• Title/Summary/Keyword: Pericarp

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Sequential Changes of Pericarp Ultrastructure in Citrus reticulata Hesperidium (Citrus reticulata 감과 과피 내 미세구조 변화)

  • Kim, In-Sun
    • Applied Microscopy
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    • v.33 no.1
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    • pp.79-92
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    • 2003
  • Ultrastructural changes of the pericarp in Citrus reticulata has been investigated during hesperidium abscission. The pericarp was composed of compactly arranged parenchyma cell layers during early stages of fruit development. The outermost exocarp was green and active in photosynthesis. However, cells in the exocarp soon changed into collenchyma cells by developing unevenly thickened walls within a short time frame. As the fruit approached maturation, the chlorophyll gradually disappeared and chloroplasts were transformed into carotenoid-rich chromoplasts. In the mature fruit the exocarp consisted of large, lobed collenchyma cells with primary pit fields and numerous plasmodesmata. The immature mesocarp was a relatively hard and thick layer, located directly under the exocarp. With development, the deeper layers of the exocarp merged into the white, spongy mesocarp. Before separation of the hesperidium from the plant, some unusual features were detected in the plasma membrane of the exocarp cells. The number of small vacuoles and dark, irregular osmiophilic lipid bodies also increased enormously in the exocarp collenchyma after the abscission. They occurred between the plasma membrane and the wall, and invaginated pockets of the plasma membrane containing double-membraned vesicles were also frequently noticed. The lipid bodies in the cytoplasm were often associated with other organelles, especially with plastids and mitochondria. The plastids, which were irregular or amoeboid in shape, contained numerous large lipid droplets, and occasional clusters of phytoferritin, as well as few loosely -oriented peripheral lamellae. Myelin-like configurations of membrane were frequently observed in the vacuoles, as was the association of lipid bodies with the vacuolar membrane. Most vacuoles had an irregular outline, and lipid bodies were often connected to the tonoplast of the vacuoles. The structural changes underlying developmental, particularly to senescence, processes in various hesperidium will be reported in the separate paper.

Electron Microscopic Study for the Influence of Soaking in Hot Water and Prochloraz Solution on Spore and Mycelium of Fusarium fujikuroi Infected in Rice Seed (온탕소독과 prochloraz 침지소독이 벼 종자에 감염된 Fusarium fujikuroi의 포자와 균사의 형태에 미치는 영향에 대한 전자현미경적 연구)

  • Park, Woo-Sik;Yeh, Wan-Hae;Lee, Se-Weon;Han, Seong-Suk;Lee, Jun-Seong;Lim, Chun-Keun;Lee, Yong-Hwan
    • Research in Plant Disease
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    • v.14 no.3
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    • pp.176-181
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    • 2008
  • This experiment was conducted to find the causes of ineffective seed disinfection methods such as rice seeds soaking in hot water and prochloraz EC solution when the rice seeds were severely infected by Bakanae disease. In case of rice seeds collected from severely diseased field by Bakanae disease, the pathogen as the forms of spores and mycelium were infected in plumule and inner and outer integument of embryo, aleurone layer, and pericarp layer. When the rice seeds were soaked in hot water, the appearances of spores and hypha on the outer pericarp layer were severely disordered, however those of inner region of outer integument and aleurone layer were shown normal. The membrane of hypha on the outer pericarp layer was destroyed within 24 hours, while some spores were healthy and germinated 7 days after soaking, when the rice seeds soaked 24 hours in 125 ppm prochloraz solution at $30^{\circ}C$. These results indicated that the seed disinfection methods were ineffective on the Bakanae disease severely infected rice seed because the hot water did not transmit the pericarp layer of rice seed and also prochloraz solution did not effectively destroy the spore of pathogen.

Composition of Phenolic Acids and Flavonoids and Skin Care Cosmetic Antioxidant Activity of Akebia quinata Fruit Extracts (연복자 추출물의 Phenolic acids와 Flavonoids의 조성 및 화장품 항산화 활성)

  • Jang, Ah-Ram
    • Journal of Digital Convergence
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    • v.19 no.4
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    • pp.365-371
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    • 2021
  • In order to verify the effect of using functional cosmetic ingredients for skin beauty, the composition and content of some phenolic acid and flavonoids in the hot water and 80% methanol extract of Akebia quinata fruit pericarp and seeds were analyzed, and the skin care antioxidant activity was investigated. The results are as follows. Total polyphenol and flavonoid contents were found to be higher in pericarp extract than seed extract in both hot water and 80% methanol extract. In the analysis of the composition and content of phenolic acid and flavonoids, two kinds of phenolic acids were identified in the hot water extract of pericarp, 6 kinds of phenolic acids were identified in the 80% methanol extract, and one kind of flavonoid. Two types of phenolic acids were identified in the hot water and 80% methanol extract of seeds, respectively. DPPH and ABTS radical scavenging activities tended to increase in proportion to the treatment concentration in both hot water and 80% methanol extracts, and antioxidant activity was found to be high. Therefore, from the above results, it was found that the hot water and 80% methanol extract of Akebia quinata fruit pericarp and seeds contained various kinds of phenolic acid and flavonoids, and the antioxidant activity was also high. It is believed to be of value as a natural antioxidant in skin care cosmetic ingredients.

Study on waxy corn - XIII. Botanical characteristics and crude incomes analysis of Daedukchal 1 (찰옥수수 연구 - XIII. 다수성 찰옥수수 "대덕찰 1호"의 주요 작물학적 특성 및 경제성 분석)

  • Lee, Hee-Bong;Lee, Moon-Sub;Bok, Tae-Gyu;Choi, Yun-Pyo;Cha, Hui-Jung;Hong, Bum-Yong;Kim, Hyo-Gi
    • Korean Journal of Agricultural Science
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    • v.38 no.1
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    • pp.1-6
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    • 2011
  • Daeduckchal 1 waxy corn hybrid including check was planted and compared with domestic leading vatiety on agronomic characteristics, traits related to table gualities and crude income. Stem and ear height of Daeduckchal 1 among used hybrids were intermediate and stem diameter was also similar to other hybrids. Days to tasseling of this hybrid needed 56 days which was slightly shorter than check hybrid, Yeonnong. Ear length of CNU-H2 was longer than Yeonnongchal as 21.7 cm, while Chalok 2 was 14.1 cm. Number of tillers per plant of this hybrid were higher than Chalok 2 but lower than Yeonnong. Pericarp thickness of CNU-H2 and CNU-H3 were similar to Yeonnon. In sugar content, Daeduckchal 1 was intermediate level as 11.7 brix(%). Marketability of Daeduckchal 1 was the highest among used hybrids and crude incomes was also high.

Ear and Kernel Characteristics of Korean Indigenous Maize Lines Collected in Pusan and Kyungnam (부산, 경남지역에서 수집된 한국 재래종 옥수수의 이삭 및 낟알의 특성)

  • 이인섭;박종옥
    • Journal of Life Science
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    • v.11 no.2
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    • pp.159-165
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    • 2001
  • In order to reserve abundant germplasm for breeding new corn varieties, major characteristics of ears and kernels were evaluated with a total of 210 Korean indigenous maize lines collected from various parts of Pusan City and Kynugnam Province, Korea The average ear length and ear diameter of indigenous maize lines collected was 12.52cm and 3.33 cm, respectively. The average ear weight of the maize lines was 63.70g. The ears collected from the north-west mountainous region were the heaviest, and The ears from the south coastal region were the lightest. The average kernel weight per ear was 50.54g, and the kernel weight per ear by region showed a tendency similar to the ear weight. The kernels of maize lines collected in the north-west mountainous region were the longest, and kernel width and thickness were the largest in the west plain region. 100 kernel weight and embryo weight the largest in the lines collected in the north-west mountainous region. The degree of pericarp thickness was the smallest in the lines collected in the south coastal region, and largest in the lines collected in the west plain region. Except for the correlation coefficient width and 100 kernel weight, all correlation coefficients between the characteristics of the lines showed highly significant differences.

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Bran structure and some properties of waxy rice starches (찹쌀의 겨층 구조 및 전분의 몇가지 성질)

  • Kim, Sung-Kon;Sohn, Jung-Woo
    • Applied Biological Chemistry
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    • v.33 no.2
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    • pp.105-108
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    • 1990
  • The numbers of aleurone layers and thickness of pericarp of waxy rice in dorsal side were higher than those in vental side, However, varietal characteristics of the bran structures were observed. The water uptake rates of brown rice at $60^{\circ}C$ were similar between $j{\times}indica$ varieties(Hangangchalbyeo, H and Baegunchalbyeo, B) and higer than that of japonica variety (Shinsunchalbyeo, S). Inherent viscosities for H, B and S waxy rice starches were 1.92, 1.84 and 1.73 $dlg^{-1}$, respectively. The minimum moistures for gelatinization of waxy rice starches determined by DSC were $36.4{\sim}38.6%$ which represented 4moles of water per mole of hexose unit.

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Effects of Irradiation and Fumigation on Physicochemical Properties of Red Pepper during Storage (감마선과 훈증처리가 건고추의 저장 중 이화학적 특성에 미치는 영향)

  • Kim, Byeong-Keun;Kwon, Joong-Ho
    • Journal of the Korean Society of Food Culture
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    • v.19 no.5
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    • pp.491-498
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    • 2004
  • In order to study the quarantine and sanitization methods for dried red pepper, comparative effects of commercial fumigation (methyl bromide/MeBr, $phosphine gas/PH_{3}$ and gamma irradiation (5, 10 kGy) were investigated in terms of its physicochemical properties. There were no noticeable chances in pH and soluble solids among the untreated control, irradiated and fumigated samples soon after treatments, but some decrease was found in stored samples (especially soluble solid in fumigated samples) for 8 months under room temperature. Total sugar content was influenced by storage time rather than both treatments. Immediately after treatments, reducing sugar content was significantly reduced in the samples including pericarp when exposed to fumigants (p<0.05), while an apparent decrease was observed in the stored samples including seeds with negligible differences among treatment groups. The electron donating ability (EDA) of the extracts was high in the order of pericarp, whole pepper, powdered pepper and seeds, which was reduced during storage for 8 months particularly in the samples containing seeds. The EDA of irradiated samples during storage was equal to that of the control sample, whereas that of fumigated samples was relatively low (p<0.05).

Induction of Apoptotic Cell Death by Red Pericarp Rice (Jakwangchalbyeo) Extracts

  • Chi, Hee-Youn;Lee, Chang-Ho;Kim, Kwang-Ho;Kim, Sun-Lim;Chung, Ill-Min
    • Food Science and Biotechnology
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    • v.15 no.4
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    • pp.534-542
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    • 2006
  • The effects of ethanol fractions of three different rice grain extracts, Jakwangchalbyeo, Hwasunchalbyeo, and Ilpumbyeo, on apoptotic cell death in the rat hepatoma H4IIE cell line were investigated using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] cell viability assay. One hundred mg/mL Jakwangchalbyeo extract significantly reduced cell viability to 69.5, 57.2, and 46.1% within 24, 48, and 72 hr, respectively. Fluorescence-activated cell sorting (FACS) analyses were also performed to characterize the cell death pattern caused by treatment with the rice grain extracts. Apoptotic cell death was clearly observed with time after treatment with the Jakwangchalbyeo extract. In Western blotting analysis, degradation of the 116 kDa poly-ADP-ribose polymerase (PARP) molecule was observed with concomitant formation of an 89 kDa product 24, 48, and 72 hr after treating cells with the Jakwangchalbyeo extract. This indicates that an apoptotic process caused cell death in these cells. In conclusion, red-pericarp Jakwangchalbyeo extract induced apoptotic cell death in H4IIE cells to a larger extent than the other rice extracts.

Variation for Fruit Yield and Quality Characteristics in Sweet Pepper (Capsicum annuum L.) Germplasm Collection

  • Luitel, Binod Prasad;Lee, Taek Jong;Kang, Won Hee
    • Korean Journal of Breeding Science
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    • v.43 no.2
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    • pp.139-144
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    • 2011
  • Fifty-five sweet pepper (Capsicum annuum L.) germplasm from the Rural Development Administration (RDA) were analyzed for their fruit yield and fruit quality characters, including fruit weight, fruit size, pericarp thickness, total soluble content, fruit lobe, fruit shape, fruit surface and fruit color. Fruit yield per plot ranged from 200 g to 8150 g (average, 1884 g). Fruit fresh-weight per fruit ranged from 27.3 g to 200.0 g with an average of 97.2 g. Fruit length varied from 4.0 cm to 16.2 cm (average, 9.6 cm). Fruit length-to-width ratio ranged from 0.6 to 3.0 with an average 1.7. The fruit pericarp thickness ranged from 1.8 mm to 10.7 mm (average, 4.8 mm). Total soluble content was the highest ($8.5^{\circ}Brix$) in K156286 and the lowest ($5.3^{\circ}Brix$) in K156208 with the average value $6.7^{\circ}Brix$. Fruit lobes numbers varied from 2 to 4. Blocky, triangular, elongated, rectangular and round fruit shape identified among the sweet pepper accessions. Fruit surface varied from smooth to wrinkle and fruit color also varied. The presence of variability in fruit yield and quality traits within the sweet pepper genotypes can be utilized to develop high yielding sweet pepper variety with better fruit quality characters.