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Development of Total Cholesterol Detection System by Fluorescence Chromatography  

Oh, Sang-Wook (Division of Science Education, Institute of Fusion Science, Institute of Science Education, Chonbuk National University)
Publication Information
Journal of Food Hygiene and Safety / v.24, no.2, 2009 , pp. 148-153 More about this Journal
Abstract
In this study, we have developed a fluorescence chromatographic assay for the quantification of total cholesterol in serum, which is a well-known risk predictor for cardiovascular diseases. The new assay system consists of a chromatographic strip in a cartridge, enzyme buffer containing cholesterol esterase, cholesterol oxidase, horseradish peroxidase, and color developer AEC, and a laser fluorescence scanner. The correlation coefficient (r) between cholesterol concentration and relative fluorescence units was 0.968 in the new assay, showing a reliable linearity through the tested range of cholesterol. Recovery test and comparability with a Hitachi 747 instrument showed 106.5-94% and r = 0.939 (p<0.001), respectively. The new assay system for cholesterol was developed as a pre-POCT platform conducted in clinics since it is fast (8 min) and uses a small volume of sample ($5\;{\mu}l$), and it may be applied for on-site diagnostics to replace expensive automated biochemical analyzer.
Keywords
Total cholesterol; Fluorescence; Chromatographic assay; Laser fluorescence scanner;
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