Progress in Medical Physics (한국의학물리학회지:의학물리)
- Volume 6 Issue 2
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- Pages.61-70
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- 1995
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- 2508-4445(pISSN)
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- 2508-4453(eISSN)
Picosecond Fluorescence Lifetimes of Hematoporphyrin Derivatives in Solutions and in vitro
용액 및 시험관 실험에서의 헤마토포르피린 유도체 분자의 피코초 형광수명시간 분석
- Kim, Hyun-Soo (Department of Radiation Oncology, Yonsei University, College of Medicine) ;
- Chu, Sung-Sil (Department of Radiation Oncology, Yonsei University, College of Medicine) ;
- Kim, Gwi-Eon (Department of Radiation Oncology, Yonsei University, College of Medicine) ;
- Lee, Won-Young (Department of Microbiology, Yonsei University) ;
- Kim, Ung (Department of Physics, Yonsei University,)
- Published : 1995.12.01
Abstract
The picosecond time resolved fluorescence spectra of Hematoporphyrin Derivative (HPD) in both solutions and cancer cell are measured by a time correlated single photon counting system with a synchronously mode locked dye laser. Two exoponential decay components in the fluorescence spectra were observed. The slow decay(6.3 ㎱)and the fast one(350 ㎰)are attributed to be originated from monomers and dimers, respectively. The absorption and fluorescence measurements in steady state also showed the presence of a monomeric and dimeric forms of HPD molecules. The monomer lifetime in the cancer cell was measured to be longer than that in solution, which was expected from the blue shift and narrowing of the absorption spectra for HPD-treated in vitro. The relative amplitude of the fast component was found to be enhanced in cancer cell, strongly indicating the higher affinity of the dimer for the cancer cell.
헤마토포르피린 유도체는 광역학 치료를 위한 활성감응제로 이용되고 있다. 본 연구는 헤마토포르피린 유도체 분자의 암세포와의 에너지 전달과정을 조사하기 위하여 동시 모드록킹된 색소 레이저와 ~70 피코초 (