Journal of Digital Convergence (디지털융복합연구)

The Society of Digital Policy and Management (한국디지털정책학회)
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The quantitative analysis by the image processing of sperm changes according to the radiation irradiation of white rat testicle

흰쥐 정소의 방사선 조사에 따른 정자변화의 영상처리에 의한 정량분석

  • Na, Soo-Kyung (Dept. of Radiological Science, Gimcheon University) ;
  • Kim, Sung-In (Dept. of Biomedical Laboratory Science, Gimcheon University) ;
  • Lee, On-Seok (Dept. of Radiological Science, Gimcheon University)
  • 나수경 (김천대학교 간호보건대학 방사선학과) ;
  • 김성인 (김천대학교 간호보건대학 임상병리학과) ;
  • 이언석 (김천대학교 간호보건대학 방사선학과)
  • Received : 2014.03.20
  • Accepted : 2014.06.20
  • Published : 2014.06.28
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Abstract

This study aims to get more accurate and objective result by quantifying the result for the sperm changes through the quantitative analysis by the image processing based on the image obtained microscopically for the testicle cell and sperm change appeared with the passage of time when the radiation is irradiated to the white rat testicle. This study has targeted the white rat of 8 weeks lifespan, the X-ray of 6 MV with 1 time of 2 Gy has been irradiated to the whole body. The testicles of 5 rats at each test group immediately after irradiation, after 2 hours of irradiation, 4 hours, 8 hours and 24 hours has been respectively extracted targeting all 30 white rats of normal control group not irradiated by the radiation and the test group. The state of testicle cell and sperm has been observed in the normal control group and the test group by implementing Periodic acid Schiff dyeing after extraction. 24 hours after irradiation, a gradual decrease in sperm count and testicular cells qualitatively and quantitatively that were identified as significant.

본 연구는 흰쥐의 정소에 방사선을 조사하였을 때 시간이 경과함에 따라 나타나는 정소세포 및 정자의 변화를 현미경학적으로 얻은 영상을 기초로 영상처리에 의한 정량분석을 통하여 정자의 변화에 대한 결과를 정량화함으로써 더 정확하고 객관적인 결과를 얻고자 하는데 그 목적을 두고 있다. 연구에는 8주 수령의 흰쥐를 대상으로 하였으며, 6 MV의 X선을 1회 2 Gy를 전신 조사하였다. 총 30 마리의 흰쥐를 대상으로 방사선을 조사하지 않은 정상대조군과 실험군을 조사 즉시, 조사 2시간, 4시간, 8시간, 24시간 후에 각 실험군 마다 5마리의 정소를 각각 적출하였다. 적출 후 Periodic acid Schiff 염색을 시행하여 정상대조군과 실험군에서의 정소세포와 정자의 상태를 관찰하였다. 방사선조사 후 24시간까지, 정소세포수와 정자수가 점차 감소하는 것을 정성적 및 정량적으로 유의함을 확인하였다.

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References

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