• 한국건설관리학회논문집
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• 제10권6호
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• pp.127-134
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• 2009

본 연구에서는 여러 대의 타워 크레인이 다양한 후보지점을 갖고, 자재 역시 다양한 야적 후좌지점을 가질 때 자재 운반 최적화를 지원하기 위한 유전자 알고리즘 기반의 모델을 제시하고자 한다. 대형 건축공사에서 타워 크레인의 위치와 자재 야적 위치의 변화는 자재 운반시간의 변화를 가져온다. 또한 여러 대의 타워 크레인을 사용하는 경우 각 자재의 운반에 어떠한 타워 크레인을 배정하느냐에 따라 작업의 효율성이 변화한다. 따라서 본 연구에서는 다중의 타워 크레인 설치 후보지, 여러 종류의 자재, 자재 야적 후보지점간의 다양하고 복잡한 상관관계를 다루기 위하여 유전자 알고리즘을 적용한 다중 양중장비 및 자재 야적 위치 최적화모델을 제시하였다. 또한, 제시된 모델을 사례에 적용하여 적용 과정을 예시하고 활용성을 검증하였다.

• 한국축산식품학회지
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• 제32권6호
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• pp.776-782
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• 2012

Marbling (intramuscular fat) is the most economically important meat quality trait in Hanwoo (Korean cattle). The endothelial differentiation G-protein coupled receptor 1 (EDG1) gene, involved in blood vessel formation, is located within the genomic region of a quantitative trait locus (QTL) for marbling on bovine chromosome 3. Thus, the EDG1 gene can be considered as a positional and functional candidate gene for meat quality in beef cattle. This study aimed to identify single nucleotide polymorphisms (SNPs) in the EDG1 gene and to evaluate their associations with carcass traits in Hanwoo population. We have sequenced a fragment of 5'-UTR of the EDG1 gene and identified one SNP. Genotyping of the g.166A>G SNP marker was carried out using PCR-RFLP analysis in 309 Hanwoo steers in order to evaluate their association with carcass traits. The g.166A>G SNP marker showed a significant effect on the marbling score. Animals with the GG genotype had higher marbling score compared with AA and AG genotypes (p<0.05). This SNP marker also showed a significant additive effects for the marbling score (p<0.05). These results suggest that the EDG1 gene can be used as a molecular marker for DNA marker-assisted selection in order to increase the levels of the marbling score in Hanwoo.

• Asian-Australasian Journal of Animal Sciences
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• 제20권9호
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• pp.1334-1341
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• 2007

This study identified hepatic differentially expressed genes (DEGs) affecting the marbling of muscle. Most dietary nutrients bypass the liver and produce plasma lipoproteins. These plasma lipoproteins transport free fatty acids to the target tissue, adipose tissue and muscle. We examined hepatic genes differentially expressed in a differential-display reverse transcription-polymerase chain reaction (ddRT-PCR) analysis comparing high- and low-marbled Hanwoo steers. Using 60 arbitrary primers, we found 13 candidate genes that were upregulated and five candidate genes that were downregulated in the livers of high-marbled Hanwoo steers compared to low-marbled individuals. A BLAST search for the 18 DEGs revealed that 14 were well characterized, while four were not annotated. We examined four DEGs: ATP synthase F0, complement component CD, insulin-like growth factor binding protein-3 (IGFBP3) and phosphatidylethanolamine binding protein (PEBP). Of these, only two genes (complement component CD and IGFBP3) were differentially expressed at p<0.05 between the livers of high- and low-marbled individuals. The mean mRNA levels of the PEBP and ATP synthase F0 genes did not differ significantly between the livers of high- and low-marbled individuals. Moreover, these DEGs showed very high inter-individual variation in expression. These informative DEGs were assigned to the bovine chromosome in a BLAST search of MS marker subsets and the bovine genome sequence. Genes related to energy metabolism (ATP synthase F0, ketohexokinase, electron-transfer flavoprotein-ubiquinone oxidoreductase and NADH hydrogenase) were assigned to BTA 1, 11, 17, and 22, respectively. Syntaxin, IGFBP3, decorin, the bax inhibitor gene and the PEBP gene were assigned to BTA 3, 4, 5, 5, and 17, respectively. In this study, the in silico physical maps provided information on the specific location of candidate genes associated with economic traits in cattle.

• Asian-Australasian Journal of Animal Sciences
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• 제25권12호
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• pp.1649-1659
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• 2012

Crosses between Korean and Landrace pigs have revealed a large quantitative trait loci (QTL) region for fat deposition in a region (89 cM) of porcine chromosome 4 (SSC4). To more finely map this QTL region and identify candidate genes for this trait, comparative mapping of pig and human chromosomes was performed in the present study. A region in the human genome that corresponds to the porcine QTL region was identified in HSA1q21. Furthermore, the LMNA gene, which is tightly associated with fat augmentation in humans, was localized to this region. Radiation hybrid (RH) mapping using a Sus scrofa RH panel localized LMNA to a region of 90.3 cM in the porcine genome, distinct from microsatellite marker S0214 (87.3 cM). Two-point analysis showed that LMNA was linked to S0214, SW1996, and S0073 on SSC4 with logarithm (base 10) of odds scores of 20.98, 17.78, and 16.73, respectively. To clone the porcine LMNA gene and to delineate the genomic structure and sequences, including the 3'untranslated region (UTR), rapid amplification of cDNA ends was performed. The coding sequence of porcine LMNA consisted of 1,719 bp, flanked by a 5'UTR and a 3'UTR. Two synonymous single nucleotide polymorphisms (SNPs) were identified in exons 3 and 7. Association tests showed that the SNP located in exon 3 (A193A) was significantly associated with weight at 30 wks (p<0.01) and crude fat content (p<0.05). This association suggests that SNPs located in LMNA could be used for marker-assisted selection in pigs.

• Asian-Australasian Journal of Animal Sciences
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• 제20권2호
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• pp.172-177
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• 2007

Thyroid hormones play an important role in regulating metabolism and can affect homeostasis of fat depots. The gene encoding thyroglobulin (TG), producing the precursor for thyroid hormones, has been proposed as a positional and functional candidate gene for a QTL with an effect on fat deposition. The SNP occurs in the 5' promoter region of the TG gene and is widely used in marker assisted selection (MAS) programs to improve the predictability of marbling level and eating quality in beef cattle. In this study, we identified three SNPs at the 5' promoter region of the TG gene in Korean cattle. Of the three SNPs identified in TG gene, the C257T and A335G were previously unreported new SNPs. The sequence data were submitted to GenBank (GenBank accession number: AY615525). The previously reported C422T SNP showed three genotypes, CC, CT and TT, by digestion with the restriction enzyme MflI using the PCR-RFLP method. A new allelic variant corresponding to the C${\rightarrow}$T and A${\rightarrow}$G mutations at positions 257 and 335, respectively, could be detected by the SSCP analysis. The gene-specific SNP marker association analysis indicated that the C422T SNP marker was significantly associated (p<0.05) with marbling score. Animals with the CC and CT genotypes had higher marbling score than those with the TT genotype. Results from this study suggest that TG gene-specific SNP may be a useful marker for meat quality traits in future MAS programs in Korean cattle.

• Asian-Australasian Journal of Animal Sciences
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• 제20권11호
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• pp.1662-1669
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• 2007

The high mobility group AT-hook1 (HMGA1) proteins are known to be related to the regulation of gene transcription, replication and promotion of metastatic progression in cancer cells. The loss of expression by disrupting the HMGA1 gene affects insulin signaling and causes diabetes in the mouse. Previously identified single nucleotide polymorphism (SNP) of HMGA1 was significantly associated with fat deposition traits in the pig. In this study, we identified 3,935 bp nucleotide sequences from exon 5 to exon 8 of the bovine HMGA1 gene and its mRNA expression was observed by quantitative real-time PCR. Six single nucleotide polymorphisms in the bovine HMGA1 gene were detected and the allele frequencies of these SNPs were investigated using the PCR-RFLP method in nine cattle breeds including Limousin, Simmental, Brown Swiss, Hereford, Angus, Charolais, Hanwoo, Brahman and Red Chittagong cattle. The map location showed that the bovine HMGA1 gene was also closely located with a previously identified meat quality QTL region indicating this gene is the most likely positional candidate for meat quality traits in cattle.

• 한국측량학회지
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• 제14권1호
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• pp.9-16
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• 1996

본 연구에서는 상호표정과 사진기준점측량을 위한 새로운 매칭방법을 제안하였다. 이 방법은 수작업에 의한 점이사와 관측을 대신하여 디지탈 항공사진에서 연결점과 접합점으로 사용할 수 있는 공액점들을 매칭한다. 제안된 매칭방법에는 다음과 같은 세 가지의 독특한 단계가 포함되어 있다. 첫째 단계는 interest point를 찾고 이들을 매칭후보점으로 사용한다. 둘째 단계는 중복찰영된 서상에서 표준상관계수법에 의해 양방향으로(왼쪽에서 오른쪽으로 오른쪽에서 왼쪽으로) 매칭을 실시한다. 세째 단계는 양매칭방향에서 일치성이 있는 쌍들을 선택한다. 제안된 매칭방법에 기초한 컴퓨터 프로그램을 개발하고, 전면지상기준점측량을 실시한 디지탈 항공사진을 이용하여 제안된 방법을 사용하였을 때의 신뢰성과 위치정확도를 조사하기 위한 시험을 하였다. 시험 결과 제안된 매칭방법은 다른 매칭방법보다 불량하게 매칭된 쌍들을 제거하는 데 더 효과적이었으며 , 따라서 이 방법을 상호표정 및 사진기준점측량에 사용할 경우 매우 매우 신뢰성이 높다.

• 한국산학기술학회논문지
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• 제17권11호
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• pp.403-409
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• 2016

최근 들어, 나체 사진이나 그림과 같은 유해한 영상 콘텐츠가 쉽게 유통 및 보급되고 있는 실정이어서 이런 유해 영상 콘텐츠를 효과적으로 검출하고 필터링하기 위한 연구 방법들이 지속적으로 소개되고 있다. 따라서 본 논문에서는 입력되는 칼라 영상으로부터 영상의 유해성을 나타내는 요소인 사람의 배꼽 영역을 하르-라이크(Haar-like) 특징과 에이다부스트(Adaboost) 알고리즘을 이용하여 강인하게 검출하는 새로운 접근 방법을 제안한다. 본 논문에서 제안된 방법에서는 먼저 입력 영상으로부터 색상 정보를 이용하여 사람의 유두 영역을 검출하고, 검출된 유두 영역과의 위치 정보를 사용하여 배꼽의 후보 영역을 검출한다. 그런 다음, 하르-라이크 특징과 에이다부스트 알고리즘을 이용한 필터링을 통해 실제 배꼽 영역들만을 검출한다. 실험 결과에서는 제안된 방법이 입력되는 칼라 영상으로부터 배꼽 영역을 기존의 방법보다 1.6% 더 정확하게 추출한다는 것을 보여준다. 본 논문에서 제안된 배꼽 영역 검출 알고리즘은 2 차원이나 3 차원의 유해 콘텐츠 검출 및 필터링과 관련된 여러 가지 응용 분야에서 매우 효과적으로 이용될 것으로 기대된다.

• International Journal of Oral Biology
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• 제39권4호
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• pp.169-176
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• 2014

A positional scanning synthetic peptide combinatorial library (PS-SCL) was screened in order to identify antimicrobial peptides against the cariogenic oral bacteria, Streptococcus mutans. Activity against Streptococcus gordonii and Aggregatibacter actinomycetemcomitans was also examined. The library was comprised of six sub-libraries with the format $O_{(1-6)}XXXXX-NH_2$, where O represents one of 19 amino acids (excluding cysteine) and X represents equimolar mixture of these. Each sub-library was tested for antimicrobial activity against S. mutans and evaluated for antimicrobial activity against S. gordonii and A. actinomycetemcomitans. The effect of peptides was observed using transmission electron microscopy (TEM). Two semi-mixture peptides, RXXXXN-$NH_2$ (pep-1) and WXXXXN-$NH_2$ (pep-2), and one positioned peptide, RRRWRN-$NH_2$ (pep-3), were identified. Pep-1 and pep-2 showed significant antimicrobial activity against Gram positive bacteria (S. mutans and S. gordonii), but not against Gram negative bacteria (A. actinomycetemcomitans). However, pep-3 showed very low antimicrobial activity against all three bacteria. Pep-3 did not form an amphiphilic ${\alpha}$-helix, which is a required structure for most antimicrobial peptides. Pep-1 and pep-2 were able to disrupt the membrane of S. mutans. Small libraries of biochemically-constrained peptides can be used to generate antimicrobial peptides against S. mutans and other oral microbes. Peptides derived from such libraries may be candidate antimicrobial agents for the treatment of oral microorganisms.

• 한국수정란이식학회지
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• 제30권3호
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• pp.137-142
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• 2015

Cryopreservation of boar semen is continually researched in reproductive technologies and genetic resource banking in breed conservation. For evaluating the boar semen quality, sperm motility (MOT) is an important parameter because the movement of spermatozoa indicates active metabolism, membrane integrity and fertilizing capacity. Various researches have been trying to improve the quality of semen post-thawed in boar. Recently, polymorphism (g.158T>C) of phospholipase C zeta (PLCz) gene reported to be significant association with MOT. This study was conducted to evaluate the PLCz gene as a positional controlling for motility and kinematic characteristics of post-thawed boar semen. To results, The g.158 T>C SNP of PLCz was significantly associated with frozen semen motility and kinematic characteristics. g.158 T>C SNP was high significantly associated with MOT, VCL, VSL and VAP (p<0.0001, p=0.0002, p<0.0001 and p<0.0001, respectively). Therefore, we suggest that the intron region of the porcine PLCz, may be used as a molecular marker for Duroc boar post-thawed semen quality, although its functional effect was not defined yet. Whether the association is due to the candidate gene or not require further verification. Thus, it will be of interest to continue association studies in the regions surrounding those genes.