• Journal of Bio-Environment Control
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• v.11 no.3
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• pp.144-148
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• 2002

The overall objective of this study was to determine which variety of carrot-10 Japan varieties or 6 domestic varieties- produces the optimum quality, when cultivated on Jeju Island. Plant height, leaf number and leaf weight were observed to be more superior in the purebred variety ‘Jungang’than other varieties. further studies, however, skewed the crossing variety ‘Bureoun’was superior to all others. Furthermore, when comparing root diameter, root length and root weight, the ‘Bibary’was superior in both the purebred and crossing variety, When researching Japan varieties, the crossing variety, ‘Jung’showed an increase in underground root. The purebred variety ‘Donghae’also showed an increase in underground root. Marketable yields in domestic varieties were greatest in ‘Hapa’, while in Japan varieties, ‘Kaneko’was the most productive. The percentage of root cracked was 15％ greater in ‘Bureon’. In the purebred variety, Jungang, the percentage was only 10％. The percentage of roots branched was highest in ‘Hidomi’which showed a rate of 9%, The roots branched rate was 2% for ‘Jung’and 6％ for‘Donghae’. Considering the percentage of roots clacked or branched, the marketability of carrots was higher in Japan varieties in comparison with domestic varieties. Therefore we conclude that, in the cropping patterns of carrots, the domestic variety has an advantage over the Japan variety.

• Journal of the Korean Institute of Landscape Architecture
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• v.42 no.1
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• pp.123-132
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• 2014

This study aims to develop the diagnostic evaluation method of the riverine ecobelt for construction, conservation, and maintenance of the riverine ecobelt. The value indices in the proposed evaluation method are composed of total 5 fields and 19 elements. The 5 fields are flood control, environmental function, growth of plants, ecobelt function, and restoration potential. Flood control field is composed of total 3 elements such as length, width, and density of green area. Environmental function field is composed of 4 elements such as park use, landscape boundary and edge, microclimate control, non-point pollution control. Growth of plants field is composed of 6 elements such as species composition, forest height, stratum structure, vine plants, plant vitality, and succession of plants. Ecobelt function field is composed of 4 elements such as longitudinal connectivity, lateral connectivity, in-stream forest or habitat, roads on bank top. Restoration potential field is composed of 2 elements such as landform and land use of the immediate vicinity. The score system ranging 1~4 was adopted. The weighting parameters of elements were unified with each other. The final grade system ranging 1~5(1: very good~5: very bad) was adopted, and the final grade was evaluated by the mean values of each field. According to the test application of the diagnostic evaluation method of the riverine ecobelt, the final grades showed effectively the real condition of each site.

• Korean Journal of Soil Science and Fertilizer
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• v.23 no.2
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• pp.152-159
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• 1990

Four active strains, producing siderophore and antagonizing to soil plant disease fungi, were isolated and identified from the wheat rhizosphere to reduce the injurious effects of continuous cropping and study on the biological control. The obtained results were summerized as follow. 1. Four strains of fluorescent Pseudomonas were isolated from the wheat rhizosphere and identified as Pseudomonas fluorescens Biotype A(Ps-1,2,5) and Biotype B(Ps-3) That strains inhibitied growth of R. solani, F.oxysperum and F. solani in vitro 2. Optical density of pigment was maximum at 410nm. 3. Siderophore production by identified strains was decreased with addition of $Fe^{+3}$, although not decreased with addition of $Fe^{+2}$ 4. Pigment of Ps-1, 2 and 3 strain inhibitied growth of R. slani, F. oxysperum and F. solani but pigment of Ps-5 strain did not inhibitie growth of R. solani 5. Effect of inoculation was in order of Ps-2, 1, 5 and 3 strain through the dark culture method, and effect of Ps-1 and Ps-2 was greater than that of treatment of captan 50ppm.

• Korean Journal of Soil Science and Fertilizer
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• v.25 no.4
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• pp.387-393
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• 1992

The fate of inoculum strain of Bradyrhizobium japonicum was studied by using genetically marked strain. RJB6 $str^rnal^rneo^r$. A spontaneous mutant of B. japonicum isolated from nodules was made to have antibiotic resistance against streptomycin and nalidixic acid. In order to make genetically marked strain, neomycine resistant gene(Tn5) was introduced into this spontaneous mutant by conjugation with E. coli containing pSUP2021. The southern hybridization was carried out to confirm the plasmid insertion. Hybridization of chromosome DNA using pSUP2021(Tn5) as a probe showed that Tn5 was located on the 4.9kb fragment of chromosome. Soybean seeds were planted into a soil previously cultivated with soybean and inoculated with different cell densities of marked strain. Fourty days after planting, the inoculation effects on nodule number, nodule fresh weight, plant height and nitrogen content in the plot inoculated with heavy cell suspension was a little better than those in the plot with low inoculation. The recovery percentage of the marked strains was about 12% in the plot inoculated with heavy density cell suspension, while 5% in the plot inoculated with low cell suspension.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.4
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• pp.467-474
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• 2012

This study was carried out to evaluate effect of temperature condition on nitrogen mineralization of organic matter, distribution of microbial group by PLFA profiles, and soil microbial community structure in volcanic ash soil. Dried soil 30 g mixed well each 2 g of pellet (OFPE) organic fertilizers, pig manure compost (PMC), and food waste compost (FWC). And then had incubated at $10^{\circ}C$, $20^{\circ}C$, and $30^{\circ}C$, respectively. Nitrogen mineralization rate increased with increasing temperature and that was in the order of FWC>OFPE>PMC. Distribution ratio of microbial group by PLFA profiles were different significantly caused by incubation temperature and the type of organic matter. As incubating time passed, density of microbial group decreased gradually. The Gram-bacteria PLFA/Gram+ bacteria PLFA, Fungi PLFA/Bacteria PLFA, and Unsaturated PLFA/saturated PLFA ratios were decreased according to the increasing temperature gradually. But cy19:0/$18:1{\omega}7c$ ratio increased both FWC and PMC treatment. Principal component analysis using PLFA profiles showed that microbial community structure made up clearly at both 75 days ($10^{\circ}C$) and 270 days ($30^{\circ}C$) by temperature factor. As incubating time passed, microbial community structure shifted gradually.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.6
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• pp.1017-1021
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• 2012

A field experiment was conducted to evaluate effect of water soluble silicate fertilizer (WSS) application on rice plants with respect to comparing with powdery slag-originated silicate fertilizer (PSS) and granular one (GSS). The 30-day seedlings were transplanted on May 10, 2012. The plot size was $25m^2$, and the planting density was 15 hills $m^{-2}$. The standard application level was $2kg\;ha^{-1}$ for WSS, $200kg\;ha^{-1}$ for GSS, $200kg\;ha^{-1}$ for GSS. The application rates were 50 %, 100 %, and 200 % of the standard levels. The soil and plant samples were taken after harvest on September 10. Strength weight of the stem was measured on the center of the 5-cm of the fourth internode. Nutrient contents and yield of grains and were evaluated. The strength weight of the stem was positively correlated with the silicate content of the stem with the highly significant $R^2$ of 0.601. The strength of the stem was satisfactorily enforced by application of 50 % WSS and GSS, and 100 % PSS. Application of 50 % or 100 % of WSS showed little difference in rice yield in comparison with application of 100 % of PSS or GSS. Therefore, application of $20kg\;ha^{-1}$ of WSS would be recommendable for rice cultivation which enforced stem strength, and increased yield of rice.

• Horticultural Science & Technology
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• v.28 no.4
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• pp.552-560
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• 2010

$GA_{4+7}$+BA, a plant growth regulator for induction of feathering in young apple tree and increasing fruit size, was applied by various methods on 'Gala'/M.9 apple trees in high density orchard for 4 years to investigate its effect on fruit and shoot growth. $GA_{4+7}$+BA($80-300mg{\cdot}L^{-1}$) increased fruit length, fruit weight, and L/D ratio regardless of application methods, but it did not affect soluble solid content, acidity, leaf area, and chlorophyll. Seed number was not affected by $GA_{4+7}$+BA application, however, more immature seeds was observed in treated 'Gala' fruit. Shoot growth was increased when spraying $GA_{4+7}$+BA at tree crown but not affected when spraying at fruit directly. More significant fruit growth was observed when $GA_{4+7}$+BA was applied on the fruits between late of May and early of June when fruit cell division ended; however, high concentration of $GA_{4+7}$+BA resulted in lower fruit storability because of lower firmness. Hence, more attention should be paid when applying high concentration of $GA_{4+7}$+BA to small sized fruit cultivars like 'Gala'.

• Microbiology and Biotechnology Letters
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• v.37 no.1
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• pp.1-9
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• 2009

The genetic monitoring method was developed for the rapid detection of the PGPR and biocontrol agent, B. subtilis AH18 in red-pepper field soil by multiplex PCR using sid, aec and cel gene primers. The monitoring of B. subtilis AH18 in the soil was carried by amplified a 2,3-dihydro-2,3-dihydroxy benzoate dehydrogenase [EC: 1. 3. 1. 28]gene (sid - 794 bp : EF408238) which is a key enzyme of siderophore synthesis, an auxin efflux carrier gene (aec - 1,052 bp : EF408239) and a cellulase gene (cel - 1,582 bp : EF070194). The natural un sterilized soil was inoculated with B. subtilis AH18 to determine the sensitivity ($1.8\times10^5$ cfu/g) of multiplex PCR for the rapid dectection and then the strain was monitored successfully in rhizosphere or non-rhizosphere soil of red-pepper cultural soil. At 3 weeks after the treatment, density of the strain was monitored more abundantly in rhizosphere soil.

• Korean Journal of Environmental Agriculture
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• v.17 no.3
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• pp.254-259
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• 1998

This study was focused on finding out the potential problems associated with organic farming system. The effect of composted animal manures subsequent inoculation of microbes on growth and yield of tomatoes (Lycopersicon esculentum Mill. 'Minicarol') were examined to develop a proper organic farming practice. Plant heights were greater in composted manure treatment than in conventional practice, whereas widths of leaves were higher in conventional field. Chlorophyll contents between various amount of composted manure application were gradually decreased and showed no significant differences after 45 days of planting. The yield in the treatment applied 12 ton of composted animal manure per 10a as pre-planting fertilizer and following microbial inoculation were only 50-60% compared to that of conventional farming. However, yield increased up to 80% when additional composts were applied to the treatment received 6 ton of composted animal manure per 10a in the middle of cultivation. Microbial inoculation followed by composted manure application induced rapid decrease of nitrogen content in soil. However, the density of microorganisms was significantly increased. Tomatoes produced through organic farming were clear in color, Further, soluble solid and acid content were increased. The highest level of acid and solids were observed in the treatment applied 12 ton of digested swine manure per 10a. Although nitrogen content including ammonium and nitrate rapidly increased after application of composts, these were significantly reduced approximately 4-5 weeks after planting. The level of phosphorus, potassium, magnesium and calcium showed gradual decrease compared to nitrogen.

• Horticultural Science & Technology
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• v.29 no.6
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• pp.617-622
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• 2011

The study was conducted to determine the efficiency in producing spontaneous polyploids in some mandarin hybrids with different seed embryony. Seed formation by open pollination, frequency of spontaneous polyploids, and plant growth characteristics were evaluated in four mandarin hybrids with polyembryony such as 'Amakusa', 'Haruka', 'Hayaka', and 'Seminole' and two with monoembryony such as 'Benibae' and 'Harehime'. The mean number of the developed seeds per fruit was 10.0 and frequency of small seeds was 25.1%. Polyploids were selected from plants germinated in vitro by a flow cytometry and confirmed by chromosome analysis. One triploid was produced from 'Harehime', one tetraploid, 'Amakusa', and one tetrapoid, 'Benibae'. There were little differences in leaf shape, thickness, petiole length, and internode length between diploids and polyploids such as tri- or tetraploid. However, polyploids had larger stomata and lower density of stomata in abaxial epidermis than diploids. SPAS indicating chlorophyll content and photosynthetic rate were significantly affected by ploidy level. The results indicated that spontaneous polyploids might be produced by open pollination in some mandarin hybrids and monoembryony had higher frequency in polyploid occurrence than polyembryony.