• Title/Summary/Keyword: Phytotoxicity

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Phytotoxic Effect of Herbicides on Upland Crops and Weeds (밭작물(作物) 및 잡초(雜草)에 대한 제초제(除草劑)의 약해(藥害) 약효(藥效))

  • Ryang, H.S.;Chun, J.C.;Yim, J.H.
    • Korean Journal of Weed Science
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    • v.4 no.1
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    • pp.69-78
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    • 1984
  • This study was conducted to select herbicides effective for upland crops and to investigate the cause of crop injury in peanut cultivated with mulching. Crop such as radish (Raphanus acanthiformis Moor.), Chinese cabbage (Brassica raps L.), soybean (Glycine max Merr.), Peanut (Archis hypogaea L.), and marsh mallow (Malva olitoria Nakai) were tolerant to napropamide [2-(${\alpha}$-naphthoxy)-N, N-diethylpropionamide], alachlor [2-chloro-2', 6'-diethyl-N-(methoxymethyl) acetanilide], trifluralin (${\alpha},{\alpha},{\alpha}$-trifluoro-2, 6-dinitro-N, N-dipropylp-toluidine) and nitrofen (2,4-dichlorophenyl-p-nitrophenylether). Napropamide, diphenamide (N, N-dimethyl-2, 2-diphenylacetamide) and alachlor were safe for red pepper (Capsicum annuum L.), eggplant (Solanum melongena L. and tomato (Lycopersicon esculentum Mill.), while trifluralin, nitrofen and chlonitrofen (2,4,6-trichlorophenyl-4-nitrophenyl ether) could be used for water melon (Citrullus battich Forsk.), carrot (Daucus carota L.) and lettuce (Lactuca scariola L.) without crop injury. Out of nine major weed species studied, Capsella bursa-pastoris Medicus was the most resistant species to the herbicides tested. Napropamide and alachlor could not control P. hydropiper, while P. oleracea and C. album were tolerant to diphenamide :and alachlor, respectively. Urea herbicides such as methabenzthiazuron [3-(2-benzothiazolyl)-1,3-dimethylurea], linuron [3-(3, 4-dichlorophenyl~l-methoxy-i-methyl urea], and isoproturon [3-(4-isopropylphenyl) -1, 1-dimethylurea]gave a great injury to the crops studied. The weeding effect was greater for broadleaf weeds than for grasses. Isoproturon and linuron provided good selectivity for marsh mallow and carrot, respectively. In peanut, the crop injury caused by Four herbicides studied was greater when cultivated with mulching than when cultivated without mulching. With dinitroaniline herbicides the crop injury decreased as the gaseous herbicide was removed out of mulching. Alachlor gave little phytotoxicity to peanut grown under mulching condition and nitralin [4-(methylsuphonyl)-2, 6-dinitro-N, N-dipropylaniline] showed less toxicity to the peanut than pendimenthalin (3,4-dimethyl-2, 6-dinitro-N-1-ethyl propylaniline) and trifluralin.

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Phytotoxicity of Endophytic Fungi and Characterization of a Phytotoxin Isolated from Gliocladium catenulatum from Pinus densiflora (식물내생곰팡이의 제초활성 검정 및 소나무에서 분리한 Gliocladium catenulatum이 생산하는 제초활성 물질의 특성 규명)

  • Choi, Gyung-Ja;Park, Joong-Hyeop;Kim, Heung-Tae;Lee, Seon-Woo;Choi, Jung-Sup;Hong, Kyung-Sik;Cho, Kwang-Yun;Kim, Jin-Cheol
    • The Korean Journal of Mycology
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    • v.32 no.1
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    • pp.8-15
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    • 2004
  • This study was conducted to discover new phytotoxins which may be used as lead molecules for the development of new herbicides. A total of 187 endophytic fungi were isolated from 11 plant species, which were collected from 8 locations in Korea. Their herbicidal activities were screened in vivo by herbicidal and duckweed bioassays after they were cultured in potato dextrose broth and rice solid media. Both fermentation broth and solid culture extract of Gliocladium catenulatum F0006 isolated from red pine (Pinus densiflora) showed 70% herbicidal activity only against cocklebur (Xanthium strumarium) out of the 10 weeds tested. Solid culture extract of F0034 isolated from arrowroot (Pueraria thunbergiana) exhibited 20 to 100% herbicidal activities against all of the weeds. Especially, shattercane (Sorghum bicolor), barnyardgrass (Echinochloa crus-galli), large crabgrass (Digitaria sanguinalis), and fall pauicum (Panicum dichtomiflorum) were sensitive to the solid culture extract of F0034. In addition, solid culture extract of F0043 isolated from red pine displayed 20% to 70% herbicidal activities only against 5 grass species, but not against 5 broad-leaf plant species. On the other hand, as the results of duckweed assay, 8 fermentation broths showed 100% growth inhibitory activity at concentrations less than 5.0% of culture supernatants and 12 solid cultures had a potent inhibitory activity against duckweed growth. A toxic metabolite was purified from the solid cultures of G. catenulatum F0006 by repeated column chromatography and bioassay. It caused a phytotoxic syndrome only on cocklebur out of the 10 weeds tested; it completely killed cocklebur seedlings at $500\;{\mu}g/ml$ and showed 85% herbicidal activity against cocklebur at $100\;{\mu}g/ml$. The molecular weight of the toxic metabolite is 238 daltons and its structure determination is underway.

Effects of Fermented Soybean Extracts and Rain-shelter System on Growth and Disease Occurrence of 'Niitaka' Pear (발효 콩 추출물과 비가림 시설이 '신고' 배나무의 생육과 병해충 발생에 미치는 영향)

  • Lim, Kyeong-Ho;Kim, Byeong-Sam;Kim, Deok-Hyeon;Son, Jang-Hwan;Park, Shin;Cho, Dong-Ho;Jung, Seok-Kyu;Choi, Hyun-Sug
    • Korean Journal of Organic Agriculture
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    • v.24 no.3
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    • pp.557-570
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    • 2016
  • Growth of 6-year old 'Niitaka' pear (Pyrus pyrifolia Nakai) trees and control of insect and disease occurrences were compared between fermented soybean extracts and rain-shelter system for two years. Foliar application of fermented soybean extracts was applied at 6 times as a pre-experiment in the open-field in 2013, with a rain-shelter system in 2014. Fermented soybean extract treatment increased foliar concentrations of approximately 0.46% T-N, 0.17% K, 0.19% Ca, and 0.06% Mg in 2013 compared to the control, with similar macro-nutrients between the control and soybean extract treatment observed in 2014. Rain-shelter system increased foliar concentrations of T-N, Ca, and Mg compared to the open-field. There were no significantly different between the control and soybean extract treatment for number of leaves per fruit, leaf dry weight, phytotoxicity, and completed shoot growth on August during the two years. Rain-shelter system increased leaf dry weight and did not affect phytotoxicity in the leaves. Fruit quality parameters were mostly similar to control and soybean extract treatment for two years, with higher fruit firmness observed for soybean extract treatment. Rain-shelter system advanced 4 days of harvest dates, and increased approximately 7.0 ton fruit yield per ha, 20 g mean fruit weight, and fruit soluble solid contents compared to open-field in 2014. Soybean extract treatment little suppressed occurrence of disease and insect on the leaves and fruits in both years. Rain-shelter system increased occurrence of Venturia nashicola on the leaves and to 63.8% of Gymnosporangium asiaticum on the fruits in 2014. Strong winds and storms in May elevated relative humidity in the rain-shelter system and caused high infection of the disease occurrence, requiring for an additional green control method. Soybean extract treatment little affected tree growth and would have initiated for a long-term study to evaluate tree physiological characteristics. Rain-shelter system improved fruit productivity and advanced harvest dates, which could have been more effective facility at a Thanks Giving Day between middle and end of September.

Screening of effective control agents against bacterial soft rot on Chinese cabbage in alpine area (고랭지 배추 무름병 방제를 위한 우수약제 선발)

  • Chung, Eun-Kyoung;Zhang, Xuan-Zhe;Yeoung, Young-Rog;Kim, Byung-Sup
    • The Korean Journal of Pesticide Science
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    • v.7 no.1
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    • pp.32-37
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    • 2003
  • Bacterial soft rot by Erwinia carotovora subsp. carotovora is one of the diseases causing the biggest problem in Chinese cabbage. Chemical screening was conducted to select effective agents for controlling bacterial soft rot. Control effect of antibiotics, plant activator, and Biokeeper (avirulent Erwinia) to soft rot were tested by in vitro assay, nursery test, and field experiment. The in vitro assay was done by paper disc method and potato slice method. The nursery test was performed by using mineral oil inoculation method with consistent disease induction. The in vitro assay showed that streptomycin, oxolinic acid, bronopol, and copper hydroxide significantly suppressed the growth of pathogenic bacterium and the decomposition of potato slice. However, plant activators including acibenzolar-S-methyl did not show the suppressive effect on the growth of pathogenic bacterium and the decomposition of potato slice. When applied by the nursery test condition using mineral oil inoculation method with Chinese cabbage 'Kangruckyeurum', Biokeeper, oxolinic acid, antibiotics streptomycin, validamycin, and copper compound provided 83.5%, 95.2%, 91.2%, 57.5% and 79.9% in control efficacy, respectively. However, the control effect of acibenzolar-S-methyl showed to be low to cause phytotoxicity. Also acibenzolar-S-methyl showed a significant control effect in the field experiment with Chinese cabbage 'Sanchon' in 2000, but the field experiment with Chinese cabbage 'Kangruckyeurum' in 2001 revealed it had phytotoxicity to Chinese cabbage. Such a difference was considered to be caused by differences in phytotoxic reaction of Chinese cabbage cultivars to the chemical. Streptomycin+copper, copper hydroxide and Biokeeper showed 79.7%, 71.9% and 60.9% in control efficacy, respectively, in the field experiment with Chinese cabbage 'Sanchon' in 2002.

Control Effect of Phosphate Compounds on Phytophthora Blight and Anthracnose of Pepper (고추 역병과 탄저병에 대한 인산염 화합물의 방제 효과)

  • Park, Seojeong;Kim, Heung Tae
    • The Korean Journal of Pesticide Science
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    • v.21 no.1
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    • pp.26-32
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    • 2017
  • Antimicrobial activity of 6 phosphate compounds as $H_3PO_3$, $H_3PO_4$, $K_3PO_4$, $K_2HPO_4$, $KH_2PO_4$ and $NH_4H_2PO_4$ against Phytophthora capsici JHAW 1-2 and Colletotrichum acutatum JC24 was investigated in this study. Inhibitory effect on zoospore release, zoosporangia germination and zoospore germination was superior than mycelial growth. Among 6 compounds, $H_3PO_3$ and $H_3PO_4$ showed the best antimicrobial activity against P. capsici JHAW 1-2. Diseases controlling activity of the phosphate compounds tested on seedling and fruit of pepper against Phytophthora blight was also better than those against anthracnose. When $H_3PO_3$ was applied to the pepper seedlings at a concentration of $100{\mu}g\;mL^{-1}$, severe phytotoxicity was occurred. However, with applying $10{\mu}g\;mL^{-1}$ of $H_3PO_3$ showed 100% the disease control efficacy. In case of $100{\mu}g\;mL^{-1}$ $H_3PO_4$ application showed excellent antimicrobial activity against P. capsici JHAW 1-2, and 56.7% of the disease control efficacy with no phytotoxicity. To investigate the control efficacy against anthracnose, conidia suspension was inoculated with non-wound and wound inoculation method on pepper fruit. Among 6 compounds, only $100{\mu}g\;mL^{-1}$ of $H_3PO_3$ and $H_3PO_4$ had a activity of more than 70%, but the control activity on other treatments was minimal or unacceptable.

Effect of Perfluidone - Bifenox Mixture II. Effect of Perfluidone - Bifenox Mixture on Weed Control, Plant Growth and Yield in Transplanted Rice (Perfluidone과 Bifenox의 혼합효과(混合效果) 제2보(第2報) Perfluidone과 Bifenox의 혼합제(混合劑가) 제초효과(除草效果)와 수도(水稻)의 생육(生育)에 미치는 영향(影響))

  • Ryang, H.S.;Jang, I.S.;Ma, S.Y.
    • Korean Journal of Weed Science
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    • v.5 no.2
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    • pp.194-201
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    • 1985
  • Effect of perfluidone(2-methyl-4-phenylsulphonyltrifluoromethylsulphoanilide) and bifenox(2,4-dichlorophenyl-3-methoxycarbonyl-4-nitrophenylether) mixture on weed control and plant growth and yield of transplanted rice was determined. Perfluidone-bifenox mixture applied at 75-105 g a.i./10a controlled effectively perennial weeds such as Sagittaria pygmaea Miq., Potamogeton dtstinctus A. Benn., Cyperus serotinus Rottb, Eleocharis kuroguwai Ohwi, including most annual weeds, but did not control Sagittaria trifolia L.. There was no phytotoxicity caused by perfluidone-bifenox mixture when applied at 100-140 g a.i./10a. The mixture at 150-210 g a.i./10a caused crop injury, but did not affect the yield. Phytotoxicity due to the mixture decreased as the application time was delayed.

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Creeping Bentgrass(Agrostis palustris Huds.) Control in Kentucky Bluegrass(Poa pratensis L.) Fairways (켄터키 블루그래스 페어웨이에서 문제가 되는 크리핑 벤트그래스 방제)

  • Tae Hyun-Sook
    • Asian Journal of Turfgrass Science
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    • v.19 no.2
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    • pp.65-72
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    • 2005
  • Creeping bentgrass (Agrostis palustris Huds.) had been the problematic weed for Kentucky bluegrass (Poa pratensis L.) fairway since it shows light green color all year. Experiment was carried out to determine the best herbicides combination to control creeping bentgrass in Kentucky bluegrass. fairway without injury. To investigate the efficacy of herbicides, five post-emergence herbicides of asulam WG ($87.6\%$), imazaquin SL ($20\%$), fenoxaprop-P-ethyl EC ($7\%$), mecoprop SL ($50\%$), triclopyr-TEA SL ($30\%$) and one pre-emergence herbicide pendimethalin EC ($31.7\%$) treated on 21 Sept. and 10 Nov. 2003. Kentucky bluegrass visual quality evaluated 30 and 50 days after application for phytotoxic effects of the herbicides. As a result, asulam WG (0.2g/$m^{2}$) and imazaquin SL (0.3ml/$m^{2}$) showed approximately $90\%$ of control in creeping bentgrass, but visual quality of Kentucky bluegrass significantly decreased from 20 to 50DAT (day after treatment). However, creeping bentgrass was acceptably controlled(over $80\%$) by fenoxaprop-P-ethyl EC (0.4ml/$m^{2}$)+triclopyr-TEA SL(0.3 ml/$m^{2}$) applied twice on 21 Sept. and 1 Oct. 2003 without serious injury on Kentucky bluegrass. Therefore, it is suggested that an application of fenoxaprop-P-ethyl EC (0.4ml/ $m^{2}$)+triclopyr-TEA SL (0.3 ml/$m^{2}$) may be more effective to control creeping bentgrass in Kentucky bluegrass with the least phytotoxicity by herbicides.

Changes of Salt Concentration by the Height of Ground Water Table on Disused Saltpan for Golf Course Construction Site (골프코스를 조성할 폐염전 매립지의 지하수위에 따른 토양산도 및 전기전도도 변화)

  • Lee, Dong-Ik;Kim, Ki-Dong;Joo, Young-Kyoo
    • Asian Journal of Turfgrass Science
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    • v.23 no.1
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    • pp.143-150
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    • 2009
  • High salt concentration is one of the most important limit factor on plant growth at a disused saltpan for golf course construction site. The control of salt in soil is definitely required and the monitoring of salt concentration in soil and ground water also required to amend soil physiochemical properties. This research was carried out to monitor the pH and salt concentration changes by the height of ground water. By the physiochemical analysis test, the soil contains a high salt concentration and classified as a slight alkaline clay soil. The height of ground water table changed to 1.3m, 3.3m and 2.8m at dry season(mid-late June, 2005), monsoon season(early-mid July) and after monsoon(late July), respectively. Compare to the average ground level of 2.9m, the ground water was over flooded about OAm at monsoon season. The electrical conductivity(ECe) was measured above $4.0dS{\cdot}m^{-1}$ over all areas and however, some areas showed over $20dS{\cdot}m^{-1}$. During a monsoon season, ECe was lowered to $1.2{\sim}15.0dS{\cdot}m^{-1}$, compared with those of the dry season. Therefore, the interception of the capillary connection between planting layer and ground water which contains high salt concentration should be adapted when golf courses are constructed on disused saltpan. The phytotoxicity caused by salt damage may be controled by the interception of capillary fringe of salt flow to the topsoil profile at the upper layer of the ground water table.

Uptake of Butachlor by Rice Seedlings and Its Phytotoxic Action to the Physiological Activities (수도묘(水稻苗)의 Butachlor 흡수(吸收) 및 약해발생(藥害發生) 특성(特性)에 관한 생리적(生理的) 연구(硏究))

  • Chung, Bong-Jin;Kwon, Yong-Woong
    • Korean Journal of Weed Science
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    • v.1 no.1
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    • pp.57-68
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    • 1981
  • To clarify the mode of uptake of butachlor (2-chloro-2', 6'-diethyl-N-(butoxymethyl) acetanilide) by rice seedlings, its phytotoxic action to growth and physiological activities, studies were conducted with rice seedlings, at the 6th or 7th leaf-stage, which were treated with nutrient solution containing butachlor 0, 1.8, 3.6, 7.2, 10.8 or 14.4 ppm for 1, 2 or 4 days, in other case, the solutions were thereafter renewed with the untreated nutrient solution for further growth. Uptake of butachlor by rice seedlings increased linearly with increase of its concentration and duration of uptake. Butachlor inhibited root growth more than shoot growth, furthermore, the inhibitory effect on the shoot growth was greater in height than in weight or leafing rate. After 4 day-treatment, the rates of shoot growth in weight were delayed for 4 days. Butachlor inhibited water uptake rapidly and linearly with increase of its external concentration. The reduced uptake of water was followed by slow increase in the stomatal resistance of leaves. Upon completion of butachlor treatment, rate of water uptake was recovered rapidly, but the stomatal resistance with lag in time. Butachlor did not affect the uptake of cation such as ammonium, potassium and calcium, but inhibited substantially uptake of nitrate in proportion to its concentration. Especially, butachlor did not affect synthesis and degradation of nitrate reductase. In addition, butachlor has shown much greater binding to the lipidic substances from rice roots than the proteinous material. The primary mechanism of phytotoxic action of butachlor does not seem to be its effect on the protein synthesis, but great affinity to membranes. The inhibition of water uptake, and its subsequent closure of stomates is thought very important for reduced growth under mild phytotoxicity.

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Weed Infestation and Effective Weed Control in Direct - Seeded Rice (담수(湛水) · 건답직파(乾畓直播) 벼재배(栽培)에서 잡초(雜草)의 발생특성(發生特性) 및 효과적(效果的)인 잡초방제(雜草防除))

  • Kim, H.H.;Lee, S.G.;Lee, J.C.;Song, I.M.;Shin, C.W.;Moon, C.S.;Pyon, J.Y.
    • Korean Journal of Weed Science
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    • v.18 no.1
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    • pp.1-11
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    • 1998
  • This experiment was conducted to investigate weed infestation and to determine effective weed control methods in direct-seeded rice. Twenty two weed species occurred in dry- and water-seeded rice, which was mainly composed of annual weeds. Dominant weed species in dry-seeded rice were Cyperus difformis, Echinochloa crus-galli, Aneilema keisak and Digitaria sanguinalis in discending order. Dominant weed species in water-seeded lice were E. crus-galli, C. difformis, Monochoria vaginalis and Scirpus juncoides. E. crus-galli emerged at 7 days after sowing. In water-seeded rice, E. crus-galli emerged at 5 days after sowing, and M vaginalis, S. juncoides and C. difformis at 8~10 days after sowing. Mean days to emerge important weeds was 20 days in dry-seeded rice and 13 days in water-seeded rice. Leaf development of weeds was faster than that of rice in dry-seeded rice. In water-seeded rice, E eras-galli was more vigorous than rice, but leaf development of other weeds were slower than that of rice. Changes in number of weeds and dry weight oil weed species varied depending upon weed species in the direct-seeded rice. Dry weight of weeds were increased greatly from 30 days to 60 days after sowing in dry-seeded rice. Number of weeds tended to increase up to 40 days after sowing drastically, and then trend of the increase was dull thereafter. Dry weight and number of weeds increased up to 20~60 days after sowing in water-seeded rice. Most effective herbicide treatments was foliar application of cyhalofop/bentazon at 20 days after sowing followed by fenoxaprop/bentazon at 45 days after sowing in dry-seeded rice. All herbicide treatments except foliar applications were very effective to control weeds in water-seeded rice. Slight phytotoxicity was observed in foliar applied fenoxaprop/bentazon at 45 days after sowing in water-seeded rice, but it did not affect rice yield.

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