• Title/Summary/Keyword: Picosecond

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Picosecond Fluorescence Lifetimes of Hematoporphyrin Derivatives in Solutions and in vitro (용액 및 시험관 실험에서의 헤마토포르피린 유도체 분자의 피코초 형광수명시간 분석)

  • Kim, Hyun-Soo;Chu, Sung-Sil;Kim, Gwi-Eon;Lee, Won-Young;Kim, Ung
    • Progress in Medical Physics
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    • v.6 no.2
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    • pp.61-70
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    • 1995

  • The picosecond time resolved fluorescence spectra of Hematoporphyrin Derivative (HPD) in both solutions and cancer cell are measured by a time correlated single photon counting system with a synchronously mode locked dye laser. Two exoponential decay components in the fluorescence spectra were observed. The slow decay(6.3 ㎱)and the fast one(350 ㎰)are attributed to be originated from monomers and dimers, respectively. The absorption and fluorescence measurements in steady state also showed the presence of a monomeric and dimeric forms of HPD molecules. The monomer lifetime in the cancer cell was measured to be longer than that in solution, which was expected from the blue shift and narrowing of the absorption spectra for HPD-treated in vitro. The relative amplitude of the fast component was found to be enhanced in cancer cell, strongly indicating the higher affinity of the dimer for the cancer cell.

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