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Effect of Cytochalasin B in Activation Medium on the Development of Rat Somatic Cell Nuclear Transfer Embryos  

Roh, Sang-Ho (Craniomaxillofacial Reconstructive Science Major and Dental Research Institute, Seoul National University School of Dentistry)
Publication Information
Reproductive and Developmental Biology / v.31, no.2, 2007 , pp. 109-113 More about this Journal
Abstract
This study was conducted to evaluate the effect of cytochalasin B (CB) treatment in the activation medium on the development of somatic cell nuclear transfer (SCNT) rat embryos. Fetal fibroblast cells were isolated from a Day 14.5 fetus, and the oocytes for recipient cytoplasm were recovered from 4-week old Sprague Dawley rats. After enucleation and nuclear injection, the reconstructed oocytes were immediately exposed to activation medium consisting of 10 mM $SrCl_2$ with or without CB for 4 hr, and formation of pseudo-pronucleus (PPN) was checked at 18 hr after activation. Then, they were transferred into day 1 pseudopregnant recipients (Hooded Wistar) or cultured for 5 days to check their developmental competence in vivo or in vitro. The number of PPN was not affected by CB treatment during the activation. However, CB treatment supported pre-implantation development of rat SCNT embryos. Embryos generated by the procedures of SCNT were also capable of implanting, with 1 implantation scar found from a recipient following the transfer of 87 SCNT embryos to four foster mothers. The result of the present study shows that rat SCNT embryo can develop to post-implantation stage following treatment with CB.
Keywords
Somatic cell nuclear transfer; Oocyte activation; Implantation; Cytochalasin B treatment; Rat;
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