• The Korean Journal of Food And Nutrition
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• v.32 no.6
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• pp.669-677
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• 2019

The impact of storage temperature on the changes in acid value(AV), peroxide value(POV), color value, total phenolic content(TPC), and antioxidant activity in perilla seed(PS) was investigated. The PS was stored at 25, 35, and 45℃ for four weeks. An increase in the storage temperature resulted in significantly increased AV, POV, redness, and yellowness of the PS. Conversely, TPC, antioxidant activity, and redness of the PS significantly decreased. The changes in the AV and POV followed a first-order kinetic model, and the kinetic parameters such as k, t_1/2, Q₁₀ and Ea were calculated. The k and t_1/2 values decreased with increasing storage temperature and the Q₁₀ values for the AV and POV were 1.56, 1.91, 4.61, and 3.43, respectively. The Ea for the changes in of the AV and POV in the PS were 70.40, 102.63 kJ/mol, respectively. The half-life values for the AV and POV of the PS at 25℃ were 169.52 and 373.18 days, respectively, while the values at 45℃ for those, were 28.47 and 27.93 days, respectively.

• Korean Journal of Plant Resources
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• v.30 no.3
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• pp.298-310
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• 2017

Morphological variation between cultivated and weedy types of Perilla frutescens var. frutescens and P. frutescens var. crispa were studied in 327 germplasm by examining 17 morphological characters. The germplasm between the two varieties were varied for their qualitative and quantitative characters. The seed coat color of cultivated P. frutescens var. frutescens is commonly light brown and brown while deep brown color was observed in the weedy type P. frutescens var. frutescens and P. frutescens var. crispa. The leaf size, cluster length, plant height, flower number per cluster and seed weight in cultivated P. frutescens var. frutescens were significantly (P<0.05) different from weedy type P. frutescens var. frutescens and P. frutescens var. crispa. The cultivated P. frutescens var. frutescens exhibited significantly higher plant height (158.6 cm) compared to the weedy P. frutescens var. crispa (133.8 cm). Likewise, seed weight was significantly higher in cultivated (1.9 g) than in the weedy type of P. frutescens var. frutescens (1.6 g) and P. frutescens var. crispa (1.4 g). Principal component analysis (PCA) result showed that the first and second principal component cumulatively explained 86.6% of the total variation. The cultivated type P. frutescens var. frutescens and its weedy accessions were not clearly separated with P. frutescens var. crispa by PCA. Hence it requires the use of molecular markers for better understanding of their genetic diversity.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.54 no.3
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• pp.270-278
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• 2009

To understand the seed characteristics among cultivated types of Perilla crop and their weedy types in Korea and Japan, we studied the variation of 69 accessions by examining weight of 100 seeds, hardness of seed, germination percent and germination energy. The survey of the weight of 100 seeds and hardness of seed, clarified as follows; cultivated type of var. frutescens showed a range of 0.177 to 0.402 g with weight of 100 seeds, and most accessions of cultivated type of var. frutescens have soft seeds, except for several accessions, which have hard seeds, whereas weedy type of var. frutescens showed a range of 0.045 to 0.172 g with weight of 100 seeds, and has only hard seeds. While cultivated type of var. crispa showed a range of 0.054 to 0.101 g with weight of 100 seeds, and has only hard seeds. The weedy type of var. crispa showed a range of 0.059 to 0.135 g with weight of 100 seeds, and has only hard seeds. According to the result of germination tests, although the most accessions of cultivated and weedy types of var. frutescens and cultivated and weedy types of var. crispa showed below 50% germination rate at the first germination test, but some accessions of cultivated type of var. frutescens showed above 50% germination. While, in the second or third germination tests, most accessions of cultivated types of Perilla crop and their weedy types showed above 50% germination rates, except for several accessions. As a result, in this study, the average of germination percent and germination energy among accessions of cultivated types of Perilla crop and their weedy types showed respectively the highest value at the third germination test, but showed the lowest value at the first germination test. In addition, the germination percent and germination energy showed much higher in the room temperature condition than in $28^{\circ}C$ constant-temperature condition. Although our results may be required much clearly survey in the further study, this current results will be help for our understanding the variation of seed characteristics among cultivated types of Perilla crop and their weedy types in Korea and Japan.

• Korean Journal of Plant Resources
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• v.18 no.1
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• pp.179-185
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• 2005

This study was conducted to evaluate the major agronomic characteristics Korean of 85 and Chinese Jilim Province of 5 local varieties of perilla at Chungbuk Agricultural Research and Extension Services in 2001. Perilla accessions examined were classified into three maturity groups, i.e, early, medium, and late maturity group of $19\;(23\%),\;57\;(67\%)$, and $9\;(10\%)$ accessions, respectively. The early and late maturity groups mainly consisted of collections from middle-northern area and southern area, respectively, while the medium group consisted of accessions from all over the country. But chinese Jilim collection wad all mediumgroup. Average 1,000-seed weight(TSW) was 2.7 g. TSW of the collection from Hamyang was the greatest a 3.9g, while the TSW of Pyungchang collection was the smallest as 1.7g, and most of collections produced medium and small seeds. Perilla accessions with greate 1,000-seed weight seemed to be belonged to the late maturity group. Chinese collection was 2.2 to 3.19g and overage was 2.6g in average. Accodingly TSW hadn't more variegation than Korean. Seed coat colors of gorilla accessions were dark brown$(30\%)$, brown$(55\%)$ and gray brown$(6\%)$, respectively. Among seed coat colors, brown color consisted of $91\%$. Chinese was dark brown$(20\%)$, brown$(80\%)$ in color. Positively correlated that stem height, the number of nodes, branches and flower clusters with Korean and Chinese collection, capsules per flower cluster and the length of flower cluster with Korean collection and 1,000-seed weight and maturing period, flower clusters, stem height and the number of nodes with Chinese collection. And negatively correlated that the number of capsules per flower cluster and 1,000-seed weight with Korean collection and the length of flower cluster, stem height, branches, the number of nodes and flower clusters with Chinese collection.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.5
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• pp.420-424
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• 2006

This experiment was conducted to elucidate the basic physiological phenomena of pollination and fertilization for breeding in perilla (Perilla frutescens) through different 6 varieties derived from 2 genera under 4 temperature conditions at day and night. The pollen germination was observed after 30 minutes and pollen tube reached the lower part of pistil after 1 hour from artificial pollination. Seed formation was affected by temperature condition and a variety. The early maturing variety 'YCPL 25' showed a poor seed formation rate under the night temperature below $15^{\circ}C$, but the late maturing variety 'YCPL263' did not lowered even though the night time temperature was $10^{\circ}C$. The function of pistil was long maintained under the low temperature from the fact that if the live pollen pollinate artificially to the flower that does not form seed under low temperature, fruiting was made. Pollen was created and pollen tube was developed in the time of petal becomes bigger than calyx in five varieties, 'YCPLl77-1' etc. However, pollen was made and pollen tube was made only 71% in a green chajogi 'YCPL205-1'. These physiological phenomena of pollination and fertilization could be applicable to the emasculation and an effective breeding in perilla.

• Korean Journal of Food Science and Technology
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• v.46 no.1
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• pp.61-67
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• 2014

This study aimed to investigate the anti-inflammatory activities of raw (P) and roasted (RP) Perilla frutescens Britton (perilla) seeds in RAW 264.7 macrophages and an ulcerative colitis mouse model. In lipopolysaccharide-treated RAW 264.7 cells, treatment with ethanol extract of P at the concentrations of 75 and $150{\mu}g/mL$ decreased nitric oxide (NO), interleukin-6 (IL-6), and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) levels to 48-85% of the control (p<0.01). Treatment with RP extract exhibited similar effects on NO, IL-6, and TNF-${\alpha}$, decreasing those levels to 51-84% of the control (p<0.01). In dextran sulfate sodium-treated ulcerative colitis mice, dietary treatment with 1% RP for 7 days decreased the colonic levels of prostaglandin $E_2$ and leukotriene $B_4$ to 34% and 58% of the control, respectively (p<0.05). Dietary P treatment, however, did not decrease those levels significantly. These results indicate that roasted perilla seed exerts anti-inflammatory activity both in vitro and in vivo.

• Korean Journal of Plant Resources
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• v.16 no.2
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• pp.155-159
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• 2003

This study was conducted to examine the variations in 196 local perilla collected in southern part of Korea and to provide basic informations which can be utilize in perilla breeding programs. The results obtained were gummarized as the follows. Maturing period was widely distributed from 104 to 136 days. The maturing periods of most collections were 111 to 130 days(96%). and that Four percent of collections showed maturing period of below 110 days and above 131 days. Three varieties below 111 days of maturing period were selected among local perilla collected from Jeonnam(Naju and Boseong) and Jeonbuk(Gochang). Stem length was ranged from 71 to 157 cm. The stem length of 111 to 130 cm, under 90 cm, and above 151 cm were 41％,, 7％, and 4％, respectively. Seven varieties with long stem were selected among local perilla collected in Jeonnam(include Damyang collection). The number of cluster per plant was distributed 52 to 291. The distribution of cluster numbers were 100 to 180, under 100, and above 221 was 57％, 13% , and 13%, respectively. The number of seed capsules was distributed 23 to 56 and the collections ranged from at of 26 to 45 was 49.5％. Seed cluster length was distributed from 6 to 20 cm and that ranged from 8 to 14 cm was 62.8%.1,000 grain weight ranged from 2.4 to 5.7 g. The distribution of 1,000 grain weight is 3.1 to 4.0 g,4.1 to 5.0 g, and below 2.9 g was 66.8%, 12.8%, and 19.4%, respectively. The 1,000 grain weight above 5.1 g(1.0%) was selected among local perilla collected in Jeonbuk(Namwon) and Gyeongnam(Changnyeong).

• Korean Journal of Plant Resources
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• v.28 no.1
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• pp.135-144
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• 2015

The Korean daily intake of vegetable oils has increased about 2.5-fold from 17 g/day to 46 g/day for the last several decades. Perilla (Perilla frutescens var. frutescens) has been cultivated in Korea for a long time as a dietary oil seed which has the highest content of ${\alpha}$-linolenic acid, accounting for nearly 60%. It is known that the main role of ALA is as a precursor to the longer-chain ${\omega}-3$, eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), the metabolic products of ${\alpha}$-linolenic acid (ALA, ${\omega}-3$). Dietary ${\omega}-3$ fatty acids reduce inflammation and the risk of chronic diseases such as heart disease, cancer, and arthritis, but they also may act as functional components for cognitive and behavioral function. Thus, ${\alpha}$-linolenic acid is one of the essential nutrients in modern dietary patterns in which much linoleic acid is consumed. Nevertheless, perilla oil, rich in ${\alpha}$-linolenic acid, can be easily oxidized, giving rise to controversies with respect to shelf life, the deterioration of the product's commercial value, and further related toxicity. Recent research using genetic modifications has tried to develop new plant oil seeds that balance the ratio of ${\omega}-6/{\omega}-3$ fatty acids. Such trials could be a strategy for improving an easily oxidizable property of perilla oil due to high ${\alpha}$-linolenic acid. Alternatively, appropriate application of antioxidant to the oil can be considerable.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.391-396
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• 2009

Perilla frutescens, which belonging to the Labiatae family, is widely cultivated oil crop and have been used traditional herbal medicine in East Asia such as Korea, China, and Japan. Especially, the leaves and the seeds of this species are important in Korean traditional cooking, as one of the popular garnish and food colorants. Numerous studies have revealed that the beneficial health effects of perilla are due to its several phytochemicals contents, such as rosmarinic acid, caffeic acid, luteolin, and apigenin. For this reason, increasing the content of phytochemicals in perilla hasbecome a major breeding objective. The genetic diversity of the rosmarinic acid, caffeic acid, luteolin, and apigenin content in perilla seed is poorly documented. We analyzed the rosmarinic acid, caffeic acid, luteolin, and apigenin content of 203 accessions of perilla germplasm by high performance liquid chromatography (HPLC). The rosmarinic acid and luteolin contents ofgermplasms were ranged from $15.7{\mu}g/g$ to $2717.1{\mu}g/g$ and from $1.6{\mu}g/g$ to $582.4{\mu}g/g$ respectively.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.58 no.4
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• pp.408-415
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• 2013

To better understand the morphological variation of the Perilla crop and their weedy types in East and Southeast Asia, we studied the morphological variation of 90 accessions by examining 10 morphological characteristics, such as flowering time, seed size, seed hardness, seed color, color of surface leaf, color of reverse side leaf etc. As a result, morphological variation determined that between cultivated var. frutescens and var. crispa, and between cultivated var. frutescens and its weedy type showed significant morphological differences in terms of seed size and seed hardness, whenever cultivated var. crispa and its weedy type could not showed significant differences in most morphological characters. In PCAs (principal component analysis), among 10 morphological characteristics, flower color (QL6), color of surface leaf (QL3), seed size (QN2), seed hardness (QL1), seed color (QL2), stem color (QL7), and color of reverse side leaf (QL4) contributed in negative direction on the first axis, while flowering time (QN1), leaf shape (QL5), and degree of pubescence (QL8) contributed in positive direction on the first axis. Among these morphological characters, particularly flower color (QL6), color of surface leaf (QL3), seed size (QN2), seed hardness (QL1), and degree of pubescence (QL8) were useful characters for discrimination between cultivated var. frutescens and weedy var. crispa, and between cultivated var. frutescens and its weedy type. However, most accession of cultivated and weedy types of var. crispa was not clearly discriminated by PCA analyses. Although the wild ancestral species of var. frutescens and of var. crispa are still unknown in East and Southeast Asia, the weedy types of Perilla crop may be the key taxon for our understanding of the origin of cultivated types of var. frutescens and var. crispa.