• Title/Summary/Keyword: PnP

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Development of Prevotella nigrescens ATCC $33563^T$-Specific PCR Primers (Prevotella nigrescens ATCC $33563^T$ 균주-특이 중합효소연쇄반응 프라이머 개발)

  • Song, Soo-Keun;Yoo, So-Young;Kim, Mi-Kwang;Kim, Hwa-Sook;Lim, Sun-A;Kim, Do-Kyung;Park, Jae-Yoon;Kook, Joong-Ki
    • Korean Journal of Microbiology
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    • v.44 no.3
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    • pp.212-220
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    • 2008
  • A Pn10 DNA probe was introduced as a Prevotella nigrescens ATCC $33563^T$-specific DNA probe. In that study, the specificity of the Pn10 was tested with only type or reference strains of 5 oral bacterial species. The purpose of this study is to evaluate the specificity of the Pn10 using the wild type strains of P. nigrescens and is to develop the P. nigrescens ATCC $33563^T$-specific PCR primers based on the nucleotide sequence of the Pn10. The specificity of the Pn10 DNA probe was determined by Southern blot analysis. The nucleotide sequence of Pn10 DNA probes was determined by chain termination method. The PCR primers were designed based on the nucleotide sequence of cloned DNA fragment. The data showed that Pn10 DNA probe were hybridized with the genomic DNAs from P. nigrescens ATCC $33563^T$ and KB6. The Pn10 homologous region, KB6-Pn10, of P. nigrescens KB6 was cloned by PCR and sequenced. The Pn10 and KB6-Pn10 DNA fragments were consisted of 1,875 bp and 1,873 bp, respectively. The percent identity of the two was 98.8% and the divergence of them was 0.6%. The two primer sets (Pn10-F-AC/ Pn10-R-AC and Pn10-F-A/ Pn10-R-A), designed base on the nucleotide sequences of Pn10 DNA probe, were specific to the P. nigrescens ATCC $33563^T$. The two PCR primer sets could detect as little as 4 pg of genomic DNA of P. nigrescens ATCC $33563^T$. These results indicate that the two PCR primer sets have proven useful for the identification of P. nigrescens ATCC $33563^T$, especially with regard to the maintenance of the strain.

A Study on the PN code Acquisition for DS/CDMA System over Phas-Error (위상에러를 고려한 DS/CDMA시스템의 PN 부호 획득에 관한 연구)

  • 정남모
    • Journal of the Korea Society of Computer and Information
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    • v.7 no.3
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    • pp.128-134
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    • 2002
  • In this paper, the performance on the PN code acquisition of DS/CDMA system was analyzed using the Nakagami-m probability density function considered fading environment. The equations on detection probability, $P_D$ and false alarm probability, $P_{FA}$, decision variables affecting the PN code acquisition time were derived and proved using simulation in order to analyze the performance. In conclusion, It was necessary increasing the gain of PLL for correcting phase errors and improving the acquisition performance of PN code in apply to the rake receiver.

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A PN-code Acquisition method Using Array Antenna Systems for CDMA2000 1x (CDMA2000 1x용 배열 안테나 시스템에서 PN 동기 획득 방법)

  • Jo, Hee-Nam;Yun, Yu-Suk;Choi, Seung-Won
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.42 no.8 s.338
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    • pp.33-40
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    • 2005
  • This paper presents a structure of the searcher using a diversity in array antenna systems operating in the cdma2000 1x signal environments. The new technique exploits the fact that the In-phase and quadrature components of interferers can respectively be viewed as an independent gaussian noise at each antnna element in most practical cdma signal environments. The proposed PN acquisition scheme is a singles-dwell PN acquisition system consisting of two stages, that is, the searching stage and the verification stage. The searching stage independently correlates the receiver multiple signals with PN generator of each antenna element for obtaining the synchronous energy at the entire region. Then, the searching results of each antenna element are non-coherently combinind. The verification stage compares the searching energy with the optimal threshold, which is predesigned in the lock detector, and decides whether the acquisition is successful or fail. In this paper, we analyzed the effect of tile diversity order to determine the mean acquisition time. In general, it is known that the mean acquisition time significantly decrease as the number of antenna elements increases. But, as the diversity order goes up, the enhancement of the performance is saturated. Therefore, to decrease the mean acquisition time of the searcher, we must design the optimal array antenna systems by considering the operating SNR range of the receiver, the probability of detection $P_D$ and that of false alarm $P_{FA}$ . The Performance of the proposed PN acquisition scheme is analyzed in frequency selective Rayleigh fading channels. In this paper, the effect of the number of antenna elements on PN acquisition scheme is shown according to the probability of detection $P_D$ and that of false alarm $P_{FA}$.

A Study on the PN code Acquisition for DS/CDMA System over Phase-Error (위상 오류를 고려한 DS/CDMA 시스템의 PN 부호 획득에 관한 연구)

  • 정남모;강찬석;장문기
    • Journal of the Institute of Electronics Engineers of Korea TE
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    • v.39 no.4
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    • pp.403-408
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    • 2002
  • In this paper, the performance on the PN code acquisition of DS/CDMA system was analyzed using the Nakagami-m probability density function considered fading environment. The equations on detection probability, $P_D$ and false alarm probability, ($P_{FA}$, decision variables affecting the PN code acquisition time were derived and proved using simulation in order to analyze the performance. In conclusion, It was necessary increasing the gain of PLL for correcting phase errors and improving the acquisition performance of PN code in apply to the rake receiver.

Genetic Algorithm Calibration Method and PnP Platform for Multimodal Sensor Systems (멀티모달 센서 시스템용 유전자 알고리즘 보정기 및 PnP 플랫폼)

  • Lee, Jea Hack;Kim, Byung-Soo;Park, Hyun-Moon;Kim, Dong-Sun;Kwon, Jin-San
    • The Journal of the Korea institute of electronic communication sciences
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    • v.14 no.1
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    • pp.69-80
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    • 2019
  • This paper proposes a multimodal sensor platform which supports plug and play (PnP) technology. PnP technology automatically recognizes a connected sensor module and an application program easily controls a sensor. To verify a multimodal platform for PnP technology, we build up a firmware and have the experiment on a sensor system. When a sensor module is connected to the platform, a firmware recognizes the sensor module and reads sensor data. As a result, it provides PnP technology to simply plug sensors without any software configuration. Measured sensor raw data suffer from various distortions such as gain, offset, and non-linearity errors. Therefore, we introduce a polynomial calculation to compensate for sensor distortions. To find the optimal coefficients for sensor calibration, we apply a genetic algorithm which reduces the calibration time. It achieves reasonable performance using only a few data points with reducing 97% error in the worst case. The platform supports various protocols for multimodal sensors, i.e., UART, I2C, I2S, SPI, and GPIO.

Detection of Prevotella intermedia and Prevotella nigrescens using Pn17 and Pn34 DNA Probes

  • Park, Chan-Ho;Kim, Pan-Soon;Kim, Hwa-Sook;Min, Jeong-Bum;Hwang, Ho-Keel;Jang, Hyun-Sun;Cho, Ki-Woon;Baek, Dong-Heon;Kook, Joong-Ki
    • International Journal of Oral Biology
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    • v.35 no.1
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    • pp.13-19
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    • 2010
  • The DNA probes Pn17 and Pn34 were evaluated for their ability to specifically detect clinical strains of P. intermedia and P. nigrescens from a Korean population by dot blot hybridization. These probes were sequenced by extension termination and their specificity was determined by Southern blot analysis. The results revealed that the Pn17 sequence (2,517 bp) partially encodes an RNA polymerase beta subunit (rpoB) and that Pn34 (1,918 bp) partially encodes both rpoB (1-169 nts) and the RNA polymerase beta subunit (rpoB'; 695-1918 nts). These probes hybridized with both HindIII- and PstI-digested genomic DNAs from the strains of P. intermedia and P. nigrescens used in this study. Interestingly, each of the hybrid bands generated from the HindIII-digested genomic DNAs of the two bacterial species could be used to distinguish between them via restriction fragment length polymorphism. These results thus indicate that Pn17 and Pn34 can simultaneously detect P. intermedia and P. nigrescens.

Effect of Bioenzyme Application on the Growth of Lettuce(Lactuca Sativa L.) (Bioenzyme 처리가 상추의 수량에 미치는 영향)

  • Kang, Sang-Jae;Park, Woo-Churl
    • Current Research on Agriculture and Life Sciences
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    • v.15
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    • pp.101-107
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    • 1997
  • Bioenzyme was made up of two predominant strain, which were nomenclated PN-1 and PN-2, respectively. PN-1 possessed spore forming ability, motility, gram positive, catalase activity, but didn't have oxidase activity, urease activity, nitrate reduction and indole forming ability. Taxonomical characteristics of the PN-2 were similar to PN-1 strain, but colony color of PN-2 was yellow-white and nitrate reduction ability was positive. Predominant strains were ascertained by physiologically and morphologically. PN-1 and PN-2 were identified Bacillus lichenifonnis and Bacillus thuringinensis, respectively. Yield of lettuce significantly increased in first and second bioenzyme treatment. Fresh weight, length of shoot, width of leaves significantly increased in N,P,K and N,P,K followed by Bioenzyme application.

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ON THE DIOPHANTINE EQUATION (5pn2 - 1)x + (p(p - 5)n2 + 1)y = (pn)z

  • Kizildere, Elif;Soydan, Gokhan
    • Honam Mathematical Journal
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    • v.42 no.1
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    • pp.139-150
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    • 2020
  • Let p be a prime number with p > 3, p ≡ 3 (mod 4) and let n be a positive integer. In this paper, we prove that the Diophantine equation (5pn2 - 1)x + (p(p - 5)n2 + 1)y = (pn)z has only the positive integer solution (x, y, z) = (1, 1, 2) where pn ≡ ±1 (mod 5). As an another result, we show that the Diophantine equation (35n2 - 1)x + (14n2 + 1)y = (7n)z has only the positive integer solution (x, y, z) = (1, 1, 2) where n ≡ ±3 (mod 5) or 5 | n. On the proofs, we use the properties of Jacobi symbol and Baker's method.

Cryopreservation of Human Multi-Pronuclear (PN) Zygote by Ultra-Rapid Freezing (인간 다-전핵기 (>2PN) 수정란의 초급속 동결에 관한 연구)

  • Kim, E.Y.;Yi, B.K.;Nam, H.K.;Lee, K.S.;Yoon, S.H.;Park, S.P.;Chung, K.S.;Lim, J.H.
    • Clinical and Experimental Reproductive Medicine
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    • v.25 no.2
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    • pp.129-134
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    • 1998
  • The objective of this study was to test whether the developmental capacity of human multi-pronuclear (PN) zygotes after ultra-rapid freezing using EM grid can be maintained. For this experiment, multi-PN zygotes which produced in human IVF program were used as an alternatives of normal 2PN zygotes, and they were separated into 3PN or $\geq4PN$ zygotes to compare their in vitro development and cryoinjury according to PN number. As freezing solution, EFS30 which consisted of 30% ethylene glycol, 18% bcoll, 0.5 M sucrose and 10% FBS added D-PBS was used. The result obtained in this experiment was summarized as follows; When the multi..PN zygotes were ultrarapidly frozen and thawed, the high mean percentages (85.5%) were survived. Also when the cleavage rates between control and freezing group were compared with PN number, there were not significantly different in each group (3PN; 81.3% & 85.4% and $\geq4PN$; 90.0% & 95.7%). When the in vitro development rates after thawing were examined, freezing 3PN group (22.0%) was not differed to control 3PN group (38.5%), although the development result of freezing $\geq4PN$ group (45%) was significantly lower than that of control $\geq4PN$ group (44.4%) (p<0.05). These results demonstrate that developmental capacity of human zygote can be obtained by ultra-rapid freezing method using EM grid and EFS30.

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Study on the pn Junction Device Using the POCl3 Precursor (POCl3를 사용한 pn접합 소자에 관한 연구)

  • Oh, Teresa
    • Journal of the Korean Vacuum Society
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    • v.19 no.5
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    • pp.391-396
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    • 2010
  • The pn junction for solar cell was prepared on p-type Si wafer by the furnace using the $POCl_3$ and oxygen mixed precursor to research the characteristic of interface at pn junction. The sheet resistance was decreased in accordance with the increasing the diffusion process time for n-type doping on p-type Si wafer. The electron affinity at the interface in the pn junction was decreased with increasing the amount of n-type doping and the sheet resistance also decreased. Consequently, the drift current due to the generation of EHP increased because of low potential barrier. The efficiency and fill factor were increased at the solar cell with increasing the diffusion process time.