• Journal of Ginseng Research
• /
• v.37 no.1
• /
• pp.135-141
• /
• 2013

A rapid, sensitive and selective analytical method was developed and validated for the determination of compound K, a major intestinal bacterial metabolite of ginsenosides in human plasma. Liquid-liquid extraction was used for sample preparation and analysis, followed by liquid chromatography tandem spectrometric analysis and an electrospray-ionization interface. Compound K was analyzed on a Phenomenex Luna C18 column ($100{\times}2.00$ mm, 3 ${\mu}m$) with the mobile phase run isocratically with 10 mM ammonium acetate-methanol-acetonitrile (5:47.5:47.5, v/v/v) at a flow rate of 0.5 mL/min. The method was validated for accuracy (relative error <12.63%), precision (coefficient of variation <9.14%), linearity, and recovery. The assay was linear over the entire range of calibration standards i.e., a concentration range of 1 ng/mL to 1,000 ng/mL ($r^2$ >0.9968). The recoveries of compound K after liquid-liquid extraction at 1, 2, 400, and 800 ng/mL were $106.00{\pm}0.08%$, $103.50{\pm}0.19%$, $111.45{\pm}5.21%$, and $89.62{\pm}34.46%$ for intra-day and $85.40{\pm}0.08%$, $94.50{\pm}0.09%$, $112.50{\pm}5.21%$, and $95.87{\pm}34.46%$ for inter-day, respectively. The lower limit of quantification of the analytical method of compound K was 1 ng/mL in human plasma. The developed method was successfully applied to a pharmacokinetic study of compound K after oral administration in ten of healthy human subjects.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.39 no.6
• /
• pp.110-117
• /
• 2011

In order to study the growth models between the growth of Sedum takevimense and growth rate in soil with three types of mix ratios, this experiment was carried out on April 3rd, 2011. A nonlinearity regression analysis was performed using the Logistic and Gompertz models by SPSS. According to the study of growth models of Sedum takevimense, the process of growth and management methods after over-wintering were explicitly determined. According to the measured values, the growth in the soil of $P_1P_2V_1$ and $P_2P_1V_1$ was better than that of $P_1$. Particularly, the average length of Sedum takevimense in the soil of $P_1P_2V_1$ was about twice as great as that in the $P_1$. The fitness test of the two growth models was: The predicted value and measured value were separately compared and analysed, the average fitting precision $R^2$ of the Logistic models was 0.995, but the average $R^2$ of the Gompertz models was below 0.978, which showed that the Logistic models were better than the Gompertz models. The growth models also showed that the growth time of Sedum takevimense was divided into three: rapid, most rapid and slow. When managed in the rapid and the most rapid time, it will grow better.

• Journal of Bio-Environment Control
• /
• v.23 no.1
• /
• pp.26-30
• /
• 2014

Purpose of this study was a development of a sprayer arm auto control system that could be operated according to distance from pear trees for automation of pest control. Auto control system included two parts, hardware and software. First, controller was made with an MCU and relay switches. Two types of ultra-sonic sensors were installed to measure distance from pear trees: one on/off type that detect up to 3 m, and the other continuous type providing 0~5 V output corresponding to distance of 0~3 m. Second, an auto control algorithm was developed to control. Each spraying arm was controlled according to the sensor-based distance from the pear trees. And it could dodge obstacles to protect itself. Max and min signal values were eliminated, when five sensor signals was collected, and then signals were averaged to reduce sensor's noises. According to results of field experiment, auto control test result was better than non auto control test result. Spraying rates were 69.25% (left line) and 98.09% (right line) under non auto control mode, because pear trees were not planted uniformly. But, auto control test's results were 92.66% (left line) and 94.64% (right line). Spraying rate was increased by maintaining distance from tree.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.35 no.10
• /
• pp.1307-1313
• /
• 2011

The mechanical limited slip differential (LSD) in vehicles is being replaced by the electromagnetic LSD because of its fast response and better active control characteristics. The coil housing made of STS 304 is one of the most important parts in the solenoid assembly of the electromagnetic LSD. High geometrical accuracy is a prerequisite for the manufacture of such coil housings, but precision machining is difficult because of the use of STS 304 thin plate and the variance in machining variables. The aim of this study is to optimize the mean and variance of the shape accuracy in the coil housing by finding a robust solution for the machining process conditions. The mean and standard deviation of the jaw contact pressure, cutting speed, and feed rate are considered to be the major parameters for minimizing the geometrical mean and variance. The response surface model based on the second-order Taylor series is combined together to minimize the mean and variance of the shape accuracy of the coil housing.

• Korean Journal of Pharmacognosy
• /
• v.39 no.3
• /
• pp.199-202
• /
• 2008

A high performance liquid chromatographic (HPLC) method for the simultaneous determination of hesperidin and glycyrrhizin was established for the quality control of traditional herbal medicinal preparation, Pyungwi-san (PWS). Separation and quantification were successfully achieved with a Waters XTerra RP18 column ($5{\mu}m$, 4.6 mm I.D. ${\times}$ 150 mm) by gradient elution of a mixture of acetonitrile and water containing 0.03% phosphoric acid (pH 2.03) at a flow rate of 1.0 ml/min. The diode-array UV/vis detector (DAD) was used for the detection and the wavelength for quantification was set at 230 nm. The presence of hesperidin and glycyrrhizin in this extract was ascertained by retention time, spiking with each authentic standard and UV spectrum. All four compounds showed good linearity $(r^2>0.995)$ in a relatively wide concentration ranges. The R.S.D. for intra-day and inter-day precision was less than 7.0% and the limits of detection (LOD) were less than 60 ng. The mean recovery of each compound was 99.0-105.6% with R.S.D. values less than 4.0%. This method was successfully applied to the determination of contents of hesperidin and glycyrrhizin in three commercial products of PWS. These results suggest that the developed HPLC method is simple, effective and could be readily utilized as a quality control method for commercial PWS products.

• Fire Science and Engineering
• /
• v.34 no.1
• /
• pp.127-134
• /
• 2020

When a pregnant woman experiences cardiac arrest, resuscitation is of the utmost importance. Cardiac arrest in pregnant women differs from cardiac arrest in the general population since both mother and fetus need to be taken into consideration. In the event of cardiac arrest, determining whether to deliver the baby is significant. Cardiopulmonary resuscitation is not always successful, and the survival rate depends on the speed and precision of the procedure. In this study, we focus on the case of a 30-year-old pregnant woman who experienced cardiac arrest and whose family was quick to perceive her condition and call the hospital. A witness performed initial cardiopulmonary resuscitation, while rescue workers performed the advanced procedure. In this case, the patient and baby received proper treatment and left the hospital after six days. It is extremely rare for a pregnant patient to achieve return of spontaneous circulation (ROSC) or receive advanced cardiac life support before reaching the hospital. However, the woman in question in this study achieved ROSC and received both cardiopulmonary resuscitation before reaching the hospital and advanced cardiac life support at the hospital. The specifics of the case are reported in the context of a literature review.

• Korean Journal of Clinical Pharmacy
• /
• v.16 no.1
• /
• pp.23-27
• /
• 2006

Doxorubicin (DXR) is a type of anti-cancer drug called an 'anthracycline glycoside', It works by impairing DNA synthesis, a crucial feature of cell division, and thus is able to target rapidly dividing cells. Doxorubicin is a very serious anti-cancer medication with definite potential to do great harm as well as great good. A liquid chromatography-tandem mass spectroscopy (LC-MS/MS) method was developed to identify and quantify DXR in small-volume biological samples. After the addition of internal standard (IS, $5{\mu}L\;of\;1{\mu}M/ml$ daunorubicin methanol solution) into the serum sample, the drug and IS were extracted by methanol. Following vortex for a 1min and centrifugation at 15,000g for 10 min the organic phase was transferred and evaporated under a vacuum. The residue was reconstituted with $350{\mu}L$ of mobile phase and $10{\mu}L$ was injected into C18 column with mobile phase composed of 0.05M ammonium acetate (0.1 M acetic acid adjusted to pH 3.5) and acetonitrile (40:60, v/v). The flow rate was kept constant at $350{\mu}L/min$. The ions were quantified in the multiple reaction mode (MRM), using positive ions, on a triple quadrupole mass spectrometer. The lower limits of quantification for Doxorubicin in plasma and small tissues were approximately 0.5 ng/mL and 0.5 ng/mL respectively. Intra- and inter-assay accuracy (% of nominal concentration) and precision (% CV) for all analytes were within 15%, respectively.

• Journal of the Institute of Electronics Engineers of Korea SP
• /
• v.44 no.1
• /
• pp.10-16
• /
• 2007

In this paper, we propose a technique for retrieving images using spatial color correlation and texture characteristics based on local fourier transform. In order to retrieve images, two new descriptors are proposed. One is a color descriptor which represents spatial color correlation. The other is a descriptor combining the proposed color descriptor with texture descriptor. Since most of existing color descriptors including color correlogram which represent spatial color correlation considered just color distribution between neighborhood pixels, the structural information of neighborhood pixels is not considered. Therefore, a novel color descriptor which simultaneously represents spatial color distribution and structural information is proposed. The proposed color descriptor represents color distribution of Min-Max color pairs calculating color distance between center pixel and neighborhood pixels in a block with 3x3 size. Also, the structural information which indicates directional difference between minimum color and maximum color is simultaneously considered. Then new color descriptor(min-max color correlation descriptor, MMCCD) containing mean and variance values of each directional difference is generated. While the proposed color descriptor includes by far smaller feature vector over color correlogram, the proposed color descriptor improves 2.5 % ${\sim}$ 13.21% precision rate, compared with color correlogram. In addition, we propose a another descriptor which combines the proposed color descriptor and texture characteristics based on local fourier transform. The combined method reduces size of feature vector as well as shows improved results over existing methods.

• Journal of Environmental Health Sciences
• /
• v.42 no.1
• /
• pp.53-60
• /
• 2016

Objectives: Preservatives are commonly used in pharmaceuticals, cosmetics and other products to extend the expiration date and prevent the growth of microorganisms. Preservatives are generally effective in controlling mold and inhibiting yeast growth, and against a wide range of bacterial attacks as well. They also adversely affect the quality of sperm and cause precocious puberty in children. This study was performed to analyze seven preservatives used in pharmaceuticals and personal care products. Methods: Five kinds of pharmaceuticals and personal care products (PPCPs) were examined for analysis with a high performance liquid chromatography-diode array detector. Each sample was homogenized and the targeted compounds were extracted with methanol. The suspended particulate was removed by syringe filter. Next, the sample was injected into an HPLC system. The separation of the seven preservatives was achieved with a C18 column and gradient mode. The accuracies were between 73% and 120% and precision was lower than 11.58% (RSD). Results: All of the calibration curves showed good linearity with a coefficient of determination ($r^2$) over 0.999. Among the PPCP samples, the detection rate of preservatives was 32.5% for pharmaceuticals, 44.8% for toothpaste, 76.9% for mouthwash, 40.0% for body lotion and 56.0% for wet tissues. The average concentrations of the preservatives in PPCPs were BA 1141.0 mg/kg, MP 709.8 mg/kg, EP 624.9 mg/kg, PP 216.9 mg/kg, BP 167.8 mg/kg, and TCS 538.2 mg/kg. The most frequently detected preservatives in pharmaceuticals and personal care products were BA, MP and PP. The concentrations of preservatives exceeded Korean regulatory standards in 11 samples of medicines, three of mouthwash and two of body lotion. Conclusion: We found that most of the PPCP samples contained various preservatives. It is necessary to identify which preservatives were used and to determine the level of preservatives in PPCPs and to assess the health risk to susceptible populations such as children.

• Journal of Korean Society for Atmospheric Environment
• /
• v.23 no.5
• /
• pp.547-556
• /
• 2007

The release, sampling and analytical methods have been developed and tested for perfluorocarbons (PFCs) atmospheric tracers in order to gain insight into the atmospheric transport and dispersion over the urban conditions of Seoul, Korea. Although PFCs tracer experiments provide unique opportunities to test local and urban scale of transport and dispersion, no previous experiment with PFCs has been conducted in Korea. PMCH and PDCH were chosen as targeted tracers in our study due to their extreme low ambient concentrations and great sensitivities among various PFCs. For PFCs release system, a set of micro-metering pump, electronic balance, vaporizing furnace and high speed blower was constructed for precise and accurate release of tracers. The precision of released rate by this system was estimated to be 1%. Samplings of PFCs were carried out by fabricated portable air samplers with micro pumps and rotameters into glass tubes packed with 150 mg of Carboxen-569. The uncertainty of these sampling system was maintained below 14%. PMCH and PDCH were quantified in GC/ECD with preconditioned injection system to eliminate the interference compounds using traps and subsequent catalytic conversion system prior to column separation. Three intensive field test were undertaken during the springtime of 2002 to 2004 in eastern part of Seoul. Daily background samples were collected to characterize the background levels of PMCH and PDCH prior to their release. The observed background concentrations of PMCH ranged from 3.5 to 10.1 fL/L and varied randomly in location and time in this study. Its mean and standard variation of background concentration ($6.8{\pm}1.9\;fL/L$) are higher than those ($3.2{\sim}5.8\;fL/L$) of other historic tracer studies. Identified uncertainty for background PMCH was $1.7{\sim}2.0\;fL/L$ using this analytical system. Combined relative uncertainty in determining the tracer's concentrations was estimated as 17%. However, its background concentrations and uncertainty in concentration determination were found to be low and stable enough for tracer study.