• Title/Summary/Keyword: Plant Leaf Disease

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Changes of Bacterial Diversity Depend on the Spoilage of Fresh Vegetables (신선 채소류의 부패에 따른 세균의 다양성 변화 및 세균에 의한 채소 부패 조사)

  • Lee, Dong-Hwan;Ryu, Jung-El;Park, So-Yeon;Roh, Eun-Jung;Oh, Chang-Sik;Jung, Kyu-Suk;Yoon, Jong-Chul;Heu, Sung-Gi
    • Research in Plant Disease
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    • v.17 no.1
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    • pp.38-43
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    • 2011
  • Almost 10~30% of vegetables were discarded by the spoilage from farms to tables. After harvest, vegetables are often spoiled by a wide variety of microorganisms including many bacterial and fungal species. This investigation was conducted to extent the knowledge of relationship the spoilage of vegetables and the diversity of microbes. The total aerobic bacterial numbers in fresh lettuce, perilla leaf, and chicory were $2.6{\sim}2.7{\times}10^6$, $4.6{\times}10^5$, $1.2{\times}10^6\;CFU/g$ of fresh weight, respectively. The most common bacterial species were Pseudomonas spp., Alysiella spp., and Burkholderia spp., and other 18 more genera were involved in. After one week of incubation of those vegetables at $28^{\circ}C$, the microbial diversity had been changed. The total aerobic bacterial numbers increased to $1.1{\sim}4.6{\times}10^8$, $4.9{\times}10^7$, and $7.6{\times}10^8\;CFU/g$ of fresh weight for lettuce, perilla leaf, and chicory that is about $10^2$ times increased bacterial numbers than that before spoilage. However, the diversity of microbes isolated had been simplified and fewer bacterial species had been isolated. The most bacterial population (~48%) was taken up by Pseudomonas spp., and followed by Arthrobacter spp. and Bacillus spp. The spoilage activity of individual bacterial isolates had been tested using axenic lettuce plants. Among tested isolates, Pseudomonas fluorescence and Pantoea agglomerans caused severe spoilage on lettuce.

The City Rhinoreaction Research of the Corn Feed for the Heavy Metal Removal of the Pig Ordure Sludge Using the Citric Acid and Stability Evaluation (구연산을 이용한 돈분슬러지의 중금속 제거 및 안정성평가를 위한 사료용 옥수수의 시비반응 연구)

  • Oh, Tae-Seok;Kim, Chang-Ho;Choi, Bong-Su
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.31 no.4
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    • pp.395-408
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    • 2011
  • The study which it sees exclusions the copper and the zinc which contain in pig sludge, It study pig sludge resources fertilizer production which are rational, pig sludge resources fertilizers after seeding, silage corn it investigates growth characteristics and forage value, the result which investigates pig sludge resources fertilizers effectiveness with afterwards is same. With fertilizer ingredients in pig sludge chemical qualities, the content of the nitrogen and the phosphoric acid comes 4.4% to be 6.29%, pH 7.02 and content of the copper and the zinc which is a heavy metal which contains in pig sludge with 805 mg/kg and 1,704 mg/kg, it is a restrictive standard of the fertilizer, 300 mg/kg and 900 mg/kg it sees to be high, it manufactures citric acid 1 hydrate with the organic acid solution, heavy metals of pig sludge where it is a mixture ratio of the organic acid solution, it divides to 25%, 50%, 75% and 100% 4 kind levels, the result which measures the heavy metal exclusion ratio of the copper and the zinc, the mixture ratio of the organic acid solution to be many exclusion ratio of the copper and the zinc is showing a just interrelation, from organic acid solution 100% level content of pig sludge remains copper and zinc 330.03 mg/kg and 41.28 mg/kg, it shows the exclusion ratio of copper 59% zinc 97%. 'Cheonganok' growth characteristics with citric acid 1 hydrate, Treatment 2 and control growth characteristics etc, it exclusion the copper and the zinc it doesn't appear on significant difference statistically but, treatment 3 after only pig sludge in resources disposal where it seeding, growth characteristics of leaf area etc. is badness, it compares in control and treatment 2 the growth characteristics badness, it is appearing, it is caused by with disease and insects occurrence of $Ostrinia$ $furnacalis$ and brown spot, the damage was many. From forage value, Treatment 2 where it exclusion the heavy metal with the citric acid 1 hydrate with control it compares and there are not significant difference from crude protein and ADF and NDF contents etc., seeding only Pig Sludge in resources disposal treatment 3, it is caused by with $Ostrinia$ $furnacalis$ etc., trunk and aging of the leaf to be high ADF content is low. but from crude protein, the nitrogen ingredient which pig sludge has and interrelation it seemed and high numerical value were confirmed.

Studies on the Growth Characteristics, Forage Yields and Nutritive Values of Heading and Headless Types of Sorghum X Sudangrass Hybrids (출수 및 비출수형 Sorghum X Sudangrass 교잡종의 품종별 생육특성, 생산성 및 사료가치에 관한 연구)

  • 임영철;성병렬;최기준;임용우;김기용;임근발;박근제
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.22 no.3
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    • pp.213-220
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    • 2002
  • The growth characteristics, forage yields and nutritive values of heading and headless types of sorghum $\times$ sudangrass(Sorghum bicolar L.) hybrids were examined to evaluate the adaptability of new cultivars at Suwon and Chunahn for two years from 2000 to 2001 in a randomized complete block design with three replications. Eleven cultivars of heading type and 4 cultivars of headless type were used in this study. The dry matter(DM) yield of AG 2501 was highest in the heading type cultivars, and PAC F8350 in the headless type cultivars, respectively. The DM yields of heading types were slightly higher than those of headless types but there were not significantly different. Early growth and regrowth characteristics of heading type was highest in AG 2501 and GW 9110G, respectively. GW 9110G and DMD were early heading types, and AG 2501 and GW 9161F were middle heading types. AG 2501 was longest and GW 9161F was shortest in plant height. Cultivars with long leaf length were appeared to be larger fur leaf width and stem diameter. Brix content was highest in DMD as 9.7%, and disease and insect resistances were not significantly different among the heading types. Early growth of headless type cultivar, PAC F8350 was slightly lower than that of Jumbo but stronger in regrowth, wider in leaf width and stem diameter, and stronger in lodging. Comparisons of growth characters between heading and headless types of cultivars were not easy in general but between them, brix contents of heading types ranging from 6.7 to 7.9% were greatly higher than those of headless types ranging from 3.6 to 3.9%. Nutritive values such as crude protein, ADF, NDF and digestibility of headless type cultivars were higher than those of heading types. In conclusion, of heading type cultivars, GW 9110G, AG 2501, DMD and GW 9161F, and of headless type cultivars, Jumbo and PAC F8350 were recommended as high yielding cultivars with good adaptability in domestic environment.

A New Short Growth-Duration Rice Cultivar, "Keumo 3" (소득작물 전후작용 단기성 벼 품종 "금오3호")

  • Kang, Jong-Rae;Lee, Jong-Hee;Kwack, Do-Yeon;Lee, Jeom-Sik;Park, No-Bong;Ha, Woon-Gu;Park, Dong-Soo;Yeo, Un-Sang;Lim, Sang-Jong;Oh, Byeong-Geun
    • Korean Journal of Breeding Science
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    • v.41 no.3
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    • pp.292-298
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    • 2009
  • A new rice cultivar "Keumo 3" was developed for adopting under double cropping system with after or before cash crop cultivation. It was selected from the cross-combination between YR17202 $F_2$/Shinkeumobyeo//YR15727-B-B-B-102. The parent, YR17202 $F_2$ individual plant, was used for tolerance to lodging, it derived from a cross between Nonganbyeo/Shinkeumobyeo. Nonganbyeo is well known to lodging tolerance cultivar, as well as biotic stress, because it was developed by crossing with Tongil type. And the YR15727-B-B-B-102 line used as another parent with short growth duration, likewise highly resistance to rice blast disease. The pedigree derived from the cross-combination YR17202 $F_2$/Shinkeumobyeo//YR15727-B-B-B-102 were generated to $F_7$, and a best line among them named as Milyang 201. After a series of yield trials, including local adaptability test conducted throughout the peninsular of Korea, Milyang 201 was registered with the name of "Keumo 3" in 2005. The cultivar belongs to a early maturing group and heads 4 days earlier than Keumobyeo, a standard cultivar. It has short culm, and less spikelet number per panicle than Keumobyeo. However, its milled rice yield grown under extremely late transplanting time, 10. July, over the 3 local sites for 2003-2005 years, averaged 4,48 MT/ha, which is 6% higher than the standard, Keumobyeo. "Keumo 3" has showed a durable resistance to leaf blast disease during fourteen blast nurseries screening covered from south to north in Korea for 2003-2007 years. And it was confirmed harbours pi-zt, a durable blast resistance gene. Moreover it was incompatible with 19 blast isolates under artificial inoculation, except one isolate, K1101. Additionally, "Keumo 3" exhibits resistance to $K_1$, $K_2$ and $K_3$ of bacterial blight pathogen, as well as strip virus disease resistance, and moderate resistance to dwarf virus disease. Consequently, the new rice cultivar "Keumo 3" would be well adopted where a bio stress makes a big problem annually.

Effect of Foliar Sprays of CaCl2 for Improving Fruit Quality of (복숭아 )

  • Kim, Ik-Youl;Kim, Mi-Young;Ru, Jong-Ho;Kim, Min;Lee, Yong-Se;Chang, Tae-Hyun
    • Korean Journal of Environmental Agriculture
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    • v.25 no.3
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    • pp.276-283
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    • 2006
  • To evaluate the effectiveness of the foliar spray of calcium on "Baekdo" peach fruit, we carried out experiments in the orchards. The sprays were applied with $CaCl_2\;(Ca:\;400mg.kg^{-1})\;and\;CaCl_2$ with adjuvants (amino acid, $2g.kg^{-1}$; phytic acid, $2ml.kg^{-1}$ and wood vinegar, $2ml.kg^{-1}$) for four times from June 12 through July 4 at weekly intervals. The fruits and leaves were evaluated for Ca content, firmness and incidence of Brown rot caused by Monilinia fructicola at harvest To evaluate fruit quality included Ca content, firmness natural decay during the storage, the fruits were stored at room temperature for 14 days. The Ca content in leaf and fruit flesh at harvest was significantly increased (P=0.05) in $CaCl_2$ + amino acid treatment among $CaCl_2$ treatments. However, there was not significant Ca content in fruit peel. The firmness of flesh increased significantly (P=0.05) in $CaCl_2$ + amino acid treatment. The natural decay (Rhizopus stolonifers) during storage at the room temperature for 14 days, the fruit treated with $CaCl_2$ + wood vinegar exhibited lowest (P=0.05) incidence. Also, the firmness of the fruit during storage was firmer with treated $CaCl_2$ than untreated fruit. In the treatments of $CaCl_2$ + phytic acid and $CaCl_2$ + amino acid, it was possible to reduce incidence of Brown rot caused by M. fructicola most effectively in the field. In addition, inoculation with M. fructicola in fruits was also the most effective treatment for inhibiting disease development in vitro. These results suggested that the foliar spray of $CaCl_2$ with adjuvants increased the content of Ca and firmness of the fruits positively. It also inhibited the natural decay and the Brown rot effectively.

Controlling activity of Bion-M against bacterial spot caused by Xanthomonas euvesicatoria (고추 세균점무늬병에 대한 Bion-M의 방제 효과)

  • Kim, A-Hyeong;Yeon, Cho-Long;Kim, Joo-Hyung;Kim, Heung-Tae
    • The Korean Journal of Pesticide Science
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    • v.16 no.2
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    • pp.171-177
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    • 2012
  • Pepper seedlings of 6-leaf stage were inoculated with cell suspension of Xanthomonas euvesicatoria 1523 7 days after the application of Bion-M by soil-drenching. Disease severity in the treatment with 20 ${\mu}g\;mL^{-1}$ of acibenzolar-S-methyl, with which Bion-M was composed, was 19%, whereas that of the untreated control was 75%. The resulting control value of acibenzolar-S-methyl was calaulated as much as 74.7%. The control value of acibenzolar-S-methyl was dependent with the applied concentrations and ranged 29.3% to 49.3% at 4.0 and 0.8 ${\mu}g\;mL^{-1}$ of acibenzolar-S-methyl, respectively. Phytotoxicity was observed at 20.0 ${\mu}g\;mL^{-1}$, as lower leaves became to be yellowed and defoliated. The cell density of inoculum suspension of X. euvesicatoria 1523 affected the control value of acibenzolar-S-methyl. With optical density (O.D.) of 0.5 the control value of 4.0 ${\mu}g\;mL^{-1}$ of acibenzolar-S-methyl was 86.0%. However, the control value improved as high as 97.8% at the O.D. value of 0.1. The control value was 75.0% in adult plant of pepper, when acibenzolar-S-methyl was treated by soil-drenching 7 days before inoculation with cell suspension of X. euvesicatoria 1523. The control effect of acibenzolar-S-methyl on pepper bacterial spot was obtained in pepper field, showing that the control value at 10.0 ${\mu}g\;mL^{-1}$ was 71.2%.

Fundamental Study on the Evapo- transaration Requirements of Patty rice Plant (벼 용수량계획상의 엽면증발량 및 주간수면 증발량에 관한 기초적인 연구)

  • 김철기
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.11 no.2
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    • pp.1651-1660
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    • 1969
  • The purpose of this study is to find out the reasonable amount of evapo-transpiration required for the paddy rice plant during the whole growing season. So. On the basis of the experimental data concerning the evapo-transpiration form 1966 to 1968, the author obtained the follow results. 1) The leaf area index in the densely planted plo is generally higher than that in the conventionally planted one during the first half of growing season So, the coefficient of transpiration in the former plot is somewaht higher than in the latter, and the coefficient of water surface evaporation under the plant cover has the inverse relation between both plots. 2) It is unreasonable that coefficient of evapo-transpiration is applied to the calculation of the evapo-transpiration requirements of each growing stage, because a degree of variation in meteorological factors and in the thickness of the plant growth is involved in it. 3) It is most reasonable that the rate of transpiration and of the water surface evapoation is applied to the calculation of the transpirated amount and evaporated one in each growing stage, because it shows almost constant value in spite of any meteorological conditions in so far as the variety of rice, planted density and control of applying fertilizer are sanme and the disease and blight are negligible. 4) The ratio of the amount of transpiration to the weight of the whole air dried yields has the tendency of decreasing as that of the yields increases having almost constant value despite the amount of pan evaporation; and the value is about 210 when the weight of root parts is included to that the yields. 5) Although the required amount of transpiration during the whole growing season can be calculated with the above ratio, Fig. 7 showing the relation between the amount of transpiration and the weight of the yields is more reasonable and will be convinient to find it. And the requirements of water surface evaporation during the same season can also be directly found witht theweight air dried straw refering to Fig. 8.

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Studies on the Improvement of Nursery for Better Ripening Percentage and Prevention of Red Discoloration of Rice Variety "Tongil" (통일벼의 등숙(登熟) 향상(向上)과 적고방지(赤枯防止)를 위(爲)한 묘대개선(苗垈改善)에 관(關)한 연구(硏究))

  • Choi, Boum Rawl
    • Korean Journal of Agricultural Science
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    • v.1 no.1
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    • pp.9-26
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    • 1974
  • 1. Results in Nursery This experiment was carried out on the effect of the seed treament, soil preparations, kinds of covering soil and inside covering methods in two rice varieties, 'Tongil' and 'Akibare' to find out the most reasonable model of the flat nursery bed, with which lower cost is required comparing with the tunnel nursery. The results obtained are as follows: (1) The seedling of all plots of the ordinary seed were very poor compared to the plots of sprouted seed. (2) In case that the variety 'Tongil' was cultivated on the dry nursery bed, the good seedling percentage and the plant height rat io were significantly increased but the other characteristics of the seedling were not noticeable. (3) The kinds of the covering soil had not an effect on the seedling growth significantly. (4) Inside straw mulching was seemed effective for the protection in the case of the extreme high temperature and heavy rain fall, even though there was not significant differences between inside straw mulching and no treatments at the flat type nursery. (5) Difference of seedling growth between the flat type nursery and the tunnel type nursery was not significant. And it's reason was thought that the covering period of polyethylene film was short in semi hot nursery for the common early transplanting cultivation of rice. (6) The percentage of good seedling was higher at 'Akibare' than 'Tongil', variety but the number of seedling leaf and the seedling growth ratio in height were significantly increased in the variety 'Tongil'. The other seedling characters between there two varieties were not significantly different. 2. Results after transplanting This experiment was conducted to study on the ripening percentage, rice yield and disease, appearance of the seedling from sprouted seed plots including common irrigated nursery as check plot after transplantnig. The results obtained are summarized as follows: (1) The rice yield, the yield components and the appearance of leaf discoloration of both varieties, 'Tongil' and 'Akibare' were slightly betterat the plot of the standard tunnel nursery than that of the flat nursery with inside mulching or the among these three plots. (2) For 'Tongil' variety, the ripening percentage and the rice yield were significantly decreased at the common irrigated nursery compared with semi hot nursery. (3) The ripening percentage and the rice yield of 'Akibare' contrasted with 'Tongil' were significantly decreased at thesemi 'hot-nursery compared with common irrigated nursery. The main reason was thought to be the injury of the rice stripe disease (Rice stripe disease virus). Considering above mentioned experimental result, the seedling of 'Tongil' must be cultivated on the semi bot nursery for better ripening percentage as well as rice yield and for prevention of red discoloration. And as a model of semi hot nursery, the polyethylene covering nursery of standard tunnel type is most desirable but that of flat type with inside straw mulching is thought to be desirable too.

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Resistance Degree of Cucurbits Cultivars to Colletotrichum orbiculare (탄저병(Colletotrichum orbiculare)에 대한 박과작물의 저항성)

  • Shim, Sun-Ah;Jang, Kyoung Soo;Choi, Yong Ho;Kim, Jin-Cheol;Kim, Heung Tae;Choi, Gyung Ja
    • Horticultural Science & Technology
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    • v.31 no.3
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    • pp.371-379
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    • 2013
  • Anthracnose disease caused by Colletotrichum orbiculare, induces severe damage to cucurbits worldwide. Resistance of 112 commercial cultivars of cucurbits to C. orbiculare was evaluated. Seedlings of each cultivar at 2- to 3-leaf stage were inoculated with C. orbiculare KACC 40809 by spraying spore suspension of the fungus at a concentration of $4.0{\times}10^5$ spores/mL. Among the 36 cultivars of cucumber, 'Asiastrike', 'Tongilbaedadagi', 'Daeseon', 'Cheongrokmatjjang', 'Nokyacheongcheong', and 'Asianogak' were moderately resistant and the others were susceptible. All the tested cultivars of melon (33) and watermelon (4) showed highly susceptible response to C. orbiculare. On the other hand, the squash cultivars (17) represented less susceptibility to the fungus than the other cucurbits. Of the squash cultivars, 'Gammirak' and 'Teotbat' were resistant and 12 cultivars were moderately resistant. Among the rootstocks for cucurbits, ten cultivars including 'JjeuyakaEX', 'Nunbusyeo', 'Union', 'RS111', 'Ganggeuntoza', 'Hwangjaetoza', 'NO.8', 'Shintoza', 'Bulpaetoza', and 'Newtype' showed high resistance to the anthracnose pathogen. From the results, the resistant cultivars could be used as sources of resistance to cucurbits anthracnose (C. orbiculare) in the future breeding programs.

Growth Characteristics and Productivity of New Orchardgrass(Dactylis glomerata L.) Variety "Kordione" (오차드그라스 신품종 "코디원"의 생육특성과 수량성)

  • Choi, Gi-Jun;Sung, Byung-Ryul;Seo, Sung;Kim, Ki-Yong;Lee, Joung-Kyong;Park, Hyung-Soo;Moon, Chung-Sup;Ji, Hee-Chung
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.27 no.1
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    • pp.53-56
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    • 2007
  • A new orchardgrass (Dactylis glomerata L.) variety named "Kordione" has developed by the National Livestock Research Institute (NLRI) in 2005. To develope a new variety of orchardgrass, 5 superior clones were selected and polycrossed for seed production. Various agronomic growth paramaters and forage production of "Kordione" were examined at Suwon from 2001 to 2002. During 2003-2005, regional trials were conducted at Suwon, Pyungchang, Youngju, Iksan, and Jeju. "Kordione" showed a medium type growth habit in fall and spring, and short in length of flag leaf and upper internode. Plant height of "Kordione" was similar to that of standard variety, "Ambassador" and heading date was 2 days later than 10th May compared to Ambassador. "Kordione" showed enhanced winter hardiness, lodging tolerance and disease resistance compared to Ambassador. In addition, dry matter yield (12,081 kg/ha) of "Kordione" was also higher (11%) than that of the Amabassador. However, nutritive value was similar in both varieties.