• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2001.05a
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• pp.471-472
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• 2001

Recently many studies show that genetic characters can be used to provide unambiguous taxonomic discrimination when morphological characters are not practical for routine identification to species level (Bucklin et al., 1996, 1998; Lindeque et al., 1999). The internal transcribed spacer (ITS) regions of the nuclear ribosomal DNA are also one of appropriate markers for species-level studies because it contains sufficient diversity to address intra- and interspecific phylogenetic relationships in invertebrates (Odorico and Miller,1997; Schizas et al., 1999). (omitted)

• Animal Systematics, Evolution and Diversity
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• v.36 no.3
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• pp.216-221
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• 2020

Parrots are common targets for illegal trade because of their beauty and high price. Accurate identification is necessary for the prevention of illegal trade and conservation of parrots. In the present study, mitochondrial markers of cytochrome b (CYTB) gene were used to identify parrot species from Korean zoos. Totally, 27 samples were collected from Seoul Zoo, Cheongju Zoo, and Uchi Zoo. After collection, total DNA of samples was extracted and used for PCR amplification. CYTB fragments were sequenced from all samples examined. The obtained sequences were used for GenBank blast, distance estimation, and phylogenetic analysis. All species were identified using CYTB sequences that determined 27 samples belong to 13 species in 7 genera, and 3 families. Our finding demonstrated the usefulness of CYTB sequences for identifying parrot species in Korean zoos.

• The Korean Journal of Mycology
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• v.47 no.3
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• pp.209-218
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• 2019

During a survey of fungal diversity in Korea, two fungal strains, KNU17-1 and KNU17-199, were isolated from paddy field soil in Yangpyeong and Sancheong, respectively, in Korea. These fungal isolates were analyzed based on their morphological characteristics and the molecular phylogenetic analysis of the internal transcribed spacer (ITS) rDNA sequences. On the basis of their morphology and phylogeny, KNU17-1 and KNU17-199 isolates were identified as Albifimbria verrucaria and Deconica coprophila, respectively. To the best of our knowledge, A. verrucaria and D. coprophila have not yet been reported in Korea. Thus, this is the first report of these species in Korea.

• Mycobiology
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• v.49 no.4
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• pp.346-354
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• 2021

The genus Aspergillus is commonly isolated from various marine and terrestrial environments; however, only a few species have been studied in rhizosphere soil. As part of the Korean indigenous fungal excavation project, we investigated fungal diversity from rhizosphere soil, focusing on Aspergillus species. A total of 13 strains were isolated from the rhizosphere soil of three different plants. Based on phylogenetic analysis of β-tubulin and calmodulin and morphological characteristics, we identified five Aspergillus species. A. calidoustus and A. pseudodeflectus were commonly isolated from the rhizosphere soil. Four species were confirmed as unrecorded species in Korea: A. calidoustus, A. dimorphicus, A. germanicus, and A. pseudodeflecuts. The detailed morphological descriptions of these unrecorded species are provided.

• Animal Systematics, Evolution and Diversity
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• v.35 no.1
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• pp.6-9
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• 2019

The genus Dendrodoris Ehrenberg, 1831 includes about 46 valid species worldwide, and is found in relatively shallow waters in the Indo-Pacific, Atlantic, and Australian regions. To date, five Dendrodoris species have been reported from Korea. In this study, we report D. guttata (Odhner, 1917) collected from Jeju Island. Morphology is distinguished from other Dendrodoris species by the shape of the black spots on the dorsal mantle and coloration of the gills. We determined mitochondrial cytochrome c oxidase I (cox1) partial sequences and combined them with publically available sequences of closely related congeneric species to examine its phylogenetic position among Dendrodoris species.

• Journal of Veterinary Science
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• v.19 no.6
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• pp.855-857
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• 2018

Porcine parvovirus 7 (PPV7) was first detected in Korean pig farms in 2017. The detection rate of PPV7 DNA was 24.0% (30/125) in aborted pig fetuses and 74.9% (262/350) in finishing pigs, suggesting that PPV7 has circulated among Korean domestic pig farms. Phylogenetic analysis based on capsid protein amino acid sequences demonstrated that the nine isolated Korean strains (PPV-KA1-3 and PPV-KF1-6) were closely related to the previously reported USA and Chinese PPV7 strains. In addition, the Korean strains exhibit genetic diversity with both insertion and deletion mutations. This study contributes to the understanding of the molecular epidemiology of PPV7 in Korea.

• Animal Systematics, Evolution and Diversity
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• v.35 no.3
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• pp.136-139
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• 2019

The pulmonate gastropod Ellobium chinense (Pfeiffer, 1864) is an endangered marine species along the South Korean coasts due to habitat destruction and population declines. We sequenced the cytochrome c oxidase subunit 1 (COI) of 25 E. chinense specimens collected from five coastal sites in South Korea, and identified 16 unique haplotypes. The maximum intraspecific variation among individuals was 1.6%, while interspecific differences from another ellobiid species, Auriculastra duplicata (Pfeiffer, 1854), ranged from 21.9 to 23.0%. Our barcoding data will be useful to elucidate the phylogenetic relationships among pulmonate gastropods and infer the population genetic structure of E. chinense.

• Mycobiology
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• v.47 no.1
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• pp.1-11
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• 2019

The distribution and occurrence of rare ascomycete fungi within freshwater samples in Korea was investigated. Three rare fungal strains, CNUFC-YR537-1, CNUFC-CNUP1-1, and CNUFC-NDR3-1, were isolated using serial dilution method. On the basis of their morphological characteristics and phylogenetic analysis of their internal transcribed spacer regions and 28S rDNA sequences, the three isolates were identified as Acrostalagmus luteoalbus, Bartalinia robillardoides, and Collariella carteri, respectively. To our knowledge, these are the first records of rare genera Acrostalagmus, Bartalinia, and Collariella from Korea, and the first reports of A. luteoalbus, B. robillardoides, and C. carteri from freshwater samples.

• The Korean Journal of Mycology
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• v.48 no.4
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• pp.491-498
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• 2020

In this study, we aimed to observe the diversity of aquatic fungi by collecting deposits of soil, plants, and plant litter from streams and rivers. Three Penicillium strains were isolated from soil in streams. Based on the morphological characteristics and phylogenetic analysis using DNA sequences of the internal transcribed spacer, β-tubulin and calmodulin genes, the isolates were identified as Penicillium guanacastense, P. saturniforme, and P. scabrosum. These three fungi have not yet been reported in Korea.

• Mycobiology
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• v.49 no.2
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• pp.105-121
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• 2021

Six interesting fungal strains were isolated during a survey of fungal diversity associated with freshwater; these strains were designated as CNUFC YJW2-22, CNUFC MSW11-6-2, CNUFC HRS5-3, CNUFC MSW242-6, CNUFC DMW2-2, and CNUFC CPWS-1. Based on a polyphasic approach including phylogenetic analyses of internal transcribed space (ITS), large subunit (LSU), beta-tubulin (BenA), and calmodulin (CaM) gene sequences, morphological analyses, the six strains were found to be identical to Acremonium guillematii, Cadophora novi-eboraci, Lectera nordwiniana, Mycoarthris corallina, Talaromyces siamensis, and Tetracladium globosum, respectively. To our knowledge, these are the first records of the rare Lectera, Mycoarthris, and Tetracladium genera in Korea, and the first reports of A. guillematii, C. novi-eboraci, L. nordwiniana, M. corallina, T. siamensis, and Te. globosum in a freshwater environment.