• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2003년도 식물바이오벤처 페스티발
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• pp.173-173
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• 2003

• 한국작물학회:학술대회논문집
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• 한국작물학회 2020년도 춘계학술대회
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• pp.31-31
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• 2020

• Journal of Plant Biotechnology
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• 제35권2호
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• pp.95-100
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• 2008

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a main enzyme in the glycolytic pathway, is involved in cellular energy production and regarded as a housekeeping gene. Previously, cytosolic GAPDH was selected as the most significantly abundant gene in EST library of sweetpotato suspension cells. In this study, a full-length of cDNA clone (IbGAPDH) encoding GAPDH was isolated from suspension-cultured cells of sweetpotato (Ipomoea babatas), and its expression was investigated with a view to understanding the physiological function of GAPDH in relation to environmental stresses. IbGAPDH encoded a 36.9 kDa polypeptide consisting of 337 amino acids. When the deduced amino acid of IbGAPDH was compared with other higher plants, IbGAPDH showed high homology with cytosolic GAPDH. The mRNA level of IbGAPDH significantly increased under environmental stresses, such as $H_2O_2$, MV and cold treatments. Among them, the transcript level of IbGAPDH gene was the highest under cold stress. Further investigation of the transcription level under $10^{\circ}C$ or $15^{\circ}C$ was performed with different tissues of sweetpotato. The transcription of IbGAPDH was increased by cold stress with tissue-specificity, moreover, showed different patterns according to temperature.

• Environmental Analysis Health and Toxicology
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• 제13권3_4호
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• pp.133-142
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• 1998

Effects of phosphorous (P) and methylglyoxal (MG) on the cell number, dry weight, chlorophyll content, photosynthetic and respiratory rate, phosphate uptake and protein content of green algae (Scenedesrnus obliquus) were studied. The algal cell number from the medium treated with 0.5-1.0 mM of MG at 1/2 P or 1/4 P concentration was significantly lower than those of algae treated :with full strength of phosphrous in medium. The inhibitory effect of MG on algal cell division was enhenced at low concentration of phosphorous in medium. At the beginning of logrithmic phase of algal growth, the mean dry weight of algae from the medium without MG-treatment in 1/2 P media was significantly higher than that of algae treated with MG. After logrithmic phase of growth cycle, the mean dry weight of algae from the medium with 1.0 mM of MG-treatment in 1/4 P media was significantly lower than that of algae treated with or without MG. At logrithmic phase of algal growth, there were significant differences in the chlorophyll content among all groups of tested algae with various concentrations of P and MG. At 15 days after inoculation, the mean chlorophyll content per algal cell from the media without MG-treatment in 1/2P was significantly higher than that of other cells from MG-treated media. The adverse effect of MG at concentration of 0.5-1.0mM in 1/2 and 1/4 P media on photosynthetic rate was observed. The mean photosynthetic rate of algal cell without P and MG treatment at 15 days after inoculation was significantly higher than that of MGtreated algae. After logarithmic phase, the algal cell treated with 0.5mM of MG with full strength of phosphorous showed significantly high respiratory rate than that of other cell groups. There were significant differences in mean phosphate uptake rate among all groups of Scenedesmus obliquus at logarithmic phase. At 12 days after inoculation, phosphate uptake rate per each algal cell from the basic media without MG and P treatment was rapidly reduced which shows early introduction to stationary phase.

• 대한소아치과학회지
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• 제48권3호
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• pp.359-366
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• 2021

포도당-6-인산탈수소효소 결핍증은 X 염색체 열성으로 유전되는 세계에서 가장 흔한 효소 결핍증이다. 이 질환은 우리나라에서는 드물게 나타나지만, 다문화 가정의 증가로 유병률이 높아질 가능성이 있다. 이 결핍증의 주된 문제는 소아치과에서 일반적으로 사용되거나 처방되는 일부 약물에 의해 용혈성 빈혈이 유발될 수 있다는 점이다. 소아치과 의사는 환자의 병력에 대한 정확한 지식을 갖고 용혈을 유발할 수 있는 산화 스트레스를 피하기 위해 소아 혈액 전문의와 상담해야 한다. 가장 효과적인 치료는 빈혈을 유발할 수 있는 인자에 노출되지 않도록 예방하는 것이다. 이 질환으로 진단받은 환자들에게 위험인자에 대한 적절한 교육은 필수적이다. 이 증례보고는 G6PD 결핍증 환아에서 주의해야 할 약물과 치과적 관리 방법에 대해 논의하고자 하였다.

• The Plant Pathology Journal
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• 제37권1호
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• pp.36-46
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• 2021

Acidovorax citrulli (Ac) is the causal agent of bacterial fruit blotch (BFB) in watermelon, a disease that poses a serious threat to watermelon production. Because of the lack of resistant cultivars against BFB, virulence factors or mechanisms need to be elucidated to control the disease. Glycerol-3-phosphate dehydrogenase is the enzyme involved in glycerol production from glucose during glycolysis. In this study, we report the functions of a putative glycerol-3-phosphate dehydrogenase in Ac (GlpdAc) using comparative proteomic analysis and phenotypic observation. A glpdAc knockout mutant, AcΔglpdAc(EV), lost virulence against watermelon in two pathogenicity tests. The putative 3D structure and amino acid sequence of GlpdAc showed high similarity with glycerol-3-phosphate dehydrogenases from other bacteria. Comparative proteomic analysis revealed that many proteins related to various metabolic pathways, including carbohydrate metabolism, were affected by GlpdAc. Although AcΔglpdAc(EV) could not use glucose as a sole carbon source, it showed growth in the presence of glycerol, indicating that GlpdAc is involved in glycolysis. AcΔglpdAc(EV) also displayed higher cell-to-cell aggregation than the wild-type bacteria, and tolerance to osmotic stress and ciprofloxacin was reduced and enhanced in the mutant, respectively. These results indicate that GlpdAc is involved in glycerol metabolism and other mechanisms, including virulence, demonstrating that the protein has pleiotropic effects. Our study expands the understanding of the functions of proteins associated with virulence in Ac.

• 원예과학기술지
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• 제34권4호
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• pp.587-595
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• 2016

본 연구는 염류-나트륨성 토양 조건에서 작물의 생육에 미치는 퇴비, 석고, 인산의 효과를 평가하기 위해, 2013년과 2014년에 배추를 포트재배하였고, 퇴비와 화학비료를 시비한 표준시비 처리구(S), 표준시비에 퇴비 추가 처리구(S + C), 석고 추가 처리구(S + G), 인산 추가 처리구(S + P), 석고-인산 추가 처리구(S + GP) 등 다섯 처리를 두어 비교하였다. 배추 건물중은 2013년과 비교하여 2014년에 크게 증가하였다. 토양의 전기전도도(EC)가 2013년에 비해 2014년에 감소하지 않았지만, 토양용액내 양이온 중 나트륨의 평균 비율($SAR_{1:5}$)이 2013년 $17.3{\pm}1.1$에서 2014년 $11.2{\pm}2.7$로 크게 감소하였다. 토양개량제 중에서 석고를 시용하였을 때 배추의 생육이 가장 좋았다. 표준시비 처리구와 비교하여 석고 추가 처리구에서 배추 건물중이 2013년에 7.0g/plant(48.2%) 더 높았고 2014년에 7.9g/plant(16.6%) 더 높았다. 석고 시용 시 오히려 토양 전기전도도가 증가하여 배추 생육에 부정적이었지만, SAR이 2013년과 2014년에 각각 14%, 38% 감소하여 배추생육에 긍정적인 효과가 있었다. 본 연구 결과는 염류-나트륨성 토양이 분포하는 간척지에 석고를 시용하여 토양의 나트륨 비율을 개선함으로써 작물의 생육을 촉진시킬 수 있다는 것을 시사해 주었다.

• 한국가금학회지
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• 제37권2호
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• pp.131-137
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• 2010

고온기 때 육계 수송 스트레스를 줄이기 위한 대비책으로 농가에서 출하 2일전에 버퍼제(sodium phosphate, sodium bicarbonate, magnesium sulfate)를 급여함으로 육계의 품질에 미치는 영향을 구명하기 위해 수행하였다 닭고기의 품질 $1^+$ 등급 평가에서 버퍼제를 급여하지 않는 대조구에서 4%를 차지하였으나 sodium phosphate 급여구가 8%, sodium bicarbonate 급여구 14%, magnesium sulfate 급여구가 28%로 대조구에 비하여 버퍼제를 급여한 처리구에서 $1^+$등급 출현율이 증가하였다. PSE 발생율은 대조구에서 경증 및 중증 모두 합하여 92%를 차지하였으나, sodium phosphate 급여구가 88%, sodium bicarbonate 급여구 28%, magnesium sulfate 급여구가 56%로 대조구에 비하여 버퍼제를 급여한 처리구에서 PSE 발생율이 현저히 저하되었다. 닭고기 외관의 멍은 대조구에서는 1~6 cm의 멍이 32%를 차지하였으나, sodium phosphate 급여구는 22%, sodium bicarbonate 급여구 24%, magnesium sulfate 급여구가 44%로 magnesium sulfate 급여구를 제외하고는 대조구에 비해 멍 발생율의 8~10% 정도 저하하였다. 닭고기의 명도($L^*$) 값의 변화는 가슴 근육 부위에서 대조구가 67.88을 나타내었고, sodium phosphate 급여구가 67.05, sodium bicarbonate 급여구 66.27, magnesium sulfate 급여구가 65.89로 magnesium sulfate 급여구에 명도가 가장 낮은 경향을 나타내었고, 전체적으로 대조구에 비해 버퍼제를 급여한 구에서 명도 값이 저하되었다. 상기와 같이 고온기 때 육계 수송 스트레스를 줄이기 위하여 버퍼제(sodium phosphate, sodium bicarbonate, magnesium sulfate)를 급여함으로 사계 및 닭고기의 이상 도체를 줄일 수 있을 것으로 사료된다.

• 한국초지조사료학회지
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• 제42권2호
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• pp.120-126
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• 2022

The objective of this study was to determine effects of phosphorus on lignification and carbohydrate metabolism in Kentucky bluegrass under drought stress. Drought stress was induced by reducing of water to plants in pots. Two types of phosphorus were applied as potassium phosphate (PO₄^3-; P) or potassium phosphonate (PO₃^3-; PA) in drought-stressed plants. Drought had significant negative effects on plant growth, as revealed by reduced biomass of shoot. Drought-induced increase of lignin content was concomitant with the increase of phenylalanine ammonia-lyase (PAL). Soluble sugar content was highly increased but fructan content was largely decreased by drought stress. However, the application of phosphorus was efficient to ameliorate the adverse effects of drought. PA application improved reduced shoot growth and relative water content, and inhibited lignification synthesis with a reduction of PAL activity. P or PA application maintained soluble sugar and fructan content at similar levels to controls under drought stress. These results indicate that phosphorus application may mitigate the drought stress by inhibiting the lignification and promoting the fructan assimilation.

• 한국해양바이오학회지
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• 제6권1호
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• pp.31-40
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• 2014

The production of astaxanthin in the microalga Haematococcus pluvialis has been investigated using a sequential methodology based on the application of two types of statistical designs. The employed preliminary experiment was a fractional factorial design $2^6$ in which the factors studied were: excessive irradiance and nitrate starvation, phosphate deficiency, acetate supplementation, salt stress, and elevated temperature. The experimental results indicate that the amount of astaxanthin accumulation in the cells can be enhanced by excessive irradiance and nitrate starvation whereas the other factors tested did not yield any enhancement. In the subsequent experiment, a central composite design was applied with four variables, light intensity, nitrate, phosphate, and acetate, at five levels each. The optimal conditions for the highest astaxanthin production were found to be $1040{\mu}E/(m^2{\cdot}s)$ light intensity, 0.04 g/L nitrate, 0.31 g/L phosphate, 0.05 g/L acetate concentration.