• Journal of Bio-Environment Control
• /
• v.8 no.1
• /
• pp.19-29
• /
• 1999

This experiment was conducted to compare with the growth and fruit quality of Citrullus lanatus cv. Mudeungsan and Citrullus vulgaris cv. Dalgona grown in fertigation and soilless culture. Seeds were sown on April 16 and transplanted on April 24, 1998. Cultural systems used in fertigation and soilless culture beds using mixed substrate, coir dust (50%) and perlite (50%). In the plot of fertigation system, the number of leaves of cv. Dalgona were increased more than that of cv Mudeungsan, but leaf area shown inversed trend. Plant growth shown greater in soilless culture than those of fertigation culture. In regardless of cultural systems, soluble solid content in fruit was higher in the cv. Dalgona than that of cv. Mudeungsan, but fruit fresh weight was greater in cv. Mudeungsan compare to the cv Dalgona. Nitrate content in petiole sap of watermelon in regardless of cultivars and cultural systems was 11.4∼13.4mg/gFW on 15 days after transplanting, and then increased to 17.1∼20.6mg/gFW on the fruit growth stage. Phosphorous content was 3.7∼5.7mg/gFW in the early growth stage while decreased to 0.6∼1.1mg/gFW from maturing stage to harvesting stage. Potassium content was increased to 5.8∼6.6mg/gFW in the early growth stage while decreased to 4.0∼4.8mg/gFW from pollination stage to harvesting stage. Calcium content in spa petiole of watermelon was higher in soilless culture as 3.4∼4.1mg/gFW than 2.5∼3.5mg/gFW of fertigation culture, but calcium content in fertigation culture during maturing stage was higher than that of soilless culture. The tendency of magnesium uptake was higher in fertigation culture than that of soilless culture, and was similarly absorbed in the range of 0.9∼1.3mg/gFW in regardless of cultural method after pollination. It was demonstrated that cv. Mudeungsan can be adapted to soilless culture and improved the fruit quality. Consequently, hydroponic possibility for year round culture in the greenhouse was recognized.

• Journal of Bio-Environment Control
• /
• v.30 no.4
• /
• pp.351-358
• /
• 2021

The study was investigated to compare growth characteristics, the antioxidant activity, and the triterpenoid content of two Centella asiatica cultivars ('Giant Tiger Care' and 'Good Tiger Care'). At 41 days after transplanting, lengths of leaf and petiole were significantly longer in 'Good Tiger Care' than in 'Giant Tiger Care'. However, the growth characteristics (leaf area, petiole thickness, petiole length, and weight) were greater in 'Giant Tiger Care' than in 'Good Tiger Care' at 104 days after transplanting (harvest time). Antioxidant activity and total phenol content in four extracts (WE, water extract; HWE, hot water extract; 50E, 50% EtOH extract; 70E, 70% EtOH extract) of the two cultivars were high in 70E of 'Giant Tiger Care'. As a result of the triterpenoids analysis, the major triterpenoids of the two cultivars were identified as madecassoside and asiaticoside. The total triterpenoid content was high in 50E and 70E of 'Giant Tiger Care' and 'Good Tiger Care', respectively but the total triterpenoid content was highest in Good Tiger Care'. However, at the 104 days after planting, the yield of 'Giant Tiger Care' was three times higher than that of 'Good Tiger Care'. In addition, the total triterpenoid content that can be produced in the same cultivation area (3.3m²) was 2.459mg in 50E of 'Giant Tiger Care', which was ~2.2times higher than that of 70E of 'Good Tiger Care' (1.103 mg). Thus, it is considered economical to cultivate 'Giant Tiger Care' which has the highest antioxidant activity and high total triterpenoid production per cultivation area.

• Korean Journal of Plant Resources
• /
• v.18 no.3
• /
• pp.367-373
• /
• 2005

The purposes of this dissertation were to examine relativity between growth characteristics factors and develop the model of growth characteristics. In the result of statistical summaries of the factors in growth characteristic, the highest level of correlation was in plant height, plant width, petiole and chlorophyll in the case of Artemisia iwayomogi; leaf length, in the case of A. stolonifera;, leaf width, of A. princeps var. orientalis;, diameter, of A. feddei;, leaf thickness, of A. iwayomogi, A. princeps var. orientalis, A. montana, A. stolonifera, A. sylnatica; leaf length and leaf width of the other species. In the case of correlation analysis, leaf width and diameter were most correlative in A. capillaris;, plant height and plant width, in A. montana; plant height and leaf width, in A. stolonifera; leaf length and leaf width, in other species. The results of model analysis of growth characteristics were as follows. A. capillaris proved to be about $79.4\%$ effective ; A. princeps var. orientalis, about $91.7\%$ ; A. montana, about $70.3\%$ ; A. iwayomogi, about $49.0\%$ ; A. stolonifera, about $72.5\%$; A. sylnatica, about $75.0\%$;, A. feddei about $60.4\%$ ; A. rubripes about $54.0\%$.

• Journal of Plant Biotechnology
• /
• v.47 no.4
• /
• pp.298-308
• /
• 2020

Genetic diversity among Thaumatococcus daniellii populations in the southwestern region of Nigeria were assessed using morphometric and molecular markers to determine the population structure and existing genetic relationship for its improvement, conservation and sustainable utilisation. Populations from five locations in each of the six states were used for the study. Morphometric data were collected on folia characters and analysed for variability. Genome DNA was isolated from the plant leaf and amplified by polymerase chain reaction with inter-simple sequence repeat markers (ISSR) to determine the allelic polymorphism, marker effectiveness and genetic relationship of the population. The results showed significant variations in petiole length and leaf dimensions of the populations within and across the states. These morphometric traits are the major parameters that delimit the populations and they correlated significantly at P≤0.05. Analysis of the electrophoregram showed that the ISSR markers are effective for the diversity study. A total of 136 loci were amplified with an average of 7.16 loci per marker, 63.2% of the loci were polymorphic. The Principal Coordinate Analysis revealed that seven factors accounted for 81.6% of the variation and the dendrogram separated the populations into two major groups at a genetic distance of 10 (about 90% similarity) with sub-groups and clusters. Most populations within the state had a high degree of similarity, nonetheless, strong genetic relationship exists among populations from different states. The close relationship between populations across the states suggests a common progenitor, which are likely separated by ecological or geographical isolation mechanisms.

• Journal of Korean Society of Forest Science
• /
• v.59 no.1
• /
• pp.1-8
• /
• 1983

Classification on the group of Salix pseudo-lasiogyne growing in Korea, was conducted using cluster analysis, factor analysis, and principal component analysis. Thirty-six characters(Table 2) of the 5 basis species were measured. The phenograms and ordination plot showing the relationships between the species were made by applying the cluster analysis and principal component analysis. Five important factors, such as leaf blade width, number of right serration, angle of leaf apex, number of flowers in an ament, and the ratio of petiole length to width, were inferred from the rotated factor matrix, and their state values were presented in polygonal diagram. Salix pseduo-lasiogyne and S. babylonica were similarly correlated and linked in one group, S. dependens and S. matsudana for tortuosa were secondarily linked in the other group. S. koreensis appeared as an aliemated species from each of the two groups.

• Korean Journal of Plant Resources
• /
• v.19 no.1
• /
• pp.105-111
• /
• 2006

The objectives of this study, an analysis of fruit and leaf morphological characteristics among the five Zizyphus cultivars could be used for the investigation of cultivars classification and could provide information to make out the UPOV TG(Test Guidelines). ANOVA tests showed that there were statistically significant differences in all fruit and leaf morphological characteristics among the five Zizyphus cultivars at 1% level. But, for kernel characteristics, differences were statistically non-significant among the cultivars. Approximately, the Wolchul and Boeun cultivars showed larger and smaller values in overall characteristics and cultivars, respectively. The results of principal component analysis(PCA) for the fruit and leaf morphological characteristics showed that the first for principal components(PC's) explained about 65.3% of the total variation. The first PC was correlated with those characteristics that were mainly related to the terminal leaf length(TLL), leaf length(LL), fruit length(FL), terminal leaf width(TLW), and leaf petiole length(LPL). The second and third PC was mainly correlated with the terminal leaf morphological index(TLMI). Therefore, these characteristics were important to analysis of the fruit and leaf morphological characteristics and classification among the five Zizyphus cultivars. Cluster analysis using UPGMA method based on principal components showed that five Zizyphus cultivars could be clustered into two groups. Group I comprises Mudung, Wolchul, and Bokjo and Geumsung cultivars, Group II is Boeun cultivar. These results well similar to that of principal component analysis.

• Journal of Plant Biotechnology
• /
• v.31 no.4
• /
• pp.267-271
• /
• 2004

Transformed sweetpotato (Ipomoea batatas (L.) Lam. cv. Yulmi) plants were developed from embryogenic calli following Agrobacterium tumefaciens-mediated transformation. A. tumefaciens strain EHA105/pCAMBIA2301 harboring genes for intron $\beta$-glucuronidase (GUS) and kanamycin resistance. Transient expression of GUS gene was found to be higher when embryogenic calli were co-cultivated with Agrobacterium for 2 days. The co-cultured embryogenic calli transferred to selective MS medium containing 1mg/L 2,4-D, 100mg/L kanamycin, and 400mg/L claforan. These embryogenic calli were subcultured to the same selection medium at 4 weeks interval. Kanamycin-resistant calli transferred to hormone-free MS medium with kanamycin gave rise to somatic embryos and then converted into plantlets in the same medium. Southern blot analysis confirmed that the GUS gene was inserted into the genome of the sweetpotato plants. A histochemical assay revealed that the GUS gene was preferentially expressed in the leaf, petiole, and vascular tissue and tip of root.

• Korean Journal of Plant Resources
• /
• v.24 no.5
• /
• pp.540-548
• /
• 2011

Characteristics of leaf morphology was examined in 11 natural populations of Acer pictum subsp. mono (Maxim.) Ohashi in Korea. Nested Anova showed that there were statistically significant differences among populations as well as among individuals within populations in all 14 quantitative characters. In all the leaf characters, variance components among individuals within populations were higher than those among populations. Some morphological characters such as the ration of Blade length to Petiole length (0.90), Uower lobe width (0.85), Blade length (0.83), and Middle lobe width (0.83) showed the relative high differences in the values of differentiation (Dij) among populations. Of the ten populations, Gwangyang (1.36) and Cheongyang (1.30) were highly differentiated based on the total mean Dij of fourteen morphological characters, while Ulju (0.30) and Jeongseon (0.44) were scarcely differentiated.

• Korean Journal of Plant Tissue Culture
• /
• v.25 no.6
• /
• pp.525-530
• /
• 1998

These studies were carried out to select the active grow hairy root lines induced from various ginseng(Panax ginseng C. A. Meyer) parts. Hairy roots were induced in root explants, stem and petiole in vitro by A. rhizogenes R1000 or A. rhizogenes $A_4$. These hairy roots could be grown on the phytohormone free medium, and PCR analysis of rol C and vir C gene fragments confirmed that hairy roots were transgenic tissues. We have selected 11 hairy root lines with active growing characters among 300 hairy root lines selected based on growth and morphological characteristics on 1/2MS solid media with 250 mg/L carbenicillin. Morphological characteristics of selected 11 hairy root lines were thickness and thiness of main roots, and many projection for lateral roots, active grow of lateral roots. Among selected 11 hair root lines prominent characteristics of hairy roots with active growing characters were thiness of main roots and active grow of lateral roots. But characteristics of low growing hairy roots were thickness of main roots and low grow of lateral roots. Finally we have selected actively growing hairy roots, KGHR-1, KGHR-5, KGHR-8 among 11 hairy root lines.

• Korean journal of food and cookery science
• /
• v.14 no.5
• /
• pp.541-546
• /
• 1998

Volatile flavor components in leaf and petiole of fresh Pleurospermum kamtschaticum H$\_$OFFM/ were extracted by SDE (simultaneous steam distillation and extraction) method using diethyl ether as solvent. Essential oils were analyzed by gas chromatography (GC) and combined gas chromatography-mass spectrometry (GC-MS). Identification of volatile flavor components was based on the Rl of GC and mass spectrum of GC-MS. A total of 31 components, including 15 hydrocarbons, 4 aldehydes, 1 ketone, 5 alcohols, 2 esters, 3 acids and 1 oxide were identified in the essential oils. (Z)-${\beta}$-Farnesene, (Z, E)-${\alpha}$-farnesene and farnesene were the major volatile flavor components in fresh Pleurospermum kamtschaticum. Volatile flavor patterns of Pleurospermum kamtschaticum were analyzed using electronic nose. Sensor T30/1 and PA2 that were sensitive to alcohols had the highest resistance for fresh Pleurospermum kamtschaticum. Resistance of six metal oxide sensors was decreased in dried sample compared with fresh one.