Browse > Article
http://dx.doi.org/10.4014/jmb.0909.09030

Molecular Diversity of Fungal Endophytes Isolated from Garcinia mangostana and Garcinia parvifolia  

Sim, Jiun-Horng (Department of Biomedical Science, Faculty of Medicine and Health Sciences, University Putra Malaysia)
Khoo, Chai-Hoon (Department of Biomedical Science, Faculty of Medicine and Health Sciences, University Putra Malaysia)
Lee, Learn-Han (Department of Biomedical Science, Faculty of Medicine and Health Sciences, University Putra Malaysia)
Cheah, Yoke-Kqueen (Department of Biomedical Science, Faculty of Medicine and Health Sciences, University Putra Malaysia)
Publication Information
Journal of Microbiology and Biotechnology / v.20, no.4, 2010 , pp. 651-658 More about this Journal
Abstract
Garcinia is commonly found in Malaysia, but limited information is available regarding endophytic fungi associated with this plant. In this study, 24 endophytic fungi were successfully recovered from different parts of two Garcinia species. Characterization of endophytic fungi was performed based on the conserved internal transcribed spacer (ITS) region sequence analysis and the antimicrobial properties. Results revealed that fruits of the plant appeared to be the highest inhabitation site (38%) as compared with others. Glomerella sp., Guignardia sp., and Phomopsis sp. appeared to be the predominant endophytic fungi group in Garcinia mangostana and Garcinia parvifolia. Phylogenetic relationships of the isolated endophytic fungi were estimated from the sequences of the ITS region. On the other hand, antibacterial screening showed 11 of the isolates possessed positive response towards pathogenic and nonpathogenic bacteria. However, there was no direct association between certain antibacterial properties with the specific genus observed.
Keywords
Fungal endophyte; Garcinia mangostana; Garcinia parvifolia; ITS rDNA; antibacterial;
Citations & Related Records

Times Cited By Web Of Science : 3  (Related Records In Web of Science)
연도 인용수 순위
  • Reference
1 Crozier, J., S. E. Thomas, M. C. Aime, H. C. Evans, and K. A. Holmes. 2006. Molecular characterization of fungal endophytic morphospecies isolated from stems and pods of Theobroma cacao. Plant Pathol. 55: 783-791.   DOI   ScienceOn
2 Enrico, G. A. L. and A. V. D. A. A. Hubertus. 1995. Taxonomy, sexuality and mating types of Diaporthe adunca. Mycol. Res. 99: 1409-1416.   DOI   ScienceOn
3 Kim, S., D.-S. Shin, T. Lee, and K.-B. Oh. 2004. Periconicins, two new fusicoccane diterpenes produced by an endophytic fungus Periconia sp. with antibacterial activity. J. Nat. Prod. 67: 448-450.   DOI   ScienceOn
4 Liang-Dong, G., H. Guo-Rui, and W. Yu. 2008. Seasonal and tissue age influences on endophytic fungi of Pinus tabulaeformis (Pinaceae) in the Dongling Mountains, Beijing. J. Integr. Plant Biol. 50: 997-1003.   DOI   ScienceOn
5 Nakatani, K., M. Atsumi, T. Arakawa, K. Oosawa, S. Shimura, N. Nakahata, and Y. Ohizumi. 2002. Inhibitions of histamine release and prostaglandin E2 synthesis by mangosteen, a Thai medicinal plant. Biol. Pharm. Bull. 25: 1137-1141.   DOI   ScienceOn
6 Qi, F. H., T. Z. Jing, Z. X. Wang, and Y. G. Zhan. 2009. Fungal endophyte from Acer ginnala Maxim: Isolation, identification and their yield of gallic acid. Lett. Appl. Microbiol. doi:10.1111/ j.1472-765X.2009.02626.x (In Press).   DOI   ScienceOn
7 Won, H. and S. S. Renner. 2005. The internal transcribed spacer of nuclear ribosomal DNA in the gymnosperm Gnetum. Mol. Phylogenet. Evol. 36: 581-597.   DOI   ScienceOn
8 Williamson, P. M., A. S. Highet, W. Gams, K. Sivasithamparam, and W. A. Cowling. 1994. Diaporthe toxica sp. nov., the cause of lupinosis in sheep. Mycol. Res. 98: 1364-1368.   DOI   ScienceOn
9 Tefera, T. and S. Vidal. 2009. Effect of inoculated method and plant growth medium on endophytic colonization of sorghum by the entomopathogenic fungus Beauveria bassiana. Biocontrol 54: 663-669.   DOI   ScienceOn
10 White, T. F., T. Bruns, S. Lee, and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, pp. 315-322. In M. A. Innis, D. H. Gelfand, F. S. Sninsky, and T. T. White (eds.). PCR Protocol: A Guide to Methods and Applications. Academic Press, San Diego.
11 Wilson, D. 1995. Endophytes - the evolution of a term and clarification of its use and definition. Oikos 73: 274-276.   DOI   ScienceOn
12 Zin, N. M., N. I. M. Sarmin, N. Ghadin, D. F. Basri, N. M. Sidik, W. M. Hess, and G. A. Strobel. 2007. Bioactive endophytic streptomycetes from the Malay Peninsula. FEMS Microbiol. Lett. 274: 83-88.   DOI   ScienceOn
13 Li, Y. S., J. Y. Liu, Y. M. Ma, and R. X. Tan. 2005. Anti-Helicobacter pylori substances from endophytic fungal cultures. World J. Microbiol. Biotechnol. 21: 553-558.   DOI   ScienceOn
14 Moongkarndi, P., N. Kosem, O. Luanratana, S. Jongsomboonkusol, and N. Pongpan. 2004. Antiproliferative activity of Thai medicinal plants extracts on human breast adenocarcinoma cell line. Fitoterapia 75: 375-377.   DOI   ScienceOn
15 Mungo, C. M., A. M. Emechebe, and D. A. Florini. 1998. Isolation of Sphaceloma sp. from four cowpea plant parts using eight media. Crop Prot. 17: 341-343.   DOI   ScienceOn
16 Petrini, O. and P. J. Fisher. 1988. A comparative study of fungal endophytes in xylem and whole stem of Pinus sylvestris and Fagus sylvatica. Trans. Br. Mycol. Soc. 91: 233-238.   DOI
17 Phongpaichit, S., J. Nikom, N. Rungjindamai, J. Sakayaroj, N. Hutadilok-Towatana, V. Rukachaisirikul, and K. Kirtikara. 2007. Biological activities of extract from endophytic fungi isolated from Garcinia plants. FEMS Immunol. Med. Microbiol. 56: 517-525.
18 Phongpaichit, S., N. Rungjindamai, V. Rukachaisirikul, and J. Sakayaroj. 2006. Antimicrobial activity in cultures of endophytic fungi isolated from Garcinia species. FEMS Immunol. Med. Microbiol. 48: 367-372.   DOI   ScienceOn
19 Stinson, M., D. Ezra, W. M. Hess, J. Sears, and G. Strobel. 2003. An endophytic Gliocladium sp. of Eucryphia cordifolia producing selective volatile antimicrobial compound. Plant Sci. 165: 913-922.   DOI   ScienceOn
20 Strobel, G. A. 2003. Endophytes as sources of bioactive products. Microbes Infect. 5: 535-544.   DOI   ScienceOn
21 Atmosukarto, I., U. Castillo, W. M. Hess, J. Sear, and G. Strobel. 2005. Isolation and characterization of Muscodor albus I-41.3s, a volatile antibiotic producing fungus. Plant Sci. 169: 854-861.   DOI   ScienceOn
22 Brayford, D. 1990. Variation in Phomopsis isolates from Ulmus species in the British Isles and Italy. Mycol. Res. 94: 691-697.   DOI
23 Eichner, C. A., R. W. Erb, K. N. Timmis, and I. Wagner-Dobler. 1999. Thermal gradient gel electrophoresis analysis of bioprotection from pollutant shocks in the activated sludge microbial community. Appl. Environ. Microbiol. 65: 102-109.
24 Ezra, D., W. M. Hess, and G. A. Strobel. 2004. New endophytic isolates of Muscodor albus, a volatile-antibiotic-producing fungus. Microbiology 150: 4023-4031.   DOI   ScienceOn
25 Fei, Y., H. Ting, J. Z. Wu, B. K. Huang, and L. P. Qin. 2009. Antifungal secondary metabolites from endophytic Verticillium sp. Biochem. Syst. Ecol. 37: 162-165.   DOI   ScienceOn
26 Ganley, R. J., S. J. Brunsfeld, and G. Newcombe. 2004. A community of unknown, endophytic fungi in western white pine. Proc. Natl. Acad. Sci. U.S.A. 101: 10107-10112.   DOI   ScienceOn
27 Goes-Neto, A., C. Loguercio-Leite, and R. T. Guerrero. 2005. DNA extraction from frozen field-collected and dehydrated herbarium fungal basidiomata: Performances of SDS and CTAB-based methods. Biotemas 18: 19-32.
28 Hawksworth, D. C. and A. Y. Rossman. 1987. Where are the undescribed fungi? Phytopathology 87: 888-891.
29 Hershkovitz, M. A., E. A. Zimmer, and W. J. Hans. 1999. Ribosomal DNA sequences and angiosperm systematics, pp. 268-326. In P. M. Hollingsworth, R. M. Bateman, and R. J. Gornall (eds.). Systematics and Plant Evolution. Taylor and Francis, London.