Browse > Article
http://dx.doi.org/10.5338/KJEA.2006.25.1.007

Effect of Elevated Ultraviolet-B Radiation on Yield and Differential Expression of Proteome in Perilla (perilla frutescens L.)  

Hong, Seung-Chang (National Institute of Agricultural Science and Technology, Plant Nutrition Division, RDA)
Hwang, Seon-Woong (National Institute of Highland Agriculture, Environment Management Division, RDA)
Chang, An-Cheol (National Institute of Agricultural Science and Technology, Plant Nutrition Division, RDA)
Shin, Pyung-Gyun (National Institute of Agricultural Science and Technology, Plant Nutrition Division, RDA)
Jang, Byoung-Choon (National Institute of Agricultural Science and Technology, Plant Nutrition Division, RDA)
Lee, Chul-Won (Department of Crop science, College of Agriculture, Life & Environment Sciences, Chungbuk National University)
Publication Information
Korean Journal of Environmental Agriculture / v.25, no.1, 2006 , pp. 7-13 More about this Journal
Abstract
Plastichouse cultivation for crops and vegetables in the winter has been widely popularized in Korea. In the vinylhouse Ultraviolet B penetration is lower than in the field, and so some problems, as plant overgrowth and outbreak of disease, occurred frequently. The effect of artificial supplement ultraviolet B $(UV-B:280{\sim}320nm)$ radiation on the physiological responses and yield of perilla (perilla frutescens) was investigated UV-B ray was radiated on perilla with the 10th leaf stage at the distance of 90, 120 and 150 cm from the plant canopy for 30 days after planting in the vinylhouse. The production of fresh perilla leaves was high in the order of plastic house, ambient+50% of supplemental UV-B, ambient ambient+100% of supplemental UV-B. Enhanced UV-B radiation affected the intensity of thirty-three proteins in 2-dimensional electrophoretic analysis of proteins and ten proteins out of them seemed to be responsive to UV-B : a protein was, ATP synthase CF1 alpha chain, down regulated and nine proteins (Chlorophyll a/b bindng protein type I, Chlorophyll a/b binding protein type II precursor, Photosystem I P700 chlorophyll a apoprotein A2, DNA recombination and repair protein recF, Galactinol synthase, S-adenosyl-L-methionine, Heat shock protein 21, Calcium-dependent protein kinase(CDPK)-like, Catalase) were up-regulated.
Keywords
Perilla; UV-B; Yield; Proteom;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Phee, B.K., Cho, J.H., P, S.Y., Jing, J.H., Lee, Y.H., Jeon, J. S., Bhoo, S.H., and Han, T.R. (2004) Proteomic analysis of the response of Arabidopsis chloroplast proteins to high light stress. Proteomics 4:3560-3568   DOI   ScienceOn
2 Cheng, S.H., Willmann, M.R., and Chen, H.C. (2002) Calcium signaling through protein kinase. The Arabidopsis calcium-dependent protein kinase gene family. Plant physiology. 129:469-485   DOI   ScienceOn
3 Fritsche, O. and Junge, W. (1996) Chloroplast ATP synthase, the clutch between proton flow and ATP synthesis is at the interface of subunit ${\delta}CF1$. Biochemica et Biophysica Acta. 1274 : 94-100   DOI   ScienceOn
4 Nelson, D.L and Cox, M.M. (2002) Lehninger Principles of Biochemistry 3판. 서울 외국서적, p1336. 728-729
5 Grossman, A.R., Bhaya, D., Apt, K.E., and Kehoe, D.M. (1995) Light-harvesting complexes in oxygenic photosynthesis : diversity, control, and evolution. Annu. Rev. Genet. 29 : pp. 231-288   DOI   ScienceOn
6 Lao, K. and Glazer, A. N. (1996) Proc. Natl.Acad. Sci. U.S.A 93:5258-5263
7 Paul, L., Angelis, De., Yang, N., and Weigel, P. H. (1995) Molecular cloning of the gene encoding ReeF, a DNA repair enzyme, from Streptococous pyogrnes Gene. 156:89-91   DOI   ScienceOn
8 Marinone, F., and Mureli, Carla. (1999) Galactinol in the leaves of the resurrection plant Boea hygroscopia. Phytochemistry. 51:499-505   DOI   ScienceOn
9 Dey, P.M. and Dixon, R.A. (Eels). (1985) Biochemistry of storage carbohydrates D-Galactose containing oligosaccharides in green plants. Academic press, New York. 99:53-130
10 Hook, W.A. and Harding, J.J. (1997) Molecular chaperone protect catalase against thermal stress. European Journal of Biochemistry. 247:380-385   DOI   ScienceOn
11 Saravitz, D.M., Pharr, D.M., and Carter, T.E. (1987) Galactinol synthase activity and soluble sugars in developing seeds of four soybean genotypes. Plant Physiology. 83:185-189   DOI   ScienceOn
12 Charles, A.K. and David, D.A. (2001) Correlated evolution of chloroplast heat shock protein expression in closely related plant species. American journal of Botany. 88:441-418
13 Heckathorn, S. A., Sharkey, P.S., and Coleman, J.S. (1998) The small methinoine-rich chloroplast heat shock protein protects photosystem II elcetron transport during heat stress. Plant physiology. 116: 439-444   DOI
14 Scheel, D. and Wastemack, C. (2002) Plant Signal Transduction. Oxford University Press. 324p., p97
15 UNEP. (2002) Executive summary. Final of UNEP / WMO Scientific assessment of ozone depletion : 2002. Prepared by the scientific assessment panel of the montraol protocol on substances that deplete the ozone layer. UNEP. Nairobi (Released 23 August 2002)
16 Kakani, V. G., Rebby, K.R., Zhao, D., and Sailaja, K. (2003) Field crop response to Ultraviolet-B radiation : a review. Agricultural and Forest Meteorology. 120:191-218   DOI   ScienceOn
17 Allen, D.J., Nogues, S., and Baker, N.R. (1998) Ozone depletion and increased UV-B radiation is there a real threat to photosynthesis? J. Exp. Bot 49, 1775-1788   DOI   ScienceOn
18 Santos, I., Fialgo, F., Almeida, J.A., and Salema, R. (2004) Biochemical and ultrastructual change in leaves of potato plants grown under supplementary UV-B radiation. Plant Science. 167:925-935   DOI   ScienceOn
19 Oh, S.N., Im, H. S., Oh, E. H., Lee, J.H., Kim, J.Y., Mun, J.H., Kim, Y.H., Lee, E.S., Kim, J., and Sul, D.G. (2004) Effects of benzo(a)pyrene of protein expression in Jurkat 'l-cells, Proteomics 4:3514-3526   DOI   ScienceOn
20 Harmon, A.C., Gribskov, M., Gubrium, E., and Harper, J.F. (2001) The CDPK superfamily of protein kinase. New Phytology. 151:175-183   DOI   ScienceOn
21 김순철.(2003) 들깨. 농촌진흥청. 300p., p35
22 Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254   DOI   ScienceOn
23 김동수, 박원만,(2004) Proteomic analysis Protocol. Genomine
24 Zhao, D., Reddy, K.R., kakani, V.G., Read, J., and Sullivan, J. (2003) Growth and physiological response of cotton (Gossypium hirsutum L.) to elevated carbon dioxide and Ultraviolet-B radiation under controlled environment conditions. Plant cell environment, pp 771-782
25 Savitch, L.V., Pocock, T., Krol, M., Wilson. K.E., Greenberg, B.M., and Huner, N.P.A. (2001) Effect of growth under UV-A radiation on $CO_2$ assimilation, Carbon partitioning, PSII photochemistry and resistance to UV-B radiation in Brassica napus cv, Topas. Aust J Plant Physiology. 28, 203-212
26 Ross, JR., Nam, K.H., D'Auria, J.C., and Pichersky, E. (1999) S-adenosyl-L-methionine : salicylic acid carboxyl methyltransferase, an enzyme involved in floral scent production and plant defense, represents a new class of plant methyltransferase. Arch Biochemical Biophysica 367:9-16   DOI   ScienceOn