• Title/Summary/Keyword: Pleurotus cornucopiae

Search Result 41, Processing Time 0.026 seconds

Cultural characteristics and phylogenetic relationships based on RFLP analysis in Pleurotus species

  • Choi, Sun-Gyu;Jang, Kab-Yeul;Kim, Gyu-Hyun;Kong, Won-Sik;Jo, Jae-Sun;Kim, Hae-Yeong;Yoo, Young-Bok
    • Journal of Mushroom
    • /
    • v.12 no.3
    • /
    • pp.145-153
    • /
    • 2014
  • Pleurotus has increased rapidly production and consumption because of highly nutritional value, natural healthy food and so on. The basic studies for Pleurotus need for development of mushroom industry. This study was to investigate the cultural characteristics among 15 strains of 6 species and to analyze their phylogenetic relationships. The cultural characteristics were investigated by mycelial growth activity at different media, temperature and pH. The optimum media for mycelial growth were YM and MCM in most species. The optimum temperature for mycelial growth were $25^{\circ}C$ and $30^{\circ}C$. The optimum pH for mycelial growth were widly range from pH 5.1 to 7.4. Through the RFLP (restriction fragment length polymorphism) of IGS (intergenic spacer) I region in ribosomal DNA, it was analyzed phylogeny of interspecies and intraspecies. Each species was discriminated well as isolates within each species formed clade to be distinguished other species. P. florida was highly similar to P. floridanus, and P. flabellatus was P. cornucopiae. P. fuscus var. ferulae was highly similar to P. eryngii but discriminated different species in analysis of RFLP of IGS I region and showed different characteristics in mycelial culture. RFLP of IGS I region was useful of studying phylogenetic relationships of species and population.

Characterization of a new commercial strain 'Gumbit' in Pleurotus cornucopiae var. citrinopileatus (노랑느타리 품종 '금빛'의 특성)

  • Yoo, Young-Bok;Kong, Won-Sik;Jang, Kab-Yeul;Kim, In-Yeup;Oh, Se-Jong;Jhune, Chang-Sung
    • Journal of Mushroom
    • /
    • v.4 no.3
    • /
    • pp.83-87
    • /
    • 2006
  • A new commercial strain "Gumbit" of golden oyster mushroom was developed by hyphal anastomosis. It was improved with hybridization between monokaryotic strain derived from ASI 2295 and ASI 2703. The optimum temperature of mycelial growth and fruiting body development were $25{\sim}30^{\circ}C$ and $19{\sim}24^{\circ}C$, respectively. The pileus was golden to brilliant yellow color. Commercial strain "Gumbit" was not as prolific as the more commonly cultivated Pleurotus ostreatus in the conversion of substrate mass to mushrooms. However, cultivator can save money for mushroom growing on high-temperature season as like summer in Korea. Since picking individual mushrooms is tedious and often damages the fragile fruiting bodies compared with other species of oyster mushrooms.

  • PDF

Optimization of Extraction Conditions of Pleurotus cornucopiae by Response Surface Methodology (반응표면분석에 의한 노랑느타리버섯 추출물의 추출조건 최적화)

  • Lee, Hye-Jin;Do, Jeong-Ryong;Chung, Min-Yu;Kim, Hyun-Ku
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.43 no.10
    • /
    • pp.1565-1570
    • /
    • 2014
  • Response surface methodology (RSM) was used to optimize extraction conditions of Pleurotus cornocopiae. Coefficients of determination (R2) for dependent variables ranged from 0.86 at 0.91. Maximum extraction yield was 40.81% under the following conditions: microwave power of 60.08 watts, ethanolic concentration of 12.33%, and extraction time of 6.86 min. Maximum extraction electron donating ability was 35.72% at 44.13 watts, 19.30%, and 4.21 min. Maximum extraction superoxide dismutase (SOD)-like activity was 34.87% at 114.01 watts, 65.88%, and 1.56 min. Maximum extraction total polyphenol content was 31.77 mg/g at 50.52 watts, 23.00% and 2.90 min. Based on the superimposition of four dimensional RSM with respect to extraction yield, electron donating ability, SOD-like activity, and total polyphenol content obtained under various extraction conditions, the optimum ranges of extraction conditions were as follows: microwave power of 71.48~92.84 watts, ethanol concentration of 55.01~71.66%, and extraction time of 3~9 min.

Preparation of fermented vinegars using mushroom mycelial cultures and characterization of their physicochemical properties (버섯 균사체를 이용한 발효 식초 제조 및 이화학적 특성)

  • Kim, Jeong-Han;Baek, Il-Sun;Shin, Bok-Eum;Lee, Yong-Seon
    • Journal of Mushroom
    • /
    • v.17 no.4
    • /
    • pp.230-234
    • /
    • 2019
  • This study was conducted to prepare healthy vinegars using the mycelia of various mushrooms (Cordyceps militaris, Phellinus baumi, Pleurotus cornucopiae, and Ganoderma lucidum). Analyses of the bioactive components of these vinegars revealed that those prepared using mushroom mycelial cultures on fermented brown rice had increased bioactive component levels compared to those cultured on unfermented brown rice. The highest beta-glucan contents (78.7 mg/g) were found in P. baumi cultures and 0.34mg/g of cordycepin was detected in C. militaris cultures. Regarding the chemical properties of these mushroom mycelia vinegars (MMVs), those prepared using C. militaris and P. cornucopiae cultures showed higher acidity and reduced sugar content. Sensory assessments of C. militaris and P. cornucopiae MMVs yielded scores of 7.3 and 6.6, respectively. Analyses of bioactive components revealed that C. militaris MMV contained high levels of polyphenols and 786 mg/L of cordycepin. P. cornucopiae MMV contained the highest amount of beta-glucan (20.9 mg/g).

Interspecific protoplast Fusion and Sexuality in Pleurotus (느타리의 종간(種間) 원형질체(原形質體) 융합(融合) 및 유성(有性))

  • Yoo, Young-Bok;You, Chang-Hyun;Park, Yong-Hwan;Lee, Yeon-Hee;Chang, Kwon-Yawl;Peberdy, John F.
    • The Korean Journal of Mycology
    • /
    • v.15 no.3
    • /
    • pp.135-141
    • /
    • 1987
  • Interspecific heterokaryons were obtained by polyethylene glycol induced fusion of protoplasts from auxotrophic mutants of P. ostreatus+P. cornucopiae, P. ostreatus+P. florida, P. ostreatus+P. sajor-caju, P. florida+P. cornucopiae, P. florida+P. sajor-caju and P. sajor-caju+P. cornucopiae protoplasts. The Fusion products of protoplasts were induced by MCM+benomyl, but segregation of sectors were not identified. Interspecific fusion products of protoplasts between incompatible strains did not form true clamp connections and did not produce fruit bodies. For induction of fertility, interspecific heterokaryons crossed with their parents by hyphal anastomosis.

  • PDF

Comparative analysis of anti-oxidant effects and polyphenol contents of the fruiting bodies in oyster mushrooms (느타리버섯 균주별 항산화효능 및 폴리페놀함량 비교분석)

  • Cho, Jae-Han;Park, Hye-Sung;Han, Jae-Gu;Lee, Gang-Hyo;Sung, Gi-Ho;Jhune, Chang-Sung
    • Journal of Mushroom
    • /
    • v.12 no.4
    • /
    • pp.311-315
    • /
    • 2014
  • Oyster mushroom is one of the popular mushrooms for Korean people and it is thus one of the mushrooms that were mostly cultivated in Korea in addition to winter and king oyster mushrooms. This study was carried out to compare the medicinal effects of fruiting body of Pleurotus ostreatus extracts. We analyzed comparing antioxidant activity and polyphenol contents of fruiting body of Pleurotus ostreatus. Firstly, ASI 2099 showed the highest level in hot water. ASI 2122 showed the highest level in the 70% EtOH and MeOH extraction. Polyphenol contents was measured after extraction with different solvents. polyphenols contents is mostly 8~10 (mg/g) and there is no difference of polyphenols contents depending on the extract solvent.

Effect of mushroom dried powder addition on the quality of soybean curd (버섯 건조분말 첨가에 따른 두부의 품질 특성)

  • Kim, Jeong-Han;Lee, Yun-Hae;Cho, Seong-Choon;Ju, Young-Cheoul
    • Journal of Mushroom
    • /
    • v.9 no.1
    • /
    • pp.39-43
    • /
    • 2011
  • Soybean curd was mixed with mushroom powder to develop new healthful foods, and changes in quality characteristics were investigated. The yields of the soybean curds containing 3~12% powder of Pleurotus ostreatus(PPo), 3, 6% of powder of Pleurotus cornucopiae(PPc), Pleurotus salmoneostramineus(PPs), Agrocybe aegerita(PAa) were higher than those of the control. The hardness level was highest in the 3% of dried mushroom power, The addition of mushroom powder to curd evidenced a significantly lower L value(lightness) than was detected in the control. In particular, PPs added soybean curd observed the highest a value(redness). In terms of overall acceptability, the preferred soybean curd samples were the 6% PPo, 3% of PPc, PPs and, PAa.

Historical Record of Mushroom Research and Industry in Korea

  • Yoo, Young Bok;Oh, Youn Lee;Shin, Pyung Gyun;Jang, Kab Yeul;Sung, Gi-Ho;Kong, Won-Sik
    • 한국균학회소식:학술대회논문집
    • /
    • 2014.05a
    • /
    • pp.13-13
    • /
    • 2014
  • Two kinds of mushrooms, Gumji (金芝; Ganoderma) and Soji, were described in old book of Samguksagi (History of the three kingdoms; B.C 57~A.D 668; written by Bu Sik Kim in 1145) in Korea-dynasty. Many kinds of mushrooms were also described in more than 17 kinds of old books during Chosun-dynasty (1392~1910) in Korea. Nowadays, mushroom cultivation has been increased through out the world last decade years. Production of mushrooms has also been increased 10-20% and many varieties have been cultivated. Similar trends were also observed in Korea. Approximately two hundred commercial strains of 37 species in mushrooms were developed and distributed to cultivators. Somatic hybrid variety of oyster mushroom 'Wonhyeong-neutari' were developed by protoplast fusion, and distributed to grower in 1989. The fruiting body yield index of somatic hybrids of Pleurotus ranged between 27 and 155 compared to parental values of 100 and 138. In addition, more diverse mushroom varieties such as Phellinus baumi, Auricularia spp., Pleurotus ferulae, Hericium erinaceus, Hypsizigus marmoreus, Grifola frondosa, Agrocybe aegerita and Pleurotus cornucopiae have been attempted to cultivate in small scale cultivation. Production of mushrooms as food was 190,111 metric tons valued at 800 billion Korean Won (one trillion won if include mushroom factory products; 1dollar = 1,040 Won) in 2011. Major cultivated species are Pleurotus ostreatus, Pleurotus eryngii, Flammulina velutipes, Lentinula edodes, Agaricus bisporus, and Ganoderma lucidum, which cover 90% of total production. Since mushroom export was initiated from 1960 to 1980, the export and import of mushrooms have been increased in Korea. Technology developed for liquid spawn production and automatic cultivation systems lead to the reduction of the production cost resulting in the increasement of mushroom export. However some species were imported because of high production cost for these mushrooms requiring the effective cultivation methods. Developing of effective post-harvest system will be also directly related to mushroom export. In academic area, RDA scientists have been conducting mushroom genome projects. One of the main results is the whole genome sequencing of Flammulina velutipes for molecular breeding. An electrophoretic karyotype of of F. velutipes was obtained using CHEF with 7 chromosomes, with a total genome size of approximately 26.7 Mb. The mususcript of the genome of F. velutipes was published in PLOS ONE this year. For medicinal mushrooms, we have been conducting the genome research on Cordyceps and its related species for developing functional foods using this mushroom. In 2013, Korea Food and Drug Administraion (KFDA) approved Cordyceps mushroom for its value as an immune booster.

  • PDF

Fruiting body development and genetic analysis of somatic hybrids by protoplast fusion in edible fungi (식용버섯의 원형질체 융합체의 자실체 발생 및 유전분석)

  • Yoo, Young Bok;Kong, Won Sik;Oh, Se Jong;Jhune, Chang Sung;Shin, Pyung Gyun;Kim, Beom Gi;Kim, Gyu Hyun;Park, Minsun;Min, Byung Re
    • Journal of Mushroom
    • /
    • v.2 no.3
    • /
    • pp.115-126
    • /
    • 2004
  • Somatic hybrids of inter-compatible and inter-incompatible strains were obtained by protoplast fusion. The fusion products between compatible strains, Pleurotus ostreatus and P. florida, formed heterokaryons, while fusants between incompatible strains such as P. cornucopiae + P. florida, P. ostreatus + Ganoderma applanatum, P. florida + Ganoderma lucidum, and P. ostreatus + Flammulina velutipes formed synkaryons that retained genes from both parents. The heterokaryons showed the same level of basidioma development. In contrast, the synkaryons showed unique characteristics including clamp connection formation at mitosis, either partner basidioma development, and abnormal segregation and recombination compared with inter-compatible strains. Synkaryons can be classified into homokaryoyic and heterokaryotic type. A comparison of somatic hybrids with compatible and incompatible strains was made using random amplified polymorphic DNA (RAPD) analysis. The heterokaryons between compatible species showed the same level of variability and contained both parental RAPD bands. In contrast, most of the synkaryons between incompatible species showed similarity to those of either parental bands and non-parental RAPD bands. Synkaryons can be classified into microgenome insertion type and macrogenome insertion type. A tetrapolar mating system was found among monospore isolates in somatic hybrids and wild type P. ostreatus. Homokaryons from each somatic hybrid combination were paired with tester homokaryons of the initial wild type of P. ostreatus. The changed mating types were identified in progenies. The pattern of mating type switching in somatic hybrids depends on compatibility of fusion partner. There are several factors related to the mechanism of clamp connection formation and fruiting body development of synkaryons. Of these,the major factor may be associated with self-fertility and mating type switching such as homokaryotic fruiting of wild type P. ostreatus. This review will discuss these aspects.

  • PDF

Analysis of amino acids in Golden mushroom:"Gumbit" (Pleurotus cornucopiae var. citrinopileatus) and Pink mushroom:"Noeul"(Pleurotus salmoneostramineus) (노랑느타리 "금빛" 과 분홍느타리 "노을"의 아미노산 분석)

  • Noh, Hyung-Jun;Suh, Jang-Sun;Kwon, Jang-Sik;Weon, Hang-Yeon;Lee, Si-Young;Yoo, Young-Bok;Jhune, Chang-Sung;Jang, Kab-Yeul;Seok, Soon-Ja
    • Journal of Mushroom
    • /
    • v.6 no.3_4
    • /
    • pp.111-114
    • /
    • 2008
  • This study was carried out to investigate amino acid contents of golden mushroom and pink mushroom. The amino acid analysis was followed by AccQ-Tag method and HPLC on gradient conditions. Seventeen amino acids were anal)'zed and sixteen amino acids were found in golden mushroom; fifteen amino acids in pink mushroom respectively. Among total amino acid in golden mushroom, cysteine content was the highest and glycine, glutamic acid, proline were followed. Among total amino acid in pink mushroom, cysteine was the highest and glycine, lysine, methionine were followed. In our result, concerning amino acids, cysteine was dominant. and alanine was detected in golden mushroom but pink mushroom.

  • PDF