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http://dx.doi.org/10.12791/KSBEC.2021.30.4.351

Comparison of Growth Characteristics and Physiological Activity of Two Centella asiatica Cultivars in Greenhouse Soil Culture  

Oh, Sewon (Division of Animal, Horticultural and Food Sciences, Chungbuk National University)
Park, Sujeong (Department of Horticultural Science, Gyeongsang National University)
Lee, Seongho (Department of Horticultural Science, Gyeongsang National University)
Park, Yeonju (Department of Horticultural Science, Gyeongsang National University)
Jang, Keum-Il (Department of Food Science and Biotechnology, Chungbuk National University)
Yu, Kwang-Won (Department of Foold and Nutrition, Korea University of Transportation)
Kim, Daeil (Department of Horticultural Science, Chungbuk National University)
Shin, Hyunsuk (Department of Horticultural Science, Gyeongsang National University)
Publication Information
Journal of Bio-Environment Control / v.30, no.4, 2021 , pp. 351-358 More about this Journal
Abstract
The study was investigated to compare growth characteristics, the antioxidant activity, and the triterpenoid content of two Centella asiatica cultivars ('Giant Tiger Care' and 'Good Tiger Care'). At 41 days after transplanting, lengths of leaf and petiole were significantly longer in 'Good Tiger Care' than in 'Giant Tiger Care'. However, the growth characteristics (leaf area, petiole thickness, petiole length, and weight) were greater in 'Giant Tiger Care' than in 'Good Tiger Care' at 104 days after transplanting (harvest time). Antioxidant activity and total phenol content in four extracts (WE, water extract; HWE, hot water extract; 50E, 50% EtOH extract; 70E, 70% EtOH extract) of the two cultivars were high in 70E of 'Giant Tiger Care'. As a result of the triterpenoids analysis, the major triterpenoids of the two cultivars were identified as madecassoside and asiaticoside. The total triterpenoid content was high in 50E and 70E of 'Giant Tiger Care' and 'Good Tiger Care', respectively but the total triterpenoid content was highest in Good Tiger Care'. However, at the 104 days after planting, the yield of 'Giant Tiger Care' was three times higher than that of 'Good Tiger Care'. In addition, the total triterpenoid content that can be produced in the same cultivation area (3.3m2) was 2.459mg in 50E of 'Giant Tiger Care', which was ~2.2times higher than that of 70E of 'Good Tiger Care' (1.103 mg). Thus, it is considered economical to cultivate 'Giant Tiger Care' which has the highest antioxidant activity and high total triterpenoid production per cultivation area.
Keywords
asiatic acid; asiaticoside; madecassic acid; madecassoside;
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1 Kiselova Y., D. Ivanova, T. Chervenkov, D. Gerova, B. Galunska, and T. Yankova 2006, Correlation between the in vitro antioxidant activity and polyphenol content of aqueous extracts from Bulgarian herbs. Phytotherapy Res 20:961-965. doi:10.1002/ptr.1985   DOI
2 Baek Y.W. 1997, Micropropagation of Centella asiatica (L.) Urban by in vitro cultures and production of triterpene glycosides. PhD Dissertation, Chonnam National Univ., Gwangju, Korea, pp 13-15.
3 Brinkhaus B., M. Lindner, D. Schuppan, and E.G. Hahn 2000, Review article: chemical, pharmacological and clonical profile of the East Asian medical plant Centella asiatica. Phytomedicine 7:427-448. doi:10.1016/S0944-7113(00)80065-3   DOI
4 Zhao Y.L., H. Wei, H.H. Zheng, Z. Guo, Y.S. Wei, D.H. Zheng, and J. Zhang 2010, Enhancing water-solubility of poorly soluble drug, asiatic acid with hydrozypropyl-B-cyclodextrin. Dig J Nanomater Biostruct 5:419-425.
5 Zhimin Q, C. Xinxin, H. Jingbo, L. Qinmei, Y. Qinlei, Z. Junfeng, and D. Xuming 2017, Asiatic acid enhances Nrf2 signaling to protent HepG2cells fromoxidative damage through Akt and ERK activation. Biomed Pharmacother 88:252-259. doi:10.1016/j.biopha.2017.01.067   DOI
6 Valadabadi A.S., and A.H. Faranhani 2010, Effects of planning density and pattern on physiological growth indices in maize (Zea mays L.) under nitrogenous fertilizer application. J Agric Ext Rural Dev 2:40-47. doi:10.5897/JAERD.9000035   DOI
7 Hokmalipour S., and M.H. Darbandi 2011, Effects of nitrogen fertilizer on chlorophyll cotent and other leaf indicate in three cultivars of maize. World Appl Sci J 15:1780-1785.
8 Gohil K.J., J.A. Patel, and A.K. Gajjar 2010, Pharmacological review on Centella asiatica: a potential herbal cure-all. Indian J Pharm Sci 72:546-556. doi:10.4103/0250-474X.78519   DOI
9 Goo Y.M., Y.S. Kil, S.M. Sin, D.Y. Lee, W.M. Jeong, K. Ko, K. Yang, Y.H. Kim, and S.W. Lee 2018, Analysis of antibacterial, anti-inflammatory, and skin-whitening effect of Centella asiatica (L.) Urban. J Plant Biotechnol 45:117-124. (in Korean) doi:10.5010/JPB.2018.45.2.117   DOI
10 Hausen B.M. 1993, Centella asiatica (Indian pennywort), an effective therapeutic but a week sensitizer. Contact Derm 29:175-179. doi:10.1111/j.1600-0536.1993.tb03532.x   DOI
11 Marinova D., F. Ribarova, and M. Atanassova 2005, Total phenolics and total flavonoids in Bulgarian fruits and vegetables. J Univ Chem Technol Metall 40:255-260.
12 Hong J.J., H.G. Seol, J.Y. Oh, E.H. Jeong, and Y.H. Chang 2021, Effective component contents and antioxidative activities of unripe apple by extraction methods. Korean J Food Nutr 34:174-180. (in Korean) doi:10.9799/ksfan.2021.34.2.174   DOI
13 Lee S.H., S.H. Lee, S.K. Kim, E.Y. Hong, S.H. Chun, I.C. Son, and D.I. Kim 2014, Effect of harvest time on the several phenolic compounds and fruit quality of grape cultivars. Korean J Plant Res 27:119-124. (in Korean) doi:10.7732/kjpr.2014.27.2.119   DOI
14 Ong E.S., J.S.H. Cheong, and D. Goh 2006, Pressurized hot water extraction of bioactive or marker compounds in botanicals and medicinal plant materials. J Chromatogr A 1112:92-102. doi:10.1016/j.chroma.2005.12.052   DOI
15 Prasad A., S.S. Dhawan, A.K. Mathur, O. Prakash, M.M. Gupta, R.K. Verma, R.K. Lal, and A. Mathur 2014, Morphological, chemical and molecular characterization of Centella asiatica germplasms for commercial cultivation in the Indo-Gangetic plains. Nat Product Commun 9:779-784. doi:10.1177/1934578X1400900612   DOI
16 Stankovic M.S. 2011, Total phenolic content, flavonoid concentration and antioxidant activity of Marrubium peregrinum L. extracts. Kragujevac J Sci 33:63-72.
17 Ryu B.S., H.E. Choi, W.S. Choi, N.H. Lee, and U.K. Choi 2017, Antioxidant activities of extracts from different parts of the pine tree. Korean J Food Nutr 30:1133-1139. (in Korean) doi:10.9799/ksfan.2017.30.6.1133   DOI
18 Song F.L., R.Y. Gan, Y. Zhang, Q. Xiao, L. Kuang, and H.B. Li 2010, Total phenolic contents and antioxidant capacities of selected Chinese medicinal plants. Int J Mol Sci 11:2362-2372. doi:10.3390/ijms11062362   DOI
19 Tommasini S., D. Raneri, R. Ficarra, M.L. Calabro, R. Stancanelli, P. Ficarra 2004, Improvement in solubility and dissolution rate of flavonoids by complexation with β-cyclodextrin. J Pharm Biomed Anal 35:379-387. doi:10.1016/S0731-7085(03)00647-2   DOI
20 Madeira A.C., A. Ferreira, A. de Varennes, and M.I. Vieira 2003, SPAD meter versus tristimulus colorimeter to estimate chlorophyll content and leaf color in sweet papper. Commun Soil Sci Plant Anal 34:2461-2470. doi:10.1081/CSS-120024779   DOI
21 Gbolahan B.W.j., A.I. Abiola, J. Kamaldin, M.A. Ahmad, and M.S. Atanassova 2016, Accession in Centella asiatica; current understanding and future knowledge. J Pure Appl Microbiol 10:2485-2494. doi:10.22207/JPAM.10.4.02   DOI
22 Belwal T., H.C. Andola, M.S. Atanassova, B. Joshi, R. Suyal, S. Thakur, A. Bisht, A. Jantwal, O.D. Bhatt, and R.S. Rawal 2019, Chapter 3.22-Gotu Kola (Centella asiatica). In SM Nabavi, AS Silva, eds, Nonvitamin and nonmineral and nutritional supplements, Acadamic Press, pp 265-275.
23 Bonte F., M. Dumas, C. Chaudagne, and A. Meybeck 1994, Influence of asiatic acid, madecassic acid, and asiaticoside on human collagen I synthesis. Plant Med 60:133-135. doi:10.1055/s-2006-959434   DOI
24 Cho C.W., D.S. Choi, M.H. Cardone, C.W. Kim, A.J. Sinskey, and C. Rha 2006, Glioblastoma cell death induced by asiatic acid. Cell Biol Toxicol 22:393-408. doi:10.1007/s10565-006-0104-2   DOI
25 Mook-Jung I, J.E. Shin, S.H. Yun, K. Hur, J.Y. Koh, H.K. Park, S.S. Jew, and M.W. Jung 1999, Protective effects of asiaticoside derivatives against beta-amyloid neurotoxicity. J Neurosic Res 58:417-425. doi:10.102/(SICI)1097-4547(19991101)58.3<417::AID-JNR7>3.0.CO;2-G   DOI
26 Kwon M.C., J.G. Han, J.H. Ha, S.H. Oh, L. Jin, H.S. Jeong, G.P. Choi, B. Hwang, and H.Y. Lee 2008, Immuno-regulatory effect on Centella asiatica L. Urban extraction solvent associated with ultrasonification process. Korean J Medicinal Crop Sci 16:294-300. (in Korean)
27 Kim W.J., J. Kim, B. Veriansyah, J.D. Kim, Y.W.j Lee, S.G. Oh, and R.R. Tjandrawinata 2009, Extraction of bioactive components from Centella asiatica using subcritical water. J Supercrit Fluids 48:211-216. doi:10.1016/j.supflu.2008.11.007   DOI
28 Shin H.Y., H. Kim, E.J. Jeong, J.E. Kim, K.H. Lee, Y.J. Bae, and K.W. Yu 2020, Bioactive compounds, anti-oxidant activities, and anti-inflammatory activites of solvent extracts from Centella asiatica cultured in Chungju. Korean J Food Nutr 33:692-701. (in Korean) doi:10.9799/ksfan.2020.33.6.692   DOI
29 Zainol M.K., A. Abd-Hamid, S. Yusof, and R. Muse 2003, Antioxidative activity and total phenolic compounds of leaf, root and petiole of four accessions of Centella asiatica (L.) Urban. Food Chem 81:575-581. doi:10.1016/S0308-8146(02)00498-3   DOI
30 Liu M., Y. Dai, X. Yao, Y. Li, Y. Luo, Y. Xia, and Z. Gong 2008, Anti-rheumatoid arthritic effect of madecassoside on type II collagen-induced arthritis in mice. Int Immunopharmacol 8:1561-1566. doi:10.1016/j.intimp.2008.06.011   DOI
31 Suh J.T., K.D. Kim, H.B. Sohn, S.J. Kim, S.Y. Hong, and Y.H. Kim 2020, Comparative study of antioxidant activities at different cultivation area and harvest date of the Gomchwi 'Sammany' variety. Korean J Plant Res 33:245-254. (in Korean) doi:10.7732/kjpr.2020.33.4.245   DOI
32 Yun K.J., J.Y. Kim, J.B. Kim, K.W. Lee, S.Y. Jeong, H.J. Park, H.J. Jung, Y.W. Cho, K. Yun, and K.T. Lee 2008, Inhibition of LPS-induced NO and PGE 2 production by asiatic acid via NF-ĸB inactivation in RAW 264.7 macrophages: possible involvement of the IKK and MAPK pathways. Int Immunopharmacol 8:431-441. doi:10.1016/j.intimp.2007.11.003   DOI
33 Shukla A., A.M. Rasik, and B.N. Dhawan 1999, Asiaticoside-induced elevation of antioxidant levels in healing wounds. Phytother Res 13:50-54. doi:10.1002/(SICI)1099-1573(199902)13:1<50::AID-PTR368>3.0.CO;2-V   DOI
34 Zheng C.J. and L.P. Qin 2007, Chemical components of Centella asiatica and their bioactivities. J Chinese Integr Med 5:348-351. doi:10.3736/jcim20070324   DOI
35 Puttarak P., and P. Panichayupakaranant 2013, A new method for preparing pentacyclic triterpene rich. Natural Product Research 27:684-686. doi:10.1080/14786419.2012.686912   DOI