Browse > Article
http://dx.doi.org/10.4142/jvs.22001

Development of a ladder-shape melting temperature isothermal amplification (LMTIA) assay for detection of African swine fever virus (ASFV)  

Wang, Yongzhen (Key Laboratory of Biomarker Based Rapid-Detection Technology for Food Safety of Henan Province, Xuchang University)
Wang, Borui (School of Food and Biological Engineering, Henan University of Science and Technology)
Xu, Dandan (School of Food and Biological Engineering, Henan University of Science and Technology)
Zhang, Meng (School of Food and Biological Engineering, Henan University of Science and Technology)
Zhang, Xiaohua (Key Laboratory of Biomarker Based Rapid-Detection Technology for Food Safety of Henan Province, Xuchang University)
Wang, Deguo (Key Laboratory of Biomarker Based Rapid-Detection Technology for Food Safety of Henan Province, Xuchang University)
Publication Information
Journal of Veterinary Science / v.23, no.4, 2022 , pp. 51.1-51.10 More about this Journal
Abstract
Background: Due to the unavailability of an effective vaccine or antiviral drug against the African swine fever virus (ASFV), rapid diagnosis methods are needed to prevent highly contagious African swine fever. Objectives: The objective of this study was to establish the ladder-shape melting temperature isothermal amplification (LMTIA) assay for the detection of ASFV. Methods: LMTIA primers were designed with the p72 gene of ASFV as the target, and plasmid pUC57 was used to clone the gene. The LMTIA reaction system was optimized with the plasmid as the positive control, and the performance of the LMTIA assay was compared with that of the commercial real-time polymerase chain reaction (PCR) kit in terms of sensitivity and detection rate using 200 serum samples. Results: Our results showed that the LMTIA assay could detect the 104 dilution of DNA extracted from the positive reference serum sample, which was the same as that of the commercial real-time PCR kit. The coincidence rate between the two assays was 100%. Conclusions: The LMTIA assay had high sensitivity, good detection, and simple operation. Thus, it is suitable for facilitating preliminary and cost-effective surveillance for the prevention and control of ASFV.
Keywords
African swine fever (ASF); African swine fever virus (ASFV); ladder-shape melting temperature isothermal amplification (LMTIA); commercial real-time PCR kit;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Li C, He X, Yang Y, Gong W, Huang K, Zhang Y, et al. Rapid and visual detection of African swine fever virus antibody by using fluorescent immunochromatography test strip. Talanta. 2020;219:121284.   DOI
2 Aira C, Ruiz T, Dixon L, Blome S, Rueda P, Sastre P. Bead-based multiplex assay for the simultaneous detection of antibodies to African swine fever virus and classical swine fever virus. Front Vet Sci. 2019;6(6):306.   DOI
3 Liu J, Shi H, Cong G, Chen J, Zhang X, Shi D, et al. Development of a rapid and sensitive europium (III) chelate microparticle-based lateral flow test strip for the detection and epidemiological surveillance of porcine epidemic diarrhea virus. Arch Virol. 2020;165(5):1049-1056.   DOI
4 Lin Y, Cao C, Shi W, Huang C, Zeng S, Sun J, et al. Development of a triplex real-time PCR assay for detection and differentiation of gene-deleted and wild-type African swine fever virus. J Virol Methods. 2020;280:113875.   DOI
5 Zuo L, Song Z, Zhang Y, Zhai X, Zhai Y, Mei X, et al. Loop-mediated isothermal amplification combined with lateral flow dipstick for on-site diagnosis of African swine fever virus. Virol Sin. 2021;36(2):325-328.   DOI
6 Ceruti A, Kobialka RM, Ssekitoleko J, Okuni JB, Blome S, Abd El Wahed A, et al. Rapid extraction and detection of African swine fever virus DNA based on isothermal recombinase polymerase amplification assay. Viruses. 2021;13(9):1731.   DOI
7 Zeng D, Qian B, Zhao K, Qian Y, Dai J. Rapid on-site detection of African swine fever virus using polymerase chain reaction with a lateral flow strip. Microchem J. 2020;156:104940.   DOI
8 Bai J, Lin H, Li H, Zhou Y, Liu J, Zhong G, et al. Cas12a-based on-site and rapid nucleic acid detection of African swine fever. Front Microbiol. 2019;10:2830.   DOI
9 Yu LS, Chou SY, Wu HY, Chen YC, Chen YH. Rapid and semi-quantitative colorimetric loop-mediated isothermal amplification detection of ASFV via HSV color model transformation. J Microbiol Immunol Infect. 2021;54(5):963-970.   DOI
10 Chen D, Wang D, Wang C, Wei F, Zhao H, Lin X, et al. Application of an AlphaLISA method for rapid sensitive detection of African swine fever virus in porcine serum. Appl Microbiol Biotechnol. 2021;105(11):4751-4759.   DOI
11 Mee PT, Wong S, O'Riley KJ, da Conceicao F, Bendita da Costa Jong J, Phillips DE, et al. Field verification of an African swine fever virus loop-mediated isothermal amplification (LAMP) assay during an outbreak in timor-leste. Viruses. 2020;12(12):1444.   DOI
12 Wang Z, Yu W, Xie R, Yang S, Chen A. A strip of lateral flow gene assay using gold nanoparticles for point-of-care diagnosis of African swine fever virus in limited environment. Anal Bioanal Chem. 2021;413(18):4665-4672.   DOI
13 Miao F, Zhang J, Li N, Chen T, Wang L, Zhang F, et al. Rapid and sensitive recombinase polymerase amplification combined with lateral flow strip for detecting African swine fever virus. Front Microbiol. 2019;10:1004.   DOI
14 Tran HT, Dang AK, Ly DV, Vu HT, Hoang TV, Nguyen CT, et al. An improvement of real-time polymerase chain reaction system based on probe modification is required for accurate detection of African swine fever virus in clinical samples in Vietnam. Asian-Australas J Anim Sci. 2020;33(10):1683-1690.   DOI
15 Njau EP, Machuka EM, Cleaveland S, Shirima GM, Kusiluka LJ, Okoth EA, et al. African swine fever virus (ASFV): biology, genomics and genotypes circulating in sub-Saharan Africa. Viruses. 2021;13(11):2285.   DOI
16 Pikalo J, Deutschmann P, Fischer M, Roszyk H, Beer M, Blome S. African swine fever laboratory diagnosis-lessons learned from recent animal trials. Pathogens. 2021;10(2):177.   DOI
17 Zhang X, Liu X, Wu X, Ren W, Zou Y, Xia X, et al. A colloidal gold test strip assay for the detection of African swine fever virus based on two monoclonal antibodies against P30. Arch Virol. 2021;166(3):871-879.   DOI
18 Chen C, Lai H, Liang H, He Y, Guo G, Li L. A new method for detection African swine fever virus: timeresolved fluorescence immunoassay. J Fluoresc. 2021;31(5):1291-1296.   DOI
19 Liu H, Shi K, Sun W, Zhao J, Yin Y, Si H, et al. Development a multiplex RT-PCR assay for simultaneous detection of African swine fever virus, classical swine fever virus and atypical porcine pestivirus. J Virol Methods. 2021;287:114006.   DOI
20 Yuan F, Petrovan V, Gimenez-Lirola LG, Zimmerman JJ, Rowland RR, Fang Y. Development of a blocking enzyme-linked immunosorbent assay for detection of antibodies against African swine fever virus. Pathogens. 2021;10(6):760.   DOI
21 Jia L, Jiang M, Wu K, Hu J, Wang Y, Quan W, et al. Nanopore sequencing of African swine fever virus. Sci China Life Sci. 2020;63(1):160-164.   DOI
22 Kostyusheva A, Brezgin S, Babin Y, Vasilyeva I, Glebe D, Kostyushev D, et al. CRISPR-Cas systems for diagnosing infectious diseases. Methods 2021. Epub ahead of print. doi: 10.1016/j.ymeth.2021.04.007.   DOI
23 Wang D, Wang Y, Zhang M, Zhang Y, Sun J, Song C, et al. Ladder-shape melting temperature isothermal amplification of nucleic acids. Biotechniques. 2021;71(1):358-369.   DOI
24 Fraczyk M, Wozniakowski G, Kowalczyk A, Niemczuk K, Pejsak Z. Development of cross-priming amplification for direct detection of the African swine fever virus, in pig and wild boar blood and sera samples. Lett Appl Microbiol. 2016;62(5):386-391.   DOI
25 Wozniakowski G, Fraczyk M, Kowalczyk A, Pomorska-Mol M, Niemczuk K, Pejsak Z. Polymerase crosslinking spiral reaction (PCLSR) for detection of African swine fever virus (ASFV) in pigs and wild boars. Sci Rep. 2017;7(1):42903.   DOI
26 Kokkinos PA, Ziros PG, Bellou M, Vantarakis A. Loop-mediated isothermal amplification (LAMP) for the detection of Salmonella in food. Food Anal Methods. 2014;7(2):512-526.   DOI
27 Wang X, Ji P, Fan H, Dang L, Wan W, Liu S, et al. CRISPR/Cas12a technology combined with immunochromatographic strips for portable detection of African swine fever virus. Commun Biol. 2020;3(1):62.   DOI
28 Tao D, Liu J, Nie X, Xu B, Tran-Thi TN, Niu L, et al. Application of crispr-cas12a enhanced fluorescence assay coupled with nucleic acid amplification for the sensitive detection of African swine fever virus. ACS Synth Biol. 2020;9(9):2339-2350.   DOI
29 Wang D, Yu J, Wang Y, Zhang M, Li P, Liu M, et al. Development of a real-time loop-mediated isothermal amplification (LAMP) assay and visual LAMP assay for detection of African swine fever virus (ASFV). J Virol Methods. 2020;276:113775.   DOI
30 Ren M, Mei H, Zhou M, Fu ZF, Han H, Bi D, et al. Development of a super-sensitive diagnostic method for African swine fever using CRISPR techniques. Virol Sin. 2021;36(2):220-230.   DOI
31 Ye X, Li L, Li J, Wu X, Fang X, Kong J. Microfluidic-CFPA chip for the point-of-care detection of African swine fever virus with a median time to threshold in about 10 min. ACS Sens. 2019;4(11):3066-3071.   DOI