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Characteristics of Mycelial Growth and Fruit Body Development of White Pleurotus ostreatus 'Miso'

흰색느타리버섯 '미소'의 균사배양 및 자실체 생육 특성

  • Kim, Hong-Kyu (Chungnam Agricultural Research & Extension Services) ;
  • Kim, Yong-Gyun (Chungnam Agricultural Research & Extension Services) ;
  • Lee, Byung-Joo (Chungnam Agricultural Research & Extension Services) ;
  • Lee, Ka-Soon (Chungnam Agricultural Research & Extension Services) ;
  • Yang, Eyu-Seog (Chungnam Agricultural Research & Extension Services) ;
  • Park, Myung-Soo (Department of Applied Biology, Chungnam National University) ;
  • Yoo, Young-Bok (National Institute of Agricultural Science & Technology, RDA) ;
  • Kim, Hong-Gi (Department of Applied Biology, Chungnam National University)
  • 김홍규 (충남농업기술원 생물환경과) ;
  • 김용균 (충남농업기술원 생물환경과) ;
  • 이병주 (충남농업기술원 생물환경과) ;
  • 이가순 (충남농업기술원 생물환경과) ;
  • 양의석 (충남농업기술원 생물환경과) ;
  • 박명수 (충남대학교 응용생물학과) ;
  • 유영복 (농업과학기술원 응용미생물과) ;
  • 김홍기 (충남대학교 응용생물학과)
  • Published : 2008.06.30

Abstract

'Miso', a new variety of oyster mushroom was developed for the bottle culture at the Chungnam Agricultural Research & Extension Services. Its mycelium grew rapidly with $8.4{\sim}8.6\;mm/day $ at $25{\sim}28^{\circ}C$ on PDA medium. The optimum pH of the mycelial growth was pH 5.0. It took 24 days for the primitive primordium formation after inoculation on pine sawdust media mixed with 20% wheat bran. Fruiting body color was white, and the shape of pileus was convex-umbonate. In the bottle culture, the yield was 115.7 g per 850 ml bottle. Stipe length was about 54 mm, the number of stipe per bottle was 18.1, the size of pileus was 28 mm, and gill was crowded. The moisture content of 'Miso' was lower than that of Jangan No.5 and Wonhyeong No.1, and contents of protein, ash and sugar of 'Miso' were higher than those of other varieties, and tannin acid content of 'Miso' was lower than that of others. On the basis of AFLP analysis, the 'Miso' was distinct not only from Wonhyeong No.1, but also from their closest relative, oyster mushrooms.

신품종 느타리버섯 '미소'는 충남농업기술원에서 육성한 품종으로 국립종자원에 품종 등록되었고(품종보호권 등록, 제 1688호, 2006. 11. 17), 제 3호 대한민국 우수품종상을 수상한 품종이다(2007 . 12. 7). '미소'의 균사는 $25{\sim}28^{\circ}C$ 온도의 감자배지에서 배양시 일일 $8.4{\sim}8.6\;mm$로 균사생장이 양호하며, 균사배양에 적합한 배지 pH는 5.0이다. 초발이소요일수는 6일로 원형 1호의 9일에 비해 3일 정도 빨랐다. '미소'의 병당 개체수는 18.1개로 원형 1호의 12.3개 보다 많았고, 대의 직경 및 갓의 크기가 원형 1호에 비해 작은 반면 대의 길이는 길었다. '미소' 갓모양은 반반구형${\sim}$편평형이며, 갓의 색은 미백색이다. '미소'의 병당 개체중은 적었지만 개체수가 많아 원형 1호나 장안 5호에 비해 병당 수량은 115.7g으로 원형 1호(96.4g)보다 20% 높았고, 장안 5호(82.4g)보다 40% 높았다. 버섯의 아린맛을 띄는 주된 물질인 Tannin산 함량이 '미소' 1.54%로 원형 1호 1.72%와 장안5호 1.64%보다 그 함량이 낮아 아린 맛이 적었다. AFLP분석한 결과 '미소'는 원형 1호 및 다른 느타리버섯과 구별되었다.

Keywords

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