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http://dx.doi.org/10.4062/biomolther.2018.054

Gomisin G Suppresses the Growth of Colon Cancer Cells by Attenuation of AKT Phosphorylation and Arrest of Cell Cycle Progression  

Maharjan, Sony (Center for Medical Science Research, College of Medicine, Hallym University)
Park, Byoung Kwon (Center for Medical Science Research, College of Medicine, Hallym University)
Lee, Su In (Center for Medical Science Research, College of Medicine, Hallym University)
Lim, Yoongho (Division of Bioscience and Biotechnology, BMIC, Konkuk University)
Lee, Keunwook (Department of Biomedical Science, College of Natural Science, Hallym University)
Lee, Younghee (Department of Biochemistry, College of Natural Sciences, Chungbuk National University)
Kwon, Hyung-Joo (Center for Medical Science Research, College of Medicine, Hallym University)
Publication Information
Biomolecules & Therapeutics / v.27, no.2, 2019 , pp. 210-215 More about this Journal
Abstract
Colorectal cancer is one of the leading causes of cancer related death due to a poor prognosis. In this study, we investigated the effect of Gomisin G on colon cancer growth and examined the underlying mechanism of action. We found that Gomisin G significantly suppressed the viability and colony formation of LoVo cells. Gomisin G reduced the phosphorylation level of AKT implying that Gomisin G suppressed the PI3K-AKT signaling pathway. Gomisin G also induced apoptosis shown by Annexin V staining and an increased level of cleaved poly-ADP ribose polymerase (PARP) and Caspase-3 proteins. Furthermore, Gomisin G remarkably triggered the accumulation of cells at the sub-G1 phase which represents apoptotic cells. In addition, the level of cyclin D1 and phosphorylated retinoblastoma tumor suppressor protein (Rb) was also reduced by the treatment with Gomisin G thus curtailing cell cycle progression. These findings show the suppressive effect of Gomisin G by inhibiting proliferation and inducing apoptosis in LoVo cells. Taken together, these results suggest Gomisin G could be developed as a potential therapeutic compound against colon cancer.
Keywords
Gomisin G; Colon cancer; AKT; Apoptosis; PARP; Cell cycle;
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