• Title/Summary/Keyword: Plant diseases

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The development of transgenic maize expressing Actinobacillus pleuropneumoniae ApxIIA gene using Agrobacterium (아그로박테리움을 이용한 Actinobacillus pleuropneumoniae ApxIIA (ApxII toxin) 유전자 발현 옥수수 형질전환체 개발)

  • Kim, Hyun-A;Yoo, Han-Sang;Yang, Moon-Sik;Kwon, Suk-Yoon;Kim, Jin-Seog;Choi, Pil-Son
    • Journal of Plant Biotechnology
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    • v.37 no.3
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    • pp.313-318
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    • 2010
  • To develop edible vaccines for swine, the embryogenic calli (type II) derived from HiII genotype were inoculated with A. tumefaciens strain C58C1 containing the binary vector pMYV611, 613, 616, and V621, 622 and 623 respectively. Six of those vectors carry nptII gene which confers resistance to paromomycin and apxIIA gene producing ApxII toxin which is generated in various serum types of A. pleuropneumoniae as a target gene. The 4,120 callus clones for pMYV611, 5,959 callus clones for pMYV613, 7,581 callus clones for pMYV616, 52,329 callus clones for V621, 48,948 callus clones for V622, and 56,188 callus clones for V623 were inoculated. The frequency of positive response clone was confirmed into range of 2.3% - 4.4% for each vectors by NPTII ELISA kit assay, and the selected callus clones of them were finally 3 callus clones from pMYV611 (0.07%), 4 callus clones from pMYV613 (0.07%), 2 callus clones from pMYV616 (0.03%), 51 callus clones from V621 (0.1%), 72 callus clones from V622 (0.15%), and 102 callus clones from V623 (0.18%) respectively. From the selected callus clones of each binary vector, the integration of the apxIIA gene into maize genome was detected from 2 plants of pMYV613 and 2 plants of V623 by Southern blot analysis.

Studies on the soybean varieties in Korea. -IV relationships between the seed yields and some characteristics on the several sowing dates- (대두의 품종에 관한 연구 -제4보 파종기별 수량과 제특성과의 관계-)

  • Kwon-Yawl Chang
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.2
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    • pp.30-37
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    • 1964
  • Experiments were carried out to clarify the relationships between seed yields and some ecological characteristics, seed yields and some morphological characteristics, and the relationships among the varietal yields on the several sowing plots when grown under different climatic conditions. Twenty-four soybean varieties were used as the material, selected at random from 138 varieties which were cultivated the year before. These varieties were grown in the Experimental Farm, Chinju Agricultural College, Kyung Sang Namdo, Korea, and seed sowing was conducted at 5 times, from April to July, in 1962 and 1963. The results obtained are summarized as follows: 1. Correlations between seed yields and some ecological characteristics on the sowing date bases are shown in Table 1. Among some ecological characteristics, it was observed that there wert close relationships between seed yields and days to flowering, and between seed yields and the total growing length, except on the late sowing, i.e. July sowing plots, However, there was no clear correlation between seed yields and durations of flowering, and between seed yields and days from flowering to maturity. 2. Correlations between seed yields and some morphological characteristics on the sowing date bases are shown in Table 2. From this table, it was also observed that there were some close relationships between seed yields and branch number for plant, stem diameter, plant weight, pod number per plant, and grain number per plant, but there was no clear relationship between seed yields and stem length, and between seed yields and 100 grain weight, on every sowing plot. 3. Relations between varietal yields and the sowing dates are shown in Table 3. Our leading varieties such as Ulsan, Sangdoo, Changdan Backmok, Iksan, Haman, Barchongdeo, and Anion Daerip, which belong to the maturity groups of IV, V, and VI, have been significantly high in yield at each sowing date plot, but the extreme early varieties, that frequently are damaged by insects, moisture and diseases, and the extreme late varieties, that frequently are caught by frost suffering reduced yield, are showed a lower yield than the others. However, extreme early varieties, for instance, Black Hawk, an American variety, and Chung Buk Baek, a Korean early variety, showed high productivity on the late sowing, June and July sowing plots, and Do san No. 6, which is late Japanese variety, showed a high yield on the extreme early sowing, i.e. April sowing plots. 4. Relations between seed yields and the sowing dates on the varietal bases are shown in Table 4. It was observed that, the early varieties did not differ much in yields at the various sowing dates while the yields of late varieties decreased progressively with subsequent dates. However, the extreme late varieties, such as Tamanishiki, that frequently are caught by frost suffering reduced yield, may be difficult to harvest at this location. The results in these experiments showed that in most cases maximum yields were obtained when leading varieties, which are medium or late varieties, were shown in Mid-May, and progressively lower yields were produced from June and July sowings. On the other hand, the late sowings of the early varieties, such as Black Hawk and Chung Buk Baek, in these experiments showed favorable yields compared with the others. From these results, we can predict that our local medium or late varieties produce higher yields at this location than was produced by early varieties in most of the sowing dates, especially the May sowing, and early varieties such as Black Hawk and Chung Buk Baek should be used in the late sowing, June sowing time.

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Studies on the soybean varieties in Korea -III. Relationships between the ecotypes and various characteristics- (대두의 품종에 관한 연구 -제3보 생태형과 제 특성간 그리고 수량과 제 특성간의 관계-)

  • Kwon-Yawl Chang
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.2
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    • pp.27-29
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    • 1964
  • Experiments were carried out to clarify the relationships between seed yields and some ecological characteristics, seed yields and some morphological characteristics, and the relationships among the varietal yields on the several sowing plots when grown under different climatic conditions. Twenty-four soybean varieties were used as the material, selected at random from 138 varieties which were cultivated the year before. These varieties were grown in the Experimental Farm, Chinju Agricultural College, Kyung Sang Namdo, Korea, and seed sowing was conducted at 5 times, from April to July, in 1962 and 1963. The results obtained are summarized as follows: 1. Correlations between seed yields and some ecological characteristics on the sowing date bases are shown in Table 1. Among some ecological characteristics, it was observed that there wert close relationships between seed yields and days to flowering, and between seed yields and the total growing length, except on the late sowing, i.e. July sowing plots, However, there was no clear correlation between seed yields and durations of flowering, and between seed yields and days from flowering to maturity. 2. Correlations between seed yields and some morphological characteristics on the sowing date bases are shown in Table 2. From this table, it was also observed that there were some close relationships between seed yields and branch number for plant, stem diameter, plant weight, pod number per plant, and grain number per plant, but there was no clear relationship between seed yields and stem length, and between seed yields and 100 grain weight, on every sowing plot. 3. Relations between varietal yields and the sowing dates are shown in Table 3. Our leading varieties such as Ulsan, Sangdoo, Changdan Backmok, Iksan, Haman, Barchongdeo, and Anion Daerip, which belong to the maturity groups of IV, V, and VI, have been significantly high in yield at each sowing date plot, but the extreme early varieties, that frequently are damaged by insects, moisture and diseases, and the extreme late varieties, that frequently are caught by frost suffering reduced yield, are showed a lower yield than the others. However, extreme early varieties, for instance, Black Hawk, an American variety, and Chung Buk Baek, a Korean early variety, showed high productivity on the late sowing, June and July sowing plots, and Do san No. 6, which is late Japanese variety, showed a high yield on the extreme early sowing, i.e. April sowing plots. 4. Relations between seed yields and the sowing dates on the varietal bases are shown in Table 4. It was observed that, the early varieties did not differ much in yields at the various sowing dates while the yields of late varieties decreased progressively with subsequent dates. However, the extreme late varieties, such as Tamanishiki, that frequently are caught by frost suffering reduced yield, may be difficult to harvest at this location. The results in these experiments showed that in most cases maximum yields were obtained when leading varieties, which are medium or late varieties, were shown in Mid-May, and progressively lower yields were produced from June and July sowings. On the other hand, the late sowings of the early varieties, such as Black Hawk and Chung Buk Baek, in these experiments showed favorable yields compared with the others. From these results, we can predict that our local medium or late varieties produce higher yields at this location than was produced by early varieties in most of the sowing dates, especially the May sowing, and early varieties such as Black Hawk and Chung Buk Baek should be used in the late sowing, June sowing time.

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Studies on the Patterns of Plastic Film House, Their Growing; Conditions, and Diseases and Pests Occurrence on Horticultural Crops in Southern Part of Korea. Insects and Nematodes Associated with Horticultural Crops and Effect of Nursery Soil Conditions on the Infection of Root-knot Nematode (남부지방(南部地方) 시설원예(施設園藝)의 유형(類型).재배환경(栽培環境) 및 병해충발생(病害蟲發生)에 관(關)한 연구(硏究) -충발생양상(蟲發集樣相)과 상토(床土)의 선택(選擇)에 따른 뿌리혹선충(線蟲)의 발생(發生)-)

  • Choo, Ho-Yul;Kim, Hee-Kyu;Park, Jung-Choon;Lee, Sang-Myeong;Lee, Jeong-Im
    • Korean journal of applied entomology
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    • v.26 no.4 s.73
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    • pp.195-201
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    • 1987
  • Insects and nematodes associated with crops growing in plastic film houses were surveyed throughout the southern part of Korea at Jinju, Jiphyeon, Geumsan, Hapcheon, Changyeong, Namji, Milyang, Kimhae, Busan, Sooncheon and Gwangyang from December of 1984 to December of 1985. The phytonematodes representing six families, nine genera and nine species, and the insects representing four orders, seventeen families, twenty-three genera and twenty-four species were identified. The mite also caused problem on the leaves of strawberry and watermelon. Of these Meloidogyne incognita and Aphis gossyphii were most important ones. Aphelenchoides fragariae and M. hapla, however dominant nematodes on strawberry. M. incognita was always detected from pepper plants which were heavily infected with Phytophthora capsici. Tomato roots were readily infected with root-knot nematodes in non-sterilized upland surface soil. However, tomato were growing-vigorouly free from nematode damage in the upland surface soil treated by nematicide or in the paddy soil. A few galls were developed even in the upland subsoil at 60cm below surface. Soil salinity affected profoundly the host-root-knot nematode interaction: the numerous galls were developed on the tomato roots at EC $1mS/cm^2$ followed by 4mS and 2mS, but few galls were at 6mS.

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Reduction of Bacterial Wilt Diseases with Eggplant Rootstock EG203-Grafted Tomatoes in the Field Trials (가지대목 EG203을 이용한 토마토 풋마름병 경감효과)

  • Lee, Mun Haeng;Kim, Ji Kwang;Lee, Hee Kyoung;Kim, Keyng Jae;Yu, Seung Hun;Kim, Young Shik;Lee, Youn Su
    • Research in Plant Disease
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    • v.19 no.2
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    • pp.108-113
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    • 2013
  • Wilt damage on tomato plants caused by Ralstonia solanacearum has been increased as the areas of tomato cultivation increased during the warm seasons. Also, the tomato rootstocks used to prevent the disease occurrence are not effective in the highly prevailing regions. Therefore, bacterial wilt resistant eggplant rootstock EG203, collected from AVRDC, was tested for its effect to deter the Ralstonia solanacearum wilt disease in the greenhouses at Buyeo Tomato Experiment Station from 2003 to 2005, and at Gumi, Kyungpook province from 2009-2011. Planting of eggplant rootstock EG203 should be done three weeks before the planting of tomato scions so that they can have similar stem diameter (2.5-3.0 mm) and can be easily grafted. Both insertion and inarching grafting showed 93-96% success rates. In the greenhouse tests at Buyeo Tomato Experiment Station from 2003 to 2005, eggplant rootstock EG203-grafted tomatoes showed the disease occurrence of 4.3%. On the other hand, non-grafted or other commercial rootstock-grafted tomatoes showed disease occurrence of 58.0% and 25.0-36.7%, respectively. In the greenhouse tests at Gumi, Kyungpook province in 2009, the disease occurrence on the EG203-grafted and non-grafted tomatoes was 2-5% and 20-80%, respectively. In 2010, at Gumi, Kyungpook province, when the wilt disease occurred slightly, the tomatoes grafted with tomato rootstocks B-blocking and Chung-gang, and eggplant rootstock EG203 showed similar disease severities, but EG203-grafted tomatoes formed lately cluster, resulting in the reduction of yield compared to tomato-grafted tomatoes. In 2011, at Gumi, Kyungpook province, when the wilt disease occurred severely, the tomato rootstocks 'B-blocking' and Chung-gang and eggplant rootstock EG203-grafted tomatoes showed disease occurrences of 60-85% and 0-1%, respectively. Therefore, it was concluded that tomato rootstocks 'B-blocking' and 'Chung-gang' are more useful in the areas contaminated with low levels of pathogen and eggplant rootstock EG203 is more useful in the areas contaminated with high levels of pathogen.

Inhibitory Effects of Extracts from Arabis glabra on Lipopolysaccharide Induced Nitric Oxide and Prostaglandin E2 Production in RAW264.7 Macrophages (RAW264.7 대식세포에서 장대나물 추출물의 Nitric oxide 및 Prostaglandin E2생성 저해효과)

  • Nam, Jung-Hwan;Seo, Jong-Taek;Kim, Yul-Ho;Kim, Ki-Deog;Yoo, Dong-Lim;Lee, Jong-Nam;Hong, Su-Young;Kim, Su-Jeong;Sohn, Hwang-Bae;Kim, Hyun-Sam;Kim, Bo-Sung;Shin, Ji-Sun;Lee, Kyung-Tea;Park, Hee-Jhun
    • Korean Journal of Plant Resources
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    • v.28 no.5
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    • pp.568-573
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    • 2015
  • Arabis glabra is a localized common rhizomatous flowering plant, This plant is often used in Korean traditional systems of medicine as a remedy for blood cleaning, detoxification, abscess, gastrospasm, arthritis, contraction and diarrhea. Generally drugs that are used for arthritis have antinociceptive and anti-inflammatory properties. However, validity of the anti-inflammatory activity has not been scientifically investigated so far. Therefore, the aim of this study was to investigate the anti-inflammatory potential of A. glabra using the ethanolic extract and its sub-fractions. To evaluate the anti-inflammatory effects, we examined the inflammatory mediators such as nitric oxide (NO) and prostaglandin E2 (PGE2) on RAW 264.7macrophages. Our results indicated that hexane and chloroform fraction significantly inhibited the LPS-induced NO and PGE2 production in the cells. The hexane fraction inhibitory activity for NO tests with IC50 values showed in 21.0 ㎍/㎖. The chloroform fraction inhibitory activity for PGE2 tests with IC50 values showed in 18.0 ㎍/㎖. These efficacy are expected to be able to present the potential for the development of health functional food for the prevention inflammatory diseases because it has sufficient preventive medical possibilities. Further, it is determined that it is necessary to further study the mechanism of cytokine and protein expression associated with inflammation.

Effects of Depth and Duration of Flooding on Growth and Yield at Flowering Stage in Tomato(Lycopersicon esculentum). (토마토(Lycopersicon esculentum)의 개화기 침수 처리에 따른 생육 반응)

  • Guh, Ja-Ock;Han, Sung-Uk;Kuk, Yong-In;Chon, Sang-Uk;Lee, Young-Man
    • Korean Journal of Environmental Agriculture
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    • v.16 no.2
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    • pp.130-135
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    • 1997
  • Tomatoes are flooded differently 0, 5, 10 and 15cm, according to the developing stages such as flowering stage under the condition of greenhouse. Along with this, they are treated according to the time condition such as 6, 12, 24, 48 and 120 hours. The results obtained are summarized as follows. Plant height decreased in the depth of $0{\sim}10cm$ for over 48 hours, in the depth of 15cm for over 24 hours. Number of leaves was the same as in control, and it decreased over. Number of flowers and fruit setting of individuals decreased conspicuously according as the depth and the hours got greater and longer. Adventitious root occurred remarkably in the depth of $0{\sim}10cm$, for over 24 hours and in the depth of 15cm, 12 hours. Epinastic curvature increased greatly as the depth and the hours got greater and longer. Diffusion resistance of stomata cell increased as the depth and the hours got greater and longer. Diseases occurred conspicuously as the hours of flooding got longer rather than as the depth greater. The preventing of diseases caused by insecticide was observed, but it was not greater than in the seedling and transplanting stage. Fertilization was effective in the case of increasing the weight of shoot. Number of fruits per plant did not decrease in the depth of 0cm up to 24 hours, but decreased on the deeper level of flooding and increased as the hours got longer. Moreover with the exception of 120 hours per respective depth of the treatment, average weight of a fruit got greater as the depth and the hours got greater and longer. In the case of epinastic curvature and diffusion resistance, there was negative correlation between all the other investigated characters and positive correlation between weight of a fruits and average weight of a fruit.

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The Incidence and Distribution of Viral Diseases in Barley Fields in Korea (국내 맥류재배지의 바이러스병 발생과 분포)

  • Park, Jong-Chul;Seo, Jae-Hwan;Choi, Min-Kyung;Lee, Kui-Jae;Kim, Hyung-Moo
    • Research in Plant Disease
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    • v.10 no.3
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    • pp.188-193
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    • 2004
  • The symptom expressions such as yellowish and mosaic spots in overwintering barley have been considered to be a damage by cold or water. However, it had revealed that the symptom expressions were caused by viruses throughout three year nationwide surveys. Barley yellow mosaic virus (BaYMV), Barley mild mosaic virus (BaMMV), and Soil-borne wheat mosaic virus (SBWMV) was detected in 2001-2003 and Barley yellow dwarf virus-MA V (BYDV -MA V) from field samples collected on March in 2003. The results of investigation showed that the incidence of BaYMV was more than 70% and that of BaMMV and SBWMV was 15.7-37.4% and 0.7-10.1 % in three year surveys, respectively. The incidence of BYDV-MAV was approximately 1 % in 2003 only. The distribution of BaYMV was relatively uniform throughout barley fields in Korea, but the incidence of the virus in Gyunggi Province was as low as 19% compared to 65-85% in the rest of regions. On the other hand, 70% of BaMMV was found to be in the west south regions of Korea, Jeonbuk and Jeonnam Provinces. Taken together, both BaYMV and BaMMV were thought to be dominant casual agents in overwintering barley by either single or mixed infections. Previous survey data for BaYMV from 1994 to 1996 indicated that the incidence of the virus was approximately 40% in Jeonbuk, Jeonnam, and Gyungnam Provinces. Thus, comparing with the results from the recent nationwide survey, the incidence of BaYMV had been rapidly increasing in overwintering barley fields in the southern part of Korea.

Occurrence of Viral Diseases in Barley Fields and Responses of Resistant Genes to BaYMV-Ik and BaMMV (맥류 바이러스병 발생 현황 및 BaYMV-Ik와 BnMMV에 대한 저항성 유전자의 반응)

  • Park Jong-Chul;Seo Jae-Hwan;Kim Yang-Kil;Kim Jung-Gon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.3
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    • pp.197-204
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    • 2005
  • The major symptom such as yellowish and mosaic spots in overwintering barley were mostly caused by viruses such as Barley yellow mosaic virus (BaYMV) and Barley mild mosaic virus (BaMMV) in the nation-wide for four years. The result showed that more than $78\%$ collected samples were infected by the viruses. The incidence of Ba YMV was more than $70\%$, and relatively uniformly distributed in the southern regions of barley fields in Korea. However the incidence of BaYMV in Gyeonggi Province was as low as $19\%$ compared to $65\~85\%$ in the rest of regions. Occurrence of BaMMV varied depending on investigated regions such as $20\~40\%$ in Jeonbuk, Jeonnam, Gangwon and Gyeongnam, and a lower infection in Gyeongbuk, Chungnam and Gyeonggi Provinces. In this result, $60\%$ of BaMMV was found to be in the southwest regions of Korea such as Jeonbuk and Jeonnam Provinces. Over all, both BaYMV and BaMMV were thought to be dominantly casual agents in overwintering barley by either solely or mixed infections. Soil-borne wheat mosaic virus(SBWMV) occurred at most $14\%$ in Gyeonggi and Barley yellow dwarf virus-MAY (BYDV­MAV) was found only one place in Jeonbuk, suggesting that SBWMV and BYDV-MAV were not significant diseases in Korea. Exotic genetic resources that possess different resistant genes to BaYMV and BaMMV were tested to identify the responses to the viruses occurred in Iksan. According to the ELISA results, BaYMV and BaMMV were infected in some plant materials but SBWMV was not identified. Any resistant gene was not effective to BaYMV-Ik (Insan strain) and BaMMY. Ishukushirazu (rym 3) and Chosen (rym 3), Tokushima Mochi Hadaka (rym 4y) and Hakei 1-41 (rym 5a) showed resistant response with little symptoms to BaYMY. The other five accessions possessing rym 1+5, rym 2, rym 4m, rym 5 and rym 9, respectively, were resistant to BaMMV. Various symptoms were observed in the tested plant materials such as not only yellowish and mosaic symptoms mostly but also necrotic spot, tissue necrosis, leaf stripe and leaf curling. However, it was difficult to find any relationship between resistant genes and specific symptoms.

Symptoms of Cucumber Virus Diseases Occurred in Sangju and Gurye in 2006 and 2007 (2006년과 2007년 상주와 구례에서 발생한 오이 바이러스병의 병징 특성)

  • Cho, Jeom-Deog;Lee, Jung-Hwan;Ko, Sug-Ju;Choi, Hong-Soo;Lee, Su-Heon;Choi, Gug-Seoun;Kim, Jeong-Soo
    • Research in Plant Disease
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    • v.17 no.2
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    • pp.196-204
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    • 2011
  • Virus diseases occurring on cucumber was surveyed at main plantation areas of 'Sangju' and 'Gurye' in 2006 and 2007. Viral infection rate on cucumber was ranged from 14% to 90% in fields and the average infection rate was 46% at Sangju area. Cucumber cultivated at Gurye area had viral incidence ranged 9-100% and averaged 48%. The majorly infected viruses were Cucumber green mottle mosaic virus, Zucchini yellow mosaic virus, Papaya ring spot virus and Watermelon mosaic virus-2, and their infection rates were 23.5%, 13.0%, 9.0% and 2.0%, respectively in 2006 and 2007. Mixed infection rate of duplex, triplex and tetraplex was 31.5%, 7.5% and 2.5%, respectively. The rate of infection type was 47.7% by single infection, 31.5% by double infection and 88.7% by both infection types. Important viruses involved in mixed infection types were Zucchini yellow mosaic virus, Cucumber green mottle mosaic virus, Papaya ring spot virus and Broad been wilt virus2, orderly. Symptom in single infection was almost same on 'Dadagi' line and 'Chicheong' line of cucumber cultivars. Cucumber green mottle mosaic virus produced various symptoms of chlorotic spot, vein chlorosis and vein wrinkle and so on. Main symptoms of vein chlorosis, and severe mosaic and malformation were induced by Zucchini yellow mosaic virus. Mild symptoms were occurred relatively by Papaya ring spot virus and Watermelon mosaic virus2.