한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제24권3호
  • /
  • Pages.237-245
  • /
  • 2016
  • /
  • 1225-9306(pISSN)
  • /
  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
권호 표지
DOI QR코드

DOI QR Code

주요 약용작물에 대한 Cyanazine 제초제의 잔류 분석법

Analytical Method for Triazine Herbicide Cyanazine Residues in Major Medicinal Crops

  • 황영선 (텍사스주립대 알링턴캠퍼스 생물학과) ;
  • 임정대 (강원대학교 생약자원개발학과) ;
  • 정명근 (강원대학교 생약자원개발학과)
  • Hwang, Young Sun (Department of Biology, University of Texas-Arlington) ;
  • Lim, Jung Dae (Department of Herbal Medicine Resources, Kangwon National University) ;
  • Choung, Myoung Gun (Department of Herbal Medicine Resources, Kangwon National University)
  • 투고 : 2016.04.21
  • 심사 : 2016.06.07
  • 발행 : 2016.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Background: Cyanazine is used as a pre-emergent herbicide once during the growing season to control weeds of many upland crops worldwide. This study aimed to establish a method to determined cyanazine residue levels in major medicinal crops by using high performance liquid chromatography-UV detection/mass spectometry (HPLC-UVD/MS). Methods and Results: Cyanazine residue was extracted with acetone from the raw products of four representative medicinal plants - Scutellaria baicalensis, Paeonia lactiflora, Platycodon grandiflorum and Angelica gigas. The extract was diluted with a large volume of saline water and directly partitioned into dichloromethane to remove polar co-extractives in the aqueous phase. It was then purifined using optimized Florisil column chromatography. HPLC analysis conducted using an octadecylsilyl column allowed the successful separation of cyanazine from co-extractives of the samples, and the amount was sensitively quantified by ultraviolet absorption at 225 nm with no interference. The accuracy and precision of the proposed method were validated by conducting recovery experiments on each medicinal crop sample fortified with cyanazine at two concentration levels per crop in triplicate. Conclusions: The mean recoveries ranged from 91.2% to 105.3% for the four representative medicinal crops. The coefficients of variation were less than 10%, irrespective of the sample types and fortification levels. The limit of quantification of cyanazine was 0.02 mg/kg as verified by the recovery experiment. A confirmatory method was performed by liquid chromatography/MS using selected-ion monitoring technique to clearly identify the suspected residue.

키워드

참고문헌

  1. Association of Analytical Chemists(AOAC). (2000). Pesticide and industrial chemical residues, In Official method of analysis (17th ed.). Association of Analytical Chemists. Arlington. VA, USA. p.1-88.
  2. Ardrey RE. (2003). Liquid chromatography-mass spectrometry:An introduction. John Wiley and Sons. Chichester, England. p.98-122.
  3. Choi YH, Park SK, Cho TH, Ha KT, Seung HJ, Kim SJ, Lee KA, Jang JI, Jo HB and Choi BH. (2008). Pesticide residues in medicinal herbs. Report of Seoul Institute of Health Environment. 44:70-85.
  4. Chung IM, Park IM and Kim SH. (2011). Development and comparison of analytical methods for measuring simazine herbicide using gas chromatography/ion trap, gas chromatography/mass selective detector, and high performance liquid chromatography/triple quadrupole mass spectrometer. Journal of the Korean Society for Applied Biological Chemistry. 54:744-749. https://doi.org/10.1007/BF03253154
  5. Codex Alimentarius Commission(CAC). (2003). Guidelines on good laboratory practice in residue analysis: CAC/GL 40-1993, Rev.1-2003. Codex Alimentarius Commission. Rome, Italy. p.2-36.
  6. Fong WG, Moye HA, Seiber JN and Toth JP. (1999). Pesticide residues in food: Methods, technologies and regulations. John Wiley and Sons. Chichester, England. p.3-4, 40-44.
  7. Hogendoorn EA and Goewie CE. (1989). Residue analysis of the herbicides cyanazine and bentazone in sugar maize and surface water using high-performance liquid chromatography and an on-line clean-up column-switching procedure. Journal of Chromatography A. 475:432-441. https://doi.org/10.1016/S0021-9673(01)89700-4
  8. Hwang JI, Jeon YH, Kim HY, Kim JH, Lee YJ, Park JY, Kim DH and Kim JE. (2011). Application of macroporous diatomaceous earth column for residue analysis of insecticide endosulfan in herbal medicines. Korean Jouranal of Environmental Agriculture. 30:60-67. https://doi.org/10.5338/KJEA.2011.30.1.60
  9. Infante-Rivard C and Weichenthal S. (2007). Pesticides and childhood cancer: An update of Zahm and Ward's 1998 review. Journal of Toxicology and Environmental Health, Part B. 10:81-99. https://doi.org/10.1080/10937400601034589
  10. Korea Customs Service(KCS). (2016). Notices of management of distribution track record after customs clearance about import items. Korea Customs Service. Daejeon, Korea. http://www.customs.go.kr/kcshome/law/rule/RuleUserDetail.do?layoutMenuNo=20215&admRul=2&admRulSeq=4251(Citedby 2016 May 19).
  11. Korea Food and Drug Administration(KFDA). (2011). Acceptance criteria and test methods about residual and pollutant in herbal medicine. Korea Food and Drug Administration. Cheongju, Korea. https://lawwizice.wordpress.com/tag/%EC%A0%9C2011-27%ED%98%B8/(cited by 2016 Jan 13).
  12. Korea Food and Drug Administration(KFDA). (2012). Pesticide analytical residues manual in food code. Korea Food and Drug Administration. Cheongju, Korea. p.3-60.
  13. Kwon CH, Chang MI, Im MH, Choi H, Jung DI, Lee SC, Yu JY, Lee YD, Lee JO and Hong MK. (2008). Determination of mandipropamid residues in agricultural commodities using highperformance liquid chromatography with mass spectrometry. Analytical Science and Technology. 21:518-525.
  14. Lee AR, Jang S, Kim TH, Lee AY, Choi G and Kim HK. (2013). Monitoring of residual sulfur dioxide in herbal medicines distributed at domestic. Korean Journal of Medicinal Crop Science. 21:124-129. https://doi.org/10.7783/KJMCS.2013.21.2.124
  15. Lee JH, Park HW, Keum YS, Kwon CH, Lee YD and Kim JH. (2008). Dissipation pattern of boscalid in cucumber under greenhouse condition. The Korean Journal of Pesticide Science. 12:67-73.
  16. Lee JH, Shin KS, Jeon YH, Kim HY, Hwang JI, Lee BH, Kang IH, Kang SJ, Kim TH and Kim JE. (2010a). Suggestion for establishment of temporary MRLs and safe use guideline of the organophosphorus insecticides in Jinpi. Korean Jouranal of Environmental Agriculture. 29:66-71. https://doi.org/10.5338/KJEA.2010.29.1.066
  17. Lee SJ, Hwang YS, Kim YH, Nam MY, Hong SB, Yun WK, Kwon CH, Do JA, Im MH, Lee YD and Choung MG. (2010b). Determination of fomesafen residue in agricultural commodities using HPLC-UVD/MS. The Korean Journal of Pesticide Science. 14:95-103.
  18. Lee SJ, Kim YH, Song LS, Hwang YS, Lim JD, Sohn EH, Im MH, Do JA, Oh JH, Kwon KS, Lee JK, Lee YD and Choung MG. (2011). Development of analytical method for fenoxycarb, pyriproxyfen and methoprene residues in agricultural commodities using HPLC-UVD/MS. The Korean Journal of Pesticide Science. 15:254-268.
  19. Lynch SM, Rusiecki JA, Blair A, Dosemeci M, Lubin J, Sandler D, Hoppin JA, Lynch CF and Alavanja MC. (2006). Cancer incidence among pesticide applicators exposed to cyanazine in the agricultural health study. Environmental Health Perspectives. 114:1248-1252. https://doi.org/10.1289/ehp.8997
  20. McLafferty FW and Turecek F. (1993). Interpretation of mass spectra. University Science Books. Sausalito. CA, USA. p.19-50.
  21. Miller JM. (2005). Chromatography: Concepts and contrasts(2nd ed.). John Wiley and Sons. Chichester, England. p.286-287.
  22. Ministry of Food and Drug Safety(MFDS). (2012). Korea food code. Ministry of Food and Drug Safety. Cheongju, Korea. http://fse.foodnara.go.kr/residue/mobile/menu_01_03.jsp?idx=10265(cited by 2015 Oct 9).
  23. Ministry of Health New Zealand(MHNZ). (2007). The investigation and surveillance of agrichemical spraydrift incidents: Guidelines for public health units(revised edition). Ministry of Health New Zealand. Wellington, New Zealand. p.84.
  24. National Health and Family Planning Commission(NHFPC). 2014). Maximum residue limits for pesticides in food. National Health and Family Planning Commission. Beijing, China. p.130.
  25. Snchez-Rasero F and Dios GC. (1988). Liquid chromatographic method for the determination of cyanazine in the presence of some normal soil constituents. Journal of Chromatography A. 447:426-431. https://doi.org/10.1016/0021-9673(88)90056-8
  26. Seo JS, Son SG, Kim KS, Seo SM and Kim DH. (1994). Chemical control of weed for Angelica gigas Nakai. Korean Journal of Medicinal Crop Science. 2:187-192.
  27. Shaner DL, Jason Krutz LJ, Brien Henry WB, Hanson BD, Poteet MD and Rainbolt CR. (2010). Sugarcane soils exhibit enhanced atrazine degradation and cross adaptation to other striazines. Journal American Society of Sugar Cane Technologists. 30:1-10.
  28. World Health Organization(WHO). (2003). Cyanazine in drinkingwater, background document for development of WHO guidelines for drinking-water quality. Geneva, Switzerland. p.1.
  29. Zhang G and Pan J. (2011). Simultaneous spectrophotometric determination of atrazine and cyanazine by chemometric methods. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 78:238-242. https://doi.org/10.1016/j.saa.2010.09.028

피인용 문헌

  1. Development of Individual Residue Analysis Method for Cyanazine in Agricultural Commodities as an Unregistered Herbicide in Korea vol.30, pp.4, 2018, https://doi.org/10.12719/KSIA.2018.30.4.339