• 생약학회지
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• 제1권2호
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• pp.23-28
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• 1970

One of the indigeneous plants to this country, Syringa dilatata $N_{AKAI}$ (Oleaceae) is known in commerce as 'Ya-Jung-Hyang' (野丁香) and has been known to be of effect as bitter stomachics. Seasonal variations in the content of polyalcohols and free reducing sugars in leaves of this plant which contains syringin glycoside, mannitol and free reducing sugars etc. were studied. Application of chromotropic acid to formaldehyde which was obtained from polyalcohols and reducing sugars by treatment with periodic acid results in reddish violet coloration and the solution has absorption maximum at wave length $570m\;{\mu}$. By the use of ionic exchange resin chromatography, poyalcohols were separated from the above mixture. The content of polyalcohols of this plant was plentiful in the growing season while that of free reducing sugars was decreasing vice versa.

• 한국임상수의학회지
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• 제15권1호
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• pp.171-173
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• 1998

A case of peritonitis caused by Actinomycotic spp is reported in a 12-year-old male Siberian tiger. Grossly, the mesentery was markedly thickened and contained numerous 1 to 3 mm diameter, white to yellowish foci. Fibrous adhesion showing tumorous thickening was also noted between the mesentery and abdominal organs. Histologicallyi the thickened mesentery and masses consisted of necrotic center with bacterial colonies surrounded by eosinophilic club (Splendore-hoeppli), neutrophils, macrophages, a few Iymphocytes and fibrosis. The bacterial colonies stained positvely with Gram's stain but were negative on acid-fast and periodic acid-Schifr method. Howeverr since the bacterial culture was not availablei the definitive causative agent was not able to specified.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2021년도 봄 학술논문 발표대회
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• pp.91-92
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• 2021

In the present work, we simulated and explained the bonding of three alkanethiols - hexanethiol (HT), decanethiol (DT), and 11-mercaptoundecanoic acid (MDA) - with Fe(110) surface and Fe2 clusters using Density Functional Theory (DFT) to probe the corrosion inhibition mechanisms. The interaction energies computed from periodic DFT calculations successfully predicted the experimental inhibition performance. We have found strong covalent bond formation between S(thiol) and Fe-atoms in both approaches, further confirmed by the projected density of states and electron density difference. Besides, natural bond orbital (NBO) charge distribution showed that DT had stronger electron-donation and back-donation synergic interactions with Fe-atoms.

• 한국가축번식학회지
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• 제22권4호
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• pp.331-339
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• 1998

본 연구는 Sertoli 세포 column 과 Sertoli 세포돌기 미세구조의 연구를 위해 당질검출을 위한 PA-TCH-SP, -PD 투과전자현미경용 특수염색을 정세관에 적용한 후 각 정세포와 Sertoli 세포를 관찰하고 비교하여 이 분야 연구에 본염색법의 적용 가능성을 검토하였다. PA-TCH-SP, -PD 특수염색을 개의 정세관에 적용하여 투과전자현미경으로 관찰하였던 바, Sertoli 세포 column 과 돌기 부위에 수많은 반응과립이 관찰되었으며 정세관 기저부의 정조세포질에서도 약간의 반응과립이 관찰되었다. 이와 같은 반응과립은 각 정세포의 핵, 정모세포질, 정자세포질 및 잔류체에는 존재하지 않아 반응과립이 존재하는 Sertoli 세포 colunm 과 돌기 및 정자세포질에 침투된 돌기들을 명확히 구분할 수 있었다. 따라서 PA-TCH-SP, -PD 염색법은 투과전자현미경하에서 Sertoli 세포 colunm 과 돌기의 형태학적 및 기능학적 연구에 대단히 유용한 염색법으로 사료된다.

• 디지털융복합연구
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• 제12권6호
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• pp.433-438
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• 2014

본 연구는 흰쥐의 정소에 방사선을 조사하였을 때 시간이 경과함에 따라 나타나는 정소세포 및 정자의 변화를 현미경학적으로 얻은 영상을 기초로 영상처리에 의한 정량분석을 통하여 정자의 변화에 대한 결과를 정량화함으로써 더 정확하고 객관적인 결과를 얻고자 하는데 그 목적을 두고 있다. 연구에는 8주 수령의 흰쥐를 대상으로 하였으며, 6 MV의 X선을 1회 2 Gy를 전신 조사하였다. 총 30 마리의 흰쥐를 대상으로 방사선을 조사하지 않은 정상대조군과 실험군을 조사 즉시, 조사 2시간, 4시간, 8시간, 24시간 후에 각 실험군 마다 5마리의 정소를 각각 적출하였다. 적출 후 Periodic acid Schiff 염색을 시행하여 정상대조군과 실험군에서의 정소세포와 정자의 상태를 관찰하였다. 방사선조사 후 24시간까지, 정소세포수와 정자수가 점차 감소하는 것을 정성적 및 정량적으로 유의함을 확인하였다.

• 환경생물
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• 제22권3호
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• pp.387-393
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• 2004

사료를 통한 benzo(a)pyrene이 조피볼락, Sebastes schlegeli의 lipid peroxidation (LPO), superoxide dismutase(SOD)활성과 간 조직의 병변 현상에 미치는 영향을 연구하기위해 0 (대조구), 0.5, 1.0, 1.5, 2.0 mg $kg^{-1}$ 농도의 사료를 조제하여 30일간 급이하였다. LPO는 2.0mg kg$^{-1}$농도구에서 전 실험기간 동안 유의적으로 증가하였고, SOD 활성도 2.0 mg $kg^{-1}$ 농도구에서 30일째에 유의적으로 증가하였다. 간 조직 검경시 10일째에 1.0mg $kg^{-1}$ 이상의 농도구에서 세포비대가 나타났고, 같은 농도에서 periodic acid-Schiff (PAS) 염색시 양성으로 나타나는 granule이 30일째에 나타났고, 2.0mg $kg^{-1}$ 농도구에서는 20일째부터 관찰되었다. 그리고, 30일째 2.0mg $kg^{-1}$ 농도구의 일부 어체에서는 간 세포의 괴사가 관찰되었다.

• 한국포장학회지
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• 제26권1호
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• pp.19-23
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• 2020

Al-laminated packaging film incorporating ascorbic acid or tocopherol at inner food contact layer was tested in the potential to improve antioxidative preservation of powdered infant formula under CO₂-enriched atmosphere. Product of 200 g was packaged with the packaging film containing 0.3% antioxidant in sealant layer of low density polyethylene and stored at 30℃ for 286 days with periodic measurement of package atmosphere and product's quality attributes. The CO₂-flushed package resulted in shrinkage of tight contact between the product and the film not allowing gas sampling of package atmosphere after 140 days. Package of tocopherol-incorporated film allowed some ingress of oxygen after 112 days presumably due to its weakening of heat-seal area. The increased oxygen concentration in the tocopherol-added film package led to the concomitant increase of peroxide value, an index of lipid oxidation. On the other hand, packaging of ascorbic acid-added film pouch could suppress lipid oxidation marginally in consistent manner compared to control package without any antioxidant.

• Journal of Microbiology and Biotechnology
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• 제16권7호
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• pp.1047-1052
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• 2006

A bile salt hydrolase (BSH) was purified from Lactobacillus plantarum CK 102 and its enzymatic properties were characterized. This enzyme was successfully purified using ion-exchange chromatography with Q-Excellose and hydrophobic interaction chromatography with Butyl-Excellose. The purified enzyme showed a single protein band of 37 kDa by SDS-polyacrylamide gel electrophoresis, which was similar to the molecular weight of known BSHs. The amino acid sequence of GLGLPGDLSSMSR, determined by MALDI-TOF, was identical to that of BSH of L. plantarum WCFS1. Although this BSH hydrolyzed all of the six major human bile salts, glycine-conjugated bile acid was the best substrate, based on its specificity and $K_{m}$ value. Among the various substrates, the purified enzyme maximally hydrolyzed glycocholate with apparent $K_{m}$ and $V_{max}$ values of 0.5 mM and 94 nmol/min/mg, respectively. The optimal pH of the enzyme ranged from 5.8 to 6.3. This enzyme was strongly inhibited by thiol enzyme inhibitors such as iodoacetate and periodic acid.

• 한국식품과학회지
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• 제23권6호
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• pp.689-694
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• 1991

우리나라 신라시대를 전후하여 일반적인 청량음료로 사용되었던 것으로 알려진 장수(?水)의 제조방법을 제민요술에 근거하여 재현하고 그 발효학적 특징을 규명 하려 하였다. 새로지은 뜨거운 쌀밥을 항아리에 담고 찬물을 부어 3일간 상온에서 발효한 후 윗물과 밥 절반을 떠낸 후 다시 새로지은 뜨거운밥과 찬물을 채워 넣는 일을 주기적으로 반복하면서 미생물균총의 조성을 관찰하였다. 밥과 물을 혼합하여 실온에 방치하면 3일 동안 총균수, 단백 질 분해균, 산생성균수가 지수적으로 증가하나 그 이후부터 다소 감소하며 6일 후에는 단백질 분해균수가 증가하여 부패현상을 나타낸다. 그러나 장수 제조법에 의하면 단백질 분해균이 크게 감소하고 산생성균이 지배균총이 된다. 장수에서 분리한 주요 산생성균은 Lactobacillus, Lactococcus, Pediococcus, Leuconostoc spp.였으며 이중 주요균으로 Lactococcus thermophilus, Lactobacillus coryniformis 및 Leuconostoc mesenterioides를 잠정 동정하였다. 분리한 유산균 들을 starter로 사용하여 장수를 제조한 결과 산생산력이 크고 풍미가 우수한 제품을 얻을 수 있었다.

• 동국한의학연구소논문집
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• 제2권2호
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• pp.69-84
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• 1993

This study was performed to identify the effect of Backchulsan and Backpeundu, on the muco-substances of the goblet cells in the small and large intestines of the mouse. After injected castor oil into oral cavity, the experimental animals were administrated with 5.952mg per 30g body weight of Backchulsan, with 0.519mg per 30g body weight of Backpeundu. The specimens used in this study were obtained from the small and large intestines of the mouse. All specimens were stained with periodic acid-Schiff reaction, alcian blue pH 2.5 and alcian blue pH 1.0 procedures. The results were as follows : 1. The most remarkable decrease of neutral and acid mucosubstance was observed in the control group 1 and 6 hours after castor oil administration. 2. Increase of neutral and mucosubstance was observed in Backchulsan administration group 3 hours in comparision with that of control group administrated of castor oil, especially of the upper and lower villi regions. 3. Increase of neutral and acid mucosubstance was observed in Backpeundu administration group 6 hours in comparision with that of control group administration of castor oil. 4. The most remarkable increase of neutral and acid mucosubstance was observed in Backchulsan administration group after 3 hours in comparision with that of Backpeundu administration group.