• Landscaping Tree
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• s.123
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• pp.29-30
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• 2011

• Journal of Environmental Science International
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• v.23 no.12
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• pp.2129-2134
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• 2014

The antioxidant activities and total phenolics of four Rosaceae species Pourthiaea villosa (Thunb.) Decne, Sorbus commixta Hedlund, Sorbaria sorbifolia var. stellipila Maxim and Pyrus pyrifolia (Burm.f.) Nakai were determined. Phenolic content (polyphenol and flavonoid), radical scavenging activities [2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis (3-ethylnezthiazoline-6-sulfoic acid) (ABTS)] and ferrous ion chelating effect were evaluated. Total polyphenol and flavonoid contents were highest in Pourthiaea villosa (Thunb.) Decne and lowest in Pyrus pyrifolia (Burm.f.) Nakai. Phenolic contents of Pourthiaea villosa (Thunb.) Decne was $331.45{\pm}7.78$ and $90.4{\pm}3.5mg{\cdot}g^{-1}$. DPPH and ABTS radical scavenging activities were found to be lowest in Sorbus commixta Hedlund whereas Sorbaria sorbifolia var. stellipila Maxim and Pourthiaea villosa (Thunb.) Decne showed relatively good DPPH and ABTS radical scavenging activities. Ferrous ion chelating effect was highest in Pyrus pyrifolia (Burm.f.) Nakai ($1.05{\pm}0.04mg{\cdot}ml^{-1}$) and lowest in Sorbus commixta Hundlund ($4.22{\pm}0.71mg{\cdot}ml^{-1}$).

• Korean Journal of Pharmacognosy
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• v.44 no.1
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• pp.16-21
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• 2013

The fruits of Pourthiaea villosa were extracted with methanol and its extract was fractionated with n-hexane, methylene chloride, ethyl acetate and n-butanol. Repeated column chromatography of silica gel, sephadex LH-20 and HPLC led to the isolation of nine phenolic compounds from ethyl acetate soluble fraction. The chemical structures were elucidated as kaemferol-3-O-${\beta}$-D-glucopyranoside (astragalin) (1), isorhamnetin-3-O-${\beta}$-D-glucopyranoside (2), kaempferol-3-O-${\beta}$-D-xylopyranosyl($1{\rightarrow}2$)-${\alpha}$-L-rhamnopyranoside (3), caffeic acid (4), quercetin-3-O-${\beta}$-D-xylopyranosyl($1{\rightarrow}2$)-a-L-rhamnopyranoside (5), quercetin-3-O-${\beta}$-D-xylopyranosyl($1{\rightarrow}2$)-${\beta}$-D-glucopyranoside (6), quercetin-3-O-${\beta}$-D-xylopyranosyl($1{\rightarrow}2$)-${\beta}$-D-galactopyranoside (7), quercetin-3-O-${\alpha}$-L-rhamnopyranoside (quercitrin) (8), and kaempferol-3-O-${\alpha}$-L-rhamnopyranoside (afzelin) (9) by spectroscopic techniques. These compounds were isolated from this plant for the first time.

• Korean Journal of Pharmacognosy
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• v.47 no.1
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• pp.12-17
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• 2016

In this study, the purification of the MeOH extract from the stems and leaves of Pourthiaea villosa var. brunnea using column chromatography furnished a main compound, lyoniside. The structure was elucidated on the basis of $^1H$ and $^{13}C$ NMR spectroscopic data. Quantitative analysis of lyoniside was conducted by HPLC method and the highest content of lyoniside was found in 50% MeOH reflux extraction. To investigate the anti-inflammatory effect of the lyoniside, we measured nitric oxide and pro-inflammatory cytokines such as TNF-${\alpha}$, IL-$1{\beta}$, IL-6 levels in lipopolysaccharide-induced murine macrophage cell line RAW 264.7. As a results, lyoniside decreased the level of nitric oxide and IL-6 in concentration dose dependent manner in RAW 264.7 cells.

• Korean Journal of Plant Resources
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• v.28 no.1
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• pp.26-34
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• 2015

This study was conducted to examine the morphological characteristics and variations of the 6 populations of Pourthiaea. villosa (Thunb.) Decne. var. villosa in Korea. The ANOVA results showed that the populations were significantly different in 18 of the 21 quantitative characteristics that were analyzed. In the results of principal component analysis, 6 principal components (PC) represented 68.28% for the total variations. 'Petiole length' (0.764), 'Leaf shape' (0.834), 'Leaf length' (0.753), 'Crown diameter' (0.663), 'Inflorescence width' (0.492), and 'Leaf base shape' (0.721) showed the highest contribution to PC1, PC2, PC3, PC4, PC5, and PC6, respectively. According to the results of cluster analysis, one cluster comprised only 'Goesan-gun Ssanggok Valley' population. 'Namhae-gun Mijori Evergreen Forest' population was grouped with 'Hamyang-gun Sang Forest' population, which was the nearest subgroup. Further, 'Namhae-gun Geum Mountain' population, 'Jindo-gun Cheomchal Mountain' population, and 'Jeju-si Barimae Parasitic volcano' population were placed in the same cluster.

• The Korean Journal of Ecology
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• v.25 no.2
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• pp.127-134
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• 2002

This study was carried out to analyze Pinus densiflora forest in islets of southwestern coast of the Korean peninsula by the phytosociological methods. As a result of the investigation of 26 quadrats, the pine forest was classified two communities and six subcommunities : Ardisia japonica - Pinus densiflora community and lsodon japonicus - Pinus densiflora community, and Farfugium japonicum subcommunity, Quercus serrata subcommunity, Typical subcommunity of Ardisia japonica - P. densiflora community, Juniperus rigida subcommunity, Pourthiaea villosa subcommunity and Typical subcommunity of lsodon japonicus - P. densiflora community. The subsidiary knowledges from this study provide practical information to clarify the syntaxonomy of natural pine forest of the Korean peninsula by the phytosociological methods.

• The Korean Journal of Food And Nutrition
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• v.28 no.6
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• pp.1098-1106
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• 2015

This study aimed to investigate antioxidant activities from 11 forest plants, and determine their total phenolics, flavonoids and proantocyanidins contents. In addition, the antioxidant activities were correlated with antioxidant compounds. Among the samples, Cornus officinalis, Castanea crenata, Lindera erythrocarpa, Carpinus laxiflora and Pourthiaea villosa showed significantly higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) ($IC_{50}=21.12{\sim}28.93{\mu}g/mL$) and 2,2'-azino-bis(3-ethylbenzothia zoline-6-sulfonic acid) (ABTS) ($IC_{50}=28.18{\sim}52.55{\mu}g/mL$) radical scavenging ability with reducing power ($IC_{50}=59.91{\sim}93.64{\mu}g/mL$) than other plants; and C. crenata, L. erythrocarpa and Rubus coreanus showed strong nitric oxide (NO) inhibition activity (${\geq}60%$). In addition, L. erythrocarpa, C. laxiflora and P. villosa showed higher oxygen radical absorbance capacity (ORAC) values (${\geq}1,100{\mu}M$ TE/g sample) than other samples. High total phenolic contents were observed in C. crenata (429.11 mg GAE/g), L. erythrocarpa (437.11 mg GAE/g), C. laxiflora (408.67 mg GAE/g) and P. villosa (404.11 mg GAE/g). The DPPH and ABTS radical scavenging activity with reducing power were significantly correlated with total phenolic contents ($R^2=0.71{\sim}0.79$), but total phenolic contents were not correlated with NO inhibition and ORAC ($R^2=0.35{\sim}0.43$). Therefore, these results suggested that C. officinalis, C. crenata, L. erythrocarpa, C. laxiflora and P. villosa are potential natural antioxidative candidate ingredients.

• Korean Journal of Pharmacognosy
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• v.34 no.1 s.132
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• pp.100-108
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• 2003

The regulation of plasma lipid level, particularly LDL cholesterol, represents the focus of current therapy for atherosclerosis. And $Lp-PLA_2$ is able to hydrolyse oxidized phosphatidylcholine within LDL into lyso-PC and oxidized fatty acids. $Lp-PLA_2$ is a potential biomarker of coronary heart disease and plays an important proinflammatory role in the progression of atherosclerosis. We investigated the inhibitory effects of methanol extracts of 224 natural plants on $Lp-PLA_2$ activity. Seven kinds of methanol extracts of tested plants showed above 50% inhibitory effect with the concentration of $100\;{\mu}g/ml$. The concentrated aqueous suspensions of each methanol extract were partitioned with n-hexane, $CHCl_3$, and EtOAc. Among them, EtOAc extracts of Astilbe chinensis var. davidii (root) and Pourthiaea villosa var. brunnea (leaf) significantly inhibited $Lp-PLA_2$ activity at the same concentration.

• Journal of Environmental Science International
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• v.18 no.12
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• pp.1427-1436
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• 2009

The purpose of this study was to explain community structure for actual vegetation and their environment variables in Anmyeon Island. Samples were collected for 106 plots using ZM phytosociology method and coincidence method. Actual vegetation were classified into three vegetations types(forest vegetation type, maritime vegetation type, lake vegetation type) and eleven community units. Pourthiaea villosa community in forest vegetation type was divided into 5 groups such as Carpinus coreana group, Pinus rigida group, Chamaecyparis obtusa group, Castanea crenata group and Typical group. Maritime vegetation type was divided into 3 communities, such as Vitex rotundifolia community, Koelreuteria paniculata community and Suaeda japonica community. V. rotundifolia community was subdivided into 2 groups, Rosa rugosa group and Diodia teres group. K. paniculata community was subdivided into 2 groups, Grewia biloba var. parviflora group and Typical group. Lake vegetation type was divided into 1 community, Nelumbo nucifera community. And it was entirely classified into 11 community units.

• The Korean Journal of Ecology
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• v.25 no.3 s.107
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• pp.197-204
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• 2002

This study was carried out to analyze Pinus densiflora forest in islets of southwestern coast of the Korean peninsula by the phytosociological methods. As a result of the investigation of 26 quadrats, the pine forest was classified two communities and six subcommunities : Ardisia japonica-Pinus densiflora community and Isodon japonicus-Pinus densiflora community, and Farfugium japonicum subcommunity, Quercus serrata subcommunity, Typical subcommunity of Ardisia japonica-P. densiflora community, Juniperus rigida subcommunity, Pourthiaea villosa subcommunity and Typical subcommunity of Isodon japonicus-P. densiflora community. The subsidiary knowledges from this study provide practical information to clarify the syntaxonomy of natural pine forest of the Korean peninsula by the phytosociological methods.