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Isolation and Characteristics of a Phenol-degrading Bacterium, Rhodococcus pyridinovorans P21 (페놀분해세균 Rhodococcus pyridinovorans P21의 분리 및 페놀분해 특성)

  • Cho, Kwang-Sik;Lee, Sang-Mee;Shin, Myung-Jae;Park, Soo-Yun;Lee, Ye-Ram;Jang, Eun-Young;Son, Hong-Joo
    • Journal of Life Science
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    • v.24 no.9
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    • pp.988-994
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    • 2014
  • The effluents of chemical and petroleum industries often contain non-biodegradable aromatic compounds, with phenol being one of the major organic pollutants present among a wide variety of highly toxic organic chemicals. Phenol is toxic upon ingestion, contact, or inhalation, and it is lethal to fish even at concentrations as low as 0.005 ppm. Phenol biodegradation has been studied in detail using bacterial strains. However, these microorganisms suffer from substrate inhibition at high concentrations of phenol, whereby growth is inhibited. A phenol-degrading bacterium, P21, was isolated from oil-contaminated soil. The phenotypic characteristics and a phylogenetic analysis indicated the close relationship of strain P21 to Rhodococcus pyridinovorans. Phenol biodegradation by strain P21 was studied under shaking condition. The optimal conditions for phenol biodegradation by strain P21 were 0.09% $KNO_3$, 0.1% $K_2HPO_4$, 0.3% $NaH_2PO_4$, 0.015% $MgSO_4{\cdot}7H_2O$, 0.001% $FeSO_4{\cdot}7H_2O$, initial pH 9, and $20-30^{\circ}C$, respectively. When 1,000 ppm of phenol was added to the optimal medium, the strain P21 completely degraded it within two days. Rhodococcus pyridinovorans P21 could grow in up to 1,500 ppm of phenol as the sole carbon source in a batch culture, but it could not grow in a medium containing above 2,000 ppm. Moreover, strain P21 could utilize toxic compounds, such as toluene, xylene, and hexane, as a sole carbon source. However, no growth was detected on chloroform.

Fenton-like Reaction for Treatment of Petroleum-Contaminated Silty Clay after Soil Washing Process (토양세척 후의 유류 오염 Silty Clay 처리를 위한 유사펜톤 산화반응)

  • So, Myung-Ho;Ha, Ji-Yeon;Yu, Jae-Bong;Kim, Chang-Gyun
    • Journal of Korean Society of Environmental Engineers
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    • v.31 no.1
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    • pp.1-8
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    • 2009
  • This research was performed to assess a Fenton-like oxidation using naturally present iron in the field to treat remained oils throughout silty clay residues which finally resided even after a series of soil washing process. Biodegradability was thus tested for reaction products to investigate a possible treatment of the Fenton-like oxidation coupled with a biological treatment process. For those purposes, two types of field soil samples (e.g., dewatered cake after conditioning with a polymer and not-dewatered residue) were tested to remove TPH by adding the various concentration of hydrogen peroxide ($H_2O_2$). Moreover the biodegradability of treated samples was observed based on the ratio of $BOD_5/COD_{Cr}$ after Fenton-like oxidation. The Highest removal of TPH was at 1% of hydrogen peroxide ($H_2O_2$) when hydrogen peroxide ($H_2O_2$) was continuously injected for a period of time rather than that of spot introduction with the same amount of it. For the dewatered cake, TPH was effectively treated when the ratio of solid and water was mixed at 1 : 2. Employing cooking oil could increase solubility of TPH due to enhanced surface-active escalating TPH desorption from silty clay. Nonetheless, the biodegradability was decreased as long as the oxidation duration being extended regardless of operational conditions. It was therefore proved that Fenton-like oxidation using $H_2O_2$ and natural iron minerals was able to remove adsorbed oils in silty clay but the removal efficiency of TPH was low. And if a biological treatment process followed after Fenton-like oxidation, microorganisms would need enough time for acclimation.

Development and Application of Okara-based Adhesives for Plywood Panels (두부비지를 이용한 합판용 접착제의 개발 및 적용)

  • Oh, Sei-Chang;Ahn, Sye-Hee;Choi, In-Gyu;Jeong, Han-Seob;Yoon, Young-Ho;Yang, In
    • Journal of the Korean Wood Science and Technology
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    • v.36 no.3
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    • pp.30-38
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    • 2008
  • Petroleum-based resin adhesives have extensively been used for the production of wood panels. However, with the increase of manufacturing cost and the environmental issue, such as the emission of volatile organic compounds, of the adhesive resins, it is necessary to be developed new adhesive systems. In this study, the potential of okara, which is a residue wasted from the production of tofu, for the development of bio-based adhesives was investigated. At first, the physical and chemical properties of okara were examined. After okara was hydrolyzed in acidic and/or alkaline solutions, okara-based adhesive resins were formulated with the mixtures of the okara hydrolyzates and phenol formaldehyde (PF) prepolymer. The adhesive resins were used for the fabrication of plywood panels, and then the adhesive strength and formaldehyde emission of the plywood panels were measured to examine the applicability of the resin adhesives for the production of plywood panels. The solids content and pH of the okara used in this study were around 20% and weak acidic state, respectively. In the analysis of its chemical composition, the content of carbohydrate was the highest, and followed by protein. The shear strengths of plywood fabricated with okara-based resin adhesives exceeded a minimum requirement of KS standard for ordinary plywood, but its wood failure did not reach the minimum requirement. In addition, the formaldehyde emissions of all plywood panels were higher than that of E1 specified in the KS standard. Based on these results, okara has the potential to be used as a raw material of environmentally friendly adhesive resin systems for the production of wood panels, but further researches - biological hydrolysis of okara and various formulations of PF prepolymer - are required to improve the adhesive strength and formaldehyde emission of okara-based resin adhesives.

A Study of on the Method to Select Manufacturing Activities Sensitive to Regional Characteristics by Analyzing the Locational Hierarchy (입지계층분석을 활용한 산업단지 유치 업종 결정에 관한 연구)

  • So, Jin-Kwang;Lee, Hyeon-Joo;Kim, Sun-Woo
    • Land and Housing Review
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    • v.2 no.4
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    • pp.559-568
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    • 2011
  • This study aims at listing up those manufacturing activities sensitive to regional characteristics by analyzing locational hierarchy designed on the urban rank-size rule. This locational hierarchy by manufacturing activities is expected to provide a ground for the proper supply of an industrial complex. The analysis of the locational hierarchy by manufacturing activities can work as a method of observing the characteristics of the distribution of location for each economic activity by analyzing the trend in the change of manufacturing location. Consequently, it can be used to determine the appropriate manufacturing activities for the industrial complex of a particular region. Here, the locational hierarchy is analyzed depending on the base of the basic local government such as Gun(district level) and Si(city level), and manufacturing activities are categorized by Korea Standard Industry Code. Those activities demonstrating growth pattern are Manufacture of Electronic Equipment(KSIC 26), Manufacture of Medical Precision Optical Instruments Watch(KSIC 27), Manufacture of Motor Vehicles (KSIC 30, 31), etc. With proper infrastructures, these activities can be located everywhere. Those sectors on the decline pattern in the locational hierarchy can be summarized as Manufacture of Tobacco Products(KSIC 12), Manufacture of wearing apparel Fur Articles(KSIC 14), etc. Those sectors scattered widely in the locational hierarchy are Manufacture of Food Products(KSIC 10), Manufacture of Coke Petroleum Products(KSIC 19), Manufacture of Chemical Products(KSIC 20), Manufacture of Electronic Equipment(KSIC 26). These particular manufacturing activities can be operated in those regions in a sufficient supply of unskilled workers regardless of proper infrastructures. Those activities that have a tendency to reconcentrate on larger cities are Manufacture of Textiles(KSIC 13), Manufacture of Wearing Apparel Clothing Fur Articles(KSIC 14), Manufacture of Other Transport Equiptmen(KSIC 31). In most cases, these sectors tend to favor their existing agglomerated areas and concentrate around large cities. Therefore, it is inefficient to promote these sectors in small or medium-sized cities or underdeveloped regions. The establishment of developmental strategies of an industrial complex can gain greater competitiveness by observing such characteristics of the locational hierarchy.

New demand forecast for vocational high school graduates in regional strategic industries: Focusing on comparison between Daejeon and Jeonnam (지역전략산업에 따른 특성화고 졸업자 신규수요 예측: 대전과 전남 지역 비교를 중심으로)

  • Kim, Jin-Mo;Choi, Su-Jung;Jeon, Yeong-Uk;Oh, Jin-Ju;Ryu, Ji-Eun;Kim, Seon-Geun
    • Journal of vocational education research
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    • v.36 no.1
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    • pp.47-75
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    • 2017
  • The purpose of this study was to provide basic data for policy making for secondary vocational education in each region and transformation in vocational high schools. To achieve this, the regional strategic industries in Daejeon and Jeonnam were selected, new demand for vocational high school graduates was forecasted in each industry and occupation. The results of the study are as follows. First, locational quotient analysis and regional shift-share analysis revealed that Daejon and Jeonnam have different strategic industries. Daejon, unlike Jeonnam strategically develops 'manufacturing food, beverage and tobacco', 'manufacturing timber and paper, printing and copying', 'public service and administration of national defense and social security' and 'manufacturing electrical devices, electronics and precision devices'. Jeonnam has specialized industries distinguished from Daejon's, which are 'manufacturing of machinery transportation equipments and etc', 'manufacturing of non-metallic minerals and metal products', 'electric, gas, steam and water supply systems/industries', 'manufacturing coal and chemical products, refining petroleum', 'mining' and 'agriculture, forestry and fishery'. Second, new demand for vocational high school graduates by occupations and industries showed regional differences(in Daejon and Jeonnam). According the forecast, Daejon will have many workforce demands based on manufacturing industries, on the other hand Jeonnam's focused on service industries. Analysis by occupations was also different, Daejon showed high demands on professional and related workers, while Jeonnam requested many new office and service workers. Third, new workforce demand by occupations in regional strategic industries is big part of overall new workforce demand both in Daejon and Jeonnam. Forth, according to the results of analyzing the new demand for vocational high school graduates in Daejeon and Jeonnam in terms of industry location quotient and change effect, there was high demand in industries with positive total change effects. In terms of location quotient, Daejeon and Jeonnam showed different results.

A Study on Status Analysis for Advancement iNto Agricultural Sector in Central Asia (중앙아시아 농업분야 진출을 위한 현황분석 - 우즈베키스탄, 카자흐스탄, 키르기즈스탄 중심으로 -)

  • Park, Dong-Jin;Jo, Sung-Ju;Park, Jeong-Woon;Sa, Soo-Jin;Hong, Jung-Sik;Lee, Dong-Jin
    • Journal of the Korean Society of International Agriculture
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    • v.30 no.4
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    • pp.328-338
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    • 2018
  • Central Asia (Uzbekistan, Kazakhstan, Kyrgyzstan) is a hot and arid continental climate, with most areas (68%) consisting of barren vegetation, desert, and meadows. The main agricultural areas for crop production include irrigated farmland, non-irrigated farmland, grassland, prairie and mountain. We are experiencing climate change with recent climate variability increasing. Agriculture is one of major economic sectors and provides a means of livings for the rural population of Central Asia, especially the poor. In the past two decades, Central Asia has experienced a high population growth rate, with Kazakhstan at 16.8%, Uzbekistan at 34.5% and Kyrgyzstan at 28.4%. As a major industry, Kazakhstan has the largest share of exports of agricultural products followed by petroleum, mineral resources, steel, and chemicals. Uzbekistan is the fifth largest cotton exporter as well as the sixth largest cotton producer in the world. Kyrgyzstan exports ores, stones, cultured pearls, and minerals. These three countries are rich in mineral resources, agricultural products, and energy resources. However, not only do they have difficulties in economic development due to the weakness of logistics and industrial infrastructure, but they also have imperceptible cooperation and investment among countries due to insufficient research and development. Through this study, we will investigate national outlook, economic indicators, major agricultural products, import and export status, and agricultural technology cooperation status, and study how Korean agricultural industry advances into these countries through SWOT analysis. Through this, we hope to contribute to the basic data of Central Asian studies and cooperation and investment in agriculture in each country. In addition, in order to increase cooperative exchange and investment in these countries, we will prepare a Central Asia logistics hub for the rapidly changing interKorean railroad era.

Assessment of the Effect of Dimethyl Ether (DME) Combustion on Lettuce and Chinese Cabbage Growth in Greenhouse (온실에서 상추와 배추를 이용한 DME 원료 난방 효율분석)

  • Basak, Jayanta Kumar;Qasim, Waqas;Khan, Fawad;Okyere, Frank Gyan;Lee, Yongjin;Arulmozhi, Elanchezhian;Park, Jihoon;Cho, Wonjun;Kim, Hyeon Tae
    • Journal of Bio-Environment Control
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    • v.28 no.4
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    • pp.293-301
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    • 2019
  • The experiment was conducted to determine the performance of DME combustion gas when used as a fuel for DME burner for raising temperature and $CO_2$ concentration in greenhouse and also to examine its effects on chlorophyll content, and fresh and dry weight of lettuce and Chinese cabbage. DME-1 and DME-2 treatments consisted of average DME flow quantity in duct were $17.4m^3min^{-1}$ and $10.2m^3min^{-1}$ respectively to greenhouse-1 and greenhouse-2 and no DME gas was supplied to greenhouse-3 which was left as control (DME-3). DME supply times were $0.5hr\;day^{-1}$, $1hr\;day^{-1}$, $1:30hrs\;day^{-1}$ and $2hrs\;day^{-1}$ on week 1, 2, 3, and 4 respectively. Chlorophyll content and fresh and dry weight of lettuce and Chinese cabbage were measured for each treatment and analyzed through analysis of variance with a significance level of P<0.05. The result of the study showed that $CO_2$ concentration increased up to 265% and 174% and the level of temperature elevated $4.8^{\circ}C$ and $3.1^{\circ}C$ in greenhouse-1 and 2, respectively as compared to greenhouse-3 due to application of DME combustion gas. Although, the same crop management practices were provided in greenhouse-1, 2 and 3 at a same rate, the highest change (p<0.05) of chlorophyll content, fresh weight and dry weight were found from the DME-1 treatment, followed by DME-2. As a result, DME combustion gas that raised the level of temperature and $CO_2$ concentration in the greenhouse-1 and greenhouse-2, might have an effect on growth of lettuce and Chinese cabbage. At end of experiment, the highest fresh and dry weight of lettuce and Chinese cabbage were measured in greenhouse-1 and followed by greenhouse-2. Similarly chlorophyll content of greenhouse-1 and greenhouse-2 were more compared to greenhouse-3. In general, DME was not producing any harmful gas during its combustion period, therefore it can be used as an alternative to conventional fuel such as diesel and liquefied petroleum gas (LPG) for both heating and $CO_2$ supply in winter season. Moreover, endorsed quantify of DME combustion gas for a specified crop can be applied to greenhouse to improve the plant growth and enhance yield.

A Study on the Flammability and Combustion Risk of Biodiesel Mixture (바이오디젤 혼합물의 인화 및 연소 위험성에 관한 연구)

  • Kim, Ju Suk;Ko, Jae Sun
    • Journal of the Society of Disaster Information
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    • v.17 no.1
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    • pp.10-24
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    • 2021
  • Purpose: The purpose of this study is to determine the dangers of biodiesel and general diesel mixtures currently used as alternative fuels by equipment (tag method and penski Marten method) and to determine the difference between flash point and combustion point (closed, open) according to test methods. It is intended to be used as a reference material for identification and evaluation of firecausing substances by confirming the risk of mixtures by comparative analysis and measurement, and establishing a risk assessment method for chemical substances. Method: Flash point test method and result treatment were tested based on ASTM and KS M mode, which are tag sealing and pen schematense test methods used as flash point and combustion point test methods for crude oil and petroleum products. The manufacturer of the equipment used in this experiment was a test equipment that satisfies the test standards of KS M 2010 with equipment produced by TANAKA of Japan. The flash point and combustion point were measured, and the flash point according to the test method of biodiesel and general diesel mixture ( Closed, open), and the ignition point of a mixture of biodiesel and general diesel was compared and analyzed for ignition risk compared with conventional diesel. Results: Looking at the experimental results, first, as an analysis of the risk of flammability of the mixture, the flash point of a substance containing 70% biodiesel was found to be about 92℃ based on general diesel with a flash point of 64.5℃, and gasoline and biodiesel or When the biodiesel mixture was synthesized, it was confirmed that the flash point tends to decrease. In addition, the difference between the flash point and the combustion point was analyzed as about 20 ~ 30℃, and when a small amount of gasoline or methanol was mixed, the flash point was lowered, but it was confirmed that the combustion point was similar to that of the existing mixture. Conclusion: In this study, in order to secure the effectiveness of the details of the criteria for judging dangerous materials in the existing Dangerous Materials Safety Management Act, and to secure the reliability and reproducibility of the judgment of dangerous materials, we confirm the criteria for judging the risk of the mixture through an experimental study on flammable mixtures. It will be able to provide reference data for experimental criteria for flammable liquids that are regulated in the field. In addition, if this study accumulates know-how on experiment by test method, it is expected that it can be used as a basis for research on risk assessment and research on dangerous goods.

A Study on Flammability Risk of Flammable Liquid Mixture (가연성 액체 혼합물의 인화 위험성에 관한 연구)

  • Kim, Ju Suk;Koh, Jae Sun
    • Journal of the Society of Disaster Information
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    • v.16 no.4
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    • pp.701-711
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    • 2020
  • Purpose: In this study, the risk of flammability of a liquid mixture was experimentally confirmed because the purpose of this study was to confirm the increase or decrease of the flammability risk in a mixture of two substances (combustible+combustible) and to present the risk of the mixture. Method: Flash point test method and result processing were tested based on KS M 2010-2008, a tag sealing test method used as a flash point test method for crude oil and petroleum products. The manufacturer of the equipment used in this experiment was Japan's TANAKA. The flash point was measured with a test equipment that satisfies the test standards of KS M 2010 with equipment produced by the company, and LP gas was used as the ignition source and water as the cooling water. In addition, when measuring the flash point, the temperature of the cooling water was tested using cooling water of about 2℃. Results: First of all, in the case of flammable + combustible mixtures, there was little change in flash point if the flash point difference between the two substances was not large, and if the flash point difference between the two substances was low, the flash point tended to increase as the number of substances with high flash point increased. However, in the case of toluene and methanol, the flash point of the mixture was lower than that of the material with a lower flash point. Also, in the case of a paint thinner, it was not easy to predict the flash point of the material because it was composed of a mixture, but as a result of experimental measurement, it was measured between -24℃ and 7℃. Conclusion: The results of this study are to determine the risk of mixtures through experimental studies on flammable mixtures for the purpose of securing the effectiveness of the details of the criteria for determining dangerous goods in the existing dangerous goods safety management method and securing the reliability and reproducibility of the determination of dangerous goods Criteria have been presented, and reference data on experimental criteria for flammable liquids that are regulated in firefighting sites can be provided. In addition, if this study accumulates know-how on differences in test methods, it is expected that it can be used as a basis for research on risk assessment of dangerous goods and as a basis for research on dangerous goods determination.

The Influence of the Characteristics of Drainage Basin on Depositional Processes of the Alluvial Fan: An Example from the Cretaceous Duwon Formation in Goheung Area (유역분지 특성에 따른 충적선상지의 퇴적작용: 고흥군 백악기 두원층의 예)

  • Lee, Kyung Jin;Park, Seung-Ik;Lee, Hyojong;Gihm, Yong Sik
    • Economic and Environmental Geology
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    • v.54 no.4
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    • pp.441-456
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    • 2021
  • The Cretaceous Duwon Formation was studied on the basis of sedimentologic analysis in order to unravel geologic conditions for the development of the streamflow-dominated alluvial fan under arid to semi-arid climatic conditions. The Duwon Formation unconformably overlies the Paleoproterozoic gneiss (basement). Based on the sedimentologic analysis, the Duwon Formation is interpreted to have been deposited in gravelly braided stream (FA-1) near the basement, laterally transitional to sandy braided stream (FA-2) and floodplain environments (FA-3) with distance (< 7 km) from the basement. Lateral changes in sedimentary facies and the well development of calcrete nodules in FA-3, together with radial paleocurrent directions measured in FA-1, are suggestive of the deposition of the Duwon Formation in streamflow-dominated alluvial fan under arid to semi-arid climatic conditions. Recent analysis of detrital zircon chronology suggests that sediments of the Duwon Formation were derived from the southwestern part of the Korean peninsula, including the western part of Yeongnam Massif and the southwestern part of Okcheon Belt. This implies the alluvial fan where the Duwon Formation accumulated had the large drainage basin. Because the large drainage basin can supply the significant amounts of water and temporarily store the sediments within the basin, watery floodwater carried sediments to the alluvial fan rather than the debris flows. Furthermore, the drainage basin largely composed of coarse-grained metamorphic and igneous rocks produced sand-grade sediments, preventing evolution of floodwater into debris flows. We suggest that combined effects of the large drainage basin and its coarse-grained metamorphic and igneous rocks provided favorable conditions for the development of streamflow-dominated alluvial fan, despite arid to semi-arid climatic conditions during sedimentation.