• Parasites, Hosts and Diseases
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• 제59권2호
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• pp.167-171
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• 2021

Haemonchosis remains a significant problem in small ruminants. In this study, the assay of recombinase polymerase amplification (RPA) combined with the lateral flow strip (LFS-RPA) was established for the rapid detection of Haemonchus contortus in goat feces. The assay used primers and a probe targeting a specific sequence in the ITS-2 gene. We compared the performance of the LFS-RPA assay to a PCR assay. The LFS-RPA had a detection limit of 10 fg DNA, which was 10 times less compared to the lowest detection limit obtained by PCR. Out of 24 goat fecal samples, LFS-RPA assay detected H. contortus DNA with 95.8% sensitivity, compared to PCR, 79.1% sensitivity. LFS-RPA assay did not detect DNA from other related helminth species and demonstrated an adequate tolerance to inhibitors present in the goat feces. Taken together, our results suggest that LFS-RPA assay had a high diagnostic accuracy for the rapid detection of H. contortus and merits further evaluation.

• Journal of Veterinary Science
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• 제23권4호
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• pp.51.1-51.10
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• 2022

Background: Due to the unavailability of an effective vaccine or antiviral drug against the African swine fever virus (ASFV), rapid diagnosis methods are needed to prevent highly contagious African swine fever. Objectives: The objective of this study was to establish the ladder-shape melting temperature isothermal amplification (LMTIA) assay for the detection of ASFV. Methods: LMTIA primers were designed with the p72 gene of ASFV as the target, and plasmid pUC57 was used to clone the gene. The LMTIA reaction system was optimized with the plasmid as the positive control, and the performance of the LMTIA assay was compared with that of the commercial real-time polymerase chain reaction (PCR) kit in terms of sensitivity and detection rate using 200 serum samples. Results: Our results showed that the LMTIA assay could detect the 10⁴ dilution of DNA extracted from the positive reference serum sample, which was the same as that of the commercial real-time PCR kit. The coincidence rate between the two assays was 100%. Conclusions: The LMTIA assay had high sensitivity, good detection, and simple operation. Thus, it is suitable for facilitating preliminary and cost-effective surveillance for the prevention and control of ASFV.

• 한국통신학회논문지
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• 제2권1호
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• pp.35-42
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• 1977

低周波增幅을 C級以上의 效率로 實現할 수 있는 스위칭 모우드 增幅器의 性能을 解析하였고 變調器로 使用하기 위한 回路構成과 얻어지는 特性改善에 關하여 考察하였다. 그 結果 變調度 97〔%〕까지의 直線性, 7,200〔Hz〕까지의 平坦한 周波數特性을 確認할 수 있었다.

• Journal of Dairy Science and Biotechnology
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• 제34권1호
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• pp.43-49
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• 2016

Emulsion PCR (ePCR) has recently gained interest in the areas of food safety and biotechnology owing to its highly specific and sensitive performance in the amplification of target DNA. To facilitate the applications of ePCR to food safety and biotechnology, this paper describes the principles of ePCR and the factors that should be considered in designing ePCR. In addition, current research and applications related to ePCR are discussed.

• 한국정밀공학회지
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• 제24권12호
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• pp.88-94
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• 2007

A flexure hinge-based compliant stage driven by stack-type piezoelectric elements has high precision motion but small operational range due to the characteristics of the piezoelectric element. Since the common flexure hinges can be broken by excessive deflection when the displacement is amplified by a high amplification ratio, a flexure hinge mechanism for large deflection is required. A cartwheel-type flexure hinge has an advantage of larger deflection compared with the common flexure hinges. This study presents a rotation stage with cartwheel-type flexure hinges driven by a stack-type piezoelectric element. The characteristics and the performance of the rotation stage are described by the terms of principal resonance frequency, amplification ratio of rotational displacement, maximum rotational displacement and block moment, in which the terms are analyzed by geometric parameters of the rotation stage. The analyzed results will be used as the guideline of the design of the rotation stage.

• Journal of Biosystems Engineering
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• 제35권5호
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• pp.323-329
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• 2010

Physical damages on fruits may be caused by shock and vibration inputs that transmitted from the transporting vehicle through the packaging and cushioning materials to the fruit. In this study, both half sine shock test and trapezoidal shock test were performed by MIL-STD-810F specification in order to investigate and represent the shock response properties such as peak acceleration and shock amplification factors of the pear according to packaging and cushioning materials for optimal packaging design during transportation. Shock excitation data that had been measured on the vehicle operating on the real road were used. Shock response properties measured by half sine shock test were smaller than those measured by trapezoidal shock test. Results represent that corrugation shapes and thickness can significantly affect the cushioning performance than the paper configurations of cushioning pad and showed that fruits may be damaged seriously while transported on the unpaved road without the properly cushioned packaging practices.

• 한국지진공학회논문집
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• 제22권2호
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• pp.87-94
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• 2018

In this study, seismic performance of bushings and their connection parts was analyzed by performing shaking table tests for various types of bushings widely used as auxiliary equipment of main transformers in domestic substations. As a result of the seismic tests of five types of 154 kV bushings according to the manufacturers, all the bushings secured the structural integrity even at the acceleration of 1.4 g and it was found that leakage of insulating oil didn't occur. Also, the average acceleration amplification rate at the upper part of the bushings was about 2.5 to 3.0 times higher than the lower one. On the other hand, when a representative 345 kV bushing was subjected to the seismic test, the structural integrity was secured even at 1.0 g acceleration similar to the design earthquake load level, but in this test, leakage of insulating oil occurred. However, when a stiffener restricting the connection of the bushing is installed in the same 345 kV bushing, the displacement of the bushing connection is controlled and the stiffener prevent the oil from leaking even at the acceleration of the designed seismic level.

• 한국광학회:학술대회논문집
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• 한국광학회 1995년도 광학 및 양자전자학 워크샵 논문집
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• pp.185-189
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• 1995

A high-power Nb:glass laser system(Simmyung I) has been contructed and tested. In this system, we used a Nb:YLF laser as a master oscillator, a 4-pass amplifier for pre-amplification, 5 stages of rod amplifiers, and spatial filtering and image reaying usits. The system has demonstrated in excess of 80J(2TW) with 40 psec(FWHM) pulse duration. Output energy, gain and spatial were measured at each amplification stage. With this laser system a preliminary X-ray generation experiment was performed. Pinhole images, X-ray diode signals and X-ray speriment were obtained for the irradiated target of copper. Detailed descriptions of the system performance and the X-tay generation experiment are presented.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2004년도 추계학술대회 논문집
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• pp.1353-1359
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• 2004

There are many massive components added on the railway overhead-line. These components cause larger fluctuations of contact forces, which are due to wave reflections and Doppler effects when a high-speed train passes those. In this paper, mathematical formula are derived for the relation between the added mass and contact force fluctuations. Using the derived formula, we calculate a added mass on the overhead-line which cause amplification factor to become 2.5. German design practice requires that amplification factor due to the wave reflection should be less than 2.5 to obtain good current collection performance. To show the validity of the formula, simulation results are compared with the calculation results. Simulation results showed that contact force fluctuations grow rapidly when an added mass is larger than the calculation result. Therefore, the simple form of formula can be used for estimating maximum added mass not to cause large fluctuations of contact forces in early design phase.

• 한국통신학회논문지
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• 제30권1A호
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• pp.1-9
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• 2005

표준 단일모드 광섬유를 전송선으로 사용하고, Raman 증폭기 없이 EDFA 증폭기만을 사용하여, 각 채널당 10 Gb/s의 파장다중화된 40개 채널신호들을 장거리 전송하는 경제적인 광 링크를 구성한 뒤, 이 링크의 전송 성능 최적화를 위해 분산 맵 구조의 차이에 따른 성능 차이를 분석하였다. 이 링크의 분산 맵은 전치분산보상, 구간분산보상 그리고 후치분산보상으로 나누어지는데 이들의 다양한 조합을 통해 15 가지의 다양한 분산 맵들을 구성한 뒤 각각의 경우를 분석하여 링크 성능을 크게 향상시키는 최적화된 분산맵 구조를 구하였다. 또한, 이 최적화된 분산 조건이, 단일모드 광섬유와 분산보상 광섬유가 가지는 파장에 따른 분산 기울기의 불일치로 인해 받게 되는 영향을 조사하였고, 이 영향이 링크의 성능에 미치는 효과가 무시될 수 있는 분산 기울기의 허용 가능한 불일치 범위를 구했다.