• 조경수
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• 통권123호
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• pp.29-30
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• 2011

• 한국환경과학회지
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• 제23권12호
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• pp.2129-2134
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• 2014

The antioxidant activities and total phenolics of four Rosaceae species Pourthiaea villosa (Thunb.) Decne, Sorbus commixta Hedlund, Sorbaria sorbifolia var. stellipila Maxim and Pyrus pyrifolia (Burm.f.) Nakai were determined. Phenolic content (polyphenol and flavonoid), radical scavenging activities [2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis (3-ethylnezthiazoline-6-sulfoic acid) (ABTS)] and ferrous ion chelating effect were evaluated. Total polyphenol and flavonoid contents were highest in Pourthiaea villosa (Thunb.) Decne and lowest in Pyrus pyrifolia (Burm.f.) Nakai. Phenolic contents of Pourthiaea villosa (Thunb.) Decne was $331.45{\pm}7.78$ and $90.4{\pm}3.5mg{\cdot}g^{-1}$. DPPH and ABTS radical scavenging activities were found to be lowest in Sorbus commixta Hedlund whereas Sorbaria sorbifolia var. stellipila Maxim and Pourthiaea villosa (Thunb.) Decne showed relatively good DPPH and ABTS radical scavenging activities. Ferrous ion chelating effect was highest in Pyrus pyrifolia (Burm.f.) Nakai ($1.05{\pm}0.04mg{\cdot}ml^{-1}$) and lowest in Sorbus commixta Hundlund ($4.22{\pm}0.71mg{\cdot}ml^{-1}$).

• 생약학회지
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• 제44권1호
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• pp.16-21
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• 2013

The fruits of Pourthiaea villosa were extracted with methanol and its extract was fractionated with n-hexane, methylene chloride, ethyl acetate and n-butanol. Repeated column chromatography of silica gel, sephadex LH-20 and HPLC led to the isolation of nine phenolic compounds from ethyl acetate soluble fraction. The chemical structures were elucidated as kaemferol-3-O-${\beta}$-D-glucopyranoside (astragalin) (1), isorhamnetin-3-O-${\beta}$-D-glucopyranoside (2), kaempferol-3-O-${\beta}$-D-xylopyranosyl($1{\rightarrow}2$)-${\alpha}$-L-rhamnopyranoside (3), caffeic acid (4), quercetin-3-O-${\beta}$-D-xylopyranosyl($1{\rightarrow}2$)-a-L-rhamnopyranoside (5), quercetin-3-O-${\beta}$-D-xylopyranosyl($1{\rightarrow}2$)-${\beta}$-D-glucopyranoside (6), quercetin-3-O-${\beta}$-D-xylopyranosyl($1{\rightarrow}2$)-${\beta}$-D-galactopyranoside (7), quercetin-3-O-${\alpha}$-L-rhamnopyranoside (quercitrin) (8), and kaempferol-3-O-${\alpha}$-L-rhamnopyranoside (afzelin) (9) by spectroscopic techniques. These compounds were isolated from this plant for the first time.

• 생약학회지
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• 제47권1호
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• pp.12-17
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• 2016

In this study, the purification of the MeOH extract from the stems and leaves of Pourthiaea villosa var. brunnea using column chromatography furnished a main compound, lyoniside. The structure was elucidated on the basis of $^1H$ and $^{13}C$ NMR spectroscopic data. Quantitative analysis of lyoniside was conducted by HPLC method and the highest content of lyoniside was found in 50% MeOH reflux extraction. To investigate the anti-inflammatory effect of the lyoniside, we measured nitric oxide and pro-inflammatory cytokines such as TNF-${\alpha}$, IL-$1{\beta}$, IL-6 levels in lipopolysaccharide-induced murine macrophage cell line RAW 264.7. As a results, lyoniside decreased the level of nitric oxide and IL-6 in concentration dose dependent manner in RAW 264.7 cells.

• 한국자원식물학회지
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• 제28권1호
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• pp.26-34
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• 2015

국내에 분포하는 윤노리나무의 형태적 특징과 변이를 조사하기 위하여 6곳의 윤노리나무 집단을 대상으로 실험을 수행하였다. 분산분석 수행결과 21가지 정량적 형질 중 18가지 형질에서 집단 간에 유의한 차이를 나타내는 것으로 확인되었다. 주성분 분석 결과, 6가지의 주성분이 전체 분산에 대하여 68.28%의 설명력을 갖는 것으로 확인되었다. 제1주성분은 '엽병의 길이'(0.764), 제2주성분은 '잎의 형태'(0.834), 제3주성분은 '잎의 길이'(0.753), 제4주성분은 '수관의 폭'(0.663), 제5주성분은 '화서의 폭'(0.492), 제6주성분은 '엽저의 모양'의 특성이 높은 기여도를 나타냈다. 군집분석을 수행한 결과 '괴산군 쌍곡계곡' 집단이 이질적인 집단으로 확인되었다. 나머지 집단들은 '남해군 미조리상록수림' 집단과 '함양군 상림' 집단이 가깝게 유집되었으며, '남해군 금산' 집단, '진도 첨찰산' 집단, '제주시 바리메오름' 집단이 하나의 소그룹을 이루었다.

• The Korean Journal of Ecology
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• 제25권2호
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• pp.127-134
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• 2002

본 연구는 1997년 3월부터 1998년 10월에 걸쳐 이루어졌으며, 한반도 서남해 도서의 소나무림을 대상으로 식물사회학적인 방법에 의해 군락의 유형을 분류하였다. 26개의 방형구를 선정하여 결과 본 조사 지역의 소나무림은 자금-소나무군락과 방아풀-소나무군락으로 분류되었고, 각각의 군락은 털머위하위군락, 졸참나무하위군락 및 전형하위군락과 노간주나무하위군락, 윤노리나무하위군락 및 전형하위군락으로 구분되었다.

• 한국식품영양학회지
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• 제28권6호
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• pp.1098-1106
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• 2015

This study aimed to investigate antioxidant activities from 11 forest plants, and determine their total phenolics, flavonoids and proantocyanidins contents. In addition, the antioxidant activities were correlated with antioxidant compounds. Among the samples, Cornus officinalis, Castanea crenata, Lindera erythrocarpa, Carpinus laxiflora and Pourthiaea villosa showed significantly higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) ($IC_{50}=21.12{\sim}28.93{\mu}g/mL$) and 2,2'-azino-bis(3-ethylbenzothia zoline-6-sulfonic acid) (ABTS) ($IC_{50}=28.18{\sim}52.55{\mu}g/mL$) radical scavenging ability with reducing power ($IC_{50}=59.91{\sim}93.64{\mu}g/mL$) than other plants; and C. crenata, L. erythrocarpa and Rubus coreanus showed strong nitric oxide (NO) inhibition activity (${\geq}60%$). In addition, L. erythrocarpa, C. laxiflora and P. villosa showed higher oxygen radical absorbance capacity (ORAC) values (${\geq}1,100{\mu}M$ TE/g sample) than other samples. High total phenolic contents were observed in C. crenata (429.11 mg GAE/g), L. erythrocarpa (437.11 mg GAE/g), C. laxiflora (408.67 mg GAE/g) and P. villosa (404.11 mg GAE/g). The DPPH and ABTS radical scavenging activity with reducing power were significantly correlated with total phenolic contents ($R^2=0.71{\sim}0.79$), but total phenolic contents were not correlated with NO inhibition and ORAC ($R^2=0.35{\sim}0.43$). Therefore, these results suggested that C. officinalis, C. crenata, L. erythrocarpa, C. laxiflora and P. villosa are potential natural antioxidative candidate ingredients.

• 생약학회지
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• 제34권1호통권132호
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• pp.100-108
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• 2003

The regulation of plasma lipid level, particularly LDL cholesterol, represents the focus of current therapy for atherosclerosis. And $Lp-PLA_2$ is able to hydrolyse oxidized phosphatidylcholine within LDL into lyso-PC and oxidized fatty acids. $Lp-PLA_2$ is a potential biomarker of coronary heart disease and plays an important proinflammatory role in the progression of atherosclerosis. We investigated the inhibitory effects of methanol extracts of 224 natural plants on $Lp-PLA_2$ activity. Seven kinds of methanol extracts of tested plants showed above 50% inhibitory effect with the concentration of $100\;{\mu}g/ml$. The concentrated aqueous suspensions of each methanol extract were partitioned with n-hexane, $CHCl_3$, and EtOAc. Among them, EtOAc extracts of Astilbe chinensis var. davidii (root) and Pourthiaea villosa var. brunnea (leaf) significantly inhibited $Lp-PLA_2$ activity at the same concentration.

• 한국환경과학회지
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• 제18권12호
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• pp.1427-1436
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• 2009

The purpose of this study was to explain community structure for actual vegetation and their environment variables in Anmyeon Island. Samples were collected for 106 plots using ZM phytosociology method and coincidence method. Actual vegetation were classified into three vegetations types(forest vegetation type, maritime vegetation type, lake vegetation type) and eleven community units. Pourthiaea villosa community in forest vegetation type was divided into 5 groups such as Carpinus coreana group, Pinus rigida group, Chamaecyparis obtusa group, Castanea crenata group and Typical group. Maritime vegetation type was divided into 3 communities, such as Vitex rotundifolia community, Koelreuteria paniculata community and Suaeda japonica community. V. rotundifolia community was subdivided into 2 groups, Rosa rugosa group and Diodia teres group. K. paniculata community was subdivided into 2 groups, Grewia biloba var. parviflora group and Typical group. Lake vegetation type was divided into 1 community, Nelumbo nucifera community. And it was entirely classified into 11 community units.

• The Korean Journal of Ecology
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• 제25권3호
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• pp.197-204
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• 2002

본 연구는 1997년 3월부터 1998년 10월에 걸쳐 이루어졌으며, 한반도 서남해 도서의 소나무림을 대상으로 식물사회학적인 방법에 의해 군락의 유형을 분류하였다. 26개의 방형구를 선정하여 조사한 결과 본 조사 지역의 소나무림은 자금우-소나무군락과 방아풀-소나무군락으로 분류되었고, 각각의 군락은 털머위하위군락, 졸참나무하위군락 및 전형하위군락과 노간주나무하위군락, 윤노리나무하위군락 및 전형하위군락으로 구분되었다.