International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Detection of Prevotella intermedia and Prevotella nigrescens using Pn17 and Pn34 DNA Probes

  • Park, Chan-Ho (Department of Conservative Dentistry, School of Dentistry, Chosun University) ;
  • Kim, Pan-Soon (Department of Periodontology, School of Dentistry, Chosun University) ;
  • Kim, Hwa-Sook (Department of Dental Hygiene, Chunnam Techno College, Gokseong County) ;
  • Min, Jeong-Bum (Department of Conservative Dentistry, School of Dentistry, Chosun University) ;
  • Hwang, Ho-Keel (Department of Conservative Dentistry, School of Dentistry, Chosun University) ;
  • Jang, Hyun-Sun (Department of Periodontology, School of Dentistry, Chosun University) ;
  • Cho, Ki-Woon (Department of Oral Biochemistry, School of Dentistry, Chosun University) ;
  • Baek, Dong-Heon (Department of Oral Microbiology & Immunology, College of Dentistry, Dankook University) ;
  • Kook, Joong-Ki (Department of Oral Biochemistry, School of Dentistry, Chosun University)
  • Received : 2009.12.23
  • Accepted : 2010.01.22
  • Published : 2010.03.31
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Abstract

The DNA probes Pn17 and Pn34 were evaluated for their ability to specifically detect clinical strains of P. intermedia and P. nigrescens from a Korean population by dot blot hybridization. These probes were sequenced by extension termination and their specificity was determined by Southern blot analysis. The results revealed that the Pn17 sequence (2,517 bp) partially encodes an RNA polymerase beta subunit (rpoB) and that Pn34 (1,918 bp) partially encodes both rpoB (1-169 nts) and the RNA polymerase beta subunit (rpoB'; 695-1918 nts). These probes hybridized with both HindIII- and PstI-digested genomic DNAs from the strains of P. intermedia and P. nigrescens used in this study. Interestingly, each of the hybrid bands generated from the HindIII-digested genomic DNAs of the two bacterial species could be used to distinguish between them via restriction fragment length polymorphism. These results thus indicate that Pn17 and Pn34 can simultaneously detect P. intermedia and P. nigrescens.

Keywords

References

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