• 한국균학회지
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• 제19권4호
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• pp.299-305
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• 1991

Simultaneous distillation-extration method에 의해 4종의 국내에서 인공재배되고 있는 느타리 버섯류 (Pleurotus sp)로부터 휘발성 향기성분을 분리한 다음 GC-MS 및 GC에 의한 표준품과의 머무름 시간 비교에 의해 성분을 확인 하였다. 확인된 54종의 휘발성 화합물중 저온성 느타리 (Pleurotus ostreatus ASI 201)에서는 1-octen-3-ol, 3-octanone, 3-octanol등이, 원형 느타리 (Pleurotus florida--ostreatus-ostreatus)에서는 1-octen-3-ol, n-hexanol, 3-octanone, 3-octanol, phenol등이, 애느타리 (Pleurotus ostreatus)에서는 1-octen-3-ol, n-pentanal, n-hexanol, n-pentanol, 3-octanone, 3-octanol, 1,5-octadien-3-one등이었다. 특히 확인된 성분중에는 탄소수가 8개인 alcohol 및 carbonyl화합물이 많았으며 전체 향기성분에 대한 $C_8$화합물의 비율은 각각 56.60%, 72.46%, 54.84%, 35.85%이었다.

• 한국균학회지
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• 제25권1호통권80호
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• pp.20-25
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• 1997

느타리버섯 균주(Pleurotus isolates)와 이들 균주들을 교잡하여 얻어진 균사에서 추출한 단백질을 전기영동하여 균주간의 유전적 유연관계를 밝힌 결과, P. ostreatus 201은 자가교잡 균주 혹은 타가교잡 균주들과 낮은 유연관계를 나타냈다. P. ostreatus 2042도 역시 동일한 결과를 나타냈다. 그러나, P. ostreatus $2042{\times}P$. ostreatus 202, P. ostreatus $2042{\times}P$. sajor-caju, 그리고 ostreatus $2042{\times}P$ ostreatus 900은 높은 상관관계를 나타냈다. P. ostreatus 202와 P. ostreatus 900은 자가교잡 균주 혹은 타가 교잡된 균주들과 낮은 상관관계를 나타내었다. 그러나 자가 교잡된 P. ostreatus와 P. $ostreatus{\times}P$. florida, 그리고 P. florida와 자가교잡된 P. florida는 각각 서로 높은 상관관계를 나타냈다.

• 동아시아식생활학회지
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• 제11권3호
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• pp.196-203
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• 2001

This study was conducted to provide basic data for the development of natural seasoning. The quality chatacteristics of water exacts from Pleurotus ostreatus and Lentinus edodes were determind. Also, savory taste, fragrance, color and overall preference was evaluated. The preference test on the seasonings prepared from Pleurotus ostreatus and Lentinus edodes with the addition of sea tangle was also performed. The crude protein content of dried, Lentinus edodes and Pleurotur ostreatus was 20.4% and 30.4% respectively. The total organic acid contents were higher in Plourotus ostreatus than in Lentinus edodes extracts. The total free amino acid adn total nucleotide contents were higher in Pleurotus ostreatus than in Lentinus edodes extracts. Three nucleotide, adenosine monophosphate(AMP) , guanosine monophosphate(GMP), xanthine monophosphate(XMP) were found in mushroom seasonings while inosine monophosphate(IMP) was not detected. Pleurotus ostreatus extract showed higher score in savory taste and overall acceptability than Lentinus edodes extract, Pleurotur ostreatus seasoning added with 1% sea tangle and Lentinus edodes seasoning added with 1.5% sea tangle were rated as high in overall quality In a sensory test of foods cooked with mushroom seasoning. It was shown that panels in their twenties well used to the taste of monosodium glutamate while the people in their thirties preferred the specific flavor of the mushroom seasoning.

• 한국유기농업학회지
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• 제25권4호
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• pp.749-758
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• 2017

This Research was the standardization of nutrient sources by reviewing the possibility of cultivation and analysis of imported nutrient sources used in Pleurotus ostreatus culture. As a first result, the concentration of heavy metals was not detected or trace, and the total nitrogen content of cotton seed pomace was 6.22% in Australia, which was higher than that of Chinese or USA. As a second result of physical properties of nutrient sources, the water holding capacity of beet pulp from Egypt was higher with increasing pressure. The volume water content of US was highest at 400hpa and 500hpa in case of cottonseed meal. Third, the content of total nitrogen was the highest at pH 5.51 and the total nitrogen content was the highest at 2.45, and the C/N ratio was the highest at 28.18. Last result shows that first germination day, yield and biological effciency of all treatments were not significant differnet except T2. These results shows that australian cootton was inappropriate to Pleurotus ostreatus culture. Nevertheless, the safety and standardization of the Pleurotus ostreatus culture medium should be assured by reviewing the media low material importing season, mushroom yield and nutrient sources.

• 한국식품위생안전성학회지
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• 제7권4호
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• pp.157-160
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• 1992

쑥으로부터 추출한 정유를 Escherichia coli, Bacillus subtilis , Pleurotus ostreatus , Fusarium solani , Aspergillus nidulans에 첨가한 후 배양한 결과 다음과 같은 결과를 얻었다. 쑥의 정유를 첨가한 후 미생물을 배양시킨 결과 Escherichia coli,는 무반응이었으나 Bacillus subtilis , Pleurotus ostreatus , Fusarium solani , Aspergillus nidulans는 생육이 저해되었다. bacillus subtilis는 대조군에 비해 정유의 농도가 10~100ppm에서 10배의 생육저해를 나타냈다. 쑥의 정유는 또한 곰팡이에서 강한 생육저해를 나타냈으며 Pleurotus ostreatus는 1,000ppm의 농도에서 생육이 완전히 정지되었다.

• Applied Biological Chemistry
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• 제47권1호
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• pp.22-26
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• 2004

리그닌 분해균인 느타리 버섯균(Pleurotus ostreatus) K-2946을 glucose-peptone-yeast(G-P-Y) 액체배지에서 배양 하여 망간퍼옥시데이즈를 생산하였다. 본 실험조건 하에서 리그닌 퍼옥시데이즈는 생산되지 않았다. 느타리버섯균 K-2946이 생산하는 망간 퍼옥시데이즈를 이온크로마토그래피, 겔크로마토그래피를 이용하여 정제하였다. 정제한 망간 퍼옥시데이즈의 분자량은 36400 Da이였고 pI는 3.95였다. 정제된 효소의 최적 pH는 5.0이었으며 최적온도는 $55^{\circ}C$이었다.

• 한국식품과학회지
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• 제20권4호
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• pp.459-462
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• 1988

국내에서 널리 인공재배되고 있는 식용버섯인 느타리버섯(Pleurotus ostreatus), 표고버섯(Lentinus edodes) 및 양송이(Agaricus bisporus)의 유리당 및 당알코올을 GCL 법으로 분석하여 버섯의 종류. 부유 및 조기별 차이점을 비교 정토한 결과는 다음과 같다. 3종의 버섯에서 trehalose, glucose, fructose, mannitol, arabitol 및 glycerol등이 확인되었다. 유리당 및 당알코올의 충 함량은 표고버섯 $12.7{\sim}18.9%$, 양송이 $9.2{\sim}16.1%$, 느타리버섯 $8.2{\sim}10.6%$ 순 이었고 표고버섯에서는 mannitol과 arabitol, 양송이에서는 mannitol, 느타리버섯에서는 tehalose와 mannitol이 특징적으로 많이 함유되어 있었다. 부위별로 비교했을때 느타리버섯과 양송이는 자루보다 갓부위에 많았으나 표고버섯은 자루부위에 많았고 크기별로는 크기가 클수록 대체로 유리당 및 당알코올 함량이 감소하는 경향이었다.

• 한국약용작물학회지
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• 제18권2호
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• pp.113-117
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• 2010

The antioxidant and anticancer activities of Phellinus linteus, Inonotus obliquus, and Pleurotus ostreatus according to extraction methods were measured. The contents of polyphenolic compounds were higher in fermented extracts than those in water extracts. The contents were 192.4mg/100 $m{\ell}$ for Inonotus obliquus vinegar, 186.9 mg/100 $m{\ell}$ for Phellinus linteus vinegar, and 156.5 mg/100 $m{\ell}$ for Pleurotus ostreatus vinegar, respectively. EDA(electron donating ability) in Phellinus linteus that was highest among mushrooms was 83.9% in the water extract and 96.8% in the fermented extract. The growth of stomach cancer cells, SNU-719 was inhibited 62.4%, 65.5%, and 53.0% by Phellinus linteus vinegar, Inonotus obliquus vinegar, and Pleurotus ostreatus vinegar, respectively and also the growth of liver cancer cells, Hep3B was inhibited 67.1%, 68.3%, and 57.6% by Phellinus linteus vinegar, Inonotus obliquus vinegar, and Pleurotus ostreatus vinegar, while the growth of normal cell, DC2.4 was not affected. Even though Phellinus linteus and Inonotus obliquus showed higher inhibition in cancer cell growth, Pleurotus ostreatus can be efficiently used for antioxidant and anticancer activities due to their cheap price in the market.

• 한국균학회지
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• 제27권5호
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• pp.328-336
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• 1999

Isozyme comparisons of mycelial extracts from Pleurotus ostreatus were undertaken using isoelectric focusing. Enzyme isozyme patterns were Used to describe the extent of geographical diversity and degree of intraspecific variation in these extracts. A total of 77 bands were resolved from six different enzymes. Cluster analyses were performed using the zymograms for esterase (EST), glucose phosphate isomerase (GPI), leucine aminopeptidase (LAP), malate dehydrogenase(MDH), peroxidase (POX), and phosphoglucomutase (pGM). EST gave multiple banding patterns, while less variability was observed for GPI, MDH, and PGM. Cluster analyses demonstrated that strains of P. ostreatus from geographically different origins are genetically divergent, supporting the idea that there is little or no gene flow between these geographically distant population groups.

• 한국미생물·생명공학회지
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• 제13권1호
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• pp.65-70
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• 1985

The production media, partial purification and the enzymatic characteristics of laccase from Pleurotus ostreatus were investigated. Among various media tried the double strength onion media showed much higher production of laccase compared to glucose and/or CMC added media. The laccase from Pleurotus ostreatus has the optimum pH of 5.94 for the activity and appears to be stable at relatively broad pH spectrum (4.7-8.7). The experiments on the temperature stability shows that above 90% activity could be preserved after holding at $30^{\circ}C$ for 40 min., 60% activity was remained after 40 min. at $50^{\circ}C$. The Km value of the laccase from Pleurotus ostreatus was estimated to be 3.209mM and the molecular weight of laccase was estimated to be 55,000 by SDS-Polyacrylamide gel electrophoresis.