• Title/Summary/Keyword: Pre-detection

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Development of Total Cholesterol Detection System by Fluorescence Chromatography (형광 크로마토그래피에 의한 콜레스테롤 측정법의 개발)

  • Oh, Sang-Wook
    • Journal of Food Hygiene and Safety
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    • v.24 no.2
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    • pp.148-153
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    • 2009
  • In this study, we have developed a fluorescence chromatographic assay for the quantification of total cholesterol in serum, which is a well-known risk predictor for cardiovascular diseases. The new assay system consists of a chromatographic strip in a cartridge, enzyme buffer containing cholesterol esterase, cholesterol oxidase, horseradish peroxidase, and color developer AEC, and a laser fluorescence scanner. The correlation coefficient (r) between cholesterol concentration and relative fluorescence units was 0.968 in the new assay, showing a reliable linearity through the tested range of cholesterol. Recovery test and comparability with a Hitachi 747 instrument showed 106.5-94% and r = 0.939 (p<0.001), respectively. The new assay system for cholesterol was developed as a pre-POCT platform conducted in clinics since it is fast (8 min) and uses a small volume of sample ($5\;{\mu}l$), and it may be applied for on-site diagnostics to replace expensive automated biochemical analyzer.

The Development of Landslide Predictive System using Measurement Information based on u-IT (u-IT기반 계측정보를 이용한 급경사지붕괴 예측 시스템 개발)

  • Cheon, Dong-Jin;Park, Young-Jik;Lee, Seung-Ho;Kim, Jeong-Seop;Jung, Do-Young
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.10
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    • pp.5115-5122
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    • 2013
  • This paper has studied about the development and application of landslide collapse prediction real-time monitoring system based on USN to detect and measure the collapse of landslide. The rainfall measuring sensor, gap water pressure sensor, indicator displacement measuring sensor, index inclination sensor, water content sensor and image analysis sensor are selected and these are applied on the test bed. Each sensor's operation and performance for reliability verification is tested by the instrument which is installed in the field. As the result, u-IT based real-time landslide monitoring system which is developed by this research for landslide collapse detection could minimize life and property damages because it makes advance evacuation with collapse risk pre-estimate through real-time monitoring on roadside cut and bedrock slopes. This system is based on the results of this study demonstrate the effect escarpment plan are spread throughout.

Optimum Quality Control of Seismic Data of Kunsan Basin in Offshore Korea (국내대륙붕 군산분지에 대한 탄성파 전산처리의 최적 매개 변수 결정)

  • Kim, Kun-Deuk
    • Geophysics and Geophysical Exploration
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    • v.1 no.3
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    • pp.161-169
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    • 1998
  • The Kunsan basin is a pull-apart basin which was formed during Tertiary. The pre-Tertiary section consists of various rock types, such as meta-sediments, igneous rocks, carbonates, clastics, and volcanics. Tertiary sections are the main targets for the petroleum exploration. In order to determine the optimum processing parameters of the basin, about 12 kinds of test processings were performed. The first main steps for the quality control is to determine the noisy or bad traces by examining the near trace section and shot gathers. The true amplitude recovery was applied to account for the amplitude losses due to spherical divergence and inelastic attenuation. Source designature and predictive deconvolution test were conducted to determine the optimum wavelet parameters and to remove the multiples. Velocity analysis was performed at 1km intervals. The optimum mute function was picked by locating the range of offsets which gives the best stacking response for any particular reflections. Post-stack deconvolution was tested to see if the quality of stacked data improved. The stacked data was migrated using a finite difference algorithm. The migration velocity was obtained from the stacking velocities using the time varying percentages. The AGC sections were provided for the structural interpretation. The RAP sections were used for DHI analysis and for the detection of volcanics.

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A Solid-Contact Indium(III) Sensor based on a Thiosulfinate Ionophore Derived from Omeprazole

  • Abbas, Mohammad Nooredeen;Amer, Hend Samy
    • Bulletin of the Korean Chemical Society
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    • v.34 no.4
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    • pp.1153-1159
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    • 2013
  • A novel solid-contact indium(III)-selective sensor based on bis-(1H-benzimidazole-5-methoxy-2-[(4-methoxy-3, 5-dimethyl-1-pyridinyl) 2-methyl]) thiosulfinate, known as an omeprazole dimer (OD) and a neutral ionophore, was constructed, and its performance characteristics were evaluated. The sensor was prepared by applying a membrane cocktail containing the ionophore to a graphite rod pre-coated with polyethylene dioxythiophene (PEDOT) conducting polymer as the ion-to-electron transducer. The membrane contained 3.6% OD, 2.3% oleic acid (OA) and 62% dioctyl phthalate (DOP) as the solvent mediator in PVC and produced a good potentiometric response to indium(III) ions with a Nernstian slope of 19.09 mV/decade. The constructed sensor possessed a linear concentration range from $3{\times}10^{-7}$ to $1{\times}10^{-2}$ M and a lower detection limit (LDL) of $1{\times}10^{-7}$ M indium(III) over a pH range of 4.0-7.0. It also displayed a fast response time and good selectivity for indium(III) over several other ions. The sensor can be used for longer than three months without any considerable divergence in potential. The sensor was utilized for direct and flow injection potentiometric (FIP) determination of indium(III) in alloys. The parameters that control the flow injection method were optimized. Indium(III) was quantitatively recovered, and the results agreed with those obtained using atomic absorption spectrophotometry, as confirmed by the f and t values. The sensor was also utilized as an indicator electrode for the potentiometric titration of fluoride in the presence of chloride, bromide, iodide and thiocyanate ions using indium(III) nitrate as the titrant.

Analytical Method for Triazine Herbicide Cyanazine Residues in Major Medicinal Crops (주요 약용작물에 대한 Cyanazine 제초제의 잔류 분석법)

  • Hwang, Young Sun;Lim, Jung Dae;Choung, Myoung Gun
    • Korean Journal of Medicinal Crop Science
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    • v.24 no.3
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    • pp.237-245
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    • 2016
  • Background: Cyanazine is used as a pre-emergent herbicide once during the growing season to control weeds of many upland crops worldwide. This study aimed to establish a method to determined cyanazine residue levels in major medicinal crops by using high performance liquid chromatography-UV detection/mass spectometry (HPLC-UVD/MS). Methods and Results: Cyanazine residue was extracted with acetone from the raw products of four representative medicinal plants - Scutellaria baicalensis, Paeonia lactiflora, Platycodon grandiflorum and Angelica gigas. The extract was diluted with a large volume of saline water and directly partitioned into dichloromethane to remove polar co-extractives in the aqueous phase. It was then purifined using optimized Florisil column chromatography. HPLC analysis conducted using an octadecylsilyl column allowed the successful separation of cyanazine from co-extractives of the samples, and the amount was sensitively quantified by ultraviolet absorption at 225 nm with no interference. The accuracy and precision of the proposed method were validated by conducting recovery experiments on each medicinal crop sample fortified with cyanazine at two concentration levels per crop in triplicate. Conclusions: The mean recoveries ranged from 91.2% to 105.3% for the four representative medicinal crops. The coefficients of variation were less than 10%, irrespective of the sample types and fortification levels. The limit of quantification of cyanazine was 0.02 mg/kg as verified by the recovery experiment. A confirmatory method was performed by liquid chromatography/MS using selected-ion monitoring technique to clearly identify the suspected residue.

Reasoning Occluded Objects in Indoor Environment Using Bayesian Network for Robot Effective Service (로봇의 효과적인 서비스를 위해 베이지안 네트워크 기반의 실내 환경의 가려진 물체 추론)

  • Song Youn-Suk;Cho Sung-Bae
    • Journal of KIISE:Computing Practices and Letters
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    • v.12 no.1
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    • pp.56-65
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    • 2006
  • Recently the study on service robots has been proliferated in many fields, and there are active developments for indoor services such as supporting for elderly people. It is important for robot to recognize objects and situations appropriately for effective and accurate service. Conventional object recognition methods have been based on the pre-defined geometric models, but they have limitations in indoor environments with uncertain situation such as the target objects are occluded by other ones. In this paper we propose a Bayesian network model to reason the probability of target objects for effective detection. We model the relationships between objects by activities, which are applied to non-static environments more flexibly. Overall structure is constructed by combining common-cause structures which are the units making relationship between objects, and it makes design process more efficient. We test the performance of two Bayesian networks for verifying the proposed Bayesian network model through experiments, resulting in accuracy of $86.5\%$ and $89.6\%$ respectively.

Detection of E. coli O157:H7 and Listeria monocytogenes, and Appraisal for Microbiological Qualities in the Commercial Frozen Yogurt Products in Korea (국내 시판용 Frozen Yogurt의 병원성 미생물 검출 및 미생물학적 품질 평가에 관한 연구)

  • 윤성식
    • Food Science of Animal Resources
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    • v.18 no.1
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    • pp.63-74
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    • 1998
  • Recently the high outbreaks of intestinal disease caused by the consumption of frozen dairy foods containing pathogenic bacteria has generated considerable interest in the causative agent such as Listeria monocytogenes and E. coli O157:H7. This study was carried out to detect the above pathogens and compare the microbiological qualities of three commercial forzen yogurt products. The main results obtained were as follows. L. monocytogenes coliforms and E. coli O157:H7 were not detected in the total of seven frozen yogurt samples. For microbiological qualities the viable lactic counts of products manufactured by FA company were about 2.9$\times$108 -1.6$\times$109cfu/ml 1.7$\times$106 cfu/ml for FB's and 1.2$\times$106 cfu/ml for FC's The PH values of FA's FB's and FC's products was in the range of pH 4.1~5.3 and the values of FA's were 4.1~4.6 compared by the pH 5.2~5.3 of FB's and FC's products. During refrigeration of the test samples the survival rates of L. monocytogenes spiked into thawed frozen yogurt sample(FA's FB,s and FC's) were 0.55% 15.61% and 16.89% respectively. On the other hand E. coli O157:H7 and L. monocytogenes were 12.4% and 25.0% for FA's 10.8% and 20.8% for FB's and 10.26% and 22.7% for FC's under 37$^{\circ}C$ storage, As the results described above each frozen yogurt products were different in microbiological qualities. The survival rates of pathogens spiked into the samples increased with the pH of the products. This indicates that the pH or any other factors pre-sumable supressed the growth of E. coli O157:H7 and L. monocytogenes in frozen yogurt products.

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IMMUNOHISTOCHEMICAL DETECTION OF GROWTH FACTORS AND EXTRACELLULAR MATRIX PROTEINS IN THE DEGENERATING TISSUES OF PRE-AND POSTNATAL HUMAN CLEFT LIP AND PALATE (태생 및 생후 구순.구개열에 나타나는 조직변성에 대한 성장인자와 세포외 기질 단백의 면역조직화학적 연구)

  • Min, Bong-Gi;Lee, Suk-Keun;Park, Young-Wook
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.28 no.6
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    • pp.421-433
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    • 2002
  • In order to elucidate the pathogenesis of cleft lip and palate, first of all, it is necessary to understand the developmental mechanisms of growth factors and extracellular matrix proteins in the tissues of cleft lip and palate. We have performed immunohistochemical studies on human cleft lip and palate tissues to elucidate the pathogenetic implications of cleft lip and palate. 16 specimens from postnatal human cleft lip and palate subjects and 17 specimens from autopsy of prenatal human cleft lip and palate were fixed in 10% buffered formalin, embedded in paraffin. The sections were routinely stained by hematoxylin and eosin, also stained by PAS, and followed by immunohistochemical stainings using the antiseras of growth factors and extracellular matrix proteins such as PCNA, S-100, c-erb-B2, MMP-3, MMP-10, HSP-70, transglutaninase-C, E-cadherin, VEGF, vWF. Both the prenatal and postnatal specimens of cleft lip and palate showed dysplastic proliferation of the basal cell layer, increased infiltration of melanocytes into mucosal epithelium, sebaceous gland hyperplasia ingrowing into the muscular tissue of lip and palate, and fatty infiltration into the submucosal deep connective tissue. The strong reactions of MMP-3 and HSP-70 were detected in the tissues of cleft lip and palate, especially increased in degenerating muscle bundles, while the immunostainings of PCNA and c-erb-B2 were weakly positive in the tissues of cleft lip and palate. These data suggest that the retrogressive tissue degeneration around the cleft areas persistently exist during the prenatal and postnatal period after cleft formation, and the sebaceous gland hyperplasia and fatty infiltration with the intense expression of MMP-3 and HSP-70 is closely related to the muscular degeneration around the cleft area.

DIAGNOSIS OF MESIODENS BY $SCANORA^{(R)}$ ($SCANORA^{(R)}$를 이용한 Mesiodens의 진단)

  • Jeon, Hyung-Joon;Kim, Jong-Soo;Kwon, Soon-Won
    • Journal of the korean academy of Pediatric Dentistry
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    • v.27 no.4
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    • pp.490-493
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    • 2000
  • Mesiodens are supernumerary teeth in the region of the maxillary central incisors, a condition which can lead to disorders of the dentition. Their presence may lead to disorders, such as delay in eruption of permanent teeth, development of dentigerous cysts, resorption of adjacent roots and eruption of a supernumerary tooth into nasal cavity. The optimal time for surgical intervention is controversial. Early diagnosis is important so as to enable good prognosis. Diagnosis primarily depends on x-ray films. Panorama film, occlusal film, periapical film have been used for detection of mesiodens. But, all of them have disadvantages. $SCANORA^{(R)}$ is a multimodal radiology system which utilizes the principles of narrow beam radiology and spiral tomography. Pre-programmed imaging procedure are provided for many dental situations An optional personal computer can be connected into the unit to help design the examination. We report two cases diagnosed by $SCANORA^{(R)}$. When compared with tube shift technique, it is simple and exact method of detecting mesiodens.

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A Small-and-medium-sized Hospital's Crisis Management during 2015 MERS Outbreak: A Case of G Hospital (중소병원의 2015 MERS 위기 대응: G병원의 사례)

  • Son, Heejung;Kim, Kwang-Jum
    • Korea Journal of Hospital Management
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    • v.22 no.3
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    • pp.144-156
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    • 2017
  • Purpose: Crisis is inevitable to every organization and therefore, successful crisis management is critical to the organizations' survival and prosperity. With the understanding, this study aims to draw propositions for successful crisis management of hospitals when facing infectious disease outbreak. For the purpose, a case of a small and medium sized hospital's experience of crisis management during 2015 Middle East Respiratory Syndrome outbreak was analyzed. Methodology/Approach: The detailed internal circumstances and experiences of the hospital during the MERS outbreak were identified by in-depth interview as well as the extensive material review, and analyzed under the view of the theories of accident, error, and crisis in relation of organization management Findings: Overall, nine propositions are drawn by the phase of crisis. In pre-crisis phase, for example, 'the hospital preparedness has positive influence on the effective responding to the crisis'. In detection phase, 'the mindfulness of the hospital organizations' as well as the individuals' has positive influence on detecting the crisis signals'. In crisis phase, for example, 'improvising naturally occurs in crisis by the unknown disease, therefore, a component site supervisor coordinating such improvision is important'. Lastly, in post-crisis phase, 'successful crisis responding experience facilitates the positive hospital culture'. Practical implication: From the experience of a small and medium size hospital, it is suggested that proactive system approach oriented by safety is beneficial for effective crisis management.