• Mycobiology
• /
• v.43 no.3
• /
• pp.280-287
• /
• 2015

In this study, transcriptome analysis of twelve laccase genes in Pleurotus ostreatus revealed that their expression was differentially regulated at different developmental stages. Lacc5 and Lacc12 were specifically expressed in fruiting bodies and primordia, respectively, whereas Lacc6 was expressed at all developmental stages. Lacc1 and Lacc3 were specific to the mycelial stage in solid medium. In order to investigate their biochemical characteristics, these laccases were heterologously expressed in Pichia pastoris using the pPICHOLI-2 expression vector. Expression of the laccases was facilitated by intermittent addition of methanol as an inducer and sole carbon source, in order to reduce the toxic effects associated with high methanol concentration. The highest expression was observed when the recombinant yeast cells were grown for 5 days at $15^{\circ}C$ with intermittent addition of 1% methanol at a 12-hr interval. Investigation of enzyme kinetics using 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) as a substrate revealed that the primordium-specific laccase Lacc12 was 5.4-fold less active than Lacc6 at low substrate concentration with respect to ABTS oxidation activity. The optimal pH and temperature of Lacc12 were 0.5 pH units and $5^{\circ}C$higher than those of Lacc6. Lacc12 showed maximal activity at pH 3.5 and $50^{\circ}C$, which may reflect the physiological conditions at the primordiation stage.

• Journal of Life Science
• /
• v.16 no.7 s.80
• /
• pp.1141-1147
• /
• 2006

This study was carried out to establish a reproducible culture system for callus formation and adventitious shoot development from young stem segments of Vigna radinta, and histological work for orgin of callus tissue and adventitious shoot. Induction of callus from young stem explants of Vigna radiata was very effective on MS inorganic salts supplemented with 0.5 mg/L 2,4-D and 1.0 mg/L kinetin. For the adventitious shoot regeneration from the callus tissues, the hormone combination of 0.75 mg/L NAA, 1.5 mg/L kinetin and MS salts resulted in about 21% efficiency. Histological examination showed that callus tissues originated from out-growths by callus cambium rings with do novo meristematic activities, which were localized at the outside of the vascular cambium. Adventitious shoots were developed from shoot apical meristem originated from the surface of callus masses. The shoot apical meristem produced leaf primordium, which then became leaf.

• The Korean Journal of Mycology
• /
• v.47 no.3
• /
• pp.233-240
• /
• 2019

This study was performed to develop a useful method for substrate pH adjustment in bottle cultivation of Grifola frondosa. Four types of organic acid solution-citric acid, malic acid, tartaric acid, and succinic acid-were added at 0.5% to 3.0% (w/v) into the substrate. The pH after the organic acid treatments ranged from 3.8 to 4.8 for the mycelial growth of G. frondosa after sterilization. Supplementation with succinic acid at 0.5% resulted in a shorter cultivation period and higher consistent yields compared to other organic acid treatments. These results indicate that the technique we developed is beneficial for higher production of G. frondosa using bottle cultivation.

• Journal of Korean Society of Forest Science
• /
• v.64 no.1
• /
• pp.33-41
• /
• 1984

This study was conducted to examine the physiology of pine mushroom mycelia cultured with various media for artificial culture of pine mushroom. The results obtained were as follows: 1) Among the various media, the medium composed of honey, boiled pine mushroom and soil extract fluid, fibrous root extract fluid, dry yeast, $KH_2PO_4$ inositol, folic acid, and biotin was the best for the growth of pine mushroom mycelium. 2) The optimum temperature for germinating pine mushroom spore and for culturing pine mushroom mycelium, was $24^{\circ}C$ and the optimum pH was 4.5. 3) There was no significant difference in growth between the mycelium separated from the tissue of pine mushroom sporophore and that separated from the spore. 4) No noticeable effect was found on the growth if such salts as $ZnSO_4$, $MnSO_4$, $MgSO_4$, $CaCl_2$ and ferric citrate were added to the Hamada's medium. 5) The addition of fibrous root extract promoted the growth of pine mushroom mycelium. 6) As a carbon source of artificial media, honey was more effective than glucose. 7) The culture infiltration of Mortierlla growing often in Fairy Ring was good for the growth of mycelium compared with the control. 8) The addition of fibrous root extract, inositol, biotin, and folic acid to artificial culture media was greatly effective in growth. When the temperature was lowered $19^{\circ}C$ after mycelium has appeared, the formation of primordium was observed.

• Parasites, Hosts and Diseases
• /
• v.24 no.2
• /
• pp.109-114
• /
• 1986

In order to observe the growth and development of Fibricola seoulensis metacercariae, the tadpoles of Rana nigromaculata were experimentally infected with the cercariae. The meta cercariae of various developmental stages were recovered from the tadpoles after 2 to 65 days of infection. They were prepared for morphological observation, and were given orally to mice to observe their infectivity. The following results were obtained. 1. All of the tadpoles exposed to the cercariae were observed to harbour the larvae in their abdominal cavity. 2. The young metacercariae of 2 days after infection were $121.1{\mu}m$ long and $63.3{\mu}m$ wide. They grew linearly for the first 14 days to be $262.0{\mu}m$ long and $166.4{\mu}m$ wide. Thereafter, no more growth recognized until 65 days. 3. The larvae of 2 days old were similar with cercarial body and had 2 suckers, a pharynx, 2 ceca and a primordium of germ cells but no tribocytic organ. On the 8th day, they had tribocytic organ, and their morphology resembled that of mature metacercariae. 4. The metacercariae younger than 10 days could not infect the mice. Only the metacercariae older than 14 days had infectivity. The recovery rates increased by the age of metacercariae from 19.0% in 14 days old to 70.0% in 40 days old. Above findings indicate that the tadpole is indispensable for metacercarial development and it needs at least 2 weeks for maturation. The tadpole is a pivotal host in the life cycle of F. seoulensis for connection between the snail and the frog.

• Journal of Mushroom
• /
• v.14 no.2
• /
• pp.39-43
• /
• 2016

In this study, we observed differences in the fruit body genesis in with seasonal changes. The mushrooms generated different spines even when under same environmental conditions in equivalent growth chambers during different seasons. The growth chamber conditions were set at $18^{\circ}$, 1000 ppm CO and 95% humidity for uniform primordium formation. Following this, the humidity was reduced to 85% during growth and development. The characteristics of the fruit body were measured: yield (weight), length (size), hardness, and chromaticity. Each measure was relatively compared owing standard data for. We investigated the environmental factors to assess the cause of variation in fruit body. Change in temperature and humidity were especially focused on, apart from climate that can affect mushroom growth. It was observed that temperature and humidity both influence fruit body growth. Not the absolute value of the elevation, there was a change of climate which the temperature is lowered and lowered the humidity can be assumed that the changes in the environmental conditions inside the growth chamber the mushroom growth.

• Journal of Life Science
• /
• v.17 no.9 s.89
• /
• pp.1272-1277
• /
• 2007

Comparison of fruit bodies of Pleurotus ostreatus cultivar chunchu No .2 grown on the sawdust, rice straw and cotton waste substrates revealed differences in the pattern of differentiation of hyphal compartments. Required period for primordium induction of fruit bodies grown on sawdust substrate was 13 days. Physical structure shown as hardness of stipes grown on the sawdust substrate, fruit bodies were harden than control. Pileocystidia were well developed on the surface of pileus in the fruit body cultivated on rice straw. Microstructures of fruit body grown on the sawdust and cotton wastes substrates shown fast-discharge of basidiospore and sytoms ageing. Hyphae of fruit bodies formed on sawdust substrate had less stainable cytoplasmic material and many more vacuoles than hyphae of fruit bodies formed on synthetic substrate with 50% of pine sawdust, 30% of cotton seed hull and 20 of beet pulp(control).

• The Korean Journal of Mycology
• /
• v.36 no.1
• /
• pp.51-57
• /
• 2008

To develop artificial cultivation and improve some problems such as auto-lysis on commercialization of Coprinus comatus that has been known edible and medicinal mushroom, they were conducted for selection of superior strain, suitable culture methods for mycelial growth and fruiting, and morphological characteristics of fruit body. Strain CM 980301 of Coprinus comatus was selected as a superior strain for artificial cultivation. Wheat grain and rice straw full-grown compost media were most effective for preparation of spawn and artificial cultivation of C. comatus, respectively. Spawn running of Coprinus spp. on the rice straw full-grown compost media required to be 15 days from 24 to $28^{\circ}C$. The casing layer incubation before initiation of fruit body formation, required for 13 days at same temperature for spawn running. And then require $10{\sim}11$ days for initiation and $7{\sim}8$ days for development of fruit body from 20 to $24^{\circ}C$. The fruit body of strain CM 980301 was harvested within a week from initiation of primordium formation. The hardness of pileus and stipe that were harvested in optimal stage showed 102 to 169, and 128 to $182\;g/cm^2$, respectively. Yields of srain CM 980301 from the rice straw full-grown compost media was $37.7kg/3.3m^2$. Weight of individual fruit body was 17.9 g in average.

• The Korean Journal of Mycology
• /
• v.36 no.1
• /
• pp.58-62
• /
• 2008

'Miso', a new variety of oyster mushroom was developed for the bottle culture at the Chungnam Agricultural Research & Extension Services. Its mycelium grew rapidly with $8.4{\sim}8.6\;mm/day $ at $25{\sim}28^{\circ}C$ on PDA medium. The optimum pH of the mycelial growth was pH 5.0. It took 24 days for the primitive primordium formation after inoculation on pine sawdust media mixed with 20% wheat bran. Fruiting body color was white, and the shape of pileus was convex-umbonate. In the bottle culture, the yield was 115.7 g per 850 ml bottle. Stipe length was about 54 mm, the number of stipe per bottle was 18.1, the size of pileus was 28 mm, and gill was crowded. The moisture content of 'Miso' was lower than that of Jangan No.5 and Wonhyeong No.1, and contents of protein, ash and sugar of 'Miso' were higher than those of other varieties, and tannin acid content of 'Miso' was lower than that of others. On the basis of AFLP analysis, the 'Miso' was distinct not only from Wonhyeong No.1, but also from their closest relative, oyster mushrooms.

• Journal of Mushroom
• /
• v.14 no.4
• /
• pp.249-252
• /
• 2016

Timing, place, and the high-tech industry to overcome barriers to the introduct of the so-called "smart agriculture". The core factors of precision agriculture, including temperature, humidity, location information, and real summary information, are all significant for ICT controlling technique. The system is a four-season container allowing mushroom product, humidity, illumination, and factors such as carbon dioxide were regulated to create the optimal environment for mushroom cultivation. Hericium erinaceum (also called Lion's Mane Mushroom, Bearded Tooth Mushroom, or Bearded Tooth Fungus) is an edible and medicinal mushroom in the tooth fungus group. During approximately 40 days of incubation, 1.4 kg of oak sawdust was used as the test medium. The humidity, temperature, and $CO_2$ density were maintained at >85-95%, $18-20^{\circ}C$, and 700-1,000 ppm, respectively, in the container type culture system. The time for primordium formation was observed to be 4-7 days. The amount of fruitbody production was 74-95 g per bag. This technique will be used to establish a new community support program, in conjunction with international movement, domestic sales and exports are also planned.