• Journal of the Korea Institute of Military Science and Technology
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• v.12 no.2
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• pp.191-198
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• 2009

Some of the wargame simulators currently used in the Korean Army were developed by other countries, and do not adequately reflect the Korean Peninsula terrain and weapon systems. This implies that these war game simulators need to be calibrated with respect to the input parameters for properly assessing the effectiveness of the Korean weapon systems. In this paper, AWAM, a wargame simulator, is calibrated in terms of the time-based fighting power(FP). The FP data obtained from the Korea Combat Training Center(KCTC) are used as a reference, and the differences between the AWAM and KCTC FP data are calculated at certain points in time. Then, the Taguchi robust design method is adopted using the probabilities of hitting for the K-2 rifle as controllable input parameters. Two performance characteristics are used. One is the difference between the AWAM and KCTC FP data and the other is the score derived by grouping the difference data. For each case, optimal settings of the probabilities of hitting are determined such that the mean of each characteristic is close to 0 with its dispersion being as small as possible.

• Journal of the Korean Society for Precision Engineering
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• v.30 no.11
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• pp.1179-1185
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• 2013

In this study, the design optimization of the automotive side member is performed to maximize the crash energy absorption efficiency per unit weight. Design parameters which seriously influence on the frontal crash performance are selected through the sensitivity analysis using the Plackett-Burman design method. And also the design variables, which are determined from the sensitivity analysis, are optimized by two methods. One is conventional approximate optimization method which uses the statistical design of experiments (DOE) and response surface method (RSM). The other is a methodology derived from previous work by the authors, which is called sequential design of experiments (SDOE), to reduce a trial and error procedure and to find an appropriate condition for using micro-genetic algorithm. The proposed optimization technique shows that the automotive side member structure can be designed considering the frontal crash performance.

• Journal of Microbiology and Biotechnology
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• v.18 no.12
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• pp.1903-1907
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• 2008

Prodigiosin is a natural red pigment with algicidal activity against Cochlodinium polykrikoides, a major harmful red-tide microalga. To increase the yield of prodigiosin production by Hahella chejuensis KCTC 2396, significant medium components were determined using a two-level Plackett-Burman statistical design technique. Among 12 components included in basal medium, $NaHCO_3$, ${Na}_{2}{SiO}_{3}$, ${NH_4}{NO_3}$, ${Na}_{2}{SO}_{4}$ and $CaCl_2$ were determined to be important for prodigiosin production. The medium formulation was finally optimized using a Box-Behnken design as follows: 1% sucrose; 0.4% peptone; 0.1 % yeast extract; and (g/l): NaCl, 20.0; ${Na}_{2}{SO}_{4}$, 9.0; $CaCl_2$, 1.71; KCl, 0.4; and (mg/l): ${H_3}{BO_3}$, 10.0; KBr, 50.0; NaF, 2.0; $NaHCO_3$, 45.0; ${Na}_{2}{SiO}_{3}$, 4.5; ${NH_4}{NO_3}$, 4.5. The predicted maximum yield of prodigiosin in the optimized medium was 1.198 g/l by the Box-Behnken design, whereas the practical production was 1.495 g/l, which was three times higher than the basal medium (0.492 g/l).

• Journal of Microbiology and Biotechnology
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• v.19 no.11
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• pp.1385-1392
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• 2009

This work evaluates the effect of different amino acids on production of Cyclosporin (CyA) production in solid-state fermentation that was previously optimized for different fermentation parameters by one factor at-a-time for the maximum production of CyA by Tolypocladium inflatum MTCC557. Based on the Plackett-Burman design, glycerol, ammonium sulfate, $FeCl_3$, and inoculum size were selected for further optimization by response surface methodology (RSM). After identifying effective nutrients, RSM was used to develop mathematical model equations, study responses, and establish the optimum concentrations of the key nutrients for higher CyA production. It was observed that supplementation of medium containing (% w/w) glycerol, 1.53; ammonium sulfate, 0.95; $FeCl_3$, 0.18; and inoculum size 6.4 ml/5g yielded a maximum of 7,106 mg/kg as compared with 6,480 mg CyA/kg substrate using one factor at-a-time. In the second step, the effect of amino acids on the production of CyA was studied. Addition of $_L$-valine and $_L$-leucine in combination after 20 h of fermentation resulted in maximum production of 8,166 mg/kg.

• Transactions of the Korean Society of Automotive Engineers
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• v.16 no.3
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• pp.166-171
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• 2008

In this paper, the optimization design module for the brake system of a vehicle is developed. As using this module, design variables, that minimize an object function and satisfy nonlinear constraint conditions, can be found easily. Before an optimization is operated, Plackett-Burman design, one of the factorial design methods, is used to choose the design variables which affect a response function significantly. Using the response surface analysis, second order recursive model function, which informs a relation between design variables and response function, is estimated. In order to verify the reliability of the model function, analysis of variances(ANOVA) table is used. The value of design variables which minimize the model function and satisfy the constraint conditions is predicted through Sequential Quadratic-Programming (SQP) method. As applying the above procedure to a real vehicle simulation model and comparing the values of object functions of a current and optimized system, the optimization results are verified.

• Communications for Statistical Applications and Methods
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• v.14 no.3
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• pp.483-495
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• 2007

In a general fractional factorial design, the n-levels of a factor are coded by the $n^{th}$ roots of the unity. Pistone and Rogantin (2007) gave a full generalization to mixed-level designs of the theory of the polynomial indicator function using this device. This article discusses the optimal blocking scheme for nonregular designs. According to hierarchical principle, the minimum aberration (MA) has been used as an important criterion for selecting blocked regular fractional factorial designs. MA criterion is mainly based on the defining contrast groups, which only exist for regular designs but not for nonregular designs. Recently, Cheng et al. (2004) adapted the generalized (G)-MA criterion discussed by Tang and Deng (1999) in studying $2^p$ optimal blocking scheme for nonregular factorial designs. The approach is based on the method of replacement by assigning $2^p$ blocks the distinct level combinations in the column with different blocks. However, when blocking level is not a power of two, we have no clue yet in any sense. As an example, suppose we experiment during 3 days for 12-run Plackett-Burman design. How can we arrange the 12-runs into the three blocks? To solve the problem, we apply G-MA criterion to nonregular mixed-level blocked scheme via the mixed-level indicator function and give an answer for the question.

• Journal of Life Science
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• v.30 no.6
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• pp.522-531
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• 2020

The aim of this study was to establish the optimal medium composition for enhancing L(+)-lactic acid (LLA) production using response surface methodology (RSM). Lactobacillus paracasei SRCM201474 was selected as the LLA producer by productivity analysis from nine candidates isolated from kimchi and identified by 16S rRNA gene sequencing. Plackett-Burman design was used to assess the effect of eleven media components on LLA production, including carbon (glucose, sucrose, molasses), nitrogen (yeast extract, peptone, tryptone, beef extract), and mineral (NaCl, K₂HPO₄, MgSO₄, MnSO₄) materials. Glucose, sucrose, molasses, and peptone were subsequently chosen as promising media for further optimization studies, and a hybrid design experiment was used to establish their optimal concentrations as glucose 15.48 g/l, sucrose 16.73 g/l, molasses 39.09 g/l, and peptone 34.91 g/l. The coefficient of determination of the equation derived from RSM regression for LLA production was mathematically reliable at 0.9969. At optimum parameters, 33.38 g/l of maximum LLA increased by 193% when compared with MRS broth as unoptimized medium (17.66 g/l). Our statistical model was confirmed by subsequent validation experiments. Increasing the performance of LLA-producing microorganisms and establishing an effective LLA fermentation process can be of particular benefit for bioplastic technologies and industrial applications.

• Journal of Microbiology and Biotechnology
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• v.17 no.12
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• pp.1930-1936
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• 2007

A sequential optimization strategy based on statistical experimental designs was employed to enhance the production of cyclosporin A (CyA) by Tolypocladium inflatum DSMZ 915 in a submerged culture. A 2-level Plackett-Burman design was used to screen the bioprocess parameters significantly influencing CyA production. Among the 11 variables tested, sucrose, ammonium sulfate, and soluble starch were selected, owing to their significant positive effect on CyA production. A response surface methodology (RSM) involving a 3-level Box-Behnken design was adopted to acquire the best process conditions. Thus, a polynomial model was created to correlate the relationship between the three variables and the CyA yield, and the optimal combination of the major media constituents for cyclosporin A production, evaluated using the nonlinear optimization algorithm of EXCEL-Solver, was as follows (g/l): sucrose, 20; starch, 20; and ammonium sulfate, 10. The predicted optimum CyA yield was 113 mg/l, which was 2-fold the amount obtained with the basal medium. Experimental verification of the predicted model resulted in a CyA yield of 110 mg/l, representing 97% of the theoretically calculated yield.

• Ocean and Polar Research
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• v.39 no.4
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• pp.269-277
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• 2017

Medium compositions for the hyperthermophilic archaeon, Thermococcus onnurineus NA1 was statistically optimized to enhance formate-driven hydrogen ($H_2$) production by using response surface methodology. From the Plackett-Burman design-based experiment, it was confirmed that among the minor components of medium such as KCl, $MgSO_4$, $NH_4Cl$, Cystein-HCl, trace elements, Fe-EDTA and $CaCl_2$, the trace elements were screened as the only positively effective components with respect to $H_2$ production. Subsequently, the optimal concentrations of the trace elements and the major components of a medium such as NaCl, yeast extract and sodium formate were determined from the five-level central composite design (CCD)-based experiment. The resulting quadratic model predicted the maximum $H_2$ production of 46.6 mmol/L in serum bottle and it was validated experimentally using the optimal medium initially supplemented with 26.70 g/L of NaCl, 9.81 g/L of sodium formate, 3.50 g/L of yeast extract and 4.59 mL/L of trace elements. From the duplicate batch cultivations in the fermentor using the optimized medium, the a maximum $H_2$ production rate up to 71.8 mmol/L/h could be obtained, which was a 65% enhanced value compared with that obtained using the control medium, showing the high efficiency of the optimized medium.

• Journal of Microbiology and Biotechnology
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• v.30 no.8
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• pp.1252-1260
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• 2020

(R)-2-(4-hydroxyphenoxy)propionic acid (HPOPA) is a key intermediate for the preparation of aryloxyphenoxypropionic acid herbicides (R-isomer). In order to improve the HPOPA production from the substrate (R)-2-phenoxypropionic acid (POPA) with Beauveria bassiana CCN-A7, static cultivation and H₂O₂ addition were attempted and found to be conducive to the task at hand. This is the first report on HPOPA production under static cultivation and reactive oxygen species (ROS) induction. On this premise, the cultivation conditions and fermentation medium compositions were optimized. As a result, the optimal carbon source, organic nitrogen source, and inorganic nitrogen source were determined to be glucose, peptone, and ammonium sulfate, respectively. The optimal inoculum size and fermentation temperature were 13.3% and 28℃, respectively. The significant factors including glucose, peptone, and H₂O₂, identified based on Plackett-Burman design, were further optimized through Central Composite Design (CCD). The optimal concentrations were as follows: glucose 38.81 g/l, peptone 7.28 g/l, and H₂O₂ 1.08 g/l/100 ml. Under the optimized conditions, HPOPA titer was improved from 9.60 g/l to 19.53 g/l, representing an increase of 2.03-fold. The results obtained in this work will provide novel strategies for improving the biosynthesis of hydroxy aromatics.