• Biomolecules & Therapeutics
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• v.4 no.4
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• pp.301-309
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• 1996

LBD-001, a recombinant human interferon ${\gamma}$ produced by genetically engineered yeast as a host system, was intravenously administered to pregnant female rats (Sprague-Dawley) from day 7 to 17 of gestation at dose levels of 0.35$\times$10$^{6}$ , 0.69$\times$10$^{6}$ , and 1.38$\times$10$^{6}$ I.U./kg/day. As the control groups, hydrocortisone sodium succinate (5 or 10 mg/kg/day) was also similarly administered. Teratological effects of the test agents on fetuses and development of offsprings (F1 rats) were investigated. (1) No significant changes by the treatment of LBD-001 were observed in body weight, food and water consumption, feeding and nursing behaviors, and autopsy of pregnant or lactating mother rats. However, in hydrocortisone sodium succinate (10 mg/kg/day)-treated group, significant decreases of body weight on day 16, 18, and 20 of gestation and food consumption on day 20 of gestation and outstanding atrophy of thymus and adrenals were observed in two rats autopsied on day 20 of gestation. (2) No significant changes in resorption rate, skeletal or visceral development of fetuses, and physical or sensory development of offsprings (Fl) by the treatment of LBD-001 were detected. In hydrocortisone sodium succinate (10 mg/kg/day)-treated group, however, there were significant decreases of body weight of fetuses, delay of ossification, temporary delay of body weights of offsprings (F1) on day 1 and 3 of lactation, and increased tendency of stillborn rate and malformation rate of bone. The results show that LBD-001 at the dose of 1.38$\times$10$^{6}$ I.U./kg/day or less is not teratogenic in organogenesis of fetuses and the development of offsprings (F1). Meanwhile, hydrocortisone sodium succinate (10 mg/kg/day) seems to delay ossification of fetuses and temporarily retard the development of offsprings (Fl).

• Journal of the Korean Society for Nondestructive Testing
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• v.30 no.4
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• pp.359-366
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• 2010

For the application of Lamb wave to structural health monitoring(SHM), understanding its physical characteristic and interaction between Lamb wave and defect of the host structure is an important issue. In this study, reflected, transmitted and mode converted Lamb waves at discontinuity of a plate structure were simulated and the amplitude ratios are calculated theoretically using Modal decomposition method. The predicted results were verified comparing with finite element method(FEM) and experimental results simulating attached PZTs. The result shows that the theoretical prediction is close to the FEM and the experimental verification. Moreover, quantitative estimation method was suggested using amplitude ratio of Lamb wave at discontinuity.

• Wind and Structures
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• v.31 no.3
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• pp.241-253
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• 2020

The inerter-based vibration absorber (IVA) is an enhanced variation of Tuned Mass Damper (TMD). The parametric optimization of absorbers in the previous research mainly considered only two decision variables, namely frequency ratio and damping ratio, and aimed to minimize peak displacement and acceleration individually under the excitation of the across-wind load. This paper extends these efforts by minimizing two conflicting objectives simultaneously, i.e., the extreme displacement and acceleration at the top floor, under the constraint of the physical mass. Six decision variables are optimized by adopting a constrained multi-objective evolutionary algorithm (CMOEA), i.e., NSGA-II, under fluctuating across- and along-wind loads, respectively. After obtaining a set of optimal individuals, a decision-making approach is employed to select one solution which corresponds to a Tuned Mass Damper Inerter/Tuned Inerter Damper (TMDI/TID). The optimization procedure is applied to parametric optimization of TMDI/TID installed in a 340-meter-high building under wind loads. The case study indicates that the optimally-designed TID outperforms TMDI and TMD in terms of wind-induced vibration mitigation under different wind directions, and the better results are obtained by the CMOEA than those optimized by other formulae. The optimal TID is proven to be robust against variations in the mass and damping of the host structure, and mitigation effects on acceleration responses are observed to be better than displacement control under different wind directions.

• Journal of KIISE
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• v.43 no.9
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• pp.947-952
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• 2016

Most data centers attempt to consolidate servers using virtualization technology to efficiently utilize limited physical resources. Moreover, virtualized systems have commonly adopted contents-based page sharing mechanism for page deduplication among virtual machines (VMs). However, previous page sharing schemes are limited by the inability to effectively manage accumulated hints which mean sharable pages in stack. In this paper, we propose a priority-based hint management scheme to efficiently manage accumulated hints, which are sent from guest to host for improving page sharing opportunity in virtualized systems. Experimental results show that our scheme removes pages with low sharing potential, as compared with the previous schemes, by efficiently managing the accumulated pages.

• Korean Journal of Plant Resources
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• v.22 no.5
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• pp.389-393
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• 2009

Regional distribution of mistletoe (Viscum album var.coloratum) and its habitual environments were investigated in order to obtain the basal data on the artificial propagation to cope with its increasing consume for medicine. Mistletoes inhabited throughout the overall region of the South Korea investigated. They were parasitic mainly to the Quercus spp. including Q. serrata and rarely to the Castanea crenata var. dulcis, Prunus serrulata var. spontanea, Alnus japonica, and Pyrus pyrifolia, etc. Mistletoes were not observed on the conifers such as Pinus densiflora and Pinus koraiensis and some deciduous broad-leaved trees species such as Zelkova serrata, Diospyros kaki, Acer mono, Acer palmatum, and Morus alba. Their habitats were located from zero to 1,200 m above sea level nevertheless the direction or slope of the mountains, suggesting that artificial propagation can be carried out nation widely to the well-grown parasite tree species. Parasitic specificity related to the physical and chemical characteristic of the epidermal tissues will be studied further.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.7
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• pp.1079-1083
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• 2015

Activating macrophage cells play an important role in the host immune defense system. In this paper, immuno-modulatory activities of polysaccharides separated from Jubak (JPS) in macrophage cells were investigated. Immuno-modulatory activities were estimated based on cell proliferation, nitric oxide (NO) and cytokine production, degree of mitogen-activated protein kinases (MAPKs), and nuclear factor (NF)-${\kappa}B$ phosphorylation in RAW264.7 macrophage cells. JPS (62.5 to $250{\mu}g/mL$) did not induce a cytotoxic event. Additionally, NO and proinflammatory cytokines (tumor necrosis factor-${\alpha}$ and interleukin-6) production significantly increased in a dose-dependent manner. Similarly, phosphorylation of MAPKs and NF-${\kappa}B$ increased upon JPS treatment. Therefore, our results suggest that polysaccharides separated from Jubak can induce macrophage activation through MAPK and NF-${\kappa}B$ signaling and induction of Th1 polarization.

• Korean journal of applied entomology
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• v.5_6
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• pp.59-63
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• 1968

This Paper deals With the biology and the life Cycle of Eriophyes kuko KISHIDA. 1. The host of Eriophyes kuko KISHIDA is Lycium chinense MILL (Boxthorn), Solanum nigrum LINNE(Morel) and Capacium annum LINNE(Red pepper) etc. 2. Eriophyes kuko KISHIDA is migrated and propagated by physical carriers. The direction of the wind has a great influence upon migration and dispersal. 3. The number of e99s deposited by a adult female is $28.5\pm0.449$ egg period is $7.0\pm0.447$ and larval period is $4.33\pm0.667$ days respectively. 4. The size of gall diameter on the leaf of Lycium Chinense MILL after boring in each date by Eriophyes kuke KISHIDA is as follows, 1st day after boring is $290.5\pm0.3415\mu$ End 25st day after boring is $3260.5\pm0.5157\mu$ in average. 5. Adult periods are from 25 to 30 days and the egg laying period is 6 days. 6. Eriophyes kuko KISHIDA has 6 to 7 generations in a year, and hibernate they over winter in the gall as an adult. The first adult appears in late May.

• Journal of Microbiology and Biotechnology
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• v.9 no.5
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• pp.631-636
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• 1999

The thermostable endo-chitosanase gene from the isolated strain Bacillus sp. KFB-C108 was identified on the basis of a phylogenetic analysis of the 16S rRNA gene sequence, and was cloned into plasmid pUCl8 using E. coli $DH5\alpha$ as the host strain. Positive clones carrying recombinant plasmids (pKCHO I and pKCHO II) containing chitosanase activity were selected using the direct activity staining method. Detailed physical maps showed the two plasmid inserts were identical except that the KCHO II insert (2.6 kb) was 1.8 kb smaller than that of the KCHO I. The recombinant plasmids were analyzed to determine the essential region for chitosanase activity, and a 1.3-kb fragment (KCHO-6) was subcloned into pTrc99A using the EcoRI and BamHI sites to construct pTrc99A/KCHO-6(pTrEB13). The resulting plasmid exerted high chitosanase activity upon transformation of E. coli $DH5{\alpha}cells$, overproducing about 20 times more in the cloned cells than in the wild-type cells. The cloned chitosanase protein exhibited the same molecular weight and catalytic activity similar to those of Bacillus sp. KFB-C108. The cloned enzyme was an endo-type that produced a chitosan tetramer as the major reaction product; however, it produced no monomers or dimers.

• Journal of Microbiology and Biotechnology
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• v.30 no.9
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• pp.1430-1435
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• 2020

Bacterial cellulose (BC) has outstanding physical and chemical properties, including high crystallinity, moisture retention, and tensile strength. Currently, the major producer of BC is Komagataeibacter xylinus. However, due to limited tools of expression, this host is difficult to engineer metabolically to improve BC productivity. In this study, a regulated expression system for K. xylinus with synthetic ribosome binding site (RBS) was developed and used to engineer a BC biosynthesis pathway. A synthetic RBS library was constructed using green fluorescent protein (GFP) as a reporter, and three synthetic RBSs (R4, R15, and R6) with different strengths were successfully isolated by fluorescence-activated cell sorting (FACS). Using synthetic RBS, we optimized the expression of three homologous genes responsible for BC production, pgm, galU, and ndp, and thereby greatly increased it under both static and shaking culture conditions. The final titer of BC under static and shaking conditions was 5.28 and 3.67 g/l, respectively. Our findings demonstrate that reinforced metabolic flux towards BC through quantitative gene expression represents a practical strategy for the improvement of BC productivity.

• Nuclear Medicine and Molecular Imaging
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• v.40 no.2
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• pp.82-89
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• 2006

Bone metastasis is a common sequelae of solid malignant tumors such as prostate, breast, lung, and renal cancers, which can lead to various complications, including fractures, hypercalcemia, and bone pain, as well as reduced performance status and quality of life it occurs as a result of a complex pathophysiologic process between host and tumor cells leading to cellular invasion, migration adhesion, and stimulation of osteoclastic and osteoblastic activity. Several sequelae occur as a result of osseous metastases and resulting bone pain can lead to significant debilitation. A multidisciplinary approach is usually required not only to address the etiology of the pain and its complicating factors but also to treat the patient appropriately. Pharmaceutical therapy of bone pain, includes non-steroidal analgesics, opiates, steroids, hormones, bisphosphonates, and chemotherapy. While external beam radiation therapy remains the mainstay of pain palliation of a solitary lesions, bone seeking radiopharmaceuticals have entered the therapeutic armamentarium for the treatment of multiple painful osseous lesions. $^{32}P,\;^{89}SrCl,\;^{153}Sm-EDTMP,\;^{188}Re/^{186}Re-HEDP,\;and\;^{177}Lu-EDTMP$ can be used to treat painful osseous metastases. These various radiopharmaceuticals have shown good efficacy in relieving bone pain secondary to bone metastasis. This systemic form of metabolic radiotherapy is simple to administer and complements other treatment options. This has been associated with improved mobility in many patients, reduced dependence on narcotic and non-narcotic analgesics, improved performance status and quality of life, and, in some studios, improved survival. All of these agents, although comprising different physical and chemical characteristics, offer certain advantages in that they are simple to administer, are well tolerated by the patient if used appropriately, and can be used alone or in combination with the other forms of treatment. This article illustrates the salient features of these radiopharmaceuticals, including the usual therapuetic dose, method of administration, and indications for use and also describe about the pre-management checklists, and jndication/contraindication and follow-up protocol.