 사용되는 영향요소들의 중요도를 정량화시켜 가중치와 우선순위를 도출함으로써 연약지반 개량공법 선정 과정에서 기술적 ${\cdot}$ 경제적 항목에 대한 체계적인 기준을 제시하고자 하였다.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2012년도 추계 학술논문 발표대회
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• pp.287-289
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• 2012

The building construction industry of Korea was going through difficulties in improving productivity and competitiveness in international market. Therefore we expected to see the improvement in productivity by applying six-sigma based approach to general reinforced concrete construction work. As a result of applying DMAIC method, one of 6 sigma tools, we found that the most effective inspection items are 'Gap of forms' and 'status of clean'. We could see from this case study that the percentage of additional work days has decreased to 37.7% as a result of applying six-sigma based approach to reinforced concrete construction.

• 한국어학
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• 제79권
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• pp.145-169
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• 2018

The purpose of this paper is to review critically the validity and relevance of the pan-national language culture improvement movement, Annyeong! Urimal(Hello! Our Language) implemented for the last three years as part of the government-driven projects, and to seek for improvement direction or fundamental reorientation of this movement. In order to accomplish this purpose, this paper firstly points out abstract and conventional aspects of the diagnosis for background and the purpose of projects, and suggests that this movement focuses on verbal abuse rather than language destruction and pays attention to positive aspects of newly rising language use. Then, with reference to main agent, this paper argues that the government must lead actively formation of voluntary nongovernmental agent with indirect financial and institutional support. Thirdly, it is suggested that public language should be the top priority target sphere and the language used by the leaders of society and the older generation should be also included in the target spheres of this movement. Finally, this paper indicates that enlightenment/public relation projects end often in one time events, imposing unilaterally the normative contents, and also emphasizes the importance of survey/research projects and deliberation/evaluation projects.

• 한국안전학회지
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• 제30권3호
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• pp.123-134
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• 2015

This study was conducted with the following three study objectives. First, effects of safety awareness level of lab researchers to the improvement of safety culture in the organization Second, effects of organizational safety behaviors to the improvement of safety culture Third, test of mediating effects of organizational safety behaviors in the relationship between safety awareness level and the improvement of safety culture. The results show that organizational safety behavior is an indispensable factor for the improvement level of safety culture. Especially, the factors in safety training activities, safety compliance and management system are mediating variables which affect the safety awareness level and improvement level of safety culture, which shows these variables are very important factors in reducing safety accidents through the improvement of safety culture. Therefore, safety behaviors in the organization should be considered with priority. If the organization leads to improve safety awareness through regular safety training and rewards and punishes according to the test results, safety awareness could be improved. This study was conducted to identify the necessary factors to improve the overall safety culture in the organization and contribute to the diffusion of safety culture by improving the safety training awareness of the researchers.