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Emulsion PCR Improves the Specificity and Sensitivity of PCR-based Pathogen Detection  

Chai, Changhoon (Division of Applied Animal Science, Kangwon National University)
Publication Information
Journal of Dairy Science and Biotechnology / v.34, no.1, 2016 , pp. 43-49 More about this Journal
Abstract
Emulsion PCR (ePCR) has recently gained interest in the areas of food safety and biotechnology owing to its highly specific and sensitive performance in the amplification of target DNA. To facilitate the applications of ePCR to food safety and biotechnology, this paper describes the principles of ePCR and the factors that should be considered in designing ePCR. In addition, current research and applications related to ePCR are discussed.
Keywords
polymerase chain reaction; emulsion PCR; microdroplets; pathogen detection; PCR sensitivity;
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