• Biomolecules & Therapeutics
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• 제27권2호
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• pp.210-215
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• 2019

Colorectal cancer is one of the leading causes of cancer related death due to a poor prognosis. In this study, we investigated the effect of Gomisin G on colon cancer growth and examined the underlying mechanism of action. We found that Gomisin G significantly suppressed the viability and colony formation of LoVo cells. Gomisin G reduced the phosphorylation level of AKT implying that Gomisin G suppressed the PI3K-AKT signaling pathway. Gomisin G also induced apoptosis shown by Annexin V staining and an increased level of cleaved poly-ADP ribose polymerase (PARP) and Caspase-3 proteins. Furthermore, Gomisin G remarkably triggered the accumulation of cells at the sub-G1 phase which represents apoptotic cells. In addition, the level of cyclin D1 and phosphorylated retinoblastoma tumor suppressor protein (Rb) was also reduced by the treatment with Gomisin G thus curtailing cell cycle progression. These findings show the suppressive effect of Gomisin G by inhibiting proliferation and inducing apoptosis in LoVo cells. Taken together, these results suggest Gomisin G could be developed as a potential therapeutic compound against colon cancer.

• Clinics in Shoulder and Elbow
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• 제22권2호
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• pp.100-105
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• 2019

Suture anchors are commonly used in shoulder surgeries, especially for rotator cuff tears. Peri-anchor cyst formation, however, is sometimes detected on follow-up radiologic image after surgery. The purpose of this report is to discuss the case of a patient who presented with regression of extensive peri-anchor cyst on postoperative 4-year follow-up magnetic resonance imaging and had good clinical outcome despite peri-anchor cyst formation after arthroscopic rotator cuff repair.

• Bulletin of the Korean Chemical Society
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• 제17권3호
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• pp.257-262
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• 1996

p-(2-Vinyloxyethoxy)benzylidenemalononitrile 2 and methyl p-(2-vinyloxyethoxy)benzylidenecyanoacetate 3 was prepared by the condensation of p-(2-vinyloxyethoxy)benzaldehyde 1 with malononitrile or methyl cyanoacetate, respectively. Vinyl ether monomers 2 and 3 polymerized quantitatively with radical initiators in γ-butyrolactone solution at 65 ℃. The trisubstituted terminal double bond participated in the vinyl polymerization and radical polymerization of 2 and 3 led to swelling polymers 4 and 5 that were not soluble in common solvents due to cross-linking. Under the same polymerization conditions ethylvinyl ether polymerized well with model compounds of p-methoxybenzylidenemalononitrile 6 and methyl p-methoxybenzylidenecyanoacetate 7, respectively, to give 1:1 alternating copolymers 8 and 9 in high yields. Polymers 4 and 5 showed a thermal stability up to 300 ℃ without any characteristic Tg peaks in DSC thermograms. Alternating copolymers 8 and 9 were soluble in common solvents such as acetone and DMSO, and the inherent viscosities of the polymers were in the range of 0.36-0.74 dL/g. Films cast from acetone solution were cloudy and tough and Tg values obtained from DSC thermograms were in the range of 59-60 ℃.

• Bulletin of the Korean Chemical Society
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• 제18권3호
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• pp.318-323
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• 1997

ο-(2-Vinyloxyethoxy)benzylidenemalononitrile (3a) and methyl ο-(2-vinyloxyethoxy)-benzylidenecyanoacetate (3b), m-(2-vinyloxyethoxy)benzylidenemalononitrile (4a), and methyl m-(2-vinyloxyethoxy)benzylidenecyanoacetate (4b) were prepared by the condensation of ο-(2-vinyloxyethoxy)benzaldehyde (1) and m-(2-vinyloxyethoxy)benzaldehyde (2) with malononitrile or methyl cyanoacetate, respectively. Bifunctional vinyl ether monomers 3a and 3b polymerized quantitatively with radical initiators in γ-butyrolactone solution at 65 ℃, while meta-isomers 4a and 4b gave lower yields of polymers under the same conditions. The polymers 5-6 obtained from the monomers 3-4 were insoluble in common solvents due to cross-linking. Under the same polymerization conditions ethyl vinyl ether polymerized well with model compounds ο-methoxybenzylidenemalononitrile 7a, methyl ο-methoxybenzylidenecyanoacetate 7b, m-methoxybenzylidenemalononitrile 8a, and methyl m-methoxybenzylidenecyanoacetate 8b, respectively, to give 1:1 alternating copolymers 9-10 in high yields. Cross-linked polymers 5-6 showed a thermal stability up to 300 ℃, and showed a double phase degradation pattern in their TGA thermograms. Polymers 5-6 showed broad endothermic bands around 75-110 ℃ without any characteristic Tg peaks in DSC thermograms. Alternating copolymers 9-10, except copolymer 9b were soluble in common organic solvents. The inherent viscosities of polymer 9-10 were in the range of 0.35-0.62 dL/g. Polymer films cast from acetone solution were cloudy and tough and Tg values obtained from DSC thermograms were in the range of 118-165 ℃.

• 한국정보통신학회논문지
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• 제24권2호
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• pp.325-328
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• 2020

This paper demonstrated a voltage-controlled oscillator (VCO) using cost-effective (1-poly 6-metal) mixed signal standard CMOS process. To have the high-quality factor inductor in LC resonator with thin metal thickness, patterned-ground shields (PGS) was adopted under the spiral to effectively reduce the ac current of low resistive Si substrate. And, because of thin top-metal compared with that of RF option (2 ㎛), we make electrically connect between the top metal (M6) and the next metal (M5) by great number of via array along the metal traces. The circuit operated from 2.48 GHz to 2.62 GHz tuned by accumulation-mode varactor device. And the measured phase noise of LC VCO has -123.7 dBc/Hz at 1MHz offset at 2.62 GHz and the dc-power consumption shows 2.07 mW with 1.8V supply voltage, respectively.

• 한국입자에어로졸학회지
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• 제18권4호
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• pp.109-127
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• 2022

Recently, low-cost particulate matter (PM) sensors have been widely used in monitoring mass concentration. Maintaining the accuracy of the sensors is important and requires rigorous performance evaluation and calibration. In this study, two commercial low-cost PM sensors(LCS), Plantower PMS3003 and Plantower PMS7003, were evaluated in the laboratory and field with a reference-grade PM monitor (GRIMM 11-D). Laboratory evaluation was conducted with single/mixed particles of PSL (Poly Styrene Latex) in an acrylic chamber at 20℃ and relative humidity of 20%. Field evaluation was conducted inside a building of Yonsei University (Shinchon) from February 12 to March 31, 2022. In both evaluations, LCS measured values became different from reference measured values when the relative humidity was high or the outdoor air PM10/PM2.5 ratio was high. Based on the field evaluation, the LCS measured values were corrected through four different regression analysis models. As a result, the multivariate polynomial regression analysis model showed highest matching with the reference PM monitor (PM2.5 >0.9, PM10 >0.85). In this model, the PM10/PM2.5 ratio and relative humidity were chosen as independent variables.

• 미생물학회지
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• 제31권3호
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• pp.179-183
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• 1993

A restricted facultatively methanol-oxidizing bacterium, Methylovorus sp. strain SS1, was isolate dfrom soil samples from Kuala Lumpur, Malaysia, through methanol-enrichment culture technique. The isolate was nonmotile Gram-negative rod and did not have complex internal membrane system. The colonies were small, pale-yellow, and raised convex with entire margin. The cell did not produce any spores and capsular materials. The cell was obligately aerobic and exhibited catalase, but no oxidase, activity. Plasmid, carotenoid pigment, and poly-.betha.-hydroxybutyric acid were not found. The guanine plus cytosine content of the DNA was 55%. The isolate was found to grow only on methanol methylamine, or glucose. Growth factors were not required. Cells growing on methanol was found to produce extracellular polysaccharides containing glucose, lactose, and fructose. Growth was optimal (t$_{d}$= 1.7) with 0.5%(v/v) methanol at 40.deg.C and pH 6.5. No Growth was observed at over 60.deg.C. Cell-free extracts of the methanol grown cells exhibited the phenazine methosulfate-linked methanol dehydrogenase activity Methanol was found to be assimilate dthrough the ribulose monophosphate pathway.y.

• 생명과학회지
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• 제23권3호
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• pp.389-398
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• 2013

Platycodin D는 도라지(Platycodon grandiflorum)의 뿌리인 길경(Platycodi Radix)에 함유된 은 triterpene saponin의 일종으로 다양한 약리효능이 있는 것으로 알려져 있다. 본 연구에서는 다양한 암세포 중 platycodin D의 항암활성 감수성이 가장 높았던 인체 혈구암 U937 세포를 대상으로 platycodin D 처리에 따른 apoptosis 유발기전에 대하여 조사하였다. 본 연구의 결과에 의하면 U937 세포에서 platycodin D 처리에 의한 증식억제는 apoptosis 유발과 밀접한 연관이 있음을 알 수 있었다. 이러한 platycodin D에 의한 apoptosis 유발은 pro-apoptotic Bax의 발현 증가와 연관된 미토콘드리아 막 전위의 소실 및 caspase-3의 활성화와 기질단백질들의 분해에 의한 것임을 알 수 있었으며, IAP family 인자들의 발현 감소가 caspase의 활성 증가에도 영향을 미친 것으로 추정된다. 또한 z-DEVD-fmk 선처리에 의하여 platycodin D에 의하여 유발된 apoptosis가 유의적으로 억제되었기에, platycodin D 처리에 의하여 유발되는 apoptosis는 미토콘드리아 경로에 의하여 조절되며, caspase-3의 활성화가 핵심적인 역할을 한 것으로 생각된다.

• 한국해양환경ㆍ에너지학회지
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• 제13권2호
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• pp.99-104
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• 2010

본 연구는 선박에서 발생되는 오폐수를 시중에서 많이 사용되고 있는 Poly Propylene를 이용한 고정식 메디아법의 처리효과를 검증하고, MLTM 및 IMO의 MEPC에서 규정하는 2010년 1월 1일 이후 선박에 설치되는 선박분뇨처리 장치의 처리수의 처리 기준에 적합하고 현장에 적용 가능한 선박분뇨처리장치를 Pilot Scale을 통해 개발 가능성을 고찰하였다. 배출수의 pH 기하평균은 7.68이고, $BOD_5$ 기하평균은 7.28 mg/l, $COD_{cr}$ 기하평균은 48.39 mg/l, TSS 기하평균은 18.00 mg/l, 잔류염소 기하평균은 0.19 mg/l, 분원성 대장균 기하평균은 1 cells/100 ml로 측정되었다. 각 항목별 처리효율은 $BOD_5$ 97.4%, $COD_{cr}$ 96.4%, TSS 97.6%로 높은 처리효율을 나타났으며, 개발한 선박분뇨처리 장치는 국토해양부 및 MEPC의 해양오염방지설비 성능시험 규정에 적합한 제품으로 판정되었다.

• Journal of Korean Dental Science
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• 제12권1호
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• pp.13-19
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• 2019

Purpose: The aim of this study was to compare the flexural strength of provisional fixed dental prostheses which was three-dimensional (3D) printed by several build directions. Materials and Methods: A metal jig with two abutment teeth and pontic space in the middle was fabricated. This jig was scanned with a desktop scanner and provisional restoration was designed on dental computer-aided design program. On the preprocessing software, the build angles of the restorations were arranged at $0^{\circ}$, $30^{\circ}$, $45^{\circ}$, $60^{\circ}$, and $90^{\circ}$ and support was added and resultant structure was sliced to a thickness of $100{\mu}m$. Processed restorations were printed with digital light processing type 3D printer using poly methyl meta acrylate-based resin. After washing and post-curing, compressive loading was applied at a speed of 1 mm/min on a metal jig fixed to a universal testing machine. The maximum pressure at which fracture occurred was measured. For the statistical analysis, build direction was set as the independent variable and fracture strength as the dependent variable. One-way analysis of variance and Tukey's post hoc analysis was conducted to compare fracture strength among groups (${\alpha}=0.05$). Result: The mean flexural strength of provisional restoration 3D printed with the build direction of $0^{\circ}$ was $1,053{\pm}168N$; it was $1,183{\pm}188N$ at $30^{\circ}$, $1,178{\pm}81N$ at $45^{\circ}$, $1,166{\pm}133N$ at $60^{\circ}$, and $949{\pm}170N$ at $90^{\circ}$. The group with a build direction of $90^{\circ}$ showed significantly lower flexural strength than other groups (P<0.05). The flexural strength was significantly higher when the build direction was $30^{\circ}$ than when it was $90^{\circ}$ (P<0.01). Conclusion: Among the build directions $0^{\circ}$, $30^{\circ}$, $45^{\circ}$, $60^{\circ}$, and $90^{\circ}$ set for 3D printing of fixed dental prosthesis, an orientation of $30^{\circ}$ is recommended as an effective build direction for 3D printing.