• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.199-202
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• 2000

Optimization of submerged culture conditions for the production of exo-biopolymer from Paecilomyces japonica was studied. Maltose, yeast extract and potassium phosphate were the most suitable sources of carbon, nitrogen, and inorganic salt, respectively, for both production of the exo-biopolymer and mycelial growth. The optimal culture conditions in flask culture were pH 5.0, $25^{\circ}C$ and 150 rpm in a meidum containing of 30 g maltose, 6 g yeast extract, 2 g polypeptone, 0.5 g $K_2HPO_4$, 0.2 g $KH_2PO_4$, 0.2 g $MnSo_4\;{\cdot}\;5H_2O$, 0.2 g $MgSO_4\;{\cdot}\;7H_2O$ in 1-L distilled water. Exo-biopolymer production and mycelial growth in the suggested medium were significantly increased in a 2.5-L jar fermentor, where the maximum biopolymer concentration was 8 g/1.

• Journal of Veterinary Clinics
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• v.18 no.1
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• pp.74-77
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• 2001

A ten-year-old, male cat presented with recent loss of body weight, depression, vomiting, anorexia, polydipsia, and polyuria. General physical findings included depression, weakness, severe dehydration and a strong acetone odor on the breath. A complete blood count and serum biochemical profiles were leukocytosis, hyperglycemia (286 mg/dl), hypokalemia (2.6 mEq/L), hyponatremia, and high serum fructosamine (600 $\mu$mol/L). In blood gas analysis the cat had acidosis (pH 7.127, p$CO_2$26.7 mmHg). In urinalysis glycosuria and ketouria were appeared. On the basis of clinical signs, serum chemistry, blood gas analysis and urinalysis, diabetic ketoacidosis was diagnosed. Treatment included subcutaneous administration of protamine zinc insulin (0.75 U/head) and intravenous administration of 0.9% saline. Potassium phosphate and sodium bicarbonate was added to the fluids. Serum fructosamine for assessment of glycemic control was measured on occasion calls. On day 296, the patient improved clinically and did not experience any problems resulting from diabetic ketoacidosis.

• Microbiology and Biotechnology Letters
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• v.26 no.4
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• pp.365-368
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• 1998

The aklavinone 11-hydroxylase which was overexpressed using T7 promoter in E. coli could be detected in SDS-PAGE only in insoluble precipitate without any detectable enzyme activity. The insoluble enzyme was solubilized in 6M guanidine$.$HCl solution and their refolding ability was tested under various conditions. When the enzymatic activity was checked by the bioconversion experiment, stepwise dialysis against 6M, 3M, 1M guanidine$.$HCl and finally 100 mM potassium phosphate buffer of the solubilized protein gave the best bioconversion efficiency. The aklavinone 11-hydroxylase showed its enzymatic activity in the reaction buffer containing NADPH with vigorous shaking. The enzymatic activity was lost during partial purification and regained by the addition of crude extract of S. lividans in the reaction mixture. This effect was confirmed to due to some low-molecular weight component(s) in the crude extract, because the addition of dialyzed crude extract could not recover the enzymatic activity.

• Korean Journal of Soil Science and Fertilizer
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• v.8 no.3
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• pp.113-119
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• 1975

Volcanic Ash Soils are widely distributed in Jeju island, and constitute the important upland soils which are either presently being cultivated or are suitable for reclaiming. The characteristics of Volcanic Ash Soils according to data made available by previous studies in Jeju and the outside of the country are as following: The most conspicuous mineralogical property is the presence of amorphous mineral colloids. The colloids have large and highly reactive surface to which the common physical and chemical properties are related. Soils are low in bulk density and higher both in porosity and permeability. Accumulation of humus in the upper part of soil is found in great quantity. Cation exchange capacity is high mainly due to high humus content, but the absorbing intensity of ammonium and potassium is weaker than that of crystalline clays. The phosphate absorption coefficient is extremely high and deficiency of minor element may occur both crops and animals. Soils are densely populated with actinomycetes and anaerobic bacteria. Nitrification and activity of urease are distinctly stronger than that of non-Volcanic Ash Soils.

• Microbiology and Biotechnology Letters
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• v.9 no.2
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• pp.77-82
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• 1981

Candide tropicalis was selected for its ability to utilize spent waste generated by the alcohol distillery using tapioca starch as a raw material. Optimum pH and temperature on batch culture of the organism were 4.0 and 3$0^{\circ}C$. The growth of the organism was markedly increased when 0.2% of ammonium sulfate, 0.002% of potassium phosphate dibasic, add 0.04% of magnesium sulfate were supplemented to the filtrate. At these conditions, maximum specific growth rate and saturation constant were 1.0 hr$^{-1}$ and 4.4 g.1$^{-1}$ , respectively. At a dilution rate of 0.5hr$^{-1}$ , a productivity of 1.84 g.1$^{-1}$ . hr$^{-1}$ was obtained and about 70% of carbohydrate was assimilated. Protein content of dried cell was about 60%.

• The Korean Journal of Ecology
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• v.15 no.4
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• pp.387-394
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• 1992

Decomposition in the litteras of platanus orientalis was studied by month in the dam of seoul national university. total amounts of the litter had the tendency to decrease with the laps of the time. Those of crude protein and phosphate contents, however, increased much more than initial amounts. It was throught that the growth of aquatic microbes gave rise to increase them. Dry weight, organic matter and holocellulose tended to decreased gradually. But, the amount of calcium loss increased rapidly since march and then the amoung of potassium and sodium decreased slowly. It was thought that this changing pattern was ascribed to water temperature, the growth of aquaqtic microbes and the leaching of soluble parts by stream current.

• Journal of Chest Surgery
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• v.44 no.4
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• pp.311-313
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• 2011

Hemangiomas that arise in cervical esophagus are extremely rare, representing 3.3% of all benign esophageal tumors. Although endoscopic mucosal resection (EMR) and potassium titanyl phosphate/yttrium aluminum garnet (KTP/YAG) laser therapy have been used with success for small tumors, the safety and efficacy in the case of large tumors remains uncertain. We report the successful resection of cervical esophageal hemangioma through a cervical esophagotomy in a patient with thyroid cancer who needed a cervical collar incision.

• Microbiology and Biotechnology Letters
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• v.25 no.5
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• pp.506-511
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• 1997

For the production of benzoylformic acid from DL-phenylglycine, 8 strains of bacteria capable of producing benzoylformic acid were isolated from soil. Among them, the strain YH-001 showed the highest activity for production of benzoylformic acid, and was partially identified as a Bacillus sp. For the production of benzoylformic acid, a reaction mixture and reaction conditions were optimized as follows: the reaction mixture contained 2 g of DL-phenylglycine and 50 g of wet cells in 1 l of 50 mM potassium phosphate buffer (pH 8.0) and the reaction was carried out at 40$circ$C with shaking. After 6 hr incubation, 1.01 g/l of benzoylformic acid was produced which coffesponded to a conversion yield of 50.5%.

• Archives of Pharmacal Research
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• v.14 no.2
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• pp.99-102
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• 1991

A hight performance liquid chromatographic methods was developed for the seperation and determination of seven alkaloids in "sanjoin" (the seeds of Zizyphus jujuba Rhamnaceae), a plant with potent sedative activity. A reverse phase system of Lichrosorb RP-Select B column and 0.05 M potassium phosphate buffer (pH = 3.5)-acetonitrile with gradient elution was employed. Two known alkaloids, juzirine and lysicamine, were newly isolated fom "sanjoin"."sanjoin".oin&".ot;.

• The Korean Journal of Ecology
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• v.17 no.4
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• pp.425-434
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• 1994

After two highly weathered soils were treated with glucose, ammonium nitrate, monobasic potassium phosphate and biocides, and incubated for 4 or 6 weeks, adsorption tests were carried out to determine their effect on P adsorption. Glucose addition generally decreased P adsorption. The addition stimulated microbial activity, which might contribure to the reduced adsorption, probably through chelation and anion competition. Consistent endency was not observed with N treatment. Addition of P initially decreased P adsorption, probably through blockage of adsorption sites. Biocides generally decreased adsorption, probably because the microbes that 몬 been killed. Soil 1 with naturally lower levels of C and higher levels of aluminium adsorbed more P than soil 2. These results suggest that in highly weathered soils, which are low in available P and high in exchangeable Al, cultivation techniques which increase soil organic matter will also result in higher levels of plant-available P.