DOI QR코드

DOI QR Code

Symptoms of Cucumber Virus Diseases Occurred in Sangju and Gurye in 2006 and 2007

2006년과 2007년 상주와 구례에서 발생한 오이 바이러스병의 병징 특성

  • Cho, Jeom-Deog (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, R.D.A.) ;
  • Lee, Jung-Hwan (Gyeongsangbukdo Agricultural Research & Extension Services) ;
  • Ko, Sug-Ju (Jeollanamdo Agricultural Research & Extension Services) ;
  • Choi, Hong-Soo (National Academy of Agricultural Science, R.D.A.) ;
  • Lee, Su-Heon (National Academy of Agricultural Science, R.D.A.) ;
  • Choi, Gug-Seoun (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, R.D.A.) ;
  • Kim, Jeong-Soo (National Academy of Agricultural Science, R.D.A.)
  • Received : 2011.06.09
  • Accepted : 2011.07.30
  • Published : 2011.08.31

Abstract

Virus diseases occurring on cucumber was surveyed at main plantation areas of 'Sangju' and 'Gurye' in 2006 and 2007. Viral infection rate on cucumber was ranged from 14% to 90% in fields and the average infection rate was 46% at Sangju area. Cucumber cultivated at Gurye area had viral incidence ranged 9-100% and averaged 48%. The majorly infected viruses were Cucumber green mottle mosaic virus, Zucchini yellow mosaic virus, Papaya ring spot virus and Watermelon mosaic virus-2, and their infection rates were 23.5%, 13.0%, 9.0% and 2.0%, respectively in 2006 and 2007. Mixed infection rate of duplex, triplex and tetraplex was 31.5%, 7.5% and 2.5%, respectively. The rate of infection type was 47.7% by single infection, 31.5% by double infection and 88.7% by both infection types. Important viruses involved in mixed infection types were Zucchini yellow mosaic virus, Cucumber green mottle mosaic virus, Papaya ring spot virus and Broad been wilt virus2, orderly. Symptom in single infection was almost same on 'Dadagi' line and 'Chicheong' line of cucumber cultivars. Cucumber green mottle mosaic virus produced various symptoms of chlorotic spot, vein chlorosis and vein wrinkle and so on. Main symptoms of vein chlorosis, and severe mosaic and malformation were induced by Zucchini yellow mosaic virus. Mild symptoms were occurred relatively by Papaya ring spot virus and Watermelon mosaic virus2.

오이 주요재배지인 상주와 구례에서 오이에 발생하는 바이러스병을 2006년과 2007년에 조사하였다. 경상북도 상주 지역 오이의 병징 발생률은 농가 포장에 따라서 14%에서 90%이었으며 평균 발생률은 46%이었다. 전라남도 구례 지역의 경우 농가 포장에 따라서 9%에서 100%이었으며 평균 발병률은 48%이었다. 주요 발생 바이러스는 CGMMV, ZYMV, PRSV와 WMV2의 4종류였다. 이러한 바이러스는 단독감염과 복합감염 형태로 발생하였다. 2006년과 2007년의 평균 감염률을 보면 단독감염의 경우 ZYMV가 23.5%로 가장 많이 발생하고 있었으며, PRSV가 13.0%, CGMMV가 9.0%, WMV2가 2.0% 순서이었다. 복합감염의 경우에는 2종 바이러스 복합감염이 31.5%, 3종 복합감염이 7.5%, 4종 복합감염이 2.0% 발생하였다. 오이에 발생하고 있는 바이러스 감염형태는 단독감염과 2종 복합감염이 각각 47.7%와 31.5%로 총 88.7%로 대부분을 차지하였다. 오이에 발생한 바이러스의 복합감염으로 많이 발생되는 바이러스로는 ZYMV, CGMMV, PRSV, BBWV2의 순서이었다. 단독감염된 오이의 병징은 다다기 계통이나 취청 계통 오이 모두 비슷한 병징이 나타났으나, CGMMV의 경우 퇴록 반점, 엽맥 퇴록, 엽맥 쭈그러짐 등 매우 다양한 병징이 나타났다. ZYMV는 엽맥 퇴록 병징을 위주로 심한 모자이크 및 기형 병징이 나타났다. 그 이외의 PRSV, WMV-2의 병징은 CGMMV와 ZYMV에 의한 병징에 속하는 비교적 약한 병징이었다.

Keywords

References

  1. Brunt, A. A., Crabtree, K., Dallwitz, M. J., Gibbs, A. J., Watson, L. and Zurcher, E. J. (eds.) (1996 onwards). Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20th August 1996. URL http://biology.anu.edu.au/Groups/MES/vide/
  2. Cho, J. D., Kim, J. S., Kim, J. Y., Kim, J. H., Lee, S. H., Choi, G. S., Kim, H. R. and Chung, B. N. 2005. Occurrence and symptoms of Tomato spotted wilt virus on vegetables in Korea. Res. Plant Dis. 11: 213-216. https://doi.org/10.5423/RPD.2005.11.2.213
  3. Cho, J. D., Lee, S. J., Chung, B. N., Kim, J. S. and Kim, T. S. 2006. Cucumber green mottle mosaic virus (CGMMV) reported newly on melon in Korea. Plant Pathol. J. 22: 426 (Abstract).
  4. Cho, J. D., Choi, H. S., Kim, J. S., La, Y. J. and Kim, K. S. 2002. Ultrastructural aspects of mixed infections with Turnip mosaic virus (AC18 and C5) and Ribgrass mosaic virus (CA1) in oriental cabbage. Plant Pathol. J. 18: 192-198. https://doi.org/10.5423/PPJ.2002.18.4.192
  5. Cho, J. D., Choi, H. S., Kim, J. S., Kim, G. H. and Kim, K. S. 2003. Symptom varience of mixed infections of six Turnip mosaic virus and one Ribgrass mosaic virus isolates in Crucifers. Plant Pathol. J. 19: 111-116. https://doi.org/10.5423/PPJ.2003.19.2.111
  6. Cho, J. D., Choi, H. S., Kim, J. S., Kim, G. H. and Kim, K. S. 2003. Ultrastructural differences in mixed infections of six Turnip mosaic virus and one Ribgrass mosaic virus isolates in Crucifers. Plant Pathol. J. 19: 117-122. https://doi.org/10.5423/PPJ.2003.19.2.117
  7. Cho, J. D., Kim, J. S., Choi, H. S., La, Y. J. and Kim, K. S. 2000. Ultrasturctural aspects of the mixed infections of Watermelon mosaic Potyvirus isolated from pumpkin and Cucumber green mottle mosaic tobamovirus from watermelon. Plant Pathol. J. 16: 216-221.
  8. Cho, J. D., Kim, J. S., Lee, S. H. and Chung, B. N. 2007. Triplex virion capture (VC)/RT-PCR for three seed transmissible tobamoviruses of CGMMV, ZGMMV and KGMMV occurring on Cucurbitaceae. Res. Plant Dis. 13: 82-87. https://doi.org/10.5423/RPD.2007.13.2.082
  9. Choi, G. S. 2001. Occurrence of two tobamovirus diseases in cucurbits and control measures in Korea. Plant Pathol. J. 17: 243-248.
  10. Jin, T. S., Kim, S. M., Ko, S. J., Lee, S. H. Choi, H. S., Park, J. W. and Cha, B. J. 2009. Occurrence of Papaya ringspot virus infecting cucurbit crops in Korea. Pesticide Science J. 13: 298-308.
  11. Kim, J. S., Cho, J. D., Choi, H. S. and Kim, K. S. 2000. Ultrastructural aspects of the mixed infections of Watermelon mosaic Potyvirus and Cucumber green mottle mosaic tobamovirus isolated from watermelon. Plant Pathol. J. 16: 211-215.
  12. Kim, M. K., Kwak, H. R., Kim, M. J., Kim, J. J., Ko, S. J., Lee, S. H., Kim, J. S., Kim, K. H., Cha, B. J. and Choi, H. S. 2008. Biological and molecular characterization of Squash mosaic virus isolated from melon in Korea. Plant Pathol. J. 24: 237 (Abstract).
  13. Ko, S. J., Lee, Y. H., Cha, K. H., Lee, S. H., Choi, H. S., Choi, Y. S., Lim, G. C. and Kim, K. H. 2006. Incidence and distribution of virus diseases on cucumber in Jeonnam province during 1999-2002. Plant Pathol. J. 2: 147-151. https://doi.org/10.5423/PPJ.2006.22.2.147
  14. Ko, S. J., Lee, Y. H., Lee, T. S., Yang, K. Y., Park, J. W. and Choi, H. S. 2004a. Influence of CGMMV infection times on growth and quality of watermelon and cucumber. Res. Plant Dis. 10: 48-52. https://doi.org/10.5423/RPD.2004.10.1.048
  15. Ko, S. J., Lee, Y. H., Cha, K. H., Park, J. W. and Choi, H. G. 2004b. Detection of CGMMV from commercial cucumber seed and resistance test of cultivars. Res. Plant Dis. 10: 154- 158. https://doi.org/10.5423/RPD.2004.10.2.154
  16. James, D. 1999. A simple and reliable protocol for the detection of apple stem grooving virus by RT-PCR and in a multiplex PCR assay. J. Virol. Meth. 83: 1-9. https://doi.org/10.1016/S0166-0934(99)00078-6
  17. Lee, J. H., Kim, Y. J., Lee, J. H., Park, S. J., Kim, M. K., Park, J. W., Choi, H. S., Lee, K. W., Kim, J. S., Moon, J. S. and Lee, S. H. 2007. Sequence variability of Zucchini yellow mosaic virus isolates from cucumber in Gyeongbuk province in Korea. Plant Pathol. J. 23: 374 (Abstract).
  18. Lee, S. H. 1981. Studies on various diseases occurring in various crops in Korea. Res. Rept. RDA. 23: 62-74.
  19. Lee, S. H., Lee, Y. G., Prak, J. W., Choi, H. S., Kim, Y. T., Cheon, J. U. and Lee, K. W. 2000. Nucleotide sequence of coat protein gene of Kyuri green mottle mosaic virus isolated from zucchini. Plant Pathol. J. 16: 118-124.
  20. Li, R. and Mock, R. 2005. An improved reverse transcriptionpolymerase chain reaction (RT-PCR) assay for the detection of two cherry flexiviruses in Prunus spp. J. Virol. Meth. 129: 162-169. https://doi.org/10.1016/j.jviromet.2005.05.019

Cited by

  1. 2007-2011 Characteristics of Plant Virus Infections on Crop Samples Submitted from Agricultural Places vol.18, pp.4, 2012, https://doi.org/10.5423/RPD.2012.18.4.277