• Animal Systematics, Evolution and Diversity
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• 제17권1호
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• pp.35-47
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• 2001

한국산 가재 (Cambaroides similis)의 후기배발생과정을 상세히 기재하고, Astacidae와 미국산 가재과(Cambaridae)에서 후기배발생과정이 알려진 종들과 아시아산 가재속 (Cambaroides)의 종들에서, 그 계통적 유연관계를 비교 조사하였다. 한국산 가재의 포란한 암컷은 5월에 출현하였다. 제1, 2 어린시기는 15.0$\pm$0.5$^{\circ}C$에서 각기 2주일과 4주일이 소요되었다. 제3 어린시기에는 모체로부터 이탈하였다. 제1 어린시기의 제1소악과 제2 어린시기의 제2촉각, 제2소악, 복지, 그리고 미지에서 그 형태적 특징들이 가재과(Cambaridae)보다 Astacidae와 매우 유사함을 알 수 있었다.

• 생명과학회지
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• 제16권2호
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• pp.345-351
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• 2006

2004년부터 2005년까지 제주도에서 전형적인 연쇄구균증상을 나타내는 양식넙치로부터 Streptococcus sp.를 분리하였다. 생화학적 성상 조사와 multiplex PCR assay법을 통해 S. iniae 94 균주를 동정하였다. RAPD fingerprint profile을 조사해 본 결과 A, B, C-type 총 3가지의 genotype을 확인하였으며, 동일종에 대해 외국에서 보고된 선행 연구결과와 비교하였을 경우, 지리적으로 다른 유전적 다형현상을 나타내는 것으로 추정되었다. 근래에 제주도에 우점하는 genotype은 A-type이었고, RAPD fingerprint profile은 약 2,000 bp, 1,300 bp, 1,000 bp, 850 bp, 550 bp의 밴드 양상을 나타내었다. S. iniae의 serotype 동정에 유효한 것으로 보고되어진 P14 random primer는 유전적 다형현상에 의해 제주도에서 분리된 S. iniae의 serotype 동정에 적합하지 않은 것으로 판단되었다.

• Journal of Microbiology and Biotechnology
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• 제20권4호
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• pp.651-658
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• 2010

Garcinia is commonly found in Malaysia, but limited information is available regarding endophytic fungi associated with this plant. In this study, 24 endophytic fungi were successfully recovered from different parts of two Garcinia species. Characterization of endophytic fungi was performed based on the conserved internal transcribed spacer (ITS) region sequence analysis and the antimicrobial properties. Results revealed that fruits of the plant appeared to be the highest inhabitation site (38%) as compared with others. Glomerella sp., Guignardia sp., and Phomopsis sp. appeared to be the predominant endophytic fungi group in Garcinia mangostana and Garcinia parvifolia. Phylogenetic relationships of the isolated endophytic fungi were estimated from the sequences of the ITS region. On the other hand, antibacterial screening showed 11 of the isolates possessed positive response towards pathogenic and nonpathogenic bacteria. However, there was no direct association between certain antibacterial properties with the specific genus observed.

• 한국수산과학회지
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• 제34권2호
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• pp.137-144
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• 2001

Sequences of partial 18S rDNA have been analyzed to elucidate genetic diversity of scallops collected around Korean sea, The scallops used in genetic comparison are Argopecten irradians concentricus, Amusium japonicum japonicum, Chlamys farreri farreri, Chlamys (Swiftopecten) swifti and Patinopecten yessoensis. The 18S rDNA sequences were aligned by Clustalx program. Phylogenetic tree was drawn by Treecon program, The scallops were divided into two groups-the Family Pectinidae containing A. japonicum japonicum and the Family Propeamussiidae containing Argopecten, Chlamys and Patinopecten genera. The Family Propeamussiidae was also divided into the Supergenera Aequipecten containing A. irradians concentricus and Supergenera Chlamys containing C. farreri farreri, C. swifti and P. yessoensis. The species of C. swifti was closer to the P. yessoensis rather than C. farreri farreri in respect to nuclear 18S rDNA sequence.

• Asian-Australasian Journal of Animal Sciences
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• 제21권12호
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• pp.1696-1702
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• 2008

In order to learn the origin of the Chinese Mongolian horse, we analyzed polymorphisms within the mtDNA D-loop variable region in 305 horses of 6 types of 3 different breeds, including one imported breed, one cultivated breed and 4 types of one local breed. We detected 13 different haplotypes, and subsequent sequence analysis showed that all 6 horse types were genetically diverse. By constructing a cladogram of mtDNA D-loop sequences from the 6 horse types along with homologous sequences from several other horse types obtained from GenBank, we showed that Chinese Mongolian horses have a close genetic relationship with other horse types from Mongolia. We also speculate that several Chinese Mongolian horses descended from Przewalskii horse. Additionally, the 13 haplotypes were dispersed throughout the cladogram, suggesting that Chinese Mongolian horses likely originated from multiple female ancestors. A phylogenetic map of the 6 horse types showed that the genetic relationship between the local Wuzhumuqin and Wushen types were the closest. The Xinihe and Baerhu were also closely related to each other, and slightly more distantly related to the cultivated Sanhe breed. All five of the local Chinese horse types had a much more distant relationship with the imported Thoroughbred breed.

• Asian-Australasian Journal of Animal Sciences
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• 제25권7호
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• pp.935-944
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• 2012

Amur grayling, Thymallus grubii, is an important economic cold freshwater fish originally found in the Amur basin. Currently, suffering from loss of habitat and shrinking population size, T. grubii is restricted to the mountain river branches of the Amur basin. In order to assess the genetic diversity, population genetic structure and infer the evolutionary history within the species, we analysised the whole mitochondrial DNA control region (CR) of 95 individuals from 10 rivers in China, as well as 12 individuals from Ingoda/Onon and Bureya River throughout its distribution area. A total of 64 variable sites were observed and 45 haplotypes were identified excluding sites with gaps/missing data. Phylogenetic analysis was able to confidently predict two subclade topologies well supported by maximum-parsimony and Bayesian methods. However, basal branching patterns cannot be unambiguously estimated. Haplotypes from the mitochondrial clades displayed local homogeneity, implying a strong population structure within T. grubii. Analysis of molecular variance detected significant differences among the different geographical rivers, suggesting that T. grubii in each river should be managed and conserved separately.

• Journal of Microbiology and Biotechnology
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• 제24권12호
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• pp.1606-1608
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• 2014

During an investigation of the fungal diversity of Korean soils, four Penicillium strains could not be assigned to any described species. The strains formed monoverticillate conidiophores with occasionally a divaricate branch. The conidia were smooth or finely rough-walled, globose to broadly ellipsoidal and $2.5-3.5{\times}2.0-3.0{\mu}m$ size. Their taxonomic novelty was determined using partial ${\beta}$-tubulin gene sequences and the ribosomal internal transcribed spacer region. The phylogenetic analysis showed that the isolates belonged to section Lanata-Divaricata and were most closely related to Penicillium raperi. Phenotypically, the strains differed from P. raperi in having longer and thicker stipes and thicker phialides. Strain KACC $47721^T$ from bamboo field soil was designated as the type strain of the new species, and the species was named Penicillium koreense sp. nov., as it was isolated from various regions in Korea.

• Animal Systematics, Evolution and Diversity
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• 제32권1호
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• pp.1-8
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• 2016

Vermamoeba vermiformis is a very important free-living amoeba for human health in association with Legionnaires' disease and keratitis. This interesting amoeba was firstly isolated from a freshwater of Dokdo (island), which was historically used for drinking water. Trophozoites and cyst forms of V. vermiformis strain MG1 are very similar to previous reported species. Trophozoites of V. vermiformis strain MG1 showed cylindrical shape with prominent anterior hyaline region. The average ratio of length and width was about 6.5. Typically, cysts of the strain MG1 showed a spherical or slightly ovoidal shape with smooth wall, and lacked cyst pores. Some cysts had crenulate-walled ectocyst, which was separated from endocyst wall. Further, 18S rRNA gene sequence of V. vermiformis strain MG1 showed very high similarity to other V. vermiformis species (99.4%-99.9% identity). Molecular phylogenetic analysis based on 18S rRNA gene sequences clearly confirmed that the isolate was one strain of V. vermiformis with maximum bootstrap value (maximum likelihood: 100%) and Bayesian posterior probability of 1. Thus, the freshwater of Dokdo in Korea could harbor potentially pathogenic amoeba that may cause diseases in humans.

• Animal Systematics, Evolution and Diversity
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• 제27권2호
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• pp.159-163
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• 2011

DNA barcoding of Bryozoa or "moss animals" has hardly advanced and lacks reference sequences for correct species identification. To date only a small number of cytochrome c oxidase subunit I (COI) sequences from 82 bryozoan species have been deposited in the National Center for Biotechnology Information (NCBI) GenBank and Barcode of Life Data Systems (BOLD). We here report COI data from 53 individual samples of 29 bryozoan species collected from Korean seawater. To our knowledge this is the single largest gathering of COI barcode data of bryozoans to date. The average genetic divergence was estimated as 23.3% among species of the same genus, 25% among genera of the same family, and 1.7% at intraspecific level with a few rare exceptions having a large difference, indicating a possibility of presence of cryptic species. Our data show that COI is a very appropriate marker for species identification of bryozoans, but does not provide enough phylogenetic information at higher taxonomic ranks. Greater effort involving larger taxon sampling for the barcode analyses is needed for bryozoan taxonomy.

• Journal of Forest and Environmental Science
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• 제30권3호
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• pp.307-313
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• 2014

This study was conducted to distinguish the pines that are too similar to differentiate using conventional methods. Pinus densiflora and Pinus sylvestris have similar anatomical structure. They both have window-like pits and dentate ray tracheids, so it is not easy to distinguish the plants. We tried to find molecular markers by comparing chloroplast DNA sequences to differentiate the pines growing in Korea. We used P. densiflora, P. densiflora for. multicaulis, P. sylvestris, P. rigida, P. rigitaeda, P. koraiensis, and P. bungeana for this study. We found that the non-coding intergenic region of trnT(UGU) and trnL(UAA) genes have differences among the species. We designed a primer set to amplify the region efficiently and compared the PCR product sequences using CLC Workbench programs to find the polymorphism. We could distinguish the species using the sequences of the amplified region and the sequences were reproducible from the pines collected in Korea.