• Korean Journal of Pharmacognosy
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• v.36 no.1 s.140
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• pp.56-59
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• 2005

Urease plays an important role in the urea metabolism and the effect of urease activity on human and environment is enormous. For instance, urease acts as a virulence factor of the urinary and gastrointestinal tracts infections in human and animal, being involved in kidney stone formation, catheter encrusatation, pyelonephritis, ammonia encephalopathy, hepatic coma, and urinary tract infections. Widespread urease activity in soil induces a plant damage due to ammonia toxicity and pH increase. Therefore, urease activity regulation through urease inhibitors would lead to an enhanced efficiency of urea nitrogen uptake in plants and to the improved therapeutic strategies for ureolytic bacterial infections. To search for new inhibitory compounds on urease activity from herbs, MeOH extracts of herbs were screened. Among of them, the MeOH extracts of Areca catechu exhibited an excellent inhibitory effect on urease activity. Two compounds were isolated from the ethyl acetate fraction by the activity guided fractionation. Their chemical structures were identified as (+)-catechin(compound I) and allantoin(compound II) by spectroscopic evidence, respectively. Compound I showed a stronger inhibitory effect on urease activity than compound II.

• Korean Journal of Soil Science and Fertilizer
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• v.41 no.3
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• pp.184-189
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• 2008

To assess the relationship between Cd fraction in paddy soils and Cd in polished rice, soils and rice were analyzed at the 3 Cd contaminated paddy fields near closed mines. Major Cd fractions of A field were organically bound (62.6%) and Fe-Mn oxide bound (25.3%) forms. In case of B field, major Cd fractions of B1 field were carbonate bound (46.3%) and Fe-Mn oxide bound (31.6%) form whereas B2 field were residual (54.3%) and carbonate bound (21.8%) form, respectively. It showed a huge difference of Cd fraction each other. 0.1M HCl extractable Cd in soil was positively correlated with Cd in rice. Specially, the ratios of 0.1M HCl extractable Cd against total Cd content in soils were 13.7%, 2.6%, and 0.45% in A, B1, and B2 fields, respectively. These ratio were largely affected with Cd uptake to rice grain. Also, exchangable, Fe-Mn oxide bound, and carbonate bound form, which are partially bioavailable Cd fraction to the plant, were positively correlated with Cd in rice while organically bound and residual form was not correlated. Multiple regression equation was developed with Rice Cd = -0.02861 + 0.07456 FR 1(exchangeable) + 0.00252 FR 2(carbonate bound) + 0.001075 FR 3(Fe Mn oxide bound) - 0.00095 FR 4(organically bound) - 0.00348 FR 5(residual) ($R^2=0.7893^{***}$) considering Cd fraction in soils.

• CELLMED
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• v.4 no.4
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• pp.27.1-27.5
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• 2014

Picrorhiza kurroa Royle ex Benth. (Scrophulariaceae) is a traditional Ayurvedic herb known as Kutki. It is used as a remedy for diabetes by tribes of North Eastern Himalayan region of India. Present study was conducted to explore the mechanism of antidiabetic activity of standardized aqueous extract of Picrorhiza kurroa (PkE). PkE (100 and 200 mg/kg/day) was orally administered to streptozotocin induced diabetic rats, for 14 consecutive days. Plasma insulin levels were measured and pancreas of rat was subjected to histopathological investigations. Glucose transporter type 4 (GLUT-4) protein content in the total membrane fractions of soleus muscle was estimated by Western blot analysis. Plasma insulin level was significantly increased along with concomitant increase in GLUT-4 content of total membrane fractions of soleus muscle of diabetic rats treated with extract. There was evidence of regeneration of ${\beta}$-cells of pancreatic islets of PkE treated group in histopathological examinations. PkE increased the insulin-mediated translocation of GLUT-4 from cytosol to plasma membrane or increased GLUT-4 expression, which in turn facilitated glucose uptake by skeletal muscles in diabetic rats.

• Korean Journal of Agricultural Science
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• v.40 no.3
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• pp.177-182
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• 2013

A genus Camelina has been attracted as a promising oil crop, especially available in drought and marginal conditions. Due to more demands on arable land for bioenergy crops, price of agricultural products has been a challengeable issue. In that respect, development of Camelina crop with higher germination rate and germination energy can be a strategy to secure seedling establishment, nutrient uptake and long vegetative period. In order to be easily available in the field and laboratory conditions, Camelina seed needs to be optimized for its germination temperature. Germination temperature regime was in a range of 8 to $32^{\circ}C$ initially, and consecutively narrowed down to 8 to $20^{\circ}C$. Based on the temperature range, Camelina germinated greater than 96% at $8-16^{\circ}C$ in two weeks after sowing, but germination rate started to decrease at the higher than $24^{\circ}C$ and was significantly low at higher than $32^{\circ}C$. In terms of rapid time to reach the maximum germination rate and greater germination energy, temperature ranged from 12 to $16^{\circ}C$ was found to be desirable for Camelina germination. Although germinationa rate was greater at $16^{\circ}C$, lower temperature close to $12^{\circ}C$ would be favored for the field conditions where greater root growth leading to healthier seedlings and better nutrient or water availability is considerably demanded.

• Korean Journal of Medicinal Crop Science
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• v.24 no.3
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• pp.198-206
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• 2016

Background: Electrical conductivity (EC) and pH are important features of nutrient solution, affecting both growth and quality of crops by altering nutrient uptake. Methods and Results: The pH values of nutrient solutions were controlled at 5.0, 5.5, 6.0, 6.5 and EC values were controlled at 0.68, 0.84, 1.23, 1.41 dS/m. Gingesng root weights were higher during the initial growth period when the plants were treated with low pH and low EC nutrient solutions. However, the higher pH and EC levels, the greater the increase in the rate of root weight between the initial and middle growth periods. The highest ginsenoside amount changed during growth period. The total ginsenoside amount was highest in the root, and the lowest in leaves at 45 and 90 days after treatment, respectively, with solution at a pH of 6.0. After 135 days of treatment, the highest total ginsenoside amount was detected in root treated with soluton with EC values of 1.23 dS/m. Conclusions: For the cultivation of ginseng using a nutriculture system, the pH and EC values of nutrient solutions should to be controlled based on the stage of growth and targeted plant organ (root or leaves).

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.3
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• pp.242-250
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• 2016

Veterinary antibiotics (VAs) has been used to treat animal disease and to increase animal weight as growth promoter. However, abused usage of VAs can cause production of antibiotic resistance genes (ARGs) in the environment and additionally, residual of VAs in soil can be transferred into crops. Therefore, main objective of this research was to examine bioaccumulation of VAs in sprouts (red cabbage, Brassica Olearacea L. var. Capitata f. rubra and red radish, Raphanus sativus) with hydroponic method. Total of 7 VAs in 2 different classes of VAs (tetracyclcines: tetracycline, oxytetracycline, chlortetracycline, sulfonamides: sulfamethoxazole, sulfamethazine, sulfamethiazole, macrolides: tylosin) were evaluated and experiment was conducted with solid phase extraction (SPE)/high performance liquid chromatography tandem mass spectrometry (HPLC/MS/MS). Initial spiked concentration of 7 VAs was $5mg\;L^{-1}$ and cultivation period was 8 days. Result showed that growth of sprouts was inhibited about 23-27% when VAs was introduced. Amount of bioaccumulated VAs was also differed depending on class of VAs. The highest amount of bioaccmulated VAs was tetracycline and sulfamethoxazole in each class with a concentration of 4.05, $7.73mg\;kg^{-1}$ respectively. Calculated transfer ratio of VAs into crops was also ranged 0.38-54.27%. Overall, bioaccumulation of VAs in crops can be varied depending on crop species and class of VAs. However, further research should be conducted to verify bioaccumulation of VAs in crops in the soil environment.

• Journal of Life Science
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• v.20 no.7
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• pp.1041-1046
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• 2010

Phytoextraction is a technique which uses plants to clean up metal-contaminated soils. Recently, various chelating agents were introduced into this technique to increase the bioavailability of metals in soils. Even though the technique is an economic and environment-friendly method, this cannot be applied in highly metal-contaminated areas because plants will not normally grow in such conditions. Therefore, this research focuses on identifying chelating agents which are biodegradable and applicable to highly metal-contaminated areas. Alunimum (Al) as a target metal and cysteine (Cys), histidine (His), citrate, malate, oxalate, succinate, and ethylenediamine (EDA) as biodegradable chelating agents were selected. Ethylenediamine tetraacetic acid (EDTA) was used as a comparative standard. Plants were grown on agar media containing various chelating agents with Al to analyze the effect on plant growth. His slightly diminished the inhibitory effect of Al on root growth of plants, whereas, Cys, citrate, malate, oxalate, and succinate did not show significant effects. Both EDTA and EDA strongly diminished the inhibitory effect of Al on root growth. The effect of EDA is correlated with decreased Al uptake into the plants. In conclusion, as a biodegradable chelating agent, EDA is a good candidate for highly Al-contaminated areas.

• Journal of the Korean Recycled Construction Resources Institute
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• v.3 no.2
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• pp.115-121
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• 2015

The objective of this study is to present the design approach of low $CO_2$ concrete structures for reduction of $CO_2$ emissions. The design approach was implemented considering the system boundary for each processing presented in the ISO 13315-2. As for life-cycle inventory(LCI) for $CO_2$ assessment of concrete structures, data provided from domestic LCI DB was used. Based on the process presented in this study, case studies on the life-cycle $CO_2$ assessment of shear wall concrete structure was conducted. As substitution level of GGBS is 25%, the amount of $CO_2$ emissions and $CO_2$ uptake by concrete carbonation was decreased in the material, demolition and crushing, and transport phase. The amount of $CO_2$ emissions of column and total member was decreased by 26% and 22% respectively, compared to that of OPC.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.1
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• pp.32-40
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• 2006

The nitrogen (N) absorption and partition of the rice plants are important indicators that can be used to improve the N use efficiency (NUE) of the plants. Improving the plant NUE can help to avoid nutrient waste that may cause environmental pollution. To investigate the N absorption and partition of the rice plants, Hwaseongbyeo (Japonica) and Dasanbyeo (indica/japonica) were applied with N fertilizers at the rates of 60, 120, and 180 kg N per ha in paddy field. Also micro plots of $0.81m^2$ were established inside each plot for application of $^{15}N-labeled$ fertilizer. The differences in N utilization of the rice plants were associated with the total N absorption and partitioning after the heading stage. In the grain filling period, the increase of nitrogen content in the total and leaf blades of Dasanbyeo was higher than that of Hwaseongbyeo. Soil N was the main contributor for the increase of total N of Dasanbyeo during the grain filling period. The N fertilizer uptake rate of Hwaseongbyeo rapidly increased with the increment of N fertilization rate. In Dasanbyeo, N fertilizer uptakes were similar under all rates and times of N application. From heading stage to harvest, Dasanbyeo continued accumulating nitrogen, whereas Hwaseongbyeo had small changes. In conclusion, the difference in nitrogen absorption and partition after heading of the two cultivars was caused by the ability of Dasanbyeo to accumulate and remobilize soil nitrogen to the grains during the grain filling period.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 2001.04b
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• pp.73-74
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• 2001

순환식 펄라이트 재배에서 오이의 양액흡수는 일사량 변화와 관계없이 단위 일사량당 흡수량이 80-100mgㆍMJ$^{-1}$까지 증가 후 일정하게 유지되어 양액흡수 지표는 전체 양액흡수량보다 단위일사량당 양액흡수량이 더 적합하였다(Fig. 1). 오이의 엽장$\times$엽폭 수치와 실측 엽면적과의 관계는 Y=-16.87＋0.987x＋5E-5x$^2$의 회귀식을 구할 수 있었으며 $r^2$=0.89의 높은 정의 상관을 보였다. NO$_3$$^{-}$ -N의 흡수량은 초기에 3mgㆍMJ$^{-1}$에서 후기 16mgㆍMJ$^{-1}$로 상승하였고 Ca는 초기에 3mgㆍMJ$^{-1}$에서 후기에 14mgㆍMJ$^{-1}$로, Mg는 초기에 1mgㆍMJ$^{-1}$에서 후기에 5mgㆍMJ$^{-1}$로 증가되었으나, 정식 후 62일 이후의 증가세가 둔화되었다. K는 초기에 5.0mgㆍMJ$^{-1}$에서 후기 18mgㆍMJ$^{-1}$로 증가되었으나 지속적인 증가를 보여주지 못하였는데 이것은 오이의 하엽 제거로 인한 결과로 생각되어진다. 그러나 P는 초기에 0.5mgㆍMJ$^{-1}$에서 후기에 3.2mgㆍMJ$^{-1}$로 지속적으로 증가되었다. S는 초기에 0.5mgㆍMJ$^{-1}$에서 중기에 6.5mgㆍMJ$^{-1}$까지 증가되다가 후기에 2.7mgㆍMJ$^{-1}$로 감소되었다(Fig. 2). 오이의 각각의 무기이온 흡수량과 가장 상관이 높았던 요소는 정식일수와 엽면적이었으며 이 두 요소와 단위일사량당 양액흡수량과는 $r^2$=0.92, 0.97로 높은 상관을 보였다(Table 1). 단위일사량당 양액흡수량을 이용한 각각의 무기이온 흡수량 회귀식은 $r^2$=0.8 이상으로 높은 상관관계를 보여 실용적 이용이 가능할 것으로 보였다(Fig. 3).