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Production and Characterization of Manganese Peroxidase from the White Rot Fungus Pleurotus ostreatus in Liquid Culture  

Lee, Jae-Sung (Department of Food Science and Technology, Yeungnam University)
Ha, Hyo-Cheol (Institute of Food & Culture, Pulmuone Co. Ltd.)
Publication Information
Applied Biological Chemistry / v.47, no.1, 2004 , pp. 22-26 More about this Journal
Abstract
The ligninolytic basidiomycete, Pleurotus ostreatus K-2946, was produced a manganese peroxidase (MnP) activity when grown in liquid culture with glucose-yeast-peptone (G-Y-P) medium. However, lignin peroxidase (LiP) was not detected in this culture medium. The purification progress of MnP was purified that included chromatography on Sepharose CL-6B, Superdex 75 prep grade and Mono-Q. MnP purified by column chromatography, was 36400 dalton and a pI of 3.95. The optimal pH and temperature of the purified MnP activity were 5.0 and $55^{\circ}C$. The characteristics of MnP produced was quite similar to those of MnP 3 isoenzyme produced by other strains of P. ostreatus.
Keywords
Pleurotus ostreatus; manganese peroxidase; liquid culture;
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