• Reproductive and Developmental Biology
• /
• v.31 no.2
• /
• pp.109-113
• /
• 2007

This study was conducted to evaluate the effect of cytochalasin B (CB) treatment in the activation medium on the development of somatic cell nuclear transfer (SCNT) rat embryos. Fetal fibroblast cells were isolated from a Day 14.5 fetus, and the oocytes for recipient cytoplasm were recovered from 4-week old Sprague Dawley rats. After enucleation and nuclear injection, the reconstructed oocytes were immediately exposed to activation medium consisting of 10 mM $SrCl_2$ with or without CB for 4 hr, and formation of pseudo-pronucleus (PPN) was checked at 18 hr after activation. Then, they were transferred into day 1 pseudopregnant recipients (Hooded Wistar) or cultured for 5 days to check their developmental competence in vivo or in vitro. The number of PPN was not affected by CB treatment during the activation. However, CB treatment supported pre-implantation development of rat SCNT embryos. Embryos generated by the procedures of SCNT were also capable of implanting, with 1 implantation scar found from a recipient following the transfer of 87 SCNT embryos to four foster mothers. The result of the present study shows that rat SCNT embryo can develop to post-implantation stage following treatment with CB.

• The Korean Journal of Emergency Medical Services
• /
• v.16 no.2
• /
• pp.53-66
• /
• 2012

Purpose : Emergency medical services in China are increase in demand by people and under the greater pressure than ever before. So it is, necessary to advance the pre-hospital system in order to promote the development of emergency medical services. Methods : This is based on China-related articles, books, journals, reports, statistical data and other literature. Results : First, pre-hospital emergency medical care with the introduction of specialist training program should be established. Second, to strengthen pre-hospital emergency services and to develop the EMS guidelines. Third, the "120" reporting systems unification and awareness activation. Fourth, the preparation of the EMS facilities equipment system. Fifth, the rapid transport system establishment to the selected medical institutions. Conclusion : It is necessary to strengthen the emergency medical personnel at the scene, rapid transport, rapid patient triage and to improve the survival rate of the patients.

• BMB Reports
• /
• v.50 no.10
• /
• pp.522-527
• /
• 2017

A large number of transcriptional activation domains (TADs) are intrinsically unstructured, meaning they are devoid of a three-dimensional structure. The fact that these TADs are transcriptionally active without forming a 3-D structure raises the question of what features in these domains enable them to function. One of two TADs in human glucocorticoid receptor (hGR) is located at its N-terminus and is responsible for ~70% of the transcriptional activity of hGR. This 58-residue intrinsically-disordered TAD, named tau1c in an earlier study, was shown to form three helices under trifluoroethanol, which might be important for its activity. We carried out heteronuclear multi-dimensional NMR experiments on hGR tau1c in a more physiological aqueous buffer solution and found that it forms three helices that are ~30% pre-populated. Since pre-populated helices in several TADs were shown to be key elements for transcriptional activity, the three pre-formed helices in hGR tau1c delineated in this study should be critical determinants of the transcriptional activity of hGR. The presence of pre-structured helices in hGR tau1c strongly suggests that the existence of pre-structured motifs in target-unbound TADs is a very broad phenomenon.

• Animal cells and systems
• /
• v.11 no.2
• /
• pp.129-134
• /
• 2007

The initiation of eukaryotic DNA replication requires assembly of the pre-replicative complex (Pre-RC) through the concerted action of Orc, Cdc6, Cdt1 and Mcm2-7 complex during G1 phase. The pre-RC assembly licenses individual replication origins for the initiation of DNA replication and sufficient number of the pre-RC is essential for proper progression of S phase. However, it is not well known how cells recognize the completion of the pre-RC assembly before G1-S transition. In order to understand the cellular responses to the defects in pre-RC assembly, we depleted the known components of pre-RC proteins using the small interference RNAs in HeLa cells. Although the defects of pre-RC assembly by the depletion of the pre-RC proteins such as Orc2, Cdt1, Mcm2 & Mcm10 did not elicit the activation of Chk1- or Chk2-dependent checkpoint pathways, these cells still showed significant decrease in the cellular level of Cdc25A proteins. These results suggests that a novel checkpoint pathway exist in HeLa cells, which is not dependent upon Chk1 or Chk2 proteins and play essential roles in the cellular responses to the defects in the pre-RC assembly. Also, among those four proteins tested in this study, the depletion of Mcm10 and Cdt1 proteins significantly increased the apoptotic cell death in HeLa cells, suggesting that these proteins not only play roles in the pre-RC assembly, but also are involved in the checkpoint responses to the defects in the pre-RC assembly.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.24 no.5
• /
• pp.872-876
• /
• 2010

The present study found that EA pre-treatment effectively attenuated both spinal ERK activation and hyperalgesia against carrageenan-induced inflammation, whereas EA co-treatment with carrageenan injection unexpectedly elevated ERK activation in a synergistic manner and virtually had no analgesic effect. Therefore, we have concluded that the molecular mechanism of EA anagesia may be related to the inhibition of spinal ERK activation. Further experiments are required to find the intermediate candidates which transmits the pain-alleviating signals on the way of inhibiting ERK activation by EA.

• Journal of the Korean Society of Physical Medicine
• /
• v.3 no.3
• /
• pp.151-160
• /
• 2008

Purpose : The purpose of this study is to know the muscle activation after sub-talar joint mobilition and active exercise, and the low leg muscle activity through the well-balanced interaction of ankle joint around muscle. Methods : For this study 61 experimental subjects are divided into 24 people of supination foot group, pronation foot group 17 people of, 20 people of control group through navicular drop test. Surface EMG was used in order to measure the muscle activities. following is the result of the data analysis about each experiment that has been carried on a week, 2 weeks, 3 weeks, 4 weeks before, and even comparing with pre-experimental state. Results : In electromyogram study, the higher muscle activation there was before the experiment, the more muscle activation increase there was after the experiment in Tibialis Anterior, Peroneus Longus, Peroneus Brevis.(p<.05). Conclusion : This study shows the balanced activation of foot and ankle-around muscle. It shows that foot shape affects the balanced activation recovery of lower leg muscles.

• Journal of the Korea Institute of Military Science and Technology
• /
• v.15 no.1
• /
• pp.81-85
• /
• 2012

This work is related to study the thermal decomposition of 1,3,5-trinitro-1,3,5-triazacylohexane(RDX) by differential scanning calorimeter and thermo-gravimetry with Kissinger's & Iso-conversional method under nonisothermal conditions, with heating rate from 2 to $8^{\circ}C$/min or given heating rate. We calculated and compared activation energy with these two methods. Iso-conversional method is better than Kissinger's method to study decomposition mechanism. We also investigated activation energy and frequency factor by Kissinger's & Iso-conversional method with the influence of particle size. In case of single crystal, Cl-3(large crystal) has better thermal stability than Cl-5(small crystal). The activation energy increased according to the size of the particle size.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.28 no.2
• /
• pp.371-377
• /
• 1999

In order to evaluate the antimicrobial function of natural herb extracts as antimicrobial agent or packaging material for the preservation of foods and greenhouse produce, the water extract of chopi (Zanthoxylum piperitum DC.) was prepared and its antimicobial activity was determined. In the paper disk test its antimicrobial activity was increased in proportion to its concentraion. The growth of microorganisms was completely inhibited above 500ppm of its concentration. It showed wide spectrum of thermal(40 to 180oC) and pH(4 to 10) stabilities. In the electronic microscopic observation(TEM and SEM) of microbial morphological change it showed to decrease the activation of physiological enzymes and to lose the function of cell membranes. Even in the activation test of galactosidase, it seemed to weaken the osmotic function of cell membranes remarkably in comparison with chloroform and its activation corresponded to 40~50% of toluene. Zanthoxylum piperitum DC. extract seemed to be an excellent antimicrobial for the inhibition of food borne microorganisms as well as the pre servation of greenhouse produces.

• The Journal of Korean Physical Therapy
• /
• v.21 no.1
• /
• pp.81-87
• /
• 2009

Purpose: Human brain was lateralized to dominant or non-dominant hemisphere, and could be reorganized by the processing of the motor learning. We reported four cases which showed the changes of the cortical activation patterns resulting from two weeks of training with the serial reaction time task. Methods: Four right-handed healthy subjects were recruited, who was equally divided to two training conditions (right hand training or left hand training). They were assigned to train the serial reaction time task for two weeks, which should press the corresponding four colored buttons as fast as accurately as possible when visual stimulus was presented. Before and after two weeks of training, reaction time and function magnetic resonance image (fMRI) was acquired during the performance of the same serial reaction time task as the training. Results: The reaction time was significantly decreased in all of subjects after training. Our fMRI result showed that widespread bilateral activation at the pre scanning was shifted toward the focused activation on the contralateral hemisphere with progressive motor learning. However, the bilateral activation was still remained during the performance of the non-dominant hand. Conclusion: These findings showed that the repetitive practice of the serial reaction time task led to increase the movement speed and accuracy, as described by motor learning. Such motor learning induced to change the cortical activation pattern. And, the changed pattern of the cortical activation resulting from motor learning was different each other in accordance with the hand dominance.

• English Language & Literature Teaching
• /
• no.3
• /
• pp.95-122
• /
• 1997

The purpose of this research was to investigate the effects of content and formal schema on reading comprehension. Five hundred fiftynine subjects from high school were assigned to one of the following levels and treatment conditions : (1) Higher level & Schema Activation, (2) Higher level & Non-schema Activation, (3) Lower level & Schema Activation, and (4) Lower level & Non-schema Activation. To evaluate the effects of schema activation. two experiments were conducted : one was related to the content schema and the other to the formal schema. To evaluate the effects of content schema, three different types of tests were conducted : (1) cloze test, (2) guessing the meanings of nonsense words, and (3) immediate recall test. To evaluate the effects of formal schema instruction, four kinds of tests were conducted : (1) sorting the sentences according to the importance, (2) identifying the signal words, (3) immediate recall test, and (4) identifying the specific information. For content schema condition, results indicated that the subjects given the titles or pictures before reading in "Content Schema Activation" treatment had better grades than those of the other treatment in all types of tests. regardless of their levels. Schema activation helped the subjects to increase the cognitive predictability of missing words and to participate in the tasks more actively with risk-taking. And it was also shown that good readers tend to process the words meaningfully, while poor readers tend to process the words phonetically or morphologically. Formal schema activation through teaching the text organization also had a significant influence on three types of tests: sorting the sentences according to the importance, identifying the signal words, and immediate recall test, but not on identifying the specific information. The implications from this study can be briefly noted as follows : (l) In teaching reading, the student's background knowledge should be activated as a pre-reading activity. (2) In reading, it is more important to emphasize the student's schema than the features of the text. (3) Various educational interventions should be introduced, especially for the lower level students. (4) Teaching text structures can be a powerful method for the top-down processing strategy.