Bacillus sp. YH-001에 의한 DL-Phenylglycine으로부터 Benzoylformic acid 의 생산

  • 박윤희 (고려대학교 자연자원대학 농화학과) ;
  • 이일석 (고려대학교 자연자원대학 농화학과) ;
  • 방원기 (고려대학교 자연자원대학 농화학과)
  • Published : 1997.10.01

Abstract

For the production of benzoylformic acid from DL-phenylglycine, 8 strains of bacteria capable of producing benzoylformic acid were isolated from soil. Among them, the strain YH-001 showed the highest activity for production of benzoylformic acid, and was partially identified as a Bacillus sp. For the production of benzoylformic acid, a reaction mixture and reaction conditions were optimized as follows: the reaction mixture contained 2 g of DL-phenylglycine and 50 g of wet cells in 1 l of 50 mM potassium phosphate buffer (pH 8.0) and the reaction was carried out at 40$circ$C with shaking. After 6 hr incubation, 1.01 g/l of benzoylformic acid was produced which coffesponded to a conversion yield of 50.5%.

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