• Title/Summary/Keyword: Pot life

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Production of Antifungal Compost by Using Bacillus licheniformis KJ-9 (Bacillus licheniformis KJ-9를 이용한 항균발효퇴비의 생산)

  • Seo, Dong-Cheol;Ko, Jeong-Ae;Lee, Sang-Won
    • Journal of Life Science
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    • v.20 no.9
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    • pp.1339-1344
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    • 2010
  • In order to produce environmental-friendly fermented compost, a cattle manure-sawdust compost (antifungal compost) was developed by inoculation of B. licheniformis KJ-9 to cattle manure-sawdust. The thermal stability of the antifungal substance produced by B. licheniformis KJ-9 maintained more than 60% antifungal activity with heat treatment at $100^{\circ}C$ for 10 min, and the optimum pH of antifungal activity of the substance was 7.0. In a pot experiment with red pepper, the antifungal compost increased 1.5~2 times in leaf number and stem and root growth rate compared to those of commercial compost. Also, the diameter of stems increased 1.5-3 times in the antifungal compost treated group. The amount of microbes increased markedly in soil supplemented with antifungal compost compared to the control. In the field experiment for cultivation of garlic and Perilla japonica, the growth of both crops was significantly enhanced in the field treated with antifungal compost as compared to the commercial compost.

Effect of Sewage Sludge Application on Growth of Corn and Chinese Cabbage and Chemical Properties of Soil (하수오니 시용이 옥수수 및 배추 생육과 토양의 화학성 변화에 미치는 영향)

  • Lee, Seung-Heon;Park, Mi-Hyun;Yoo, Sun-Ho;Kim, Kye-Hoon
    • Korean Journal of Soil Science and Fertilizer
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    • v.33 no.6
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    • pp.463-471
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    • 2000
  • A pot experiment was carried out to find out the effects of the sludge application on corn and Chinese cabbage growth and changes in soil chemical properties with sludges collected from 4 plants at 0, 12.5, 25, 50, and $100Mg\;ha^{-1}$ levels and chemical fertilizer. With the corn experiment, the pot where sludge and chemical fertilizers were treated together, greater amount of sludge resulted in initial growth inhibition. In general, higher sludge treatment rates resulted in better growth in the end, whereas initial growth was inhibited due to high the electrical conductivity of saturated extracts(ECe) for the Chinese cabbage. However, the highest yield among sludge treatments was lower than the yield with chemical fertilizers. While the treatments resulted in chemical changes in soil showing differences of cation exchange capacity, organic matter contents, and nitrogen contents, hardly any changes were detected before and after crops were grown. Inorganic nutrients such as Na, K, Ca and Mg showed similar changes. The ECe in soil saturation extract decreased after crops were grown. The more sludge was treated, the greater was the decrease. The differences of ECe in the soil saturation extract with varying degrees of treatment were also reduced after crops were grown. Available phosphorus content increased during growth. Due to the low nitrogen content in sludge, when nitrogen becomes the determining factor for the amount of sludge treatment, phosphorus buildup resulted from continued application of sludge could be raised. Therefore, it is advisable to use phosphorus, not nitrogen content, in determining the amount of sludge treatment and chemical fertilizer as supplementary sources for nitrogen.

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Effect of Azospirillum brasilense and Methylobacterium oryzae Inoculation on Growth of Red Pepper (Capsicum annuum L.)

  • Chung, Jong-Bae;Sa, Tong-Min
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.1
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    • pp.59-65
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    • 2012
  • Plant growth-promoting effects of rhizobacterial inoculation obtained in pot experiments cannot always be dependably reproduced in fields. In this study, we investigated the effect of inoculation with Azospirillum brasilense and Methylobacterium oryzae, which have displayed growth promoting effects in several pot experiments, on growth and fruit yield of red pepper under field condition in a plastic-film house. Four rows spaced 90 cm apart were prepared after application of compost ($10Mg\;ha^{-1}$), and red pepper seedlings (Capsicum annum L., Nocgwang) were transplanted in each row with 40-cm space. Experimental treatments were consisted of A. brasilense CW903 inoculation, M. oryzae CBMB20 inoculation, and uninoculated control. Twelve plots, 10 plants per plot, were allotted to the three treatments with four replicates in a completely randomized design. At the time of transplanting, 50 mL of each inoculum ($1{\times}10^8cells\;mL^{-1}$) was introduced into root zone soil of each plant, and re-inoculated at 7 and 14 days after transplant. Plant growth and fruit yield were measured during the experiment. Both A. brasilense CW903 and M. oryzae CBMB20 could not promote growth of red pepper plants. All growth parameters measured were not significantly different among treatments. There were large variations in fruit yield recorded on plot basis, and no statistically significant differences were found among treatments. The failure to demonstrate the expected plant growth promoting effect of the inoculants is possibly due to various environmental factors, including weather and soil characteristics, reducing the possibility to express the potential of the inoculated bacterial strains.

Molecular breeding of herbicide resistant transgenic plants with bromoxynil specific nitrilase gene (Bromoxynil 특이성 nitrilase 유전자를 이용한 제초제 저항성 형질 전환 식물의 분자육종)

  • Min, Bok-Kee;Park, Eun-Sung;Park, Yearn-Hung;Song, Jae-Young;Lee, Se-Yong
    • Applied Biological Chemistry
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    • v.37 no.4
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    • pp.248-254
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    • 1994
  • Bromoxynil is an antidicot herbicide widely used on cereal crops and has a short half life in the soil. A bxn gene, encoding a specific nitrilase that converts bromoxynil to its primary metabolite 3,5-dibromo-4-hydroxybenzoic acid, was inserted in plant binary vector pGA482, and then introduced into tobacco and lettuce plants via Agrobacterium mediated leaf-disc transformation method. Transgenic plants with the bxn gene were selected by kanamycin and regenerated to whole plants. The regenerated transgenic plants were determined level of expression of bxn gene by Northern blot analysis. Leaf-disc analysis and pot-assay confirmed that the transgenic tobacco and lettuce plants were resistant to high doses of bromoxynil.

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Screening of Antifungal Bacillus spp. against Alternaria Blight Pathogen (Alternaria panax) and Anthracnose Pathogen (Colletotrichum gloeosporioides) of Ginseng (인삼 점무늬병균(Alternaria panax)과 탄저병균(Colletotrichum gloeosporioides)에 대한 길항미생물 Bacillus spp. 선발)

  • Lee, Hye-Jin;Park, Kee-Choon;Lee, Seung-Ho;Bang, Kyong-Hwan;Park, Hong-Woo;Hyun, Dong-Yun;Kang, Seung-Weon;Cha, Seon-Woo;Chung, Ill-Min
    • Korean Journal of Medicinal Crop Science
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    • v.20 no.5
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    • pp.339-344
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    • 2012
  • This study was carried out to research microorganisms having the antifungal activity against ginseng Alternaria blight pathogen Alternaria panax and ginseng anthracnose pathogen Colletotrichum gloeosporioides. Eleven Bacillus strains. were isolated from Korean traditional soybean paste and Kimchi. Among the 11 isolates, DJ5, KC1, KC2 and KC4 showing antagonistic activity on the mycelial growth of A. panax and C. gloeosporioides in pairing culture were finally selected as the antagonistic microorganisms. Based on 16s rRNA sequence and phylogenetic tree analysis, they were identified as Bacillus spp.. The selected microorganisms were investigated antagonistic activity by measured leaf-segment colonization in pot test. When Bacillus sp. were injected after A. panax treatment, KC1, KC2 and KC4 showed similar effect to chemical pesticides treated control. To measure preventive effect of Bacillus sp, antagonistic microorganisms were injected and C. gloeosporioides was treated in pot. When measuring the effectiveness for the prevention of Anthracnose, All Bacillus spp. showed approximately 83~90 % degree of superior preventive effect. In general, The four Bacillus spp. isolated from Korean traditional fermented foods showed therapeutic effect of Alternaria blight and preventive effect of Anthracnose.

Studies on ecolgy and control for the purplish stem borer (Sesamia inferens Walker) in Korea (한국에서의 벼밤나방(Sesamia inferens Walker)의 생태와 약제방제에 관한 연구)

  • Bae S. H.;Lee J. O.;Lee B. H.
    • Korean journal of applied entomology
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    • v.7
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    • pp.27-32
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    • 1969
  • The purplish stem borer (Sesamia inferens Walker). which was infested in southern part of Korea. attack rice plant. and its damage appear quite serious in some districts. This study was conducted to investigate the life history. seasonal abundance and control of the insect pest. The pest overwinter as larvae and it seems to have 2-3 generations in a year. It takes 56.6 days in the shortest one while the longest one takes 74.6 days. Three peaks of moth appearance are May-June. end of July and end of August, respectively. The effectivencess of insecticides was compared with EPNec $45\%$ and the different granular insecticides in pot experiment. In the result. M. Parathion G. EPNec, Diazinon G and Lebaycid G showed good control of the pest. with the infestation of $12.0,\; 22.9\%,\; 24.7\%,\; and\; 27.3\%$ respectively, while the untreated has $62.6\%$

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Antagonistic Effect of Lactobacillus sp. Strain KLF01 Against Plant Pathogenic Bacteria Ralstonia solanacearum (세균성 시들음병에 대한 식물성 유산균(Lactobacillus sp.)의 저해효과)

  • Shrestha, Anupama;Choi, Kyu-Up;Lim, Chun-Keun;Hur, Jang-Hyun;Cho, Sae-Youll
    • The Korean Journal of Pesticide Science
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    • v.13 no.1
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    • pp.45-53
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    • 2009
  • An antagonistic bacterial strain KLF01 was isolated from rhizosphere of tomato and identified to be Lactobacillus sp. by biochemical and genetic analysis. This strain showed antagonism against the used plant pathogenic bacteria like Ralstonia solanacearum, (bacterial wilt), Xanthomonas axonopodis pv. citri, (Citrus canker), Xanthomonas campestris pv. vesicatoria (Bacterial spot), Eriwinia pyrifoliae (Shoot-blight) and Eriwinia carotovora subsp. carotovora group (Potato scab) through agar well diffusion method. In planta test done by drench application of strain KLF01 $(4{\times}10^8 cfu/ml)$ into the experimental plot containing tomato (Solanum lycopersicum L.) cultivar 'Lokkusanmaru' and red pepper (Capsicum annuum L.) cultivar 'Buja' plants, in pot test post-inoculated with the plant pathogenic bacteria, R. solanacearum significantly reduced the disease severity, compared to the non-treated plants.

Propagation by In Vitro Zygotic Embryos Cultures of the Quercus myrsinifolia

  • Choi, Eun ji;Yong, Seong Hyeon;Seol, Yu Won;Park, Dong Jin;Park, Kwan Been;Kim, Do Hyun;Jin, Eon Ju;Choi, Myung Suk
    • Journal of Forest and Environmental Science
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    • v.37 no.4
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    • pp.323-330
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    • 2021
  • Zygotic embryo culture was performed to propagate evergreen oak, Quercus myrsinifolia, which has recalcitrant seeds and is difficult to propagate by cuttings. Zygotic embryos appeared in WPM medium after 14 days, and after 56 days, they developed into complete plants with cotyledons and roots. The medium suitable for zygotic embryo culture was 1/4 WPM medium, showing a shoot growth of 2.43 cm and root growth of 8.7 cm after 8 weeks of culture. As a result of investigating the effect of GA3 on the growth of plants germinated from zygotic embryos through GA3 treatment, the best growth was shown in 0.5 mg/l GA3 treatment. The in vitro rooting and growth of IBA-treated zygotic embryo-derived plants were good in the 0.5 mg/l IBA treatment and rooting and shoot growth were not observed at higher concentrations. And the callus induction rate also increased as the concentration of IBA increased. Plants grown in vitro were transferred to a plastic pot containing artificial soil and acclimatized in a greenhouse for about 4 weeks, resulting in more than 90% survival. As a result of this study, the zygotic embryo culture method was confirmed to be effective for mass propagation of Q. myrsinifolia. The results of this study are expected to contribute significantly to the mass propagation of elite Q. myrsinifolia.

A Study on the Cooking in 'The Kosa-sibi Jip' (2) ("고사십이집(攷事十二集)"의 조리가공에 관한 분석적 연구(2))

  • 김성미
    • Journal of the East Asian Society of Dietary Life
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    • v.4 no.3
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    • pp.1-19
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    • 1994
  • In this paper, twenty-five kinds of food presented in Sooljip(戌集) 5 and 6 of Food collections of 'Kosa-sibi Jip(攷事十二集)' have been classified into four : Staple food, subsidiary food, Tuck(rice cake) and Han-gwa(Korean confectionery), and Tang-jng and tea. Cooking processes have been examined and scientifically analyzed in terms of cooking, Fourteen kinds of Jook (thick gruel with cereal) as well as Urak-Jook were presented among the methods of making Jook, one of staple foods. Milk and ground rice were boiled together into Urak-Jook, which was nutritious because of carbohydrate, added to milk. Hong-sa Myun was mode of ground shrimps, ground bean, ground rice and flour which were kneaded together. It was a nutritiously balanced food. Nineteen kinds of Kimchi presented in this book were classified by the recipes. The five of Jook-soon Ja, U-so Ja, Tam-bok Ja and Jo-gang were made by adding red malt and cereals(boiled rice or candies). Jo-gang, Jo-ga and Jo-gwa-chae were made by adding salt and rice wine. With salt and fermenters added, eight were made. Chim-jup-jeo-ga was made by adding Jang(soy-bean sauce) and the inner chaff of wheat instead of salt. The four of Ka-za-san, Hwang-gwa-san, Tong-gwa-san and Jo-gang were made by adding salt and vinegar. Jo-gang was made by adding salt, rice wine, residue of rice wine and candies. The four of Kae-mal-ga, Ku-cho-chim-chae, Un-gu-hwa and Suk-hwa-chim-chim-chae were made by adding salt and spices. San-got-Kimchi was made without salt. San-got-Kimchi and Suk-hwa-chim-chae were made originally in Korea. Suk-hwa-chim-chae, in particular, was first classified as a kind of Kimchi in this book and oysters were added, which is notable. Pork could be preserved longer when smoked oven the weak fire of thatch ten days and nights. Dog meat was sauced and placed on the bones in a pot. A porcelain was put on the top of the pot. Flour paste sealed the gap between the porcelain and the pot. Some water was poured into the porcelain, and the meat was steamed, with two or three thatched sacks burned, which was a distilled dry steaming. This process has been in use up to now. Various cooking methods of chicken were presented from in Umsik-dimi-bang to in Chosun Musang Sinsik Yori Jebup. These methods were ever present regardless of ages. Such measuring units as Guin(斤) and Nyang(兩) were most frequently used in cooking processes of this book, except in case of Jang(soy bean sauce), vinegar and liquor. Twenty eight kinds of kitchenware and cookers were used, of which porcelains wee most used and pans and sieves followed. The scientific eight cooking methods were as follows. First, salt was refined through saturated solution. Next, it was recommended Hong-sa Myun containing shrimps should not be taken along with pork, which is thought to be a proper diet in terms of cholesterol contained by shrimps and pork. Third, meat was coated with thin gruel and quickly roasted and cleared of the dried gruel membrane, which prevented nutrients from exuding and helped to make the meat well-done. Fourth, The fruit of paper mulberry trees has the protease which can soften meat. Therefore when meat was boiled with th fruit of paper mulberry trees, it can be softened easily. Fifth, pork was smoked over the weak fire of thatch. Sixth, in cooking dog meat, distilled dry steaming raised the boiling point and made it possible to preserve meat longer. Seventh, in boiling the sole of a bear, lime was added, which made meat tender by making the pH lower or higher than that of raw meat. Finally, in boiling down rice gluten, a porcelain in the pot prevented boiling over the brim, which is applied to pots in which to boil medical herbs.

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Selection Indices to Identify Drought-tolerance and Growth Characteristics of the Selected Korean Native Plants (자생식물로부터 내건성 식물의 최적인자 선발과 생육특성)

  • Im, Hyeon Jeong;Song, Hyeon Jin;Jeong, Mi Jin;Seo, Yeong Rong;Kim, Hak Gon;Park, Dong Jin;Yang, Woo Hyung;Kim, Yong Duck;Choi, Myung Suk
    • Journal of agriculture & life science
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    • v.50 no.2
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    • pp.73-82
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    • 2016
  • Best drought tolerance index was determined through statistics analysis and growth appearance of drought tolerant plants was determined by cultivation in pot and sloping land. For determination of best drought tolerant indicators, RD(Resistant dry days), LD(Leaf area), UTR(Unit transpiration), RWC(Relative water content), RWL(Relative water loss), LA(Leaf area), SN(Stoma unmber) and SA(Stoma area) were carried out by correlation and PCA analysis. RWL and UTR were affected on plant drought tolerance according to comparison among six indices for resistant dry days. The PCs axes separated SA, LA, RD and RWC and SN. UTR was negatively correlated with SA, RWL were also negatively correlated with RWC and SN. RWL and UTR were proved best selection indicator for the selection of drought tolerant species. Ulmus parvifolia, Bidens bipinnata, Patrinia villosa, Kummerowia striata, Arundinella hirta, Artemisia gmelini etc. were selected drought tolerant plants. Shoot growth appearance of drought resistant plants was differed pot and sloping land. Shoot growth and leaf number was no significant differences between the pot and sloping land. However, root growth of drought tolerant plants was all the difference between two cultivation. T/R ratio of drought tolerant plants was also found a big difference. T/R ratio of drought tolerant plants in sloping land was lower than that of pot. These results will be served efficiently plant breeding.