Browse > Article
http://dx.doi.org/10.5010/JPB.2020.47.4.298

Morphometric variation, genetic diversity and allelic polymorphism of an underutilised species Thaumatococcus daniellii population in Southwestern Nigeria  

Animasaun, David Adedayo (Department of Plant Biology, Faculty of Life Sciences, University of Ilorin)
Afeez, Azeez (Department of Plant Biology, Faculty of Life Sciences, University of Ilorin)
Adedibu, Peter Adeolu (Department of Plant Biology, Faculty of Life Sciences, University of Ilorin)
Akande, Feyisayo Priscilla (Department of Plant Biology, Faculty of Life Sciences, University of Ilorin)
Oyedeji, Stephen (Department of Plant Biology, Faculty of Life Sciences, University of Ilorin)
Olorunmaiye, Kehinde Stephen (Department of Plant Biology, Faculty of Life Sciences, University of Ilorin)
Publication Information
Journal of Plant Biotechnology / v.47, no.4, 2020 , pp. 298-308 More about this Journal
Abstract
Genetic diversity among Thaumatococcus daniellii populations in the southwestern region of Nigeria were assessed using morphometric and molecular markers to determine the population structure and existing genetic relationship for its improvement, conservation and sustainable utilisation. Populations from five locations in each of the six states were used for the study. Morphometric data were collected on folia characters and analysed for variability. Genome DNA was isolated from the plant leaf and amplified by polymerase chain reaction with inter-simple sequence repeat markers (ISSR) to determine the allelic polymorphism, marker effectiveness and genetic relationship of the population. The results showed significant variations in petiole length and leaf dimensions of the populations within and across the states. These morphometric traits are the major parameters that delimit the populations and they correlated significantly at P≤0.05. Analysis of the electrophoregram showed that the ISSR markers are effective for the diversity study. A total of 136 loci were amplified with an average of 7.16 loci per marker, 63.2% of the loci were polymorphic. The Principal Coordinate Analysis revealed that seven factors accounted for 81.6% of the variation and the dendrogram separated the populations into two major groups at a genetic distance of 10 (about 90% similarity) with sub-groups and clusters. Most populations within the state had a high degree of similarity, nonetheless, strong genetic relationship exists among populations from different states. The close relationship between populations across the states suggests a common progenitor, which are likely separated by ecological or geographical isolation mechanisms.
Keywords
Dendrogram; ecotypes; genetic diversity; morphological variation; percentage polymorphism; population structure;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Waliszewski WS, Sinclair FL, Steele KA (2012) Morphological and AFLP diversity in Thaumatococcus daniellii, the source of the protein sweetener thaumatin. Genet. Resour. Crop Evol. 59:151-161
2 Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res., 18:6531-6535
3 Zas R (2003) Foliar nutrient status and tree growth response of young Pseudotsuga menziesii Mirb. (Franco) to nitrogen, phosphorus and potassium fertilization in Galicia (Northwest Spain). Journal of Ecology, 12(1):75-85
4 Makinde SO, Taiwo CK (2004) In situ population evaluation of T. daniellii (Benth). ASSET Inter. J. 3:75-80
5 Ojekale AB, Makinde SCO, Osileye O (2007) Phytochemistry and antimicrobial evaluation of Thaumatococcus danielli Benn. (Benth.) leaves. Nigeria Food Journal, 25(2):176-183
6 Olatunji TL, Afolayan AJ (2019) Evaluation of genetic relationship among varieties of Capsicum annuum L. and Capsicum frutescens L. in West Africa using ISSR markers. Heliyon 5(2019):e01700
7 Onwueme IC, Onochie BE, Safowora EA (1979) Cultivation of T. daniellii the sweetener, World Crop. SAS Institute, 2012. SAS user's guide: Statistics SAS/C Online Doc, Release 9.20, SAS, Cary, NC, U.S.A. 106p
8 Oyedej S, Animasaun DA, Bello AA, gboola, OO (2014) Effect of NPK and Poultry manure on growth, yield and proximate composition of three Amaranths. Journal of Botany. (Vol. 2014) http://dx.doi.org/10.1155/2014/828750   DOI
9 Paterson AH, Tanksley SD, Sorrels ME (1991) DNA markers in plant improvement. Adv. Agron. 46:39-90
10 Pfeiffer T, Roschanski AM, PannellKorbecka G, Schnitter M (2011) Characterization of microsatellite loci and reliable genotyping in a polyploidy plant, Mercurialis perennis (Euphorbiaceae). The J. Hered. 102:479-488
11 Rauf S, da Silva JAT, Khan AA, Naveed A (2010) Consequences of plant breeding on genetic diversity. Int. J. Plant Breed. 41:1-21
12 Olorunmaiye KS, Joseph GG, Animasaun DA, Oyedeji S (2019) Mutagenic components and dosage effects of ethyl methanesulphonate on Arachis hypogea (SAMNUT 24 VR.). Ife J. Sci. 21(2):309-322
13 Gbadegesin A, Olabode MA (2000) Soil properties in the metropolitan region of Ibadan, Nigeria: implications for the management of the urban environment of developing countries. The Environmentalist. 20:205-214
14 Elemo BO, Adu OB, Alabi AM (2001) Isolation and partial purification of carbohydrate component of Thaumatococcus danielli (Benth.). Nig. J. Biochem. and Molecular Biol. 16: 87-90
15 Elemo BO, Adu OB, Ogunrinola OO, Efuwape TO, Olaleye KO, Kareem AA (2011) Biological evaluation of Thaumatococcus daniellii waste protein. Pakistan J. Nutri. 10(11):1048-1052
16 Esselink GD, Nybom H, and Vosman B (2004) Assignment of allelic configuration in polyploids suing the MAC-PR (Microsatellite DNA allele counting-peak ratios) method. Theor. Appl. Genet. 109:402-408
17 Falconer J (1990) The major significance of 'minor' forest products. The local use and value of forests in West African Humid Forest Zone. FAO, Rome. 59p
18 Foulds W (1993) Nutrient concentrations of foliage and soil in South-western Australia. New Phytologist, 125:529-546
19 Gunn S, Farrar JF, Collis BE, Nason M (1999) Specific leaf area in barley: individual leaves versus whole plants. New Phycologist. 143:45-51 41
20 Liu F, Liang X, Zhang S (2000) Accumulation and utilization efficiency of potassium in ramie varieties. J. Plant Nutr. 23:785-792
21 Mayland FH, Wilkinson SR (1989) Soil factors affecting magnesium availability in plant-animal systems: A Review. J. Anim. Sci. 67:3437-3444
22 Mohapatra A, Rout GR (2005) Identification and genetic variation among rose cultivars using random amplified polymorphic DNA. Z. Naturforsch., 60c:611-617
23 Thorn B (2004) Chefs find banana leaves appealing: natural foodwrapper, an authenticway to showcase tropical, ethnic foods. Nations Restaurant News, November 8, 2004
24 Davis RM, Coker G, Parfitt RL, Simcock R, Clinton PW, Garrett LG, Watt MS (2007) Relationships between soil and foliar nutrients in young densely planted mini-plots of Pinus radiata and Cupressus lusitanica. Forest Ecol. and Manag. 240(1-3): 122-130
25 Chinedu SN, Emiloju OC, Iheagwam FN, Rotimi SO, Popoola JO (2018) Phylogenetic relationship and genetic variation among Thaumatococcus daniellii and Megaphrynium macrostachyum ecotypes in Southwest Nigeria. Asian J. Plant Sci. 17:27-36
26 Rout GR, Senapati SK, Aparajita S (2007) Studies on the genetic relationship among 13 cultivars of Calathea (Marentheceae) using RAPD and ISSR markers. Adv. Hortic. Sci., 21:147-155
27 Sofowora A (1993) Medicinal plants and medicine in Africa. John Wiley spectrum books, Ibadan, Nigeria, Pp 120-123
28 Chinedu SN, Oluwadamisi AY, Popoola ST, David BJ, Epelle T (2014) Analyses of the leaf, fruit and seed of Thaumatococcus daniellii (Benth.): Exploring potential uses. Pak. J. Biol. Sci. 17:849-854
29 Cichorz S, Goska M, Litwiniec A (2014) Miscanthus: Genetic diversity and genotype identification using ISSR and RAPD markers. Mol. Biotechnol. 56:911-924
30 Csurhes S, Edwards R (1998) Potential environmental weeds in Australia: Candidate species for preventative control. Environ. Australia. 3(5):208-215
31 Rana M, Sharma R, Sharma P (2014) Estimation of genetic diversity in Capsicum annuum L. germplasm using PCR-based molecular markers. Natl. Acad. Sci. Lett. 37 (3):295-301
32 Ajibade SR, Weeden NF, Michite S (2000) Inter simple sequence repeat analysis of genetic relationships in the genus Vigna. Eupytical. 111:47-55
33 Boadi SM (2011) Ecology, conservation, cultivation and management potential of Thaumatococcus daniellii for agroforestry system. A PhD Thesis, School of Graduate Studies, Kwame Nkrumah University of Science and Technology, Kumasi Ghana Pp 56-71
34 Fagbemi AA, Sogunle EAA (1995) Nigeria: Reference soil of the moist lowlands near Ife (Oshun State). Soil Brief Nigeria 3. University of Ibadan, Ibadan and International Soil Reference and Information Centre, Wagneingen. Pp 13
35 Tadesse A (2017) Inter Simple Sequence Repeat (ISSR) Markers for Genetic Diversity Studies in Trifolium Species. Advan. Life Sci. and Technol. 55:34-37
36 Tyagi AK, Sharma MK, Surya MSK, Kerkhi SA, Chand P (2014) Estimates of genetic variability, heritability and genetic advance in linseed (Linum usitatissinum L.) germplasm. Prog. Agric. 14(1):37-48
37 Van Der Wel H, Loeve K (1972) Isolation and characterization of thaumatin I and 11, the Sweet -Tasting Proteins from Thaumatococcus daniellii Benth. Eur. J. Biochem. 31:221-225
38 Yeboah SO, Hilger TH, Kroschel J (2003) Thaumatococcus danielli (Benth): A natural sweetener from the rain forestzone in West Africa with potential income generation in small scale farming. J. Applied Sci. 6:854-859
39 Adebisi AO, Adeoye IB, Olajide FB, Usman JM, Agbarevoh P, Oyedele OO (2010) Gender analysis of production, potentials and constraints of Thaumatococcus daniellii. J. Bot. 1:3-5
40 Adeyemi TOA, Idowu OD, Ogboru RO, Iyebor WE, Owoeye EA (2014) Phytochemical screening, nutritional and medicinal benefits of Thaumatococcus daniellii Benn (Benth.). Inter. J. Appli. Res. and Technol. 3(8):92-97
41 Chen S, Pang X, Song J, Shi L, Yao H, Han J, Leon C (2014) A renaissance in herbal medicine identification: from morphology to DNA. Biotechnol. Adv. 32(7):1237-1244
42 Animasaun DA, Morakinyo JA, Mustapha OT, Krishnamurthy R (2015) Assessment of genetic diversity in accessions of pearl millet (Pennisetum glaucum) and napier grass (Pennisetum purpureum) using micro satellite (ISSR) markers. Iranian J. Genet. Plant Breed. 4(1):25-35
43 Animasaun DA, Morakinyo JA, Krishnamurthy R, Mustapha OT (2017) Genetic divergence of Nigerian and Indian pearl millet accessions based on agronomical and morphological traits. J. Agric. Sci. 62(2):115-131
44 Arowosoge OGE, Popoola L (2006) Economic analysis of Thaumatococcus daniellii (Benn.) Benth. (Miraculous berry) in Ekiti State, Nigeria. Food, Agric. and Environ. 4(1):264-269
45 Azeez MA, Olowookere MB, Animasaun DA, Bello BO (2017) Utility of some floral characters in the assessment of genetic diversity in sesame (Sesamum indicum L.). Acta Agriculturae Slovenica. 109(1):61-70
46 Boadi S, Baah-Acheamfour M, Ulzen-Appiah F, Jamro GM (2014) Nontimber forest product yield and income from Thaumatococcus daniellii under a mixed tree plantation system in Ghana. Int. J. Forest. Res. 2014(1). 10.1155/2014/524863.   DOI
47 Ogunsanwo OY, Adedeji GA, Ajibabi AS (2012) Pulping potential of Thaumatococcus daniellii (Benn) Benth in Omo and Oban Forest Reserve of Nigeria. I.J.S.N., 3(2):580-585
48 Most BH, Summerfield RJ, Boxall M (1978) Tropical plants with sweetening properties, Thaumatococcus daniellii. Econ. Bot. 32:321-335
49 Ndukwe AA, Enoh EA, Aniere QU (2014) Determination of morphological and nutritional properties of Thaumatococcus daniellii and effect of harvesting method on the plant growth. Inter. J. Innov. and Scienti. Res. 11(2):304-311
50 Nwachokor MA, Uzu FO (2008) Updated classification of some soil series in southwestern Nigeria. J. Agrono. 7(1):76-81
51 Swift KAD, Pearce S, Roth H (2002) Out of Africa: The Chemistry and flavour properties of the protein thaumatin. In: Swift, K. A. D. (ed). Advances in flavours and fragrances; from the sensation to the synthesis. Royal Society of Chemistry, UK Pages 178-193
52 Sotannde OA, Oluwadare AO (2014) Fibre and elemental contents of Thaumatococcus daniellii stalk and its implications as a non-wood fibre source. Inter. J. Appli Sci. and Technolo. 4(1):178-185
53 Stephens EL, Saha S, Menges ES (2009) Intracanopy variation in leaf morphology and physiology in dominant shrubs of Florida's xeric uplands. Botany, 87(1):112-125