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http://dx.doi.org/10.7783/KJMCS.2012.20.5.339

Screening of Antifungal Bacillus spp. against Alternaria Blight Pathogen (Alternaria panax) and Anthracnose Pathogen (Colletotrichum gloeosporioides) of Ginseng  

Lee, Hye-Jin (Department of Herbal Crop Research, NIHHS, RDA)
Park, Kee-Choon (Department of Herbal Crop Research, NIHHS, RDA)
Lee, Seung-Ho (Department of Herbal Crop Research, NIHHS, RDA)
Bang, Kyong-Hwan (Department of Herbal Crop Research, NIHHS, RDA)
Park, Hong-Woo (Department of Herbal Crop Research, NIHHS, RDA)
Hyun, Dong-Yun (Department of Herbal Crop Research, NIHHS, RDA)
Kang, Seung-Weon (Department of Herbal Crop Research, NIHHS, RDA)
Cha, Seon-Woo (Department of Herbal Crop Research, NIHHS, RDA)
Chung, Ill-Min (Department of Applied Bioscience, College of Life and Environmental Science, Konkuk University)
Publication Information
Korean Journal of Medicinal Crop Science / v.20, no.5, 2012 , pp. 339-344 More about this Journal
Abstract
This study was carried out to research microorganisms having the antifungal activity against ginseng Alternaria blight pathogen Alternaria panax and ginseng anthracnose pathogen Colletotrichum gloeosporioides. Eleven Bacillus strains. were isolated from Korean traditional soybean paste and Kimchi. Among the 11 isolates, DJ5, KC1, KC2 and KC4 showing antagonistic activity on the mycelial growth of A. panax and C. gloeosporioides in pairing culture were finally selected as the antagonistic microorganisms. Based on 16s rRNA sequence and phylogenetic tree analysis, they were identified as Bacillus spp.. The selected microorganisms were investigated antagonistic activity by measured leaf-segment colonization in pot test. When Bacillus sp. were injected after A. panax treatment, KC1, KC2 and KC4 showed similar effect to chemical pesticides treated control. To measure preventive effect of Bacillus sp, antagonistic microorganisms were injected and C. gloeosporioides was treated in pot. When measuring the effectiveness for the prevention of Anthracnose, All Bacillus spp. showed approximately 83~90 % degree of superior preventive effect. In general, The four Bacillus spp. isolated from Korean traditional fermented foods showed therapeutic effect of Alternaria blight and preventive effect of Anthracnose.
Keywords
Ginseng; Bacillus; Antagonistic Microorganism; Alternaria panax; Colletotrichum gloeosporioides; Biological Control;
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Times Cited By KSCI : 7  (Citation Analysis)
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1 Bae YS, Park BY, Kang SW, Cha SW, Hyun KS, Yeon BR, An TJ, Lee SW, Hyun DY, Kim YC, Chung KC, Kim SK and Han MJ. (2005). Handbook of ginseng diseases and pests. National Institute of Crop Science Press. Suwon, Korea. p.1-79.
2 Baker CJ, Stavely JR, Tomas CA, Myron S, Janet S and Macfall S. (1983). Inhibitory effect of Bacillus subtilis on Uromyces phaseoli and on leaves development of rust pustules on bean leaves. Journal of Phytopathology. 73:1148-1152.   DOI
3 Cho JI, Cho JY, Yang SY, Park YS and Heo BG. (2007). Screening and identification of antifungal bacillus sp. #72 against the pathogenic stem-end rot of kiwi fruit. The Korean Journal of Community Living Science. 18:241-246.
4 Chung KC, Kim CB, Kim DK and Kim BJ. (2006). Isolation of antagonistic bacteria against major diseases in Panax ginseng C. A. Meyer. Korean Journal of Medicinal Crop Science. 14:202-205.
5 Gillis CN. (1997). Panax ginseng pharmacology: A nitiric oxide link. Biochemical Pharmacology. 54:1-8.   DOI   ScienceOn
6 Glick BR. Chen Z, Czarny J and Duan J. (2007). Promotion of plant growth by ACC deaminase-producing soil bacteria. European Journal of Plant Pathology. 119:329-339.   DOI
7 Han OK, Lee ET and Kim SD. (2001). Chitinase of multifunctional antagonistic bacterium Bacillus amyloliquefaciens 7079 against phytopathogenic fungi. Korean Journal of Microbiology and Biotechnology. 29:142-148.
8 Hu SY. (1976). The genus panax(Ginseng) in chinese medicine. Economic Botany. 30:11-28.   DOI
9 Jung WC, Shin TS, Kim BS, Im JS, Lee JH and Kim JW. (2008). Efficacy of antagonistic bacteria for biological control of Rhizoctonia blight (Large patch) on zoysiagrass. Research in Plant Disease. 14:43-50.   DOI
10 Kim YH, Yu YH and Lee JH. (1990). Effect of shading on the quality of raw, red and white ginseng and the contents of some minerals in ginseng roots. Journal of Ginseng Research. 14:36-43.
11 Kim YS, Lee MS, Yeom JH, Song JG, Lee IK, Yeo WH and Yun BS. (2012). Screening of antagonistic bacteria for biological control of ginseng root rot. The Korean Journal of Mycology. 40:44-48.   DOI
12 Lee BD and Park H. (2004). Control effect of Bacillus subtilis B-4228 on root rot of Panax ginseng. Journal of Ginseng Research. 28:67-70.   DOI
13 Lee GW, Kim MJ, Park JS, Ju JE, Lee KJ, Chae JC and Soh BY. (2011). Biological control of phytophthora blight and anthracnose disease in red-pepper using Bacillus subtilis S54. Research in Plant Disease. 17:86-89.   DOI
14 Lee JH, Yu YH, Kim YH, Ohh SH and Park WM. (1990). Morphological characteristics and pathogenicity of Alternaria isolates causing leaf and stem blight and black root rot of Korea ginseng. Plant Pathology Journal. 6:13-20.
15 Lee SW, Kim, GS, Hyun DY, Kim YB, Kang SW and Cha SW. (2010). Effects of spraying lime-bordeaux mixture on yield, ginsenoside, and 70% ethanol extract contents of 3-yearold ginseng in Panax ginseng C. A. Meyer. Korean Journal of Medicinal Crop Science. 18:244-247.
16 Lee WH, Kim JH and Choi IY. (2008). Advantages and disadvantage in using biological control of plant diseases and integrated control. Journal of Agriculture & Life Science. 39:66-76.
17 Lim SY, Kim JD, Kim BS and Hwang BK. (2000). Isolation and numerical identification of Streptomyces humidus strain S5-55 antagonistic to plant pathogenic fungi. Plant Pathology Journal. 16:189-199.
18 Nam KY, Park JD and Choi JE. (2011). Radioprotective potential of Panax ginseng: current status and future prospectives. Korean Journal of Medicinal Crop Science. 19:287-299   DOI
19 Mehnaz S, Mirza MS, Hanrat J, Bally R, Normand P, Bano A and Malik KA. (2001). Isolation and 16S rRNA sequence analysis of the beneficial bacteria from the rhizosphere of rice. Canadian Journal of Microbiology. 47:110-117.   DOI
20 Nei M and Kumar S. (2000). Molecular evolution and phylogenetics. Oxford University Press. New York City. New York, USA. p.1-333.
21 Msadek T, Dartois V, Kunst F, Herbaud ML, Denizot F and Rapoport G. (1998). ClpP of Bacillus subtilis is required for competence development, motility, degradative enzyme synthesis, growth at high temperature and sporulation. Molecular Microbiology. 27:899-914.   DOI   ScienceOn
22 Park EH, Kim DR, Park SK and Chang MS. (2012). Effects of ginseng radix on sperm count and CatSper3, 4 proteins expression in male mice. Korean Journal of Herbology. 27:47-51.   DOI
23 Reysenbach AL, Giver LJ, Wickham GS and Pace NR. (1992). Differential amplification of rDNA genes by polymerase chain reaction. Applied and Environmental Microbiology. 58:3417-3418.
24 Saitou N and Nei M. (1987). The neighbor-joining method: anew method for reconstructing phylogenetic trees. Journal of Molecular Biology. 4:406-425.
25 Tamura K, Dudley J, Nei M and Kumar S. (2007). MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version4.0. Molecular Biology and Evolution. 24:1596-1599.   DOI   ScienceOn
26 Woo SY, Lee DS and Kim PG. (2004). Growth and ecophysiological characteristics of identification of Panax ginseng grown under three different forest type. Journal of Plant Biology. 473:230-235.
27 Yong YL, Kim JH, Kim BS, Jeon JY and Yun CS. (2003). Effects of beneficial antagonists (Bacillus sp., Pseudomonas sp., and Trichoderma sp.) on control of clubroot of Chinese cabbage. Korean Journal of Horticultural Science & Technology. 21:194-198.