Browse > Article

Isolation of Urease Inhibitory Compounds from Arecae Semen  

Ryu, Jei-Man (Central Research Institute, Dong-Wha Pharm. Ind.)
Jang, Hwan-Bong (Central Research Institute, Dong-Wha Pharm. Ind.)
Rho, Yang-Kook (Central Research Institute, Dong-Wha Pharm. Ind.)
Oh, Seong-Jun (Central Research Institute, Dong-Wha Pharm. Ind.)
Lee, Hyun-Yong (Central Research Institute, Dong-Wha Pharm. Ind.)
Leem, Moon-Jeong (Central Research Institute, Dong-Wha Pharm. Ind.)
Publication Information
Korean Journal of Pharmacognosy / v.36, no.1, 2005 , pp. 56-59 More about this Journal
Abstract
Urease plays an important role in the urea metabolism and the effect of urease activity on human and environment is enormous. For instance, urease acts as a virulence factor of the urinary and gastrointestinal tracts infections in human and animal, being involved in kidney stone formation, catheter encrusatation, pyelonephritis, ammonia encephalopathy, hepatic coma, and urinary tract infections. Widespread urease activity in soil induces a plant damage due to ammonia toxicity and pH increase. Therefore, urease activity regulation through urease inhibitors would lead to an enhanced efficiency of urea nitrogen uptake in plants and to the improved therapeutic strategies for ureolytic bacterial infections. To search for new inhibitory compounds on urease activity from herbs, MeOH extracts of herbs were screened. Among of them, the MeOH extracts of Areca catechu exhibited an excellent inhibitory effect on urease activity. Two compounds were isolated from the ethyl acetate fraction by the activity guided fractionation. Their chemical structures were identified as (+)-catechin(compound I) and allantoin(compound II) by spectroscopic evidence, respectively. Compound I showed a stronger inhibitory effect on urease activity than compound II.
Keywords
Areca catechu; Arecae Semen; urease; urease inhibitory activity; (+)-catechin; allantoin;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Moblely, H. L. T. and Hausinger R. P. (1989) Microbial urease: significance, regulation, and characterization. Micrbiol. Rev. 53: 85-108
2 Inokuchi, I., Okabe, H., Yamauchi, T., Naganstsu, A., Nonaka, G. and Nishioka, I. (1986) Antihypertensive substance in seeds of Areca catechu. Life Sci. 38: 1375-1382   DOI   ScienceOn
3 Lalithakumari, H. and Sirsi, M. (1965) Antibacterial and antifungal activities of Areca catechu Linn. Indian J. Exp Biol. 31: 66-67
4 Inokuchi, I., Okabe, H., Yamauchi, T. Nagamatsu, A., Nonaka, and G. Nishioda, I. (1985) Inhibitors of Angiotensinconverting enzyme in crude drugs II. Chem. Pharm. Bull. 33: 264-269   DOI   ScienceOn
5 Reimann, H. J., Lorenz, W., Fischer, M., Frolich, R., Meyer, H. J. and Schmal, A. (1977) Histamine and acute haemorrhagic lesions in rat gastric mucosa: prevention of stress ulcer formation by (+)-catechin, an inhibitor of specific histidine decarboxylase in vitro. Agents Actions. 7: 69-73   DOI   ScienceOn
6 Gryglewski, R. J., Korbut, R., Robak, J. and Swies, J. (1987) On the mechanism of antithrombotic action of flavonoids. Biochem. Pharmacol. 36: 317-322   DOI   ScienceOn
7 Bensky, L. (1992) Chinese Herbal Medicine, 197. Eastland Press, Seattle
8 박종희(2002) 한약백과 도감, 363-365. 신일상사, 서울
9 Hans Ulrich Bergmeyer (1974) Methods of Enzymatic Analysis Vol 4, 1791-1798, Verlag Chemie Weinheim Academic Press, New York and London
10 Collins, C. M. and D'Orazio, S. E. (1993) Bacterial ureases: structure, regulation of expression and role in pathogenesis. Mol. Microbiol. 9: 907-913   DOI   ScienceOn
11 Hausinger, R. P. (1993) Biochemistry of Nickel, 23-57. Plenum. New York, NY
12 Bremner,J. M., Mulvaney, R. L., and Burns(Ed) R. G., (1978), Soil Enzymes, 149-196. Academic Press. New York, NY
13 Fisher, A. A. (1981) Allantoin: a non-sensitizing topical medicament. Therapeutic effects of the addition of 5 percent allantoin to vaseline. Cutis. 27: 230-231, 234, 329
14 Klopman, G and DiMayuga, M. L. (1988) Computer-automated structure evaluation of flavonoids and other structurally related compounds as glyoxalase I enzyme inhibitors. Mol. Pharmacol. 34: 218-222